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R A Anderson - One of the best experts on this subject based on the ideXlab platform.
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distribution of lipids between the zooxanthellae and animal compartment in the symbiotic sea anemoneanemonia viridis wax esters triglycerides and fatty acids
Marine Biology, 1991Co-Authors: A D Harland, L M Fixter, Spencer P Davies, R A AndersonAbstract:The temperate sea anemoneAnemonia viridis (Forskal) contained about 11% lipid on a dry weight basis when maintained at light levels of about 10µE m−2 s−1 and a temperature of 10°C. Aposymbiotic forms of the anemone had similar lipid levels. These values are very low compared with tropical symbiotic Anthozoa in which lipid levels constitute up to 50% of dry weight. In symbioticA. viridis, <6% of total lipid consisted of the storage lipids, wax esters and triglycerides. Most of the triglyceride was stored in the animal tissues rather than the zooxanthellae. Zooxanthellae contained only small amounts of wax esters. An analysis was made of the wax ester, triglyceride and fatty acid composition of symbiotic anemones, isolated zooxanthellae and Aposymbiotic anemones. Wax ester composition was similar in symbiotic and Aposymbiotic forms. However, triglyceride composition differed. In particular trimyristin (C42) was found only within the symbiotic association. Fatty acids showed a high degree of unsaturation, and acids with both even and odd numbers of carbon atoms were found. The most abundant fatty acid was 16:0 in all samples, except for the total lipids from zooxanthellae in which the major fatty acid wastrans-18:1.
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Distribution of lipids between the zooxanthellae and animal compartment in the symbiotic sea anemoneAnemonia viridis: Wax esters, triglycerides and fatty acids
Marine Biology, 1991Co-Authors: A D Harland, L M Fixter, P. Spencer Davies, R A AndersonAbstract:The temperate sea anemone Anemonia viridis (Forskäl) contained about 11% lipid on a dry weight basis when maintained at light levels of about 10 µ E m^−2 s^−1 and a temperature of 10°C. Aposymbiotic forms of the anemone had similar lipid levels. These values are very low compared with tropical symbiotic Anthozoa in which lipid levels constitute up to 50% of dry weight. In symbiotic A. viridis ,
A D Harland - One of the best experts on this subject based on the ideXlab platform.
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distribution of lipids between the zooxanthellae and animal compartment in the symbiotic sea anemoneanemonia viridis wax esters triglycerides and fatty acids
Marine Biology, 1991Co-Authors: A D Harland, L M Fixter, Spencer P Davies, R A AndersonAbstract:The temperate sea anemoneAnemonia viridis (Forskal) contained about 11% lipid on a dry weight basis when maintained at light levels of about 10µE m−2 s−1 and a temperature of 10°C. Aposymbiotic forms of the anemone had similar lipid levels. These values are very low compared with tropical symbiotic Anthozoa in which lipid levels constitute up to 50% of dry weight. In symbioticA. viridis, <6% of total lipid consisted of the storage lipids, wax esters and triglycerides. Most of the triglyceride was stored in the animal tissues rather than the zooxanthellae. Zooxanthellae contained only small amounts of wax esters. An analysis was made of the wax ester, triglyceride and fatty acid composition of symbiotic anemones, isolated zooxanthellae and Aposymbiotic anemones. Wax ester composition was similar in symbiotic and Aposymbiotic forms. However, triglyceride composition differed. In particular trimyristin (C42) was found only within the symbiotic association. Fatty acids showed a high degree of unsaturation, and acids with both even and odd numbers of carbon atoms were found. The most abundant fatty acid was 16:0 in all samples, except for the total lipids from zooxanthellae in which the major fatty acid wastrans-18:1.
