The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform

Sherif A F Rostom - One of the best experts on this subject based on the ideXlab platform.

Lizeng Cheng - One of the best experts on this subject based on the ideXlab platform.

  • Antitumor activity of se containing tea polysaccharides against sarcoma 180 and comparison with regular tea polysaccharides and se yeast
    International Journal of Biological Macromolecules, 2018
    Co-Authors: Lizeng Cheng, Liang Chen, Qiongqiong Yang, Yuanfeng Wang, Xinlin Wei
    Abstract:

    Selenium (Se)-containing polysaccharide, a Se-conjugate macromolecule, generally exhibited higher Antitumor activity than its regular polysaccharide. Previously, we extracted Se-containing tea polysaccharides (Se-TPS) from Se-enriched tea, and explored its structure and antioxidant activity. In this study, we investigated Antitumor activity of Se-TPS on sarcoma 180 (S-180), and compared with its regular polysaccharides TPS and dietary supplement Se-yeast. In vitro Antitumor activity of Se-TPS was evaluated by MTT and LDH assays, and the results indicated that Se-TPS can significantly inhibit the proliferation of S-180 in dose-dependent manner (R2=0.97, p<0.0001). In S-180 cancer xenograft model in Kunming mice, Se-TPS oral administration at three doses of 50, 100 and 200mg/kg body weight daily for 13days resulted in significant tumor regression. At the same dose, Se-TPS exhibited significantly higher Antitumor activity than TPS and Se-yeast. Importantly, Se-TPS can significantly increase the spleen and thymus indices of tumor-bearing mice, suggesting the safety and immunomodulatory activity of Se-TPS. Therefore, Se-TPS may be a desirable Antitumor agent for therapeutic and immunomodulatory applications.

Xinlin Wei - One of the best experts on this subject based on the ideXlab platform.

  • Antitumor activity of se containing tea polysaccharides against sarcoma 180 and comparison with regular tea polysaccharides and se yeast
    International Journal of Biological Macromolecules, 2018
    Co-Authors: Lizeng Cheng, Liang Chen, Qiongqiong Yang, Yuanfeng Wang, Xinlin Wei
    Abstract:

    Selenium (Se)-containing polysaccharide, a Se-conjugate macromolecule, generally exhibited higher Antitumor activity than its regular polysaccharide. Previously, we extracted Se-containing tea polysaccharides (Se-TPS) from Se-enriched tea, and explored its structure and antioxidant activity. In this study, we investigated Antitumor activity of Se-TPS on sarcoma 180 (S-180), and compared with its regular polysaccharides TPS and dietary supplement Se-yeast. In vitro Antitumor activity of Se-TPS was evaluated by MTT and LDH assays, and the results indicated that Se-TPS can significantly inhibit the proliferation of S-180 in dose-dependent manner (R2=0.97, p<0.0001). In S-180 cancer xenograft model in Kunming mice, Se-TPS oral administration at three doses of 50, 100 and 200mg/kg body weight daily for 13days resulted in significant tumor regression. At the same dose, Se-TPS exhibited significantly higher Antitumor activity than TPS and Se-yeast. Importantly, Se-TPS can significantly increase the spleen and thymus indices of tumor-bearing mice, suggesting the safety and immunomodulatory activity of Se-TPS. Therefore, Se-TPS may be a desirable Antitumor agent for therapeutic and immunomodulatory applications.

Thomas E Wagner - One of the best experts on this subject based on the ideXlab platform.

  • whole tumor cell vaccine with irradiated s180 cells as adjuvant
    Vaccine, 2009
    Co-Authors: Jinhua Li, Ada V King, Sara L Stickel, Kelly E Burgin, Thomas E Wagner, Xinhai Zhang
    Abstract:

    Whole tumor cell vaccines have been widely studied and remain promising cancer immunotherapies. In the present study, we discovered that vaccination with irradiated mouse sarcoma S180 tumor cells stimulated robust Antitumor immunity to autologous tumor cells in both syngenic and allogenic mice. The Antitumor activity requires both T and B cells, but not NK cells. When a mouse lung carcinoma (TC-1) whole tumor cell vaccine was combined with the S180 vaccine, the Antitumor immunity against live TC-1 tumor cells is significantly enhanced compared to a TC-1 whole cell vaccine alone. This Antitumor immunity not only prevents live tumor challenge but also eradicates existing tumor cells. A similar phenomenon was also observed when S180 vaccine was combined with LL2 Lewis lung carcinoma tumor cells. Therefore, S180 vaccine may serve as an adjuvant for other whole tumor cell vaccines.

Hideaki Tahara - One of the best experts on this subject based on the ideXlab platform.

  • therapeutic and specific Antitumor immunity induced by co administration of immature dendritic cells and adenoviral vector expressing biologically active il 18
    Gene Therapy, 2002
    Co-Authors: Michael T Lotze, Fujie Tanaka, Wataru Hashimoto, Paul D Robbins, Hideaki Tahara
    Abstract:

    Interleukin-18 is a potent cytokine expressed early in the immune response following cleavage in activated composes. We have investigated the in vivo Antitumor effects of intratumoral (i.t.) administration of an adenoviral vector expressing biologically active murine interleukin (IL)-18 (Ad.PTH.IL-18). Substantial Antitumor effects were observed when established MCA205 fibrosarcoma was treated in syngeneic immunocompetent mice with intratumoral injection of Ad.PTH.IL-18 (P = 0.0025 versus control vector treatment), generating potent cytotoxic T lymphocytes (CTLs) in culture. In contrast, the Antitumor effect was absent, and cytotoxic activity was significantly less (P = 0.021) in gld mice (Fas ligand deficient). To enhance the in vivo Antitumor activity of the treatment using Ad.PTH.IL-18, we co-injected immature DC and Ad.PTH.IL-18 i.t. into established, day 7 MCA205 fibrosarcoma and MC38 adenocarcinoma. Co-injection of both Ad.PTH.IL-18 and DC was associated with complete abrogation of injected tumors. Furthermore, the Antitumor effects were also observed on distant tumors inoculated i.d. in the contralateral flank of the animal. The induced cytolytic activity was tumor-specific and MHC class I-restricted. As we have previously demonstrated in vitro (Tanaka F et al, Cancer Res 2000; 60: 4838–4844) and consistent with these findings in vivo, NK, T and dendritic cells coactivately mediate the IL-18 enhanced Antitumor effect. This study suggests that the coactivate strategy could be used in the clinical setting to treat patients with cancer.