The Experts below are selected from a list of 279 Experts worldwide ranked by ideXlab platform
Jaroslav G. Vostal - One of the best experts on this subject based on the ideXlab platform.
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Neutrophil depletion reduced platelet sequestration in the lungs.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:SCID mouse were pretreated with either Gr-1 mAb (250 ug, i.p.) or isotype control (Iso Ctrl, i.p.) 24 hours before LPS administration (3 mg/kg, i.v.), followed by transfusion of control hPLTs or UVB-treated hPLTs. A: Confocal images of lung sections from SCID mice stained with anti-CD41antibodies (red). Blue fluorescence represents Hoechst33342 stained nuclei. The composite images shown were representative of 6 independent experiments. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. B: Quantification of fluorescence intensity of anti-CD41 antibody staining on mouse lung sections. Data are expressed as means ± SD; n = 6 .* p
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Treatment with aspirin in vitro prior to UV exposure abolished UVB-hPLT sequestration in the lung.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:A: Confocal images of SCID mouse lung sections. Pre-treatment with aspirin in vitro abolished UVB-induced hPLT sequestration in the lung (A-c), compared with vehicle control (A-d). All sections were stained with anti-hCD41 antibodies (red). Blue fluorescence represents TO-PRO-3 stained nuclei. The composite images shown were representative of 3 independent experiments. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. B: Quantification of fluorescence intensity of anti-hCD41 antibody staining on mouse lung sections shown in A, n = 3. C: Aspirin treatment in vitro inhibited hPLTs aggregation induced by TRAP (20 µM). Maximum Aggregation (MA) was shown in solid black bar and Dis-Aggregation (DA) was shown in solid grey bar, n = 4. All data are expressed as means ± SD, ** p
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Infusion of UVB-treated hPLTs resulted in retention of both mouse and human PLTs in the lungs of LPS primed SCID mice.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:A: Confocal images of mouse lung sections. More mouse PLTs were sequestered in the lungs of SCID; CD41-YFP mice after UVB-treated human platelet injection (A-b,d), compared with the control (A-a, c), visualized by endogenous CD41-YFP fluorescence on fresh frozen sections (A-a, b) or anti-YFP antibody staining on fixed frozen sections (A-c, d). More hPLTs were sequestered in the lungs of the mice after UVB-treated hPLT injection (A-f), compared with the control (A-e), visualized by anti-hCD41 antibody staining on fixed frozen sections. The composite images shown were representative of 5 independent experiments. B: Quantification of pixel intensity of endogenous YFP fluorescence on SCID; CD41-YFP mouse lung sections. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. Data are expressed as means ± SD; n = 5. **p
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UVB treated hPLTs sequestered in the lungs of LPS primed SCID mice whereas TRAP (4 µM) activated hPLTs did not.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:A: Confocal images of lung sections of healthy (a to d) and septic (e to h) SCID mice infused with control human platelets (Ctrl PLT), UVB-treated human platelets (UVB PLT) or TRAP (4 uM) activated human platelets (Representative images from five independent experiments). In healthy mice, there were few hPLTs in the lung in any treatment group (a to d). Sequestration of significant number of hPLTs in the lung of LPS primed mice infused with UVB treated hPLTs was observed (g), whereas a much lower numbers of Ctrl PLT (f) or TRAP-activated hPLTs (h) were sequestered in the lung. Sections were stained with anti-hCD41 antibodies (red). Blue fluorescence represents Hoechst33342 stained nuclei. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. B: Quantification of the fluorescence intensity of anti-hCD41 antibody staining on mouse lung sections. Data are expressed as means ± SD; n = 5. ** p
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Mouse PLT depletion abolished hPLT sequestration in the lungs of LPS primed SCID mice.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:A: Confocal images of mouse lung or liver sections. GPIb antibody treatment abolished hPLT sequestration in the lungs of septic SCID mice (A-c,d vs. a, b), visualized by anti-hCD41 antibody staining. Yet GPIb antibody treatment did not abolish platelet accumulation in the liver (A-g, h vs e, f). Neutrophil count, visualized by endogenous LYS-eGFP fluorescence, in the lung was similar before and after PLT depletion (A-k vs i, l vs j). The composite images shown were representative of 5 independent experiments. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. B: Quantification of fluorescence pixel intensity of anti-hCD41 antibody staining on SCID; CD41-YFP mouse lung sections shown in A-a to d. C: Quantification of fluorescence pixel intensity of anti-hCD41 antibody staining on SCID; CD41-YFP mouse liver sections shown in A-e to h. Data are expressed as means ± SD; n = 5. *p
Jonathan Maxwell - One of the best experts on this subject based on the ideXlab platform.
