The Experts below are selected from a list of 10260 Experts worldwide ranked by ideXlab platform
Xia Sun - One of the best experts on this subject based on the ideXlab platform.
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Novel electrochemical Aptasensor with dual signal amplification strategy for detection of acetamiprid.
The Science of the total environment, 2019Co-Authors: Shi Xiaojie, Yemin Guo, Sun Jianfei, Yao Yao, Huimin Liu, Jingcheng Huang, Xia SunAbstract:Abstract In this work, a novel dual signal amplification strategy for Aptasensor employing reduced graphene with silver nanoparticles and prussian blue-gold nanocomposites was developed for detection of acetamiprid. To improve the sensitivity of Aptasensors, reduced graphene oxide-silver nanoparticles (rGo-AgNPs) were modified on a bare glassy carbon electrode surface, which provided a large specific surface area for subsequent material immobilization and amplified current signal. The electrical signal output and sensitivity of the Aptasensor was significantly improved after the immobilization of prussian blue-gold nanoparticles (PB-AuNPs) as a catalyst for the redox reaction. The analysis experiment exhibited that it had super-high sensitivity with a detection limit of 0.30 pM (S/N = 3), which met the requirements of the vast majority of daily leaf vegetable testing. Under optimized conditions, the proposed Aptasensor showed a wide linear detection range from 1 pM to 1 μM. This Aptasensor also had good stability and high selectivity for acetamiprid detection without an interfering effect of some other pesticides. The proposed Aptasensor displayed good recovery rates in real samples, which proposed a new method for constructing electrochemical sensors and provided a novel tool for rapid, sensitive analysis of pesticides with low cost.
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a dual signal amplification strategy for kanamycin based on ordered mesoporous carbon chitosan gold nanoparticles streptavidin and ferrocene labelled dna
Analytica Chimica Acta, 2018Co-Authors: Yemin Guo, Xia Sun, Xiangyou Wang, Wenping ZhaoAbstract:Abstract An ultrasensitive electrochemical Aptasensor for kanamycin (KAN) detection was constructed with a dual-signal amplification strategy. The Aptasensor achieved greatly amplified sensitivity due to the excellent electrical conductivity of the ordered mesoporous carbon-chitosan (OMC-CS)/gold nanoparticles-streptavidin (AuNPs-SA) and DNA2 labelled with ferrocene (Fc-DNA2). The AuNPs-SA was used to immobilize the DNA strand (biotin labelled) with the biotin-streptavidin system. The DNA2 strand containing the KAN aptamer was labelled with ferrocene to increase the current signal on the electrode surface when bound to KAN. Some factors that affect the performance of the Aptasensor were optimized, and the proposed Aptasensor provided a wide linear range from 1 × 10−10 M to 4 × 10−6 M, with a detection limit as low as 35.69 pM for KAN under the optimized conditions. This Aptasensor had satisfactory electrochemical performance with good stability, sensitivity and reproducibility. Additionally, it also displayed a good specificity for KAN without interference from competitive analogues. Furthermore, the constructed Aptasensor was successfully used to detect KAN in a real milk sample. The proposed method for KAN detection has great potential for the detection of other antibiotics.
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Ratiometric electrochemical Aptasensor based on ferrocene and carbon nanofibers for highly specific detection of tetracycline residues.
