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Ramazan H Yilmaz - One of the best experts on this subject based on the ideXlab platform.
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a novel Antioxidant Agent caffeic acid phenethyl ester prevents long term mobile phone exposure induced renal impairment in rat
Molecular and Cellular Biochemistry, 2005Co-Authors: Fehmi Ozguner, Ahmet Koyu, Faruk Oktem, Ali Ayata, Ramazan H YilmazAbstract:Caffeic acid phenethyl ester (CAPE), a flavonoid like compound, is one of the major components of honeybee propolis. It has been used in folk medicine for many years in Middle East countries. It was found to be a potent free radical scavenger and Antioxidant recently. The aim of this study was to examine long-term applied 900 MHz emitting mobile phone-induced oxidative stress that promotes production of reactive oxygen species (ROS) and, was to investigate the role of CAPE on kidney tissue against the possible electromagnetic radiation (EMR)-induced renal impairment in rats. In particular, the ROS such as superoxide and nitric oxide (NO) may contribute to the pathophysiology of EMR-induced renal impairment. Malondialdehyde (MDA, an index of lipid peroxidation) levels, urinary N-acetyl-β-d-glucosaminidase (NAG, a marker of renal tubular injury) and nitric oxide (NO, an oxidant product) levels were used as markers of oxidative stress-induced renal impairment and the success of CAPE treatment. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in renal tissue were determined to evaluate the changes of Antioxidant status. The rats used in the study were randomly grouped (10 each) as follows: i) Control group (without stress and EMR), ii) Sham-operated rats stayed without exposure to EMR (exposure device off), iii) Rats exposed to 900 MHz EMR (EMR group), and iv) A 900 MHz EMR exposed + CAPE treated group (EMR + CAPE group). In the EMR exposed group, while tissue MDA, NO levels and urinary NAG levels increased (p < 0.0001), the activities of SOD, CAT, and GSH-Px in renal tissue were reduced (p < 0.001). CAPE treatment reversed these effects as well (p < 0.0001, p < 0.001 respectively). In conclusion, the increase in NO and MDA levels of renal tissue, and in urinary NAG with the decrease in renal SOD, CAT, GSH-Px activities demonstrate the role of oxidative mechanisms in 900 MHz mobile phone-induced renal tissue damage, and CAPE, via its free radical scavenging and Antioxidant properties, ameliorates oxidative renal damage. These results strongly suggest that CAPE exhibits a protective effect on mobile phone-induced and free radical mediated oxidative renal impairment in rats.
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the activities of tissue xanthine oxidase and adenosine deaminase and the levels of hydroxyproline and nitric oxide in rat hearts subjected to doxorubicin protective effect of erdosteine
Toxicology, 2003Co-Authors: Ersin Fadillioglu, Ramazan H Yilmaz, Hasan Erdogan, Sadik SogutAbstract:Abstract The aim of this experimental study was to investigate the effects of erdosteine, an Antioxidant Agent, on doxorubicin (DXR)-induced cardio-toxicity through nitric oxide (NO) levels, collagen synthesis, xanthine oxidase (XO) and adenosine deaminase (ADA) activities in rats. Rats were treated with erdosteine (10 mg/kg b.wt. per day, orally) or saline starting 2 days before administrating a single dose of DXR (20 mg/kg i.p.) or saline. At the 10th day of the DXR administration, hearts were removed under anesthesia for biochemical measurements. Enzyme activities as well as OH-proline and NO levels were found to be significantly increased in DXR group compared with the control group. All of the parameters studied except ADA activity were decreased significantly approximating to the control levels upon erdosteine administration. In conclusion, erdosteine seems to be an alternative Agent for protection of cardiac tissue against DXR-induced cardio-toxicity through its regulatory effect on XO activity and NO level.
Fehmi Ozguner - One of the best experts on this subject based on the ideXlab platform.
