The Experts below are selected from a list of 23637 Experts worldwide ranked by ideXlab platform
Seyed Mohammad Taghdisi - One of the best experts on this subject based on the ideXlab platform.
-
a label free fluorescent aptasensor for detection of Kanamycin based on dsdna capped mesoporous silica nanoparticles and rhodamine b
Analytica Chimica Acta, 2018Co-Authors: Shahrzad Dehghani, Mohammad Ramezani, Khalil Abnous, Noor Mohammad Danesh, Parirokh Lavaee, Mona Alibolandi, Mojgan Nejabat, Seyed Mohammad TaghdisiAbstract:Abstract Kanamycin is an aminoglycoside antibiotic that can be useful against both gram negative and positive bacteria. However, if its serum levels are not controlled properly, it can cause serious side effects like ototoxicity and nephrotoxicity. The aim of this study was to design a simple and rapid fluorescent aptasensor for detection of Kanamycin, based on Aptamer/Complementary strand (dsDNA)-capped mesoporous silica nanoparticles (MSNs) and Rhodamine B as a fluorescent probe. The MSNs pores were filled with Rhodamine B and then gated with dsDNA. In the presence of Kanamycin, the aptamer sequence was separated from its complementary strand (CS), so that, uncovered the pores and leading to leakage of Rhodamine B. Thus, a significant increase in the fluorescence intensity was observed. The relative fluorescence intensity showed a linearity range from 24.75 nM to 137.15 nM of Kanamycin with a detection limit of 7.5 nM. The aptasensor also showed to be useful for detection of Kanamycin in serum samples and was able to distinguish Kanamycin from other antibiotics, resulting in a sensitive, rapid and inexpensive method for Kanamycin detection.
-
Aptasensors for quantitative detection of Kanamycin
Biosensors & bioelectronics, 2016Co-Authors: Rezvan Yazdian Robati, Mohammad Ramezani, Khalil Abnous, Atefeh Arab, Fatemeh Alebooye Langroodi, Seyed Mohammad TaghdisiAbstract:Up till now, various techniques have been developed to detect Kanamycin in biological samples. However, due to some problems involved in these methods including time-consuming, expensive equipment and high consumption of reagents, new strategies for detection and quantitative determination of Kanamycin are needed. Aptamer-based biosensors with unique recognition capability have attracted more attention of scientists because of its rapid response, high sensitivity and simple fabrication. Hence, we summarized optical and electrochemical Kanamycin aptasensors and focuses on recent advances and modern techniques in aptasensor-based Kanamycin detection techniques in order to provide readers with an inclusive understanding of its improvement and progress.
-
a selective and sensitive fluorescent aptasensor for detection of Kanamycin based on catalytic recycling activity of exonuclease iii and gold nanoparticles
Sensors and Actuators B-chemical, 2016Co-Authors: Mohammad Ramezani, Khalil Abnous, Noor Mohammad Danesh, Parirokh Lavaee, Seyed Mohammad TaghdisiAbstract:Abstract Kanamycin is an aminoglycoside antibiotic used in human and veterinary medicine. Sensitive and selective methods to detect Kanamycin residues for food safety and clinical diagnosis are of great interest. In this study a fluorescent aptasensor was designed to detect Kanamycin based on exonuclease III activity (Exo III), gold nanoparticles (AuNPs) and FAM-Labeled complimentary strand of aptamer (CS). In the absence of Kanamycin, aptamer binds to its CS to form a double-stranded DNA (dsDNA) with 3′-overhang end at aptamer and 3′-blunt end at CS. The formed dsDNA leaves the surface of AuNPs. Upon addition of Exo III, aptamer is recycled from dsDNA and the cycle goes on, leading to a very strong fluorescence emission. In the presence of Kanamycin, aptamer binds to its target and CS remains on the surface of AuNPs, resulting in a weak fluorescence emission. The designed aptasensor showed high selectivity toward aminoglycoside antibiotics, including Kanamycin and gentamicin, with a limit of detection (LOD) as low as 321 pM for Kanamycin. This aptasensor was successfully used to detect Kanamycin in milk and serum.