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Tertiary-spectrum manipulation in Apochromats.
Applied optics, 1992Co-Authors: Jonathan MaxwellAbstract:By combining achromatic and superachromatic lens groups we may achieve improved tertiary-spectrum residuals in Apochromatic systems. The application of this technique is reported. The significance of a secondary spectrum and a tertiary spectrum in lens design is also reviewed.
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Tertiary spectrum manipulation in Apochromats
1990 Intl Lens Design Conf, 1991Co-Authors: Jonathan MaxwellAbstract:By combining achromatic and super achromatic lens groups we may achieve improved tertiary spectrum residuals in Apochromatic systems. The application of this technique is reported. 1.
Xuan Chi - One of the best experts on this subject based on the ideXlab platform.
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Neutrophil depletion reduced platelet sequestration in the lungs.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:SCID mouse were pretreated with either Gr-1 mAb (250 ug, i.p.) or isotype control (Iso Ctrl, i.p.) 24 hours before LPS administration (3 mg/kg, i.v.), followed by transfusion of control hPLTs or UVB-treated hPLTs. A: Confocal images of lung sections from SCID mice stained with anti-CD41antibodies (red). Blue fluorescence represents Hoechst33342 stained nuclei. The composite images shown were representative of 6 independent experiments. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. B: Quantification of fluorescence intensity of anti-CD41 antibody staining on mouse lung sections. Data are expressed as means ± SD; n = 6 .* p
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Treatment with aspirin in vitro prior to UV exposure abolished UVB-hPLT sequestration in the lung.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:A: Confocal images of SCID mouse lung sections. Pre-treatment with aspirin in vitro abolished UVB-induced hPLT sequestration in the lung (A-c), compared with vehicle control (A-d). All sections were stained with anti-hCD41 antibodies (red). Blue fluorescence represents TO-PRO-3 stained nuclei. The composite images shown were representative of 3 independent experiments. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. B: Quantification of fluorescence intensity of anti-hCD41 antibody staining on mouse lung sections shown in A, n = 3. C: Aspirin treatment in vitro inhibited hPLTs aggregation induced by TRAP (20 µM). Maximum Aggregation (MA) was shown in solid black bar and Dis-Aggregation (DA) was shown in solid grey bar, n = 4. All data are expressed as means ± SD, ** p
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Infusion of UVB-treated hPLTs resulted in retention of both mouse and human PLTs in the lungs of LPS primed SCID mice.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:A: Confocal images of mouse lung sections. More mouse PLTs were sequestered in the lungs of SCID; CD41-YFP mice after UVB-treated human platelet injection (A-b,d), compared with the control (A-a, c), visualized by endogenous CD41-YFP fluorescence on fresh frozen sections (A-a, b) or anti-YFP antibody staining on fixed frozen sections (A-c, d). More hPLTs were sequestered in the lungs of the mice after UVB-treated hPLT injection (A-f), compared with the control (A-e), visualized by anti-hCD41 antibody staining on fixed frozen sections. The composite images shown were representative of 5 independent experiments. B: Quantification of pixel intensity of endogenous YFP fluorescence on SCID; CD41-YFP mouse lung sections. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. Data are expressed as means ± SD; n = 5. **p
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UVB treated hPLTs sequestered in the lungs of LPS primed SCID mice whereas TRAP (4 µM) activated hPLTs did not.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:A: Confocal images of lung sections of healthy (a to d) and septic (e to h) SCID mice infused with control human platelets (Ctrl PLT), UVB-treated human platelets (UVB PLT) or TRAP (4 uM) activated human platelets (Representative images from five independent experiments). In healthy mice, there were few hPLTs in the lung in any treatment group (a to d). Sequestration of significant number of hPLTs in the lung of LPS primed mice infused with UVB treated hPLTs was observed (g), whereas a much lower numbers of Ctrl PLT (f) or TRAP-activated hPLTs (h) were sequestered in the lung. Sections were stained with anti-hCD41 antibodies (red). Blue fluorescence represents Hoechst33342 stained nuclei. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. B: Quantification of the fluorescence intensity of anti-hCD41 antibody staining on mouse lung sections. Data are expressed as means ± SD; n = 5. ** p
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Mouse PLT depletion abolished hPLT sequestration in the lungs of LPS primed SCID mice.
