The Experts below are selected from a list of 54564 Experts worldwide ranked by ideXlab platform

J.g Liu - One of the best experts on this subject based on the ideXlab platform.

  • Aqueous Two-Phase electrophoresis for separation of amino acids
    Separation and Purification Technology, 2001
    Co-Authors: S.l Zhai, G.s. Luo, J.g Liu
    Abstract:

    Two-Phase electrophoresis, coupling traditional solvent extraction with electrophoresis, is a novel separation technique. Being bio-compatible, Aqueous Two-Phase electrophoresis provides a successful method for separating mixtures of biomolecules. In this work, the separation of amino acids by Aqueous Two-Phase electrophoresis was examined with dextran-polyethylene glycol-water as a working system. The influences of separation time, field strength, initial solute concentration and Two-Phase contact area were studied in intermittent separation equipment. The experiment results show that Aqueous Two-Phase electrophoresis is an effective separation technique for amino acids. With time, field strength, and contact area increasing, mass fluxes across the interface are increased quickly.

  • Selective recovery of amino acids by Aqueous Two-Phase electrophoresis
    Chemical Engineering Journal, 2001
    Co-Authors: S.l Zhai, G.s. Luo, J.g Liu
    Abstract:

    Abstract Aqueous Two-Phase systems (ATPSs) formed by spontaneous Phase separation of different water-soluble polymers are bio-compatible. Aqueous Two-Phase electrophoresis, coupling traditional solvent extraction with electrophoresis, is a novel separation technique and provides a successful method for separating mixtures of biomolecules. In this work, the selective recovery of amino acids by Aqueous Two-Phase electrophoresis was examined with dextran–polyethylene glycol–water as a working system. The experiment results show that glutamic acid can be separated from phenylalanine and tryptophan successfully. The influences of the electrophoresis time, field strength, and Phase volume ratio were studied in an intermittent separation equipment.

S.l Zhai - One of the best experts on this subject based on the ideXlab platform.

  • Aqueous Two-Phase electrophoresis for separation of amino acids
    Separation and Purification Technology, 2001
    Co-Authors: S.l Zhai, G.s. Luo, J.g Liu
    Abstract:

    Two-Phase electrophoresis, coupling traditional solvent extraction with electrophoresis, is a novel separation technique. Being bio-compatible, Aqueous Two-Phase electrophoresis provides a successful method for separating mixtures of biomolecules. In this work, the separation of amino acids by Aqueous Two-Phase electrophoresis was examined with dextran-polyethylene glycol-water as a working system. The influences of separation time, field strength, initial solute concentration and Two-Phase contact area were studied in intermittent separation equipment. The experiment results show that Aqueous Two-Phase electrophoresis is an effective separation technique for amino acids. With time, field strength, and contact area increasing, mass fluxes across the interface are increased quickly.

  • Selective recovery of amino acids by Aqueous Two-Phase electrophoresis
    Chemical Engineering Journal, 2001
    Co-Authors: S.l Zhai, G.s. Luo, J.g Liu
    Abstract:

    Abstract Aqueous Two-Phase systems (ATPSs) formed by spontaneous Phase separation of different water-soluble polymers are bio-compatible. Aqueous Two-Phase electrophoresis, coupling traditional solvent extraction with electrophoresis, is a novel separation technique and provides a successful method for separating mixtures of biomolecules. In this work, the selective recovery of amino acids by Aqueous Two-Phase electrophoresis was examined with dextran–polyethylene glycol–water as a working system. The experiment results show that glutamic acid can be separated from phenylalanine and tryptophan successfully. The influences of the electrophoresis time, field strength, and Phase volume ratio were studied in an intermittent separation equipment.

Pau Loke Show - One of the best experts on this subject based on the ideXlab platform.

  • Current applications of different type of Aqueous Two-Phase systems
    Bioresources and Bioprocessing, 2015
    Co-Authors: Yee Koon Yau, Chien Wei Ooi, John Chi-wei Lan, Tau Chuan Ling, Pau Loke Show
    Abstract:

    In recent year, Aqueous Two-Phase system (ATPS) has become a proven tool used in separation and purification technology. The application of ATPSs in clarification, partitioning and partial purification of biomolecules and bioproducts had showed the rapid development. This method is able to give high recovery yield and high purity in a single step. The ATPS shows characteristics of high selectivity and is easily to scale up. Therefore, ATPS offers an attractive alternative that meets the requirements of the high demand in industry processes and it is also beneficial in terms of economic and environmental protection. In the past, a lot of works and researches have been done in order to develop feasible separation processes using different types of ATPSs and their applications in numerous product separations. This paper aims to review on the recent literature works in the development of different type of ATPSs and their applications in novel separations and purifications of biomaterials. Graphical abstract: The development of Aqueous Two-Phase flotation from Aqueous Two-Phase system

  • Aqueous Two-Phase Flotation for the Recovery of Biomolecules
    Separation & Purification Reviews, 2015
    Co-Authors: Sze Ying Lee, Tau Chuan Ling, Ianatul Khoiroh, Pau Loke Show
    Abstract:

    Aqueous Two-Phase flotation (ATPF), a novel technique which integrates the principles of Aqueous Two-Phase systems and mass transfer mode of solvent sublation, has been successfully applied for the separation and purification of biological materials in the field of biotechnology. From the studies done so far, ATPF has demonstrated as an effective, economical and environment-friendly technique for separation of labile biomolecules from their crude Aqueous extracts of natural plants or fermentation broths, under mild separation condition. In this article, the basic principles, experimental apparatus set up, key design variables of ATPF, ATPF applications and a comparative study between ATPF and other conventional methods are discussed. This review is intended to present a comprehensive summary on the research works carried out so far related to ATPF since its introduction in 2009 until present, which will be helpful for further research in ATPF.