Scientific reports, 2017Co-Authors: Xu Qingcui, Zengning Liu, Zhao Wenping, Yemin Guo, Xia Sun, Haiyun ZhangAbstract:A sensitive and efficient ratiometric electrochemical Aptasensor was designed for tetracycline (TET) detection in milk. The ratiometric electrochemical Aptasensor was constructed by integrating two Aptasensors termed as Aptasensor 1 and Aptasensor 2. The Aptasensor 1 was fabricated that based on ferrocene (Fc) and gold nanoparticles (AuNPs) nanocomposite. Meanwhile, the Aptasensor 2 was prepared that based on carbon nanofibers (CNFs) and AuNPs nanocomposite. TET-aptamer was immobilized effectively onto screen-printed carbon electrodes (SPCEs) surface through forming Au-S bond between AuNPs and thiol of aptamer at 5′ end to construct the Aptasensor 1 and Aptasensor 2. And their detection results were calculated by ratio. Thus, the proposed ratiometric Aptasensor solved the problem of low accuracy and large differences between batches. Under the optimized conditions, the TET was detected by differential pulse voltammetry (DPV). Taken advantage of ratio calculation, the as-prepared ratiometric Aptasensor could detect TET quantitatively in the range of 10−8–10−3gL−1, with a detection limit of 3.3 × 10−7gL−1. Moreover, its applicability to TET-contaminated real samples (milk) showed an excellent agreement with the values determined by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-ESI-MS/MS). With high sensitivity, accuracy and reliability, the developed ratiometric Aptasensor held a great potential in TET detection for food safety.
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An Aptasensor with dsDNA for rapid and highly sensitive detection of kanamycin in milk
RSC Advances, 2017Co-Authors: Li Falan, Xia Sun, Xiangyou Wang, Yemin GuoAbstract:Herein, we developed an Aptasensor using double-stranded DNA (dsDNA) modified with cadmium sulfide (CdS) nanoparticles and gold nanoparticles (AuNPs) on a gold electrode (GE) for kanamycin detection. The CdS nanoparticles were employed to strongly adsorb on the surface of GE via Au–S interactions. AuNPs, as the mediators, improved electron relay during the entire electron transfer process and the Aptasensor response speed. Herein, we used dsDNA instead of single-stranded DNA (ssDNA) as the capture probe to prepare an Aptasensor with improved stability. The proposed Aptasensor exhibited a wider linearity to kanamycin in the range of 10.0–450.0 nM with a low detection limit of 2.85 nM. The Aptasensor with ssDNA showed a low limit of detection of 9.76 nM. Moreover, it displayed high specificity for kanamycin and was free from interference in common milk adulterants. The proposed Aptasensor had good reproducibility, stability, repeatability, and cost-effective regeneration. The Aptasensor could selectively identify targets even in complex matrices, such as skimmed milk, and could be used for the detection of kanamycin in milk.
Seyed Mohammad Taghdisi - One of the best experts on this subject based on the ideXlab platform.
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Determination of microcystin-LR, employing Aptasensors.
Biosensors & bioelectronics, 2018Co-Authors: Hasan Badie Bostan, Seyed Mohammad Taghdisi, Jenna L. Bowen, Nikolaos Demertzis, Ramin Rezaee, Yunes Panahi, Aristidis Tsatsakis, Gholamreza KarimiAbstract:Cyanobacteria produce toxins such as microcystin-LR (MC-LR), which are associated with potential hepatotoxicity in humans. The detection of cyanobacteria and their toxins in drinking water and sea food is therefore crucial. To date, methods such as high performance liquid chromatography (HPLC), protein phosphatase inhibition assay (PPIA), and Raman spectroscopy have been employed to monitor MC-LR levels. Although these techniques are precise and sensitive, they require expensive instrumentation, well-trained personnel and involve time-consuming processes meaning that their application is generally limited to well-resourced, centralised laboratory facilities. Among the emerging MC-LR detection methods, Aptasensors have received great attention because of their remarkable sensitivity, selectivity, and simplicity. Aptamers, also known as "chemical" or "artificial antibodies", serve as the recognition moieties in Aptasensors. This review explores the current state-of-the-art of MC-LR Aptasensor platforms, evaluating the advantages and, limitations of typical transduction technologies to identify the most efficient detection system for the potentially harmful cyanobacteria associated toxin.
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Ultrasensitive detection of ochratoxin A using Aptasensors.