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Distribution of lipids between the zooxanthellae and animal compartment in the symbiotic sea anemoneAnemonia viridis: Wax esters, triglycerides and fatty acids
Marine Biology, 1991Co-Authors: A D Harland, L M Fixter, P. Spencer Davies, R A AndersonAbstract:The temperate sea anemone Anemonia viridis (Forskäl) contained about 11% lipid on a dry weight basis when maintained at light levels of about 10 µ E m^−2 s^−1 and a temperature of 10°C. Aposymbiotic forms of the anemone had similar lipid levels. These values are very low compared with tropical symbiotic Anthozoa in which lipid levels constitute up to 50% of dry weight. In symbiotic A. viridis ,
L M Fixter - One of the best experts on this subject based on the ideXlab platform.
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distribution of lipids between the zooxanthellae and animal compartment in the symbiotic sea anemoneanemonia viridis wax esters triglycerides and fatty acids
Marine Biology, 1991Co-Authors: A D Harland, L M Fixter, Spencer P Davies, R A AndersonAbstract:The temperate sea anemoneAnemonia viridis (Forskal) contained about 11% lipid on a dry weight basis when maintained at light levels of about 10µE m−2 s−1 and a temperature of 10°C. Aposymbiotic forms of the anemone had similar lipid levels. These values are very low compared with tropical symbiotic Anthozoa in which lipid levels constitute up to 50% of dry weight. In symbioticA. viridis, <6% of total lipid consisted of the storage lipids, wax esters and triglycerides. Most of the triglyceride was stored in the animal tissues rather than the zooxanthellae. Zooxanthellae contained only small amounts of wax esters. An analysis was made of the wax ester, triglyceride and fatty acid composition of symbiotic anemones, isolated zooxanthellae and Aposymbiotic anemones. Wax ester composition was similar in symbiotic and Aposymbiotic forms. However, triglyceride composition differed. In particular trimyristin (C42) was found only within the symbiotic association. Fatty acids showed a high degree of unsaturation, and acids with both even and odd numbers of carbon atoms were found. The most abundant fatty acid was 16:0 in all samples, except for the total lipids from zooxanthellae in which the major fatty acid wastrans-18:1.
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Distribution of lipids between the zooxanthellae and animal compartment in the symbiotic sea anemoneAnemonia viridis: Wax esters, triglycerides and fatty acids
Marine Biology, 1991Co-Authors: A D Harland, L M Fixter, P. Spencer Davies, R A AndersonAbstract:The temperate sea anemone Anemonia viridis (Forskäl) contained about 11% lipid on a dry weight basis when maintained at light levels of about 10 µ E m^−2 s^−1 and a temperature of 10°C. Aposymbiotic forms of the anemone had similar lipid levels. These values are very low compared with tropical symbiotic Anthozoa in which lipid levels constitute up to 50% of dry weight. In symbiotic A. viridis ,
Spencer P Davies - One of the best experts on this subject based on the ideXlab platform.
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distribution of lipids between the zooxanthellae and animal compartment in the symbiotic sea anemoneanemonia viridis wax esters triglycerides and fatty acids
Marine Biology, 1991Co-Authors: A D Harland, L M Fixter, Spencer P Davies, R A AndersonAbstract:The temperate sea anemoneAnemonia viridis (Forskal) contained about 11% lipid on a dry weight basis when maintained at light levels of about 10µE m−2 s−1 and a temperature of 10°C. Aposymbiotic forms of the anemone had similar lipid levels. These values are very low compared with tropical symbiotic Anthozoa in which lipid levels constitute up to 50% of dry weight. In symbioticA. viridis, <6% of total lipid consisted of the storage lipids, wax esters and triglycerides. Most of the triglyceride was stored in the animal tissues rather than the zooxanthellae. Zooxanthellae contained only small amounts of wax esters. An analysis was made of the wax ester, triglyceride and fatty acid composition of symbiotic anemones, isolated zooxanthellae and Aposymbiotic anemones. Wax ester composition was similar in symbiotic and Aposymbiotic forms. However, triglyceride composition differed. In particular trimyristin (C42) was found only within the symbiotic association. Fatty acids showed a high degree of unsaturation, and acids with both even and odd numbers of carbon atoms were found. The most abundant fatty acid was 16:0 in all samples, except for the total lipids from zooxanthellae in which the major fatty acid wastrans-18:1.
