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Aqueous Humour

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Evangelia E Tsironi – 1st expert on this subject based on the ideXlab platform

  • plasma and Aqueous Humour levels of ghrelin in open angle glaucoma patients
    Clinical and Experimental Ophthalmology, 2011
    Co-Authors: Andreas Katsanos, Anna Dastiridou, Panagiotis Georgoulias, Pierros Cholevas, Maria G Kotoula, Evangelia E Tsironi

    Abstract:

    Background:  To compare Aqueous Humour and plasma levels of ghrelin, a peptide recently identified in human eyes, in patients with open-angle glaucoma and controls.

    Design:  Cross-sectional, controlled, hospital-based study.

    Participants:  Twenty-four open-angle glaucoma (17 primary open-angle and 7 pseudo-exfoliation glaucoma) patients and 30 controls were included. All participants were patients scheduled for cataract or glaucoma surgery. Patients with other ocular pathology, previous ocular surgery or diabetes were excluded.

    Methods:  Blood samples were collected before elective surgery. Aqueous Humour was aspirated from the anterior chamber through a paracentesis with a 27-G needle under sterile conditions before any tissue manipulation. Ghrelin quantification was performed with commercially available Radioimmunoassay kits.

    Main Outcome Measure:  Ghrelin levels in Aqueous Humour and plasma.

    Results:  Plasma levels of ghrelin were 490.5 ± 156.0 pg/mL in the open-angle glaucoma and 482.2 ± 125.4 pg/mL in the control group (Mann–Whitney test, P = 0.897). Aqueous Humour levels of ghrelin were 85.5 ± 15.4 and 123.4 ± 25.5 pg/mL in the respective groups (P < 0.001). The ratio of plasma/Aqueous Humour ghrelin concentration was higher in the open-angle glaucoma versus the control group (5.75 ± 1.92 vs. 4.00 ± 1.04, P < 0.001). There was no difference in Aqueous Humour levels of ghrelin between primary open-angle glaucoma and pseudo-exfoliation glaucoma patients (P = 0.494).

    Conclusions:  Aqueous Humour levels of ghrelin were significantly lower in open-angle glaucoma patients, compared with controls. This difference may manifest a role of ghrelin in the disease process or a consequence of antiglaucoma treatment.

  • Plasma and Aqueous Humour levels of ghrelin in open-angle glaucoma patients.
    Clinical and Experimental Ophthalmology, 2011
    Co-Authors: Andreas Katsanos, Anna Dastiridou, Panagiotis Georgoulias, Pierros Cholevas, Maria G Kotoula, Evangelia E Tsironi

    Abstract:

    Background:  To compare Aqueous Humour and plasma levels of ghrelin, a peptide recently identified in human eyes, in patients with open-angle glaucoma and controls.

    Design:  Cross-sectional, controlled, hospital-based study.

    Participants:  Twenty-four open-angle glaucoma (17 primary open-angle and 7 pseudo-exfoliation glaucoma) patients and 30 controls were included. All participants were patients scheduled for cataract or glaucoma surgery. Patients with other ocular pathology, previous ocular surgery or diabetes were excluded.

    Methods:  Blood samples were collected before elective surgery. Aqueous Humour was aspirated from the anterior chamber through a paracentesis with a 27-G needle under sterile conditions before any tissue manipulation. Ghrelin quantification was performed with commercially available Radioimmunoassay kits.

    Main Outcome Measure:  Ghrelin levels in Aqueous Humour and plasma.

    Results:  Plasma levels of ghrelin were 490.5 ± 156.0 pg/mL in the open-angle glaucoma and 482.2 ± 125.4 pg/mL in the control group (Mann–Whitney test, P = 0.897). Aqueous Humour levels of ghrelin were 85.5 ± 15.4 and 123.4 ± 25.5 pg/mL in the respective groups (P 

Masahito Ohji – 2nd expert on this subject based on the ideXlab platform

  • vascular endothelial growth factor in the Aqueous Humour in eyes with myopic choroidal neovascularization
    Acta Ophthalmologica, 2011
    Co-Authors: Osamu Sawada, Hajime Kawamura, Masashi Kakinoki, Tomoko Sawada, Masahito Ohji

    Abstract:

    .

    Purpose:  To determine the concentration of vascular endothelial growth factor (VEGF) in the Aqueous Humour of eyes with myopic choroidal neovascularization (mCNV).

    Methods:  Aqueous Humour samples were obtained from 21 eyes of 21 patients with mCNV and from 21 eyes of 21 patients with cataract without CNV or other ocular or systemic diseases (control group). The VEGF concentration in the Aqueous Humour was measured using an enzyme-linked immunosorbent assay.

    Results:  The VEGF concentrations in the Aqueous Humour of eyes with mCNV ranged from < 20.6 to 200 pg/ml (median 35 pg/ml). The concentrations in the control group ranged from 26 to 218 pg/ml (median 100 pg/ml). The difference between the two VEGF concentrations in the Aqueous Humour was significant (p < 0.001, Mann–Whitney rank sum test). Conclusion:  The VEGF concentration in the Aqueous Humour of patients with mCNV is lower than in normal controls. VEGF might localize in or around the CNV in eyes with mCNV.