Biosensors & bioelectronics, 2017Co-Authors: Hasan Badie Bostan, Mohammad Ramezani, Khalil Abnous, Noor Mohammad Danesh, Gholamreza Karimi, Seyed Ali Mousavi Shaegh, Kazem Youssefi, Fahimeh Charbgoo, Seyed Mohammad TaghdisiAbstract:Regarding teratogenic, carcinogenic, and immunotoxic nature of ochratoxin A (OTA), selective and sensitive monitoring of this molecule in food samples is of great importance. In recent years, various methods have been introduced for detection of OTA. However, they are usually time-consuming, labor-intensive and expensive. Therefore, these parameters limited their usage. The emerging method of detection, Aptasensor, has attracted more attention for OTA detection, due to distinctive advantages including high sensitivity, selectivity and simplicity. In this review, the new developed Aptasensors for detection of OTA have been investigated. We also highlighted advantages and disadvantages of different types of OTA Aptasensors. This review also takes into consideration the goal to find out which designs are the most rational ones for highly sensitive detection of OTA.
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Optical and Electrochemical Aptasensors for Sensitive Detection of Streptomycin in Blood Serum and Milk.
Methods in molecular biology (Clifton N.J.), 2017Co-Authors: Mohammad Ramezani, Khalil Abnous, Seyed Mohammad TaghdisiAbstract:Detection and quantitation of antibiotic residues in blood serum and foodstuffs are in great demand. We have developed Aptasensors for detection of streptomycin using electrochemical and optical methods. In the first method, an electrochemical Aptasensor was developed for sensitive and selective detection of streptomycin, based on combination of exonuclease I (Exo I), complementary strand of aptamer (CS), arch shaped structure of aptamer (Apt)-CS conjugate, and gold electrode. The designed electrochemical Aptasensor exhibited high selectivity toward streptomycin with a limit of detection (LOD) as low as 11.4 nM. Moreover, the developed electrochemical Aptasensor was successfully used to detect streptomycin in milk and serum with LODs of 14.1 and 15.3 nM, respectively. In the second method, fluorescence quenching and colorimetric Aptasensors were designed for detection of streptomycin based on aqueous gold nanoparticles (AuNPs) and double-stranded DNA (dsDNA). In the absence of streptomycin, aptamer/FAM-labeled complementary strand dsDNA is stable, resulting in the aggregation of AuNPs by salt bridge and an obvious color change from red to blue and strong emission of fluorescence. The colorimetric and fluorescence quenching Aptasensors showed excellent selectivity toward streptomycin with limit of detections as low as 73.1 and 47.6 nM, respectively. The presented Aptasensors were successfully used to detect streptomycin in milk and serum. For serum, LODs were determined to be 58.2 and 102.4 nM for fluorescence quenching and colorimetric Aptasensors, respectively. For milk, LODs were calculated to be 56.2 and 108.7 nM for fluorescence quenching and colorimetric Aptasensors, respectively.
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Aptasensors for quantitative detection of kanamycin
Biosensors & bioelectronics, 2016Co-Authors: Rezvan Yazdian Robati, Mohammad Ramezani, Khalil Abnous, Atefeh Arab, Fatemeh Alebooye Langroodi, Seyed Mohammad TaghdisiAbstract:Up till now, various techniques have been developed to detect kanamycin in biological samples. However, due to some problems involved in these methods including time-consuming, expensive equipment and high consumption of reagents, new strategies for detection and quantitative determination of kanamycin are needed. Aptamer-based biosensors with unique recognition capability have attracted more attention of scientists because of its rapid response, high sensitivity and simple fabrication. Hence, we summarized optical and electrochemical kanamycin Aptasensors and focuses on recent advances and modern techniques in Aptasensor-based kanamycin detection techniques in order to provide readers with an inclusive understanding of its improvement and progress.
Yemin Guo - One of the best experts on this subject based on the ideXlab platform.
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Novel electrochemical Aptasensor with dual signal amplification strategy for detection of acetamiprid.