-
detection of Kanamycin by using an aptamer based biosensor using silica nanoparticles
Analytical Methods, 2015Co-Authors: Mohammad Ramezani, Khalil Abnous, Ladan Saadat Khabbaz, Mohammad Hassanzadehkhayyat, Pouya Zaree, Seyed Mohammad TaghdisiAbstract:A fluorescent aptasensor system has been designed for the sensitive detection of Kanamycin based on silica nanoparticles (SNPs) coated with streptavidin. The Kanamycin aptamer, which served as the molecular recognition probe, was immobilized on the surface of the SNPs. In the absence of Kanamycin, the SNPdouble stranded DNA (dsDNA) complex is intact and has the maximum fluorescent signal. Upon addition of Kanamycin, the aptamer binds to its target and causes the dissociation of the labeled-complementary strand from dsDNA and SNPs, leading to a decrease of the fluorescence intensity. This aptasensor exhibited a high sensitivity toward Kanamycin with a limit of detection (LOD) as low as 612 pM. The designed aptasensor was successfully used to detect Kanamycin in serum and a limit of detection as low as 453 pM was obtained. By changing the related aptamer strand and its complementary strand, it could be expected that the proposed method offers a general sensing platform for the recognition of trace amounts of different drugs and biomolecules.
Xuanxian Peng - One of the best experts on this subject based on the ideXlab platform.
-
fructose restores susceptibility of multidrug resistant edwardsiella tarda to Kanamycin
Journal of Proteome Research, 2015Co-Authors: Bo Peng, Yi Han, Xuanxian PengAbstract:Edwardsiella tarda, the causative agent of Edwardsiellosis, imposes medical challenges in both the clinic and aquaculture. The emergence of multidrug resistant strains makes antibiotic treatment impractical. The identification of molecules that facilitate or promote antibiotic efficacy is in high demand. In the present study, we aimed to identify small molecules whose abundance is correlated with Kanamycin resistance in E. tarda by gas chromatography–mass spectrometry. We found that the abundance of fructose was greatly suppressed in Kanamycin-resistant strains. The incubation of Kanamycin-resistant bacteria with exogenous fructose sensitized the bacteria to Kanamycin. Moreover, the fructose also functioned in bacteria persisters and biofilm. The synergistic effects of fructose and Kanamycin were validated in a mouse model. Furthermore, the mechanism relies on fructose in activating TCA cycle to produce NADH, which generates proton motive force to increase the uptake of the antibiotics. Therefore, we pres...
-
fructose restores susceptibility of multidrug resistant edwardsiella tarda to Kanamycin
Journal of Proteome Research, 2015Co-Authors: Bo Peng, Yi Han, Xuanxian PengAbstract:Edwardsiella tarda, the causative agent of Edwardsiellosis, imposes medical challenges in both the clinic and aquaculture. The emergence of multidrug resistant strains makes antibiotic treatment impractical. The identification of molecules that facilitate or promote antibiotic efficacy is in high demand. In the present study, we aimed to identify small molecules whose abundance is correlated with Kanamycin resistance in E. tarda by gas chromatography-mass spectrometry. We found that the abundance of fructose was greatly suppressed in Kanamycin-resistant strains. The incubation of Kanamycin-resistant bacteria with exogenous fructose sensitized the bacteria to Kanamycin. Moreover, the fructose also functioned in bacteria persisters and biofilm. The synergistic effects of fructose and Kanamycin were validated in a mouse model. Furthermore, the mechanism relies on fructose in activating TCA cycle to produce NADH, which generates proton motive force to increase the uptake of the antibiotics. Therefore, we present a novel approach in fighting against multidrug resistant bacteria through exploration of antibiotic-suppressed molecules.
Qin Wei - One of the best experts on this subject based on the ideXlab platform.
-
label free immunosensor for the detection of Kanamycin using ag fe3o4 nanoparticles and thionine mixed graphene sheet
Biosensors and Bioelectronics, 2013Co-Authors: Qin Wei, Liangguo Yan, Yong ZhangAbstract:Abstract A highly sensitive label-free immunosensor for the detection of Kanamycin had been developed using silver hybridized mesoporous ferroferric oxide nanoparticles (Ag@Fe 3 O 4 NPs) and thionine mixed graphene sheet (TH-GS). TH was used as an electron transfer mediator. The electrical signal was greatly improved in the presence of GS due to its good electron-transfer ability. With the advantages of large specific surface area and excellent electrical conductivity, Ag@Fe 3 O 4 NPs could immobilize more antibodies of Kanamycin and promote the electron transfer. Cyclic voltammetry and square wave voltammetry were used to characterize the recognition of Kanamycin. The proposed immunosensor showed good performances such as low detection limit (15 pg mL −1 ), wide linear range (from 0.050 to 16 ng mL −1 ), short analysis time (3 min), high stability, and good selectivity in the detection of Kanamycin. The immunosensor was evaluated for pork meat sample, receiving satisfactory results.