G.s. Luo - One of the best experts on this subject based on the ideXlab platform.

  • Aqueous Two-Phase electrophoresis for separation of amino acids
    Separation and Purification Technology, 2001
    Co-Authors: S.l Zhai, G.s. Luo, J.g Liu
    Abstract:

    Two-Phase electrophoresis, coupling traditional solvent extraction with electrophoresis, is a novel separation technique. Being bio-compatible, Aqueous Two-Phase electrophoresis provides a successful method for separating mixtures of biomolecules. In this work, the separation of amino acids by Aqueous Two-Phase electrophoresis was examined with dextran-polyethylene glycol-water as a working system. The influences of separation time, field strength, initial solute concentration and Two-Phase contact area were studied in intermittent separation equipment. The experiment results show that Aqueous Two-Phase electrophoresis is an effective separation technique for amino acids. With time, field strength, and contact area increasing, mass fluxes across the interface are increased quickly.

  • Selective recovery of amino acids by Aqueous Two-Phase electrophoresis
    Chemical Engineering Journal, 2001
    Co-Authors: S.l Zhai, G.s. Luo, J.g Liu
    Abstract:

    Abstract Aqueous Two-Phase systems (ATPSs) formed by spontaneous Phase separation of different water-soluble polymers are bio-compatible. Aqueous Two-Phase electrophoresis, coupling traditional solvent extraction with electrophoresis, is a novel separation technique and provides a successful method for separating mixtures of biomolecules. In this work, the selective recovery of amino acids by Aqueous Two-Phase electrophoresis was examined with dextran–polyethylene glycol–water as a working system. The experiment results show that glutamic acid can be separated from phenylalanine and tryptophan successfully. The influences of the electrophoresis time, field strength, and Phase volume ratio were studied in an intermittent separation equipment.

Maria-regina Kula - One of the best experts on this subject based on the ideXlab platform.

  • Physical properties of detergent-based Aqueous Two–Phase systems
    Bioseparation, 2001
    Co-Authors: Klaus Selber, Stefan Müller, Holger Gieren, Jörg Thömmes, Thomas Sottmann, Reinhard Strey, Maria-regina Kula
    Abstract:

    The physical behavior of the binary Phase systems of the non-ionic polyoxyethylene detergent Agrimul NRE 1205 and water was investigated. This technical detergent can be used for the large-scale recovery of biomolecules in detergent based Aqueous Two-Phase systems. The Phase diagram was determined. It shows significant and unexpected differences to highly purified detergents. Very similar to neat detergents the Phase diagram can be influenced by auxiliary chemicals thus shifting the entire Phase diagram in general to lower temperatures. This was demonstrated by lowering the cloud-point by various additions. The concentration factor, as an important parameter of a first capture step in purification was investigated and modeled. Auxiliary chemicals, temperature change and change in detergent concentration also influence the viscosity and density of the Phases. These experimental data are shown. They can help to explain the separation behavior of proteins. In large-scale separations Aqueous Two-Phase systems are separated using disc-stack centrifuges. It is demonstrated that this is not a feasible method for detergent-based Aqueous Two-Phase extraction and the physical reason is presented.

  • Physical properties of detergent-based Aqueous Two-Phase systems.
    Bioseparation, 2001
    Co-Authors: Klaus Selber, Stefan Müller, Holger Gieren, Jörg Thömmes, Thomas Sottmann, Reinhard Strey, Maria-regina Kula
    Abstract:

    The physical behavior of the binary Phase systems of the non-ionic polyoxyethylene detergent Agrimul NRE 1205 and water was investigated. This technical detergent can be used for the large-scale recovery of biomolecules in detergent based Aqueous Two-Phase systems. The Phase diagram was determined. It shows significant and unexpected differences to highly purified detergents. Very similar to neat detergents the Phase diagram can be influenced by auxiliary chemicals thus shifting the entire Phase diagram in general to lower temperatures. This was demonstrated by lowering the cloud-point by various additions. The concentration factor, as an important parameter of a first capture step in purification was investigated and modeled. Auxiliary chemicals, temperature change and change in detergent concentration also influence the viscosity and density of the Phases. These experimental data are shown. They can help to explain the separation behavior of proteins. In large-scale separations Aqueous Two-Phase systems are separated using disc-stack centrifuges. It is demonstrated that this is not a feasible method for detergent-based Aqueous Two-Phase extraction and the physical reason is presented.