  • Vascular endothelial growth factor in the Aqueous Humour in eyes with myopic choroidal neovascularization
    Acta Ophthalmologica, 2010
    Co-Authors: Osamu Sawada, Hajime Kawamura, Masashi Kakinoki, Tomoko Sawada, Masahito Ohji

    Abstract:

    .

    Purpose:  To determine the concentration of vascular endothelial growth factor (VEGF) in the Aqueous Humour of eyes with myopic choroidal neovascularization (mCNV).

    Methods:  Aqueous Humour samples were obtained from 21 eyes of 21 patients with mCNV and from 21 eyes of 21 patients with cataract without CNV or other ocular or systemic diseases (control group). The VEGF concentration in the Aqueous Humour was measured using an enzyme-linked immunosorbent assay.

    Results:  The VEGF concentrations in the Aqueous Humour of eyes with mCNV ranged from

Esko Aine – 3rd expert on this subject based on the ideXlab platform

  • determination of protein content in Aqueous Humour by high performance gel filtration chromatography
    Acta Ophthalmologica, 2009
    Co-Authors: K. M. Saari, Esko Aine, M T Parviainen

    Abstract:

    High-performance liquid chromatography (HPLC) was used to study the protein content of Aqueous Humour in 17 cataract patients. High-performance gel filtration chromatograms (HPGFC) of the Aqueous Humour showed 7–12 peaks with low concentrations (mean 0.085 μg/μl) of high molecular weight proteins (peak 1, MW 250 000 or more) and low levels (0.01 μg/μl or less) of IgA (peak 2) and of IgG (peak 3). The fourth peak (MW about 65 000) containing albumin and obviously also trasferrin was the major peak (mean 0.596 μg/μl) at HPGFC of a normal Aqueous Humour. Lysozyme (peak 6, MW 35 000) occurred in small amounts in the Aqueous Humour (mean 0.064 μg/μl). The last 6 peaks matched for peptides and amino acids (MW 10 000 or less). It is concluded that HPLC may be a useful preparative method in characterization, separation, and partial purification of immunoglobulins, immune complexes, and other proteins of Aqueous Humour.

  • Aqueous Humour flow after a single oral dose of isosorbide-5-mononitrate in healthy volunteers.
    Acta Ophthalmologica Scandinavica, 2003
    Co-Authors: Hanna Kotikoski, Olli Oksala, Heikki Vapaatalo, Esko Aine

    Abstract:

    .

    Purpose:  To investigate whether a nitric oxide donor given as a single oral dose is able to modify Aqueous Humour flow in healthy volunteers.

    Methods:  Ten healthy volunteers participated in a randomized, double-masked and placebo-controlled cross-over study. Aqueous Humour flow was assessed by fluorophotometry after intake of isosorbide-5-mononitrate (ISMN), 10 mg. Topical timolol maleate, which is known to reduce Aqueous Humour flow, was used as a positive control. Intraocular pressure (IOP) was measured by applanation tonometry and blood pressure was registered.

    Results:  The basal rate of Aqueous Humour flow did not change significantly after a single oral dose of ISMN. The Aqueous Humour flow in the timolol-treated eye was reduced as compared to the contralateral control eye (p = 0.002). Mean IOP 6 hours after placebo and ISMN intake did not differ significantly. Timolol lowered IOP by 4 mmHg (p 

  • biochemical markers of the l arginine nitric oxide pathway in the Aqueous Humour in glaucoma patients
    Acta Ophthalmologica Scandinavica, 2002
    Co-Authors: Hanna Kotikoski, Heikki Vapaatalo, Eeva Moilanen, Esko Aine

    Abstract:

    .

    Purpose:  To study the role of the L-arginine-nitric oxide (NO) pathway in Aqueous Humour dynamics by measuring nitrate, nitrite and cyclic (cGMP) levels in guanosine 3′,5′-monophosphate the Aqueous Humour of glaucomatous and nonglaucomatous cataract patients.

    Methods:  The study involved 38 glaucoma patients undergoing unilateral cataract surgery in the glaucomatous eye and 38 cataract control patients matched for sex, age, smoking habits and organic nitrate medication. All subjects underwent ophthalmic examination, and blood pressure was measured preoperatively. Nitrite, nitrate and cGMP levels were measured in Aqueous Humour and serum.

    Results:  The NOx (nitrite + nitrate), nitrite and cGMP concentrations in the Aqueous Humour were slightly higher in the glaucoma patients than in the control patients, but the differences did not reach statistical significance. The levels of cGMP in serum were higher in the glaucoma patients (P = 0.053). The subgroup of glaucoma patients with pseudoexfoliation had lower NOx and nitrite values in the Aqueous Humour (P = 0.046 and P = 0.345, respectively) than the matched controls, while cGMP levels were higher (P = 0.043). Levels of NOx and nitrite in the Aqueous Humour were higher in patients using oral nitroglycerin (P = 0.062 and P = 0.042, respectively) than in patients without this medication. Blood pressure was higher in the glaucoma patients, with a mean of 165/89 mmHg as compared to 153/81 mmHg in the controls (P-values 0.071/0.008).

    Conclusions:  No differences in NO metabolites were found between glaucoma and control patients. However, any real changes may have been disguised by optimal medication of glaucoma. Low NOx and high cGMP levels in the Aqueous Humour of pseudoexfoliation patients warrant further evaluation in a larger study.