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Kai Mangelsdorf - One of the best experts on this subject based on the ideXlab platform.
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Methanosarcina spelaei sp. nov., a methanogenic Archaeon isolated from a floating biofilm of a subsurface sulphurous lake.
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Lars Ganzert, Janosch Schirmack, Mashal Alawi, Kai Mangelsdorf, Alexandra Hillebrand-voiculescu, Wolfgang Sand, Dirk WagnerAbstract:A novel methanogenic Archaeon, strain MC-15T, was isolated from a floating biofilm on a sulphurous subsurface lake in Movile Cave (Mangalia, Romania). Cells were non-motile sarcina-like cocci with a diameter of 2–4 µm, occurring in aggregates. The strain was able to grow autotrophically on H2/CO2. Additionally, acetate, methanol, monomethylamine, dimethylamine and trimethylamine were utilized, but not formate or dimethyl sulfide. Trypticase peptone and yeast extract were not required for growth. Optimal growth was observed at 33 °C, pH 6.5 and a salt concentration of 0.05 M NaCl. The predominant membrane lipids of MC-15T were archaeol and hydroxyarchaeol phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol as well as hydroxyarchaeol phosphatidylserine and archaeol glycosaminyl phosphatidylinositol. The closely related species, Methanosarcina vacuolata and Methanosarcina horonobensis, had a similar composition of major membrane lipids to strain MC-15T. The 16S rRNA gene sequence of strain MC-15T was similar to those of Methanosarcina vacuolata DSM 1232T (sequence similarity 99.3 %), Methanosarcina horonobensis HB-1T (98.8 %), Methanosarcina barkeri DSM 800T (98.7 %) and Methanosarcina siciliae T4/MT (98.4 %). DNA–DNA hybridization revealed 43.3 % relatedness between strain MC-15T and Methanosarcina vacuolata DSM 1232T. The G+C content of the genomic DNA was 39.0 mol%. Based on physiological, phenotypic and genotypic differences, strain MC-15T represents a novel species of the genus Methanosarcina , for which the name Methanosarcina spelaei sp. nov. is proposed. The type strain is MC-15T ( = DSM 26047T = JCM 18469T).
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methanosarcina soligelidi sp nov a desiccation and freeze thaw resistant methanogenic Archaeon from a siberian permafrost affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil.
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21(T), was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3-2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21(T) were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053(T) (similarity 99.9 %) and Methanosarcina horonobensis HB-1(T) (similarity 98.7 %). On basis of the level of DNA-DNA hybridization (22.1 %) between strain SMA-21(T) and Methanosarcina mazei DSM 2053(T) as well as of phenotypic and genotypic differences, strain SMA-21(T) was assigned to a novel species of the genus Methanosarcina, for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21(T) (=DSM 26065(T) [corrected] = JCM 18468).
Dirk Wagner - One of the best experts on this subject based on the ideXlab platform.
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Methanosarcina spelaei sp. nov., a methanogenic Archaeon isolated from a floating biofilm of a subsurface sulphurous lake.
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Lars Ganzert, Janosch Schirmack, Mashal Alawi, Kai Mangelsdorf, Alexandra Hillebrand-voiculescu, Wolfgang Sand, Dirk WagnerAbstract:A novel methanogenic Archaeon, strain MC-15T, was isolated from a floating biofilm on a sulphurous subsurface lake in Movile Cave (Mangalia, Romania). Cells were non-motile sarcina-like cocci with a diameter of 2–4 µm, occurring in aggregates. The strain was able to grow autotrophically on H2/CO2. Additionally, acetate, methanol, monomethylamine, dimethylamine and trimethylamine were utilized, but not formate or dimethyl sulfide. Trypticase peptone and yeast extract were not required for growth. Optimal growth was observed at 33 °C, pH 6.5 and a salt concentration of 0.05 M NaCl. The predominant membrane lipids of MC-15T were archaeol and hydroxyarchaeol phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol as well as hydroxyarchaeol phosphatidylserine and archaeol glycosaminyl phosphatidylinositol. The closely related species, Methanosarcina vacuolata and Methanosarcina horonobensis, had a similar composition of major membrane lipids to strain MC-15T. The 16S rRNA gene sequence of strain MC-15T was similar to those of Methanosarcina vacuolata DSM 1232T (sequence similarity 99.3 %), Methanosarcina horonobensis HB-1T (98.8 %), Methanosarcina barkeri DSM 800T (98.7 %) and Methanosarcina siciliae T4/MT (98.4 %). DNA–DNA hybridization revealed 43.3 % relatedness between strain MC-15T and Methanosarcina vacuolata DSM 1232T. The G+C content of the genomic DNA was 39.0 mol%. Based on physiological, phenotypic and genotypic differences, strain MC-15T represents a novel species of the genus Methanosarcina , for which the name Methanosarcina spelaei sp. nov. is proposed. The type strain is MC-15T ( = DSM 26047T = JCM 18469T).