The Science of the total environment, 2019Co-Authors: Shi Xiaojie, Yemin Guo, Sun Jianfei, Yao Yao, Huimin Liu, Jingcheng Huang, Xia SunAbstract:Abstract In this work, a novel dual signal amplification strategy for Aptasensor employing reduced graphene with silver nanoparticles and prussian blue-gold nanocomposites was developed for detection of acetamiprid. To improve the sensitivity of Aptasensors, reduced graphene oxide-silver nanoparticles (rGo-AgNPs) were modified on a bare glassy carbon electrode surface, which provided a large specific surface area for subsequent material immobilization and amplified current signal. The electrical signal output and sensitivity of the Aptasensor was significantly improved after the immobilization of prussian blue-gold nanoparticles (PB-AuNPs) as a catalyst for the redox reaction. The analysis experiment exhibited that it had super-high sensitivity with a detection limit of 0.30 pM (S/N = 3), which met the requirements of the vast majority of daily leaf vegetable testing. Under optimized conditions, the proposed Aptasensor showed a wide linear detection range from 1 pM to 1 μM. This Aptasensor also had good stability and high selectivity for acetamiprid detection without an interfering effect of some other pesticides. The proposed Aptasensor displayed good recovery rates in real samples, which proposed a new method for constructing electrochemical sensors and provided a novel tool for rapid, sensitive analysis of pesticides with low cost.
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a dual signal amplification strategy for kanamycin based on ordered mesoporous carbon chitosan gold nanoparticles streptavidin and ferrocene labelled dna
Analytica Chimica Acta, 2018Co-Authors: Yemin Guo, Xia Sun, Xiangyou Wang, Wenping ZhaoAbstract:Abstract An ultrasensitive electrochemical Aptasensor for kanamycin (KAN) detection was constructed with a dual-signal amplification strategy. The Aptasensor achieved greatly amplified sensitivity due to the excellent electrical conductivity of the ordered mesoporous carbon-chitosan (OMC-CS)/gold nanoparticles-streptavidin (AuNPs-SA) and DNA2 labelled with ferrocene (Fc-DNA2). The AuNPs-SA was used to immobilize the DNA strand (biotin labelled) with the biotin-streptavidin system. The DNA2 strand containing the KAN aptamer was labelled with ferrocene to increase the current signal on the electrode surface when bound to KAN. Some factors that affect the performance of the Aptasensor were optimized, and the proposed Aptasensor provided a wide linear range from 1 × 10−10 M to 4 × 10−6 M, with a detection limit as low as 35.69 pM for KAN under the optimized conditions. This Aptasensor had satisfactory electrochemical performance with good stability, sensitivity and reproducibility. Additionally, it also displayed a good specificity for KAN without interference from competitive analogues. Furthermore, the constructed Aptasensor was successfully used to detect KAN in a real milk sample. The proposed method for KAN detection has great potential for the detection of other antibiotics.
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Ratiometric electrochemical Aptasensor based on ferrocene and carbon nanofibers for highly specific detection of tetracycline residues.
Scientific reports, 2017Co-Authors: Xu Qingcui, Zengning Liu, Zhao Wenping, Yemin Guo, Xia Sun, Haiyun ZhangAbstract:A sensitive and efficient ratiometric electrochemical Aptasensor was designed for tetracycline (TET) detection in milk. The ratiometric electrochemical Aptasensor was constructed by integrating two Aptasensors termed as Aptasensor 1 and Aptasensor 2. The Aptasensor 1 was fabricated that based on ferrocene (Fc) and gold nanoparticles (AuNPs) nanocomposite. Meanwhile, the Aptasensor 2 was prepared that based on carbon nanofibers (CNFs) and AuNPs nanocomposite. TET-aptamer was immobilized effectively onto screen-printed carbon electrodes (SPCEs) surface through forming Au-S bond between AuNPs and thiol of aptamer at 5′ end to construct the Aptasensor 1 and Aptasensor 2. And their detection results were calculated by ratio. Thus, the proposed ratiometric Aptasensor solved the problem of low accuracy and large differences between batches. Under the optimized conditions, the TET was detected by differential pulse voltammetry (DPV). Taken advantage of ratio calculation, the as-prepared ratiometric Aptasensor could detect TET quantitatively in the range of 10−8–10−3gL−1, with a detection limit of 3.3 × 10−7gL−1. Moreover, its applicability to TET-contaminated real samples (milk) showed an excellent agreement with the values determined by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-ESI-MS/MS). With high sensitivity, accuracy and reliability, the developed ratiometric Aptasensor held a great potential in TET detection for food safety.