-
ultrasensitive detection of Kanamycin in animal derived foods by label free electrochemical immunosensor
Food Chemistry, 2012Co-Authors: Qin Wei, Yanfang Zhao, Minghui YangAbstract:A label-free amperometric immunosensor based on graphene sheet(GS)-nafion(Nf)/thionine(TH)/Pt nanoparticles modified electrode is proposed for the ultrasensitive detection of Kanamycin. The anti-Kanamycin antibody was immobilised onto the GS-Nf/TH/Pt modified glassy carbon electrode (GCE) through electrostatic adsorption. TH was selected as an electron transfer mediator, and the synergistic effects among GS, TH and Pt were investigated. The electroactivity of TH was greatly improved in the presence of GS and Pt due to their good electron-transfer ability. The proposed immunosensor shows good performance such as low detection limit (5.74 pg/mL), wide linear range (from 0.01 to 12.0 ng/mL), high stability, and good selectivity in the detection of Kanamycin. The reliability of the developed immunosensor was proved by determination of Kanamycin in animal derived foods with satisfactory results.
-
label free electrochemical immunosensor for sensitive detection of Kanamycin
Sensors and Actuators B-chemical, 2011Co-Authors: Yanfang Zhao, Qin Wei, Yanyan Cai, Kexia Mao, Zhentao CuiAbstract:Abstract A novel label-free electrochemical immunosensor for sensitive detection of Kanamycin based on water-soluble graphene sheet (WGS)/prussian blue-chitosan (PB-CTS)/nanoporous gold (NPG) composited film has been reported. PB was selected as an electron transfer mediator, and was modified onto the electrode together with WGS through electrostatic adsorption. Then NPG was immobilized onto the as-prepared film for biomolecules anchoring. The electroactivity of PB was greatly enhanced in the presence of WGS and NPG. It could mainly be ascribed to the fact that the good conductivity of WGS and NPG promoted electron transfer and enhanced the sensitivity. Kanamycin antibody, as a model, was immobilized onto the composite film for the detection of Kanamycin. Under optimum conditions, the amperometric signal of PB decreased linearly with Kanamycin concentration (0.02–14 ng mL −1 ), a linear calibration plot ( y = 1.3817 + 4.7544 x , r = 0.9993), resulting in a low limit of detection (6.31 pg mL −1 ). The novel immunosensor for the detection of Kanamycin in real sample with satisfactory results has been proved. In addition, this method would be easily adapted for the detection of other residual antibiotics in animal derived foods.
Mohammad Ramezani - One of the best experts on this subject based on the ideXlab platform.
-
a label free fluorescent aptasensor for detection of Kanamycin based on dsdna capped mesoporous silica nanoparticles and rhodamine b
Analytica Chimica Acta, 2018Co-Authors: Shahrzad Dehghani, Mohammad Ramezani, Khalil Abnous, Noor Mohammad Danesh, Parirokh Lavaee, Mona Alibolandi, Mojgan Nejabat, Seyed Mohammad TaghdisiAbstract:Abstract Kanamycin is an aminoglycoside antibiotic that can be useful against both gram negative and positive bacteria. However, if its serum levels are not controlled properly, it can cause serious side effects like ototoxicity and nephrotoxicity. The aim of this study was to design a simple and rapid fluorescent aptasensor for detection of Kanamycin, based on Aptamer/Complementary strand (dsDNA)-capped mesoporous silica nanoparticles (MSNs) and Rhodamine B as a fluorescent probe. The MSNs pores were filled with Rhodamine B and then gated with dsDNA. In the presence of Kanamycin, the aptamer sequence was separated from its complementary strand (CS), so that, uncovered the pores and leading to leakage of Rhodamine B. Thus, a significant increase in the fluorescence intensity was observed. The relative fluorescence intensity showed a linearity range from 24.75 nM to 137.15 nM of Kanamycin with a detection limit of 7.5 nM. The aptasensor also showed to be useful for detection of Kanamycin in serum samples and was able to distinguish Kanamycin from other antibiotics, resulting in a sensitive, rapid and inexpensive method for Kanamycin detection.