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methanosarcina soligelidi sp nov a desiccation and freeze thaw resistant methanogenic Archaeon from a siberian permafrost affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil.
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21(T), was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3-2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21(T) were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053(T) (similarity 99.9 %) and Methanosarcina horonobensis HB-1(T) (similarity 98.7 %). On basis of the level of DNA-DNA hybridization (22.1 %) between strain SMA-21(T) and Methanosarcina mazei DSM 2053(T) as well as of phenotypic and genotypic differences, strain SMA-21(T) was assigned to a novel species of the genus Methanosarcina, for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21(T) (=DSM 26065(T) [corrected] = JCM 18468).
Lars Ganzert - One of the best experts on this subject based on the ideXlab platform.
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Methanosarcina spelaei sp. nov., a methanogenic Archaeon isolated from a floating biofilm of a subsurface sulphurous lake.
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Lars Ganzert, Janosch Schirmack, Mashal Alawi, Kai Mangelsdorf, Alexandra Hillebrand-voiculescu, Wolfgang Sand, Dirk WagnerAbstract:A novel methanogenic Archaeon, strain MC-15T, was isolated from a floating biofilm on a sulphurous subsurface lake in Movile Cave (Mangalia, Romania). Cells were non-motile sarcina-like cocci with a diameter of 2–4 µm, occurring in aggregates. The strain was able to grow autotrophically on H2/CO2. Additionally, acetate, methanol, monomethylamine, dimethylamine and trimethylamine were utilized, but not formate or dimethyl sulfide. Trypticase peptone and yeast extract were not required for growth. Optimal growth was observed at 33 °C, pH 6.5 and a salt concentration of 0.05 M NaCl. The predominant membrane lipids of MC-15T were archaeol and hydroxyarchaeol phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol as well as hydroxyarchaeol phosphatidylserine and archaeol glycosaminyl phosphatidylinositol. The closely related species, Methanosarcina vacuolata and Methanosarcina horonobensis, had a similar composition of major membrane lipids to strain MC-15T. The 16S rRNA gene sequence of strain MC-15T was similar to those of Methanosarcina vacuolata DSM 1232T (sequence similarity 99.3 %), Methanosarcina horonobensis HB-1T (98.8 %), Methanosarcina barkeri DSM 800T (98.7 %) and Methanosarcina siciliae T4/MT (98.4 %). DNA–DNA hybridization revealed 43.3 % relatedness between strain MC-15T and Methanosarcina vacuolata DSM 1232T. The G+C content of the genomic DNA was 39.0 mol%. Based on physiological, phenotypic and genotypic differences, strain MC-15T represents a novel species of the genus Methanosarcina , for which the name Methanosarcina spelaei sp. nov. is proposed. The type strain is MC-15T ( = DSM 26047T = JCM 18469T).
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methanosarcina soligelidi sp nov a desiccation and freeze thaw resistant methanogenic Archaeon from a siberian permafrost affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil.
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21(T), was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3-2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21(T) were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053(T) (similarity 99.9 %) and Methanosarcina horonobensis HB-1(T) (similarity 98.7 %). On basis of the level of DNA-DNA hybridization (22.1 %) between strain SMA-21(T) and Methanosarcina mazei DSM 2053(T) as well as of phenotypic and genotypic differences, strain SMA-21(T) was assigned to a novel species of the genus Methanosarcina, for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21(T) (=DSM 26065(T) [corrected] = JCM 18468).
Janosch Schirmack - One of the best experts on this subject based on the ideXlab platform.