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An Aptasensor with dsDNA for rapid and highly sensitive detection of kanamycin in milk
RSC Advances, 2017Co-Authors: Li Falan, Xia Sun, Xiangyou Wang, Yemin GuoAbstract:Herein, we developed an Aptasensor using double-stranded DNA (dsDNA) modified with cadmium sulfide (CdS) nanoparticles and gold nanoparticles (AuNPs) on a gold electrode (GE) for kanamycin detection. The CdS nanoparticles were employed to strongly adsorb on the surface of GE via Au–S interactions. AuNPs, as the mediators, improved electron relay during the entire electron transfer process and the Aptasensor response speed. Herein, we used dsDNA instead of single-stranded DNA (ssDNA) as the capture probe to prepare an Aptasensor with improved stability. The proposed Aptasensor exhibited a wider linearity to kanamycin in the range of 10.0–450.0 nM with a low detection limit of 2.85 nM. The Aptasensor with ssDNA showed a low limit of detection of 9.76 nM. Moreover, it displayed high specificity for kanamycin and was free from interference in common milk adulterants. The proposed Aptasensor had good reproducibility, stability, repeatability, and cost-effective regeneration. The Aptasensor could selectively identify targets even in complex matrices, such as skimmed milk, and could be used for the detection of kanamycin in milk.
Tibor Hianik - One of the best experts on this subject based on the ideXlab platform.
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label free electrochemical Aptasensor for cytochrome c detection using pillar 5 arene bearing neutral red
Sensors and Actuators B-chemical, 2016Co-Authors: Gennady Evtugyn, Tibor Hianik, Ivan I Stoikov, V B Stepanova, D N Shurpik, Vladimir G Evtugyn, Yu N OsinAbstract:Abstract Novel electrochemical Aptasensor toward cytochrome c (Cyt c ) has been developed on the base of glassy carbon electrode (GCE) modified with electropolymerized neutral red (Poly-NR) and decacarboxylated pillar[5]arene (P[5]A-COOH) bearing terminal neutral red (NR) and aminated aptamer specific to Cyt c . Addition of Cyt c resulted in decrease of the cathodic peak current of NR on cyclic voltammogram due to suppression of the electron exchange between reduced and oxidized NR forms in the surface layer. The implementation of Cyt c in the surface layer was confirmed by scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). Depending on the content of the surface layer and assembling protocol, the limits of detection (LODs) varied from 0.02 to 1.0 nM and linear range of concentrations was within three orders of magnitude. Interfering influence of some proteins and polyethylene glycol was characterized. The Aptasensors developed can find application in detection of Cyt c as apoptosis agent in blood serum. This has been partially validated in model blood serum mimicking the ionic composition of the plasma.
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electrochemical Aptasensor based on polycarboxylic macrocycle modified with neutral red for aflatoxin b1 detection
Electroanalysis, 2014Co-Authors: Gennady Evtugyn, Anna Porfireva, Veronika Stepanova, Rusal Sitdikov, Ivan I Stoikov, Dimitrios P Nikolelis, Tibor HianikAbstract:Novel electrochemical Aptasensors based on glassy carbon electrodes modified with electropolymer- ized Neutral red and polycarboxylated macrocyclic li- gands onto which the DNA aptamers were covalently at- tached have been developed for detection of Aflatoxin B1 (AFB1). The interaction with an analyte resulted in the decrease of the cathodic peak current of the probe measured by CV and in the increase of the electron trans- fer resistance determined by EIS. The limit of detection was found to be 0.1 nM for CV and 0.05 nM for EIS methods, respectively. The Aptasensor makes it possible to detect AFB1 in peanuts, cashew nuts, white wine and soy sauce with a recovery of 85-100 %.
