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Veronique Seidel - One of the best experts on this subject based on the ideXlab platform.

  • antitubercular activity of Arctium lappa and tussilago farfara extracts and constituents
    Journal of Ethnopharmacology, 2014
    Co-Authors: Jinlian Zhao, Dimitrios Evangelopoulos, Sanjib Bhakta, Alexander I Gray, Veronique Seidel
    Abstract:

    Abstract Ethnopharmacological relevance Arctium lappa and Tussilago farfara (Asteraceae) are two plant species used traditionally as antitubercular remedies. The aim of this study was (i) to screen Arctium lappa and Tussilago farfara extracts for activity against Mycobacterium tuberculosis and (ii) to isolate and identify the compound(s) responsible for this reputed anti-TB effect. Materials and methods The activity of extracts and isolated compounds was determined against Mycobacterium tuberculosis H37Rv using a high throughput spot culture growth inhibition (HT-SPOTi) assay. Results The n-hexane extracts of both plants, the ethyl acetate extract of Tussilago farfara and the dichloromethane phase derived from the methanol extract of Arctium lappa displayed antitubercular activity (MIC 62.5 μg/mL). Further chemical investigation of Arctium lappa led to the isolation of n-nonacosane (1), taraxasterol acetate (2), taraxasterol (3), a (1:1) mixture of β sitosterol/stigmasterol (4), isololiolide (5), melitensin (6), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10). Compounds isolated from Tussilago farfara were identified as a (1:1) mixture of β sitosterol/stigmasterol (4), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10), loliolide (11), a (4:1) mixture of p-coumaric acid/4-hydroxybenzoic acid (12), p-coumaric acid (13). All compounds were identified following analyses of their physicochemical and spectroscopic data (MS, 1H and 13C-NMR) and by comparison with published data. This is the first report of the isolation of n-nonacosane (1), isololiolide (5), melitensin (6) and kaempferol-3-O-glucoside (10) from Arctium lappa, and of loliolide (11) from Tussilago farfara. Amongst the isolated compounds, the best activity was observed for p-coumaric acid (13) (MIC 31.3 μg/mL or 190.9 μM) alone and in mixture with 4-hydroxybenzoic acid (12) (MIC 62.5 μg/mL). Conclusions The above results provide for the first time some scientific evidence to support, to some extent, the ethno-medicinal use of Arctium lappa and Tussilago farfara as traditional antitubercular remedies.

Chen Kao-shan - One of the best experts on this subject based on the ideXlab platform.

  • Effect of aqueous extract of Arctium lappa L. (burdock) roots on the sexual behavior of male rats
    BMC complementary and alternative medicine, 2012
    Co-Authors: Cao Jianfeng, Zhang Pengying, Xu Chengwei, Huang Taotao, Bai Yungui, Chen Kao-shan
    Abstract:

    Arctium lappa L. root has traditionally been recommended as an aphrodisiac agent. It is used to treat impotence and sterility in China, and Native Americans included the root in herbal preparations for women in labor. However, its use has not been scientifically validated. The present study therefore investigated the effects of aqueous extract of Arctium lappa L. roots on sexual behavior in normal male rats. Seventy-five albino male rats were randomly divided into five groups of 15 rats each. Rats in group 1 (control) were administered 10 mL⁄kg body weight distilled water (vehicle), group 2 received 60 mg/kg body weight sildenafil citrate (Viagra), while those in groups 3, 4, and 5 were given 300, 600, and 1,200 mg/kg body weight, respectively, of aqueous extract of Arctium lappa L. roots in the same volume. Female albino rats were made receptive by hormonal treatment. Sexual behavior parameters in male rats were monitored on days 3, 7 and 15 by pairing with receptive females (1:3). Male serum testosterone concentrations and potency were also determined. Oral administration of Arctium lappa L. roots extract at 600 and 1,200 mg/kg body weight significantly increased the frequencies of mount, intromission, and ejaculation frequency (p < 0.05). The latencies of mount and intromission were significantly reduced and ejaculation latency was prolonged. Administration of the extract also reduced the post-ejaculatory interval. The standard drug (Viagra) was more effective than the extract. The extract significantly increased the frequencies of all components of penile reflexes as well as serum testosterone levels, compared with the distilled water controls. The results of this study demonstrate that aqueous extract of Arctium lappa L. roots enhances sexual behavior in male rats. The aphrodisiac effects of the plant extract may be related to the presence of flavonoids, saponins, lignans and alkaloids, acting via a multitude of central and peripheral mechanisms. These results thus support the traditional use of Arctium lappa L. root extract for treating impotence and sterility.