Fabrice Vavre - One of the best experts on this subject based on the ideXlab platform.
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Influence of Wolbachiaon host gene expression in an obligatory symbiosis
BMC Microbiology, 2012Co-Authors: Natacha Kremer, Delphine Charif, Hélène Henri, Frédérick Gavory, Patrick Wincker, Patrick Mavingui, Fabrice VavreAbstract:Background Wolbachia are intracellular bacteria known to be facultative reproductive parasites of numerous arthropod hosts. Apart from these reproductive manipulations, recent findings indicate that Wolbachia may also modify the host’s physiology, notably its immune function. In the parasitoid wasp, Asobara tabida , Wolbachia is necessary for oogenesis completion, and Aposymbiotic females are unable to produce viable offspring. The absence of egg production is also associated with an increase in programmed cell death in the ovaries of Aposymbiotic females, suggesting that a mechanism that ensures the maintenance of Wolbachia in the wasp could also be responsible for this dependence. In order to decipher the general mechanisms underlying host- Wolbachia interactions and the origin of the dependence, we developed transcriptomic approaches to compare gene expression in symbiotic and Aposymbiotic individuals. Results As no genetic data were available on A. tabida , we constructed several Expressed Sequence Tags (EST) libraries, and obtained 12,551 unigenes from this species. Gene expression was compared between symbiotic and Aposymbiotic ovaries through in silico analysis and in vitro subtraction (SSH). As pleiotropic functions involved in immunity and development could play a major role in the establishment of dependence, the expression of genes involved in oogenesis, programmed cell death (PCD) and immunity (broad sense) was analyzed by quantitative RT-PCR. We showed that Wolbachia might interfere with these numerous biological processes, in particular some related to oxidative stress regulation. We also showed that Wolbachia may interact with immune gene expression to ensure its persistence within the host. Conclusions This study allowed us to constitute the first major dataset of the transcriptome of A. tabida , a species that is a model system for both host/ Wolbachia and host/parasitoid interactions. More specifically, our results highlighted that symbiont infection may interfere with numerous pivotal processes at the individual level, suggesting that the impact of Wolbachia should also be investigated beyond reproductive manipulations.
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Do variable compensatory mechanisms explain the polymorphism of the dependence phenotype in the Asobara tabida-wolbachia association?
Evolution - International Journal of Organic Evolution, 2010Co-Authors: Natacha Kremer, Franck Dedeine, Delphine Charif, Cédric Finet, Roland Allemand, Fabrice VavreAbstract:Wolbachia are symbiotic intracellular bacteria, which are classified as reproductive parasites. Although generally facultative, Wolbachia is necessary for Asobara tabida (Hymenoptera), because Aposymbiotic females do not produce any offspring. Interestingly, the ovarian phenotype of Aposymbiotic females is variable: some females do not produce any eggs, whereas others do produce some eggs, but these are aborted. Here, we show that the ovarian phenotype of Aposymbiotic females is highly polymorphic within populations, although dependence remains complete in both cases. We also identified some lines in which Aposymbiotic females were able to produce a very few viable offspring, further extending the range of variation observed. These results suggest that various factors actively maintain polymorphism. We demonstrated that Wolbachia is necessary to trigger oogenetic processes, but that the ovarian phenotype was determined by the host only. Phenotypic variation was also correlated with the differential expression of genes controlling iron homeostasis and oxidative stress, which are potentially involved in the evolution of dependence. This suggests that variation in the ovarian phenotype could reflect selection for different levels of compensatory mechanisms in response to Wolbachia infection, and that polymorphism is maintained through selection on different antagonist traits influenced by oxidative stress.