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a novel Antioxidant Agent caffeic acid phenethyl ester prevents long term mobile phone exposure induced renal impairment in rat
Molecular and Cellular Biochemistry, 2005Co-Authors: Fehmi Ozguner, Ahmet Koyu, Faruk Oktem, Ali Ayata, Ramazan H YilmazAbstract:Caffeic acid phenethyl ester (CAPE), a flavonoid like compound, is one of the major components of honeybee propolis. It has been used in folk medicine for many years in Middle East countries. It was found to be a potent free radical scavenger and Antioxidant recently. The aim of this study was to examine long-term applied 900 MHz emitting mobile phone-induced oxidative stress that promotes production of reactive oxygen species (ROS) and, was to investigate the role of CAPE on kidney tissue against the possible electromagnetic radiation (EMR)-induced renal impairment in rats. In particular, the ROS such as superoxide and nitric oxide (NO) may contribute to the pathophysiology of EMR-induced renal impairment. Malondialdehyde (MDA, an index of lipid peroxidation) levels, urinary N-acetyl-β-d-glucosaminidase (NAG, a marker of renal tubular injury) and nitric oxide (NO, an oxidant product) levels were used as markers of oxidative stress-induced renal impairment and the success of CAPE treatment. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in renal tissue were determined to evaluate the changes of Antioxidant status. The rats used in the study were randomly grouped (10 each) as follows: i) Control group (without stress and EMR), ii) Sham-operated rats stayed without exposure to EMR (exposure device off), iii) Rats exposed to 900 MHz EMR (EMR group), and iv) A 900 MHz EMR exposed + CAPE treated group (EMR + CAPE group). In the EMR exposed group, while tissue MDA, NO levels and urinary NAG levels increased (p < 0.0001), the activities of SOD, CAT, and GSH-Px in renal tissue were reduced (p < 0.001). CAPE treatment reversed these effects as well (p < 0.0001, p < 0.001 respectively). In conclusion, the increase in NO and MDA levels of renal tissue, and in urinary NAG with the decrease in renal SOD, CAT, GSH-Px activities demonstrate the role of oxidative mechanisms in 900 MHz mobile phone-induced renal tissue damage, and CAPE, via its free radical scavenging and Antioxidant properties, ameliorates oxidative renal damage. These results strongly suggest that CAPE exhibits a protective effect on mobile phone-induced and free radical mediated oxidative renal impairment in rats.
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mobile phone induced myocardial oxidative stress protection by a novel Antioxidant Agent caffeic acid phenethyl ester
Toxicology and Industrial Health, 2005Co-Authors: Fehmi Ozguner, Ahmet Altinbas, Mehmet Ozaydin, Abdullah Dogan, Huseyin Vural, Nesimi A Kisioglu, Gokhan Cesur, Nurhan Gumral YildirimAbstract:: Electromagnetic radiation (EMR) or radiofrequency fields of cellular mobile phones may affect biological systems by increasing free radicals, which appear mainly to enhance lipid peroxidation, and by changing the Antioxidant defense systems of human tissues, thus leading to oxidative stress. Mobile phones are used in close proximity to the heart, therefore 900 MHz EMR emitting mobile phones may be absorbed by the heart. Caffeic acid phenethyl ester (CAPE), one of the major components of honeybee propolis, was recently found to be a potent free radical scavenger and Antioxidant, and is used in folk medicine. The aim of this study was to examine 900 MHz mobile phone-induced oxidative stress that promotes production of reactive oxygen species (ROS) and the role of CAPE on myocardial tissue against possible oxidative damage in rats. Thirty rats were used in the study. Animals were randomly grouped as follows: sham-operated control group (N: 10) and experimental groups: (a) group II: 900 MHz EMR exposed group (N: 10); and (b) group III: 900 MHz EMR exposed+CAPE-treated group (N: 10). A 900 MHz EMR radiation was applied to groups II and III 30 min/day, for 10 days using an experimental exposure device. Malondialdehyde (MDA, an index of lipid peroxidation), and nitric oxide (NO, a marker of oxidative stress) were used as markers of oxidative stress-induced heart impairment. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities were studied to evaluate the changes of Antioxidant status. In the EMR exposed group, while tissue MDA and NO levels increased, SOD, CAT and GSH-Px activities were reduced. CAPE treatment in group III reversed these effects. In this study, the increased levels of MDA and NO and the decreased levels of myocardial SOD, CAT and GSH-Px activities demonstrate the role of oxidative mechanisms in 900 MHz mobile phone-induced heart tissue damage, and CAPE, via its free radical scavenging and Antioxidant properties, ameliorates oxidative heart injury. These results show that CAPE exhibits a protective effect on mobile phone-induced and free radical mediated oxidative heart impairment in rats.