  • GC-MS analysis of volatile oil from slices of Arctium lappa L.by supercritical CO_2 extraction
    2012
    Co-Authors: Chen Kao-shan
    Abstract:

    Objective: To verify the feasibility of obtaining the volatile oil from slices of Arctium lappa L.by supercritical carbon dioxide(CO2) extraction and determine the chemical components of the extract.Methods: The volatile oil was obtained by supercritical CO2 and the chemical components were isolated and identified by gas chromatography-mass spectrometry(GC-MS).Results: The extraction rate was 0.84 %,and 31 compounds were identified from 41 extraction peaks.Conclusion: The technology is competent for extracting the volatile oil from slices of Arctium lappa L.by supercritical CO2,and the oil was identified as a mixture of multiple chemical components.

  • Chemical Constituents of the Root of Arctium lappa L.
    Natural Product Research and Development, 2011
    Co-Authors: Chen Kao-shan
    Abstract:

    Six compounds were isolated and purified from 95% ethanol extract of the root of Arctium lappa L.by chromatographic methods.Among them,five chemical constituents were identified by physico-chemical and spectroscopic analysis as arctiin(1),kaempferol(2),adenosine(3),solasonine(4),and spirosl-3-O-α-L-rhamnopyrannosyl-(1→4)-O-β-D-galactopyranosyl(5).Compounds 2～5 were firstly isolated from the burdock root.

  • Optimal extracting processes of chlorogenic acid from Arctium lappa
    The Chinese Pharmaceutical Journal, 2003
    Co-Authors: Chen Kao-shan
    Abstract:

    OBJECTIVE To optimize the extracting processes of chlorogenic acid from Arctium lappa L.leaves.METHODS The extraction of chlorogenic acid in Arctium lappa L.leaves was investigated with the changes of temperature,pH of extracting solutions,extracting time and frequency and Arctium lappa L.leaves-treated methods.The contents of chlorogenic acid were measured by means of RP-HPLC.RESULTS Either methanol (pH 2～3) at 65℃ or ethanol (pH 2～3) at 80℃ was beneficial for extracting chlorogenic acid.The better extracting time was 4 h,which included 2 h for each time or 2 h for the 1 st time and 1 h for the 2 nd and 3 rd time respectively.CONCLUSION The better extracting processes should be as following:taking Arctium lappa L.leaves dried at normal temperature as the raw materials,extracting at 65℃ with methanol or 80℃ with ethanol,pH=2～3,for 4 h which included 2 h for each time or 2 h for the 1st time and 1 h for the 2nd and 3rd time respectively.

Jinlian Zhao - One of the best experts on this subject based on the ideXlab platform.

  • antitubercular activity of Arctium lappa and tussilago farfara extracts and constituents
    Journal of Ethnopharmacology, 2014
    Co-Authors: Jinlian Zhao, Dimitrios Evangelopoulos, Sanjib Bhakta, Alexander I Gray, Veronique Seidel
    Abstract:

    Abstract Ethnopharmacological relevance Arctium lappa and Tussilago farfara (Asteraceae) are two plant species used traditionally as antitubercular remedies. The aim of this study was (i) to screen Arctium lappa and Tussilago farfara extracts for activity against Mycobacterium tuberculosis and (ii) to isolate and identify the compound(s) responsible for this reputed anti-TB effect. Materials and methods The activity of extracts and isolated compounds was determined against Mycobacterium tuberculosis H37Rv using a high throughput spot culture growth inhibition (HT-SPOTi) assay. Results The n-hexane extracts of both plants, the ethyl acetate extract of Tussilago farfara and the dichloromethane phase derived from the methanol extract of Arctium lappa displayed antitubercular activity (MIC 62.5 μg/mL). Further chemical investigation of Arctium lappa led to the isolation of n-nonacosane (1), taraxasterol acetate (2), taraxasterol (3), a (1:1) mixture of β sitosterol/stigmasterol (4), isololiolide (5), melitensin (6), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10). Compounds isolated from Tussilago farfara were identified as a (1:1) mixture of β sitosterol/stigmasterol (4), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10), loliolide (11), a (4:1) mixture of p-coumaric acid/4-hydroxybenzoic acid (12), p-coumaric acid (13). All compounds were identified following analyses of their physicochemical and spectroscopic data (MS, 1H and 13C-NMR) and by comparison with published data. This is the first report of the isolation of n-nonacosane (1), isololiolide (5), melitensin (6) and kaempferol-3-O-glucoside (10) from Arctium lappa, and of loliolide (11) from Tussilago farfara. Amongst the isolated compounds, the best activity was observed for p-coumaric acid (13) (MIC 31.3 μg/mL or 190.9 μM) alone and in mixture with 4-hydroxybenzoic acid (12) (MIC 62.5 μg/mL). Conclusions The above results provide for the first time some scientific evidence to support, to some extent, the ethno-medicinal use of Arctium lappa and Tussilago farfara as traditional antitubercular remedies.

Man Ki Park - One of the best experts on this subject based on the ideXlab platform.

Ho Sub Lee - One of the best experts on this subject based on the ideXlab platform.