2012Co-Authors: Xuan Chi, Li Zhi, Monique P. Gelderman, Jaroslav G. VostalAbstract:A: Confocal images of mouse lung or liver sections. GPIb antibody treatment abolished hPLT sequestration in the lungs of septic SCID mice (A-c,d vs. a, b), visualized by anti-hCD41 antibody staining. Yet GPIb antibody treatment did not abolish platelet accumulation in the liver (A-g, h vs e, f). Neutrophil count, visualized by endogenous LYS-eGFP fluorescence, in the lung was similar before and after PLT depletion (A-k vs i, l vs j). The composite images shown were representative of 5 independent experiments. All images were taken using a Zeiss 710 laser scanning confocal microscope, with a 63×/NA1.4 Plan-Apochromat oil objective. B: Quantification of fluorescence pixel intensity of anti-hCD41 antibody staining on SCID; CD41-YFP mouse lung sections shown in A-a to d. C: Quantification of fluorescence pixel intensity of anti-hCD41 antibody staining on SCID; CD41-YFP mouse liver sections shown in A-e to h. Data are expressed as means ± SD; n = 5. *p
Lyle R. Middendorf - One of the best experts on this subject based on the ideXlab platform.
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Imaging of single-chromophore molecules in aqueous solution near a fused-silica interface
2014Co-Authors: Lloyd M. Davis, Wesley C. Parker, David A. Ball, John G. K. Williams, Greg R. Bashford, Pamela Sheaff, Robert Eckles, Don T. Lamb, Lyle R. MiddendorfAbstract:Single molecules of unconjugated Bodipy-Texas Red (BTR), BTR-dimer, and BTR conjugated to cysteine, in aqueous solutions are imaged using total-internal-reflection excitation and through-sample collection of fluorescence onto an intensified CCD camera, or a back-illuminated frame transfer CCD. The sample excitation is provided by the beam from a continuous-wave krypton ion laser, or a synchronously-pumped dye laser, operating at 568 nm. In order to essentially freeze molecular motion due to diffusion and thereby enhance image contrast, the laser beam is first passed through a mechanical shutter, which yields a 3-millisecond laser exposure for each camera frame. The laser beam strikes the fused-silica/sample interface at an angle exceeding the critical angle by about 1 degree. The resultant evanescent wave penetrates into the sample a depth of approximately 0.3 microns. Fluorescence from the thin plane of illumination is then imaged onto the camera by a water immersion Apochromat (NA 1.2, WD 0.2 mm). A Raman notch filter blocks Rayleigh and specular laser scatter and a band-pass-filter blocks most Raman light scatter that originates from the solvent. Single molecules that have diffused into the evanescent zone at the time of laser exposure yield near-diffraction-limited Airy disk images with diameters of ~5 pixels. While most molecules diffuse out of the evanescent zone before the next laser exposure, stationary or slowly moving molecules persisting over several frames, and blinking of such molecules, are occasionally observed
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Imaging of single-chromophore molecules in aqueous solution near a fused-silica interface
Multiphoton Microscopy in the Biomedical Sciences, 2001Co-Authors: Lloyd M. Davis, Wesley C. Parker, David A. Ball, John G. K. Williams, Greg R. Bashford, Pamela Sheaff, Robert Eckles, Don T. Lamb, Lyle R. MiddendorfAbstract:Single molecules of unconjugated Bodipy-Texas Red (BTR), BTR-dimer, and BTR conjugated to cysteine, in aqueous solutions are imaged using total-internal-reflection excitation and through-sample collection of fluorescence onto an intensified CCD camera, or a back-illuminated frame transfer CCD. The sample excitation is provided by the beam from a continuous-wave krypton ion laser, or a synchronously-pumped dye laser, operating at 568 nm. In order to essentially freeze molecular motion due to diffusion and thereby enhance image contrast, the laser beam is first passed through a mechanical shutter, which yields a 3-millisecond laser exposure for each camera frame. The laser beam strikes the fused-silica/sample interface at an angle exceeding the critical angle by about 1 degree. The resultant evanescent wave penetrates into the sample a depth of approximately 0.3 microns. Fluorescence from the thin plane of illumination is then imaged onto the camera by a water immersion Apochromat (NA 1.2, WD 0.2mm). A Raman notch filter blocks Rayleigh and specular laser scatter and a band-pass-filter blocks most Raman light scatter that originates from the solvent. Single molecules that have diffused into the evanescent zone at the time of laser exposure yield near-diffraction-limited Airy disk images with diameters of ~5 pixels. While most molecules diffuse out of the evanescent zone before the next laser exposure, stationary or slowly moving molecules persisting over several frames, and blinking of such molecules are occasionally observed.© (2001) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Gao Weichuan - One of the best experts on this subject based on the ideXlab platform.