-
Aptasensors for quantitative detection of Kanamycin
Biosensors & bioelectronics, 2016Co-Authors: Rezvan Yazdian Robati, Mohammad Ramezani, Khalil Abnous, Atefeh Arab, Fatemeh Alebooye Langroodi, Seyed Mohammad TaghdisiAbstract:Up till now, various techniques have been developed to detect Kanamycin in biological samples. However, due to some problems involved in these methods including time-consuming, expensive equipment and high consumption of reagents, new strategies for detection and quantitative determination of Kanamycin are needed. Aptamer-based biosensors with unique recognition capability have attracted more attention of scientists because of its rapid response, high sensitivity and simple fabrication. Hence, we summarized optical and electrochemical Kanamycin aptasensors and focuses on recent advances and modern techniques in aptasensor-based Kanamycin detection techniques in order to provide readers with an inclusive understanding of its improvement and progress.
-
a selective and sensitive fluorescent aptasensor for detection of Kanamycin based on catalytic recycling activity of exonuclease iii and gold nanoparticles
Sensors and Actuators B-chemical, 2016Co-Authors: Mohammad Ramezani, Khalil Abnous, Noor Mohammad Danesh, Parirokh Lavaee, Seyed Mohammad TaghdisiAbstract:Abstract Kanamycin is an aminoglycoside antibiotic used in human and veterinary medicine. Sensitive and selective methods to detect Kanamycin residues for food safety and clinical diagnosis are of great interest. In this study a fluorescent aptasensor was designed to detect Kanamycin based on exonuclease III activity (Exo III), gold nanoparticles (AuNPs) and FAM-Labeled complimentary strand of aptamer (CS). In the absence of Kanamycin, aptamer binds to its CS to form a double-stranded DNA (dsDNA) with 3′-overhang end at aptamer and 3′-blunt end at CS. The formed dsDNA leaves the surface of AuNPs. Upon addition of Exo III, aptamer is recycled from dsDNA and the cycle goes on, leading to a very strong fluorescence emission. In the presence of Kanamycin, aptamer binds to its target and CS remains on the surface of AuNPs, resulting in a weak fluorescence emission. The designed aptasensor showed high selectivity toward aminoglycoside antibiotics, including Kanamycin and gentamicin, with a limit of detection (LOD) as low as 321 pM for Kanamycin. This aptasensor was successfully used to detect Kanamycin in milk and serum.
-
detection of Kanamycin by using an aptamer based biosensor using silica nanoparticles
Analytical Methods, 2015Co-Authors: Mohammad Ramezani, Khalil Abnous, Ladan Saadat Khabbaz, Mohammad Hassanzadehkhayyat, Pouya Zaree, Seyed Mohammad TaghdisiAbstract:A fluorescent aptasensor system has been designed for the sensitive detection of Kanamycin based on silica nanoparticles (SNPs) coated with streptavidin. The Kanamycin aptamer, which served as the molecular recognition probe, was immobilized on the surface of the SNPs. In the absence of Kanamycin, the SNPdouble stranded DNA (dsDNA) complex is intact and has the maximum fluorescent signal. Upon addition of Kanamycin, the aptamer binds to its target and causes the dissociation of the labeled-complementary strand from dsDNA and SNPs, leading to a decrease of the fluorescence intensity. This aptasensor exhibited a high sensitivity toward Kanamycin with a limit of detection (LOD) as low as 612 pM. The designed aptasensor was successfully used to detect Kanamycin in serum and a limit of detection as low as 453 pM was obtained. By changing the related aptamer strand and its complementary strand, it could be expected that the proposed method offers a general sensing platform for the recognition of trace amounts of different drugs and biomolecules.
Khalil Abnous - One of the best experts on this subject based on the ideXlab platform.
-
a label free fluorescent aptasensor for detection of Kanamycin based on dsdna capped mesoporous silica nanoparticles and rhodamine b
Analytica Chimica Acta, 2018Co-Authors: Shahrzad Dehghani, Mohammad Ramezani, Khalil Abnous, Noor Mohammad Danesh, Parirokh Lavaee, Mona Alibolandi, Mojgan Nejabat, Seyed Mohammad TaghdisiAbstract:Abstract Kanamycin is an aminoglycoside antibiotic that can be useful against both gram negative and positive bacteria. However, if its serum levels are not controlled properly, it can cause serious side effects like ototoxicity and nephrotoxicity. The aim of this study was to design a simple and rapid fluorescent aptasensor for detection of Kanamycin, based on Aptamer/Complementary strand (dsDNA)-capped mesoporous silica nanoparticles (MSNs) and Rhodamine B as a fluorescent probe. The MSNs pores were filled with Rhodamine B and then gated with dsDNA. In the presence of Kanamycin, the aptamer sequence was separated from its complementary strand (CS), so that, uncovered the pores and leading to leakage of Rhodamine B. Thus, a significant increase in the fluorescence intensity was observed. The relative fluorescence intensity showed a linearity range from 24.75 nM to 137.15 nM of Kanamycin with a detection limit of 7.5 nM. The aptasensor also showed to be useful for detection of Kanamycin in serum samples and was able to distinguish Kanamycin from other antibiotics, resulting in a sensitive, rapid and inexpensive method for Kanamycin detection.