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Polyphenothiazine modified electrochemical Aptasensor for detection of human α-thrombin
Electroanalysis, 2007Co-Authors: Anna Porfirieva, Gennady Evtugyn, Tibor HianikAbstract:QCM Aptasensor for detection human thrombin has been developed on the base of polymeric forms of phenothiazine dyes, Methylene Blue and Methylene Green. Electrostatic accumulation of the analyte in the polyphenothiazine layer made it possible to increase the sensitivity of QCM detection of thrombin in comparison with bare gold electrodes coated with avidin or neutravidin. The influence of nonspecific binding of human serum albumin and the optimal composition of the surface layers were determined. The Aptasensors developed make it possible to detect 10-100 nM of thrombin. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
Ruo Yuan - One of the best experts on this subject based on the ideXlab platform.
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target induced structure switching of aptamers facilitates strand displacement for dnazyme recycling amplification detection of thrombin in human serum
Analyst, 2019Co-Authors: Jin Li, Sujing Wang, Bingying Jiang, Yun Xiang, Ruo YuanAbstract:To monitor the thrombin concentration under the condition of abnormal blood coagulation is of clinical significance for the diagnosis of various diseases. Here, on the basis of the aptamer structure switching induced by the target molecules and the signal amplification strategy via recycling of metal-ion dependent DNAzymes, we have established a sensitive and simple fluorescent Aptasensor for detecting thrombin in human serum. The thrombin target specifically binds to the aptamer sequence and causes a corresponding conformational structure switching, which leads to the formation of a toehold sequence to facilitate the strand migration displacement reaction for the generation of functional metal-ion dependent DNAzymes. These DNAzymes further cleave the fluorescently quenched hairpin substrates cyclically to yield substantially amplified fluorescence recovery for sensitively detecting thrombin in the dynamic range from 0.01 nM to 50 nM. Such an Aptasensor shows a detection limit of 6.9 pM and can achieve the monitoring of thrombin in diluted human serum with high selectivity, offering a universal sensing strategy for the construction of various sensitive and simple Aptasensors to detect different biomarker molecules.
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an amplified electrochemical Aptasensor for thrombin detection based on pseudobienzymic fe3o4 au nanocomposites and electroactive hemin g quadruplex as signal enhancers
Analyst, 2014Co-Authors: Pei Jing, Lijuan Bai, Ruo YuanAbstract:A sensitive and selective electrochemical Aptasensor for thrombin detection was constructed based on hemin/G-quadruplex as the signal label and Fe3O4–Au nanocomposites with glucose oxidase (GOx-) and peroxide-mimicking enzyme activity as the signal enhancers. Due to their large surface area and good biocompatibility, Fe3O4–Au nanocomposites were employed to immobilize electroactive hemin/G-quadruplex, which was formed by the conjugation between a single-stranded guanine-rich nucleic acid and hemin. Based on the GOx-mimicking enzyme activity, Au nanoparticles on the surface of the Fe3O4–Au nanocomposites effectively catalyzed the oxidization of glucose in the presence of dissolved O2, accompanied by the production of H2O2. Both the Fe3O4 cores of Fe3O4–Au nanocomposites and hemin/G-quadruplex with H2O2-mimicking enzyme activity could catalyze the reduction of the generated H2O2, which promoted the electron transfer of hemin and amplified the electrochemical signal. The proposed electrochemical Aptasensor had a wide dynamic linear range of 0.1 pM to 20 nM with a lower detection limit of 0.013 pM, which provided a promising method for a sensitive assay for the detection of proteins in electrochemical Aptasensors.