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a novel Antioxidant Agent caffeic acid phenethyl ester prevents shock wave induced renal tubular oxidative stress
Urological Research, 2005Co-Authors: Fehmi Ozguner, Abdullah Armagan, Ahmet Koyu, Sadettin Caliskan, Halis KoyluAbstract:The aim of this study was to evaluate the effects of the novel free radical scavenger caffeic acid phenethyl ester (CAPE) on extracorporeal shock wave lithotripsy (ESWL) induced renal impairment. The study was performed using 30 rabbits which were divided into two groups, each exposed to 3,000 shock waves at 18 kV: (1) control group, (2) ESWL+CAPE treated group. Malodialdehyde (MDA), urine N-acetyl-β-glucosaminidase (NAG) activity, uric acid and white cell counts were used as markers of oxidative stress. Following shock wave exposure there was a significant rise in MDA, NAG and uric acid and white cell counts. CAPE reduced the rise in MDA, NAG, uric acid and white cell counts. Thus CAPE treatment to a great extent prevented the induction of these renal changes. Our results suggest that the Antioxidant capacity of the kidney tissue was reduced after ESWL treatment and that the tissue was exposed to oxidant stress. We conclude that CAPE treatment provided significant protection against ESWL induced free radical damage.
Dipali Sharma - One of the best experts on this subject based on the ideXlab platform.
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honokiol activates amp activated protein kinase in breast cancer cells via an lkb1 dependent pathway and inhibits breast carcinogenesis
Breast Cancer Research, 2012Co-Authors: Arumugam Nagalingam, Jack L Arbiser, Michael Y Bonner, Neeraj K Saxena, Dipali SharmaAbstract:Introduction Honokiol, a small-molecule polyphenol isolated from magnolia species, is widely known for its therapeutic potential as an antiinflammatory, antithrombosis, and Antioxidant Agent, and more recently, for its protective function in the pathogenesis of carcinogenesis. In the present study, we sought to examine the effectiveness of honokiol in inhibiting migration and invasion of breast cancer cells and to elucidate the underlying molecular mechanisms.
Etin Rohmatin - One of the best experts on this subject based on the ideXlab platform.
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indonesian honey protein isolation apis dorsata dorsata and tetragonula sp as antibacterial and Antioxidant Agent
2nd Biomedical Engineering's Recent Progress in Biomaterials Drugs Development and Medical Devices ISBE 2017, 2018Co-Authors: Muhamad Sahlan, Masafumi Yohda, Vina Damayanti, Nurul Azizah, Kazuaki Hakamada, Heri Hermansyah, Anondho Wijanarko, Etin RohmatinAbstract:Honey is a natural product that has many properties and been widely used for many theurapeutic purposes. Research on honey has been very rapid but not yet for Indonesia. Like local Indonesian honey Apis dorsata dorsata and Tetragonula sp. which has been widely consumed by the public but not yet known for certain efficacy of each content. The function of honey as antibacterial and Antioxidant has not been specifically explained by the components contained in honey. Protein is one of the content of honey that turned out to have activity as an antibacterial and Antioxidant in certain types of honey because of it antimicrobial peptide. Testing of honey activity as antibacterial and Antioxidant through several stages including isolation, SDS-PAGE analysis, Bradford test, antibacterial activity test with well diffusion method and Antioxidant activity test by DPPH method. Bacteria used were gram-positive bacteria Staphylococcus aureus and gram negative Escherichia coli. After some experiment finally got protein ...
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indonesian honey protein isolation apis dorsata dorsata and tetragonula sp as antibacterial and Antioxidant Agent
2nd Biomedical Engineering's Recent Progress in Biomaterials Drugs Development and Medical Devices ISBE 2017, 2018Co-Authors: Muhamad Sahlan, Masafumi Yohda, Vina Damayanti, Nurul Azizah, Kazuaki Hakamada, Heri Hermansyah, Anondho Wijanarko, Etin RohmatinAbstract:Honey is a natural product that has many properties and been widely used for many theurapeutic purposes. Research on honey has been very rapid but not yet for Indonesia. Like local Indonesian honey Apis dorsata dorsata and Tetragonula sp. which has been widely consumed by the public but not yet known for certain efficacy of each content. The function of honey as antibacterial and Antioxidant has not been specifically explained by the components contained in honey. Protein is one of the content of honey that turned out to have activity as an antibacterial and Antioxidant in certain types of honey because of it antimicrobial peptide. Testing of honey activity as antibacterial and Antioxidant through several stages including isolation, SDS-PAGE analysis, Bradford test, antibacterial activity test with well diffusion method and Antioxidant activity test by DPPH method. Bacteria used were gram-positive bacteria Staphylococcus aureus and gram negative Escherichia coli. After some experiment finally got protein isolation method that is in the form of further concentration using Millipore membrane for honey Tetragonula sp. and membrane filtration dot blot for honey Apis dorsata dorsata. The Bradford assay showed that Apis dorsata dorsata honey contains protein <5 µg / ml, while honey Tetragonula sp. has a protein content of 97 µg / ml. The characteristic profile of molecular weight of the protein showed honey Tetragonula sp. has 3 protein bands composed of 52, 96 – 61,9 kDa, 63,35 – 65,92 kDa and 86,16 – 91,4 kDa, whereas Apis dorsata dorsata honey has 5 protein bands consisting of 45,2 – 46,6 kDa, 50,2 – 50,9 kDa, 62,5 – 62,9 kDa, 73,1 – 73,9 kDa, 83,9 – 86,9 kDa. Isolate honey protein Apis dorsata dorsata has no Antioxidant and antibacterial activity (Staphylococcus aureus and Escherichia coli), whereas honey protein isolates Tetragonula sp. has antibacterial activity against Escherichia coli.