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Selected Topics in Advanced Optical Design and Engineering
The University of Arizona., 2018Co-Authors: Gao WeichuanAbstract:Optical imaging has seen significant development over the last few decades thanks to the advances of light sources and innovative imaging modalities. New quests have opened for advanced optical system design and engineering that require comprehensive understanding of theory and extensive computer simulations. This dissertation discusses several selected topics in advanced optical design and engineering, including new optical design considerations for systems with ultrafast illumination, engineering of a reflective microscope system in the vacuum ultraviolet and a method to design Apochromat and superachromat objectives. For optical systems with ultrafast illumination, a modified definition of Strehl ratio is proposed to quantify chromatic and temporal behavior of ultrafast laser pulses at the optical focus. A simple method to obtain approximate numerical solutions is given with the help of ray tracing software. Effects of monochromatic aberrations, material dispersion up to the second order and pupil aberrations are discussed. System engineering of an imaging microscope illuminated with hydrogen Lyman-α line at 121.6nm is discussed. Challenges of diamond turning fabrication technology are described. Alignment and testing procedures are presented. A correction phase plate design is proposed for improving the as-built system performance, and a sensitivity analysis is carried out. For future work, a new two-stage system design is proposed to address the limitations of the current system. A simple method to design Apochromats is proposed, where an Apochromat is formed by combining two achromatic doublets with proper scaling of the focal lengths. A scaling formula is derived to calculate the focal lengths of each component. The formula is developed for lenses in contact and remote lenses with different marginal ray heights.Release after 01/27/201
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Selected Topics in Advanced Optical Design and Engineering
The University of Arizona, 2018Co-Authors: Gao WeichuanAbstract:Optical imaging has seen significant development over the last few decades thanks to the advances of light sources and innovative imaging modalities. New quests have opened for advanced optical system design and engineering that require comprehensive understanding of theory and extensive computer simulations. This dissertation discusses several selected topics in advanced optical design and engineering, including new optical design considerations for systems with ultrafast illumination, engineering of a reflective microscope system in the vacuum ultraviolet and a method to design Apochromat and superachromat objectives. For optical systems with ultrafast illumination, a modified definition of Strehl ratio is proposed to quantify chromatic and temporal behavior of ultrafast laser pulses at the optical focus. A simple method to obtain approximate numerical solutions is given with the help of ray tracing software. Effects of monochromatic aberrations, material dispersion up to the second order and pupil aberrations are discussed. System engineering of an imaging microscope illuminated with hydrogen Lyman-? line at 121.6nm is discussed. Challenges of diamond turning fabrication technology are described. Alignment and testing procedures are presented. A correction phase plate design is proposed for improving the as-built system performance, and a sensitivity analysis is carried out. For future work, a new two-stage system design is proposed to address the limitations of the current system. A simple method to design Apochromats is proposed, where an Apochromat is formed by combining two achromatic doublets with proper scaling of the focal lengths. A scaling formula is derived to calculate the focal lengths of each component. The formula is developed for lenses in contact and remote lenses with different marginal ray heights