-
Aptasensors for quantitative detection of Kanamycin
Biosensors & bioelectronics, 2016Co-Authors: Rezvan Yazdian Robati, Mohammad Ramezani, Khalil Abnous, Atefeh Arab, Fatemeh Alebooye Langroodi, Seyed Mohammad TaghdisiAbstract:Up till now, various techniques have been developed to detect Kanamycin in biological samples. However, due to some problems involved in these methods including time-consuming, expensive equipment and high consumption of reagents, new strategies for detection and quantitative determination of Kanamycin are needed. Aptamer-based biosensors with unique recognition capability have attracted more attention of scientists because of its rapid response, high sensitivity and simple fabrication. Hence, we summarized optical and electrochemical Kanamycin aptasensors and focuses on recent advances and modern techniques in aptasensor-based Kanamycin detection techniques in order to provide readers with an inclusive understanding of its improvement and progress.
-
a selective and sensitive fluorescent aptasensor for detection of Kanamycin based on catalytic recycling activity of exonuclease iii and gold nanoparticles
Sensors and Actuators B-chemical, 2016Co-Authors: Mohammad Ramezani, Khalil Abnous, Noor Mohammad Danesh, Parirokh Lavaee, Seyed Mohammad TaghdisiAbstract:Abstract Kanamycin is an aminoglycoside antibiotic used in human and veterinary medicine. Sensitive and selective methods to detect Kanamycin residues for food safety and clinical diagnosis are of great interest. In this study a fluorescent aptasensor was designed to detect Kanamycin based on exonuclease III activity (Exo III), gold nanoparticles (AuNPs) and FAM-Labeled complimentary strand of aptamer (CS). In the absence of Kanamycin, aptamer binds to its CS to form a double-stranded DNA (dsDNA) with 3′-overhang end at aptamer and 3′-blunt end at CS. The formed dsDNA leaves the surface of AuNPs. Upon addition of Exo III, aptamer is recycled from dsDNA and the cycle goes on, leading to a very strong fluorescence emission. In the presence of Kanamycin, aptamer binds to its target and CS remains on the surface of AuNPs, resulting in a weak fluorescence emission. The designed aptasensor showed high selectivity toward aminoglycoside antibiotics, including Kanamycin and gentamicin, with a limit of detection (LOD) as low as 321 pM for Kanamycin. This aptasensor was successfully used to detect Kanamycin in milk and serum.
-
detection of Kanamycin by using an aptamer based biosensor using silica nanoparticles
Analytical Methods, 2015Co-Authors: Mohammad Ramezani, Khalil Abnous, Ladan Saadat Khabbaz, Mohammad Hassanzadehkhayyat, Pouya Zaree, Seyed Mohammad TaghdisiAbstract:A fluorescent aptasensor system has been designed for the sensitive detection of Kanamycin based on silica nanoparticles (SNPs) coated with streptavidin. The Kanamycin aptamer, which served as the molecular recognition probe, was immobilized on the surface of the SNPs. In the absence of Kanamycin, the SNPdouble stranded DNA (dsDNA) complex is intact and has the maximum fluorescent signal. Upon addition of Kanamycin, the aptamer binds to its target and causes the dissociation of the labeled-complementary strand from dsDNA and SNPs, leading to a decrease of the fluorescence intensity. This aptasensor exhibited a high sensitivity toward Kanamycin with a limit of detection (LOD) as low as 612 pM. The designed aptasensor was successfully used to detect Kanamycin in serum and a limit of detection as low as 453 pM was obtained. By changing the related aptamer strand and its complementary strand, it could be expected that the proposed method offers a general sensing platform for the recognition of trace amounts of different drugs and biomolecules.