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Methanosarcina spelaei sp. nov., a methanogenic Archaeon isolated from a floating biofilm of a subsurface sulphurous lake.
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Lars Ganzert, Janosch Schirmack, Mashal Alawi, Kai Mangelsdorf, Alexandra Hillebrand-voiculescu, Wolfgang Sand, Dirk WagnerAbstract:A novel methanogenic Archaeon, strain MC-15T, was isolated from a floating biofilm on a sulphurous subsurface lake in Movile Cave (Mangalia, Romania). Cells were non-motile sarcina-like cocci with a diameter of 2–4 µm, occurring in aggregates. The strain was able to grow autotrophically on H2/CO2. Additionally, acetate, methanol, monomethylamine, dimethylamine and trimethylamine were utilized, but not formate or dimethyl sulfide. Trypticase peptone and yeast extract were not required for growth. Optimal growth was observed at 33 °C, pH 6.5 and a salt concentration of 0.05 M NaCl. The predominant membrane lipids of MC-15T were archaeol and hydroxyarchaeol phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol as well as hydroxyarchaeol phosphatidylserine and archaeol glycosaminyl phosphatidylinositol. The closely related species, Methanosarcina vacuolata and Methanosarcina horonobensis, had a similar composition of major membrane lipids to strain MC-15T. The 16S rRNA gene sequence of strain MC-15T was similar to those of Methanosarcina vacuolata DSM 1232T (sequence similarity 99.3 %), Methanosarcina horonobensis HB-1T (98.8 %), Methanosarcina barkeri DSM 800T (98.7 %) and Methanosarcina siciliae T4/MT (98.4 %). DNA–DNA hybridization revealed 43.3 % relatedness between strain MC-15T and Methanosarcina vacuolata DSM 1232T. The G+C content of the genomic DNA was 39.0 mol%. Based on physiological, phenotypic and genotypic differences, strain MC-15T represents a novel species of the genus Methanosarcina , for which the name Methanosarcina spelaei sp. nov. is proposed. The type strain is MC-15T ( = DSM 26047T = JCM 18469T).
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methanosarcina soligelidi sp nov a desiccation and freeze thaw resistant methanogenic Archaeon from a siberian permafrost affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil.
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21(T), was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3-2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21(T) were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053(T) (similarity 99.9 %) and Methanosarcina horonobensis HB-1(T) (similarity 98.7 %). On basis of the level of DNA-DNA hybridization (22.1 %) between strain SMA-21(T) and Methanosarcina mazei DSM 2053(T) as well as of phenotypic and genotypic differences, strain SMA-21(T) was assigned to a novel species of the genus Methanosarcina, for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21(T) (=DSM 26065(T) [corrected] = JCM 18468).
Daria Morozova - One of the best experts on this subject based on the ideXlab platform.
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methanosarcina soligelidi sp nov a desiccation and freeze thaw resistant methanogenic Archaeon from a siberian permafrost affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21T, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3–2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053T (similarity 99.9 %) and Methanosarcina horonobensis HB-1T (similarity 98.7 %). On basis of the level of DNA–DNA hybridization (22.1 %) between strain SMA-21T and Methanosarcina mazei DSM 2053T as well as of phenotypic and genotypic differences, strain SMA-21T was assigned to a novel species of the genus Methanosarcina , for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21T ( = DSM 20065T = JCM 18468).
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Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic Archaeon from a Siberian permafrost-affected soil.
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Dirk Wagner, Lars Ganzert, Janosch Schirmack, Daria Morozova, Kai MangelsdorfAbstract:A methanogenic Archaeon, strain SMA-21(T), was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3-2.5 µm. Optimal growth was observed at 28 °C, pH 7.8 and 0.02 M NaCl. The strain grew on H2/CO2, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21(T) were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053(T) (similarity 99.9 %) and Methanosarcina horonobensis HB-1(T) (similarity 98.7 %). On basis of the level of DNA-DNA hybridization (22.1 %) between strain SMA-21(T) and Methanosarcina mazei DSM 2053(T) as well as of phenotypic and genotypic differences, strain SMA-21(T) was assigned to a novel species of the genus Methanosarcina, for which the name Methanosarcina soligelidi sp. nov. is proposed. The type strain is SMA-21(T) (=DSM 26065(T) [corrected] = JCM 18468).