Ahmet Koyu - One of the best experts on this subject based on the ideXlab platform.
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a novel Antioxidant Agent caffeic acid phenethyl ester prevents long term mobile phone exposure induced renal impairment in rat
Molecular and Cellular Biochemistry, 2005Co-Authors: Fehmi Ozguner, Ahmet Koyu, Faruk Oktem, Ali Ayata, Ramazan H YilmazAbstract:Caffeic acid phenethyl ester (CAPE), a flavonoid like compound, is one of the major components of honeybee propolis. It has been used in folk medicine for many years in Middle East countries. It was found to be a potent free radical scavenger and Antioxidant recently. The aim of this study was to examine long-term applied 900 MHz emitting mobile phone-induced oxidative stress that promotes production of reactive oxygen species (ROS) and, was to investigate the role of CAPE on kidney tissue against the possible electromagnetic radiation (EMR)-induced renal impairment in rats. In particular, the ROS such as superoxide and nitric oxide (NO) may contribute to the pathophysiology of EMR-induced renal impairment. Malondialdehyde (MDA, an index of lipid peroxidation) levels, urinary N-acetyl-β-d-glucosaminidase (NAG, a marker of renal tubular injury) and nitric oxide (NO, an oxidant product) levels were used as markers of oxidative stress-induced renal impairment and the success of CAPE treatment. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in renal tissue were determined to evaluate the changes of Antioxidant status. The rats used in the study were randomly grouped (10 each) as follows: i) Control group (without stress and EMR), ii) Sham-operated rats stayed without exposure to EMR (exposure device off), iii) Rats exposed to 900 MHz EMR (EMR group), and iv) A 900 MHz EMR exposed + CAPE treated group (EMR + CAPE group). In the EMR exposed group, while tissue MDA, NO levels and urinary NAG levels increased (p < 0.0001), the activities of SOD, CAT, and GSH-Px in renal tissue were reduced (p < 0.001). CAPE treatment reversed these effects as well (p < 0.0001, p < 0.001 respectively). In conclusion, the increase in NO and MDA levels of renal tissue, and in urinary NAG with the decrease in renal SOD, CAT, GSH-Px activities demonstrate the role of oxidative mechanisms in 900 MHz mobile phone-induced renal tissue damage, and CAPE, via its free radical scavenging and Antioxidant properties, ameliorates oxidative renal damage. These results strongly suggest that CAPE exhibits a protective effect on mobile phone-induced and free radical mediated oxidative renal impairment in rats.
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a novel Antioxidant Agent caffeic acid phenethyl ester prevents shock wave induced renal tubular oxidative stress
Urological Research, 2005Co-Authors: Fehmi Ozguner, Abdullah Armagan, Ahmet Koyu, Sadettin Caliskan, Halis KoyluAbstract:The aim of this study was to evaluate the effects of the novel free radical scavenger caffeic acid phenethyl ester (CAPE) on extracorporeal shock wave lithotripsy (ESWL) induced renal impairment. The study was performed using 30 rabbits which were divided into two groups, each exposed to 3,000 shock waves at 18 kV: (1) control group, (2) ESWL+CAPE treated group. Malodialdehyde (MDA), urine N-acetyl-β-glucosaminidase (NAG) activity, uric acid and white cell counts were used as markers of oxidative stress. Following shock wave exposure there was a significant rise in MDA, NAG and uric acid and white cell counts. CAPE reduced the rise in MDA, NAG, uric acid and white cell counts. Thus CAPE treatment to a great extent prevented the induction of these renal changes. Our results suggest that the Antioxidant capacity of the kidney tissue was reduced after ESWL treatment and that the tissue was exposed to oxidant stress. We conclude that CAPE treatment provided significant protection against ESWL induced free radical damage.