The Experts below are selected from a list of 28395 Experts worldwide ranked by ideXlab platform
Gea Guerriero - One of the best experts on this subject based on the ideXlab platform.
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insights into Lignan composition and biosynthesis in stinging nettle urtica dioica l
Molecules, 2019Co-Authors: Xuan Xu, Cedric Guignard, Jenny Renaut, Jean-francois Hausman, Edoardo Gatti, Stefano Predieri, Gea GuerrieroAbstract:Stinging nettle (Urtica dioica L.) has been used as herbal medicine to treat various ailments since ancient times. The biological activity of nettle is chiefly attributed to a large group of phenylpropanoid dimers, namely Lignans. Despite the pharmacological importance of nettle Lignans, there are no studies addressing Lignan biosynthesis in this plant. We herein identified 14 genes encoding dirigent proteins (UdDIRs) and 3 pinoresinol-lariciresinol reductase genes (UdPLRs) in nettle, which are two gene families known to be associated with Lignan biosynthesis. Expression profiling of these genes on different organs/tissues revealed a specific expression pattern. Particularly, UdDIR7, 12 and 13 displayed a remarkable high expression in the top internode, fibre tissues of bottom internodes and roots, respectively. The relatively high expression of UdPLR1 and UdPLR2 in the young internodes, core tissue of bottom internode and roots is consistent with the high accumulation of lariciresinol and secoisolariciresinol in these tissues. Lignan quantification showed a high abundance of pinoresinol in roots and pinoresinol diglucosides in young internodes and leaves. This study sheds light on Lignan composition and biosynthesis in nettle, providing a good basis for further functional analysis of DIRs and PLRs and, ultimately, engineering Lignan metabolism in planta and in cell cultures.
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Insights into the molecular regulation of monolignol-derived product biosynthesis in the growing hemp hypocotyl
BMC Plant Biology, 2018Co-Authors: Marc Behr, Cedric Guignard, Jenny Renaut, Jean-francois Hausman, Kjell Sergeant, Céline C. Leclercq, Sébastien Planchon, Audrey Lenouvel, Stanley Lutts, Gea GuerrieroAbstract:Lignin and Lignans are both derived from the monolignol pathway. Despite the similarity of their building blocks, they fulfil different functions in planta. Lignin strengthens the tissues of the plant, while Lignans are involved in plant defence and growth regulation. Their biosyntheses are tuned both spatially and temporally to suit the development of the plant (water conduction, reaction to stresses). We propose to study the general molecular events related to monolignol-derived product biosynthesis, especially lignin. It was previously shown that the growing hemp hypocotyl (between 6 and 20 days after sowing) is a valid system to study secondary growth and the molecular events accompanying lignification. The present work confirms the validity of this system, by using it to study the regulation of lignin and Lignan biosynthesis. Microscopic observations, lignin analysis, proteomics, together with in situ laccase and peroxidase activity assays were carried out to understand the dynamics of lignin synthesis during the development of the hemp hypocotyl. Based on phylogenetic analysis and targeted gene expression, we suggest a role for the hemp dirigent and dirigent-like proteins in Lignan biosynthesis. The transdisciplinary approach adopted resulted in the gene- and protein-level quantification of the main enzymes involved in the biosynthesis of monolignols and their oxidative coupling (laccases and class III peroxidases), in lignin deposition (dirigent-like proteins) and in the determination of the stereoconformation of Lignans (dirigent proteins). Our work sheds light on how, in the growing hemp hypocotyl, the provision of the precursors needed to synthesize the aromatic biomolecules lignin and Lignans is regulated at the transcriptional and proteomic level.
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Insights into the molecular regulation of monolignol-derived product biosynthesis in the growing hemp hypocotyl
BMC Plant Biology, 2018Co-Authors: Marc Behr, Cedric Guignard, Jenny Renaut, Jean-francois Hausman, Kjell Sergeant, Céline C. Leclercq, Sébastien Planchon, Audrey Lenouvel, Stanley Lutts, Gea GuerrieroAbstract:Background Lignin and Lignans are both derived from the monolignol pathway. Despite the similarity of their building blocks, they fulfil different functions in planta . Lignin strengthens the tissues of the plant, while Lignans are involved in plant defence and growth regulation. Their biosyntheses are tuned both spatially and temporally to suit the development of the plant (water conduction, reaction to stresses). We propose to study the general molecular events related to monolignol-derived product biosynthesis, especially lignin. It was previously shown that the growing hemp hypocotyl (between 6 and 20 days after sowing) is a valid system to study secondary growth and the molecular events accompanying lignification. The present work confirms the validity of this system, by using it to study the regulation of lignin and Lignan biosynthesis. Microscopic observations, lignin analysis, proteomics, together with in situ laccase and peroxidase activity assays were carried out to understand the dynamics of lignin synthesis during the development of the hemp hypocotyl. Results Based on phylogenetic analysis and targeted gene expression, we suggest a role for the hemp dirigent and dirigent-like proteins in Lignan biosynthesis. The transdisciplinary approach adopted resulted in the gene- and protein-level quantification of the main enzymes involved in the biosynthesis of monolignols and their oxidative coupling (laccases and class III peroxidases), in lignin deposition (dirigent-like proteins) and in the determination of the stereoconformation of Lignans (dirigent proteins). Conclusions Our work sheds light on how, in the growing hemp hypocotyl, the provision of the precursors needed to synthesize the aromatic biomolecules lignin and Lignans is regulated at the transcriptional and proteomic level.
D. Trebouet - One of the best experts on this subject based on the ideXlab platform.
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recovery of lignin and Lignans enriched fractions from thermomechanical pulp mill process water through membrane separation technology pilot plant study and techno economic assessment
Journal of Cleaner Production, 2020Co-Authors: Maud Villaingambier, M. Courbalay, A. Klem, S. Dumarçay, D. TrebouetAbstract:Abstract Membrane process is a viable option for valuable compounds such as lignin and Lignans recovery in aqueous discharges of thermomechanical pulp industries. Recovery of lignin and Lignans with continuous mode ultrafiltration/nanofiltration installation has not been studied in detail previously. After flotation used to remove lipophilic matter of pulp mill effluent, the three steps membrane process was composed of a clarification step by a 150 kDa ultrafiltration used to remove suspended matter, followed by two nanofiltrations of 1 kDa and 300 Da to respectively retain lignin and Lignans. A techno-economic evaluation of this process has been performed. The process with a plant capacity of 60 m3 h−1 was able to produce 12 tons of lignin and 825 kg of Lignans per month. The influence of plant capacity between 0.1 and 60 m3 h−1 on extracts production cost allows determining a production cost equal or below 10 €.kg−1 as plant capacity reached 10 m3 h−1. Valorization of lignin and Lignans as parts of the formulas of high added value products could allow thermomechanical plants to head towards biorefinery and cleaner production concepts. Furthemore, the 300 Da permeate possessed the required characteristics to be re used and save fresh water utilization.
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Recovery of lignin and Lignans enriched fractions from thermomechanical pulp mill process water through membrane separation technology: Pilot-plant study and techno-economic assessment
Journal of Cleaner Production, 2020Co-Authors: M. Villain-gambier, M. Courbalay, A. Klem, S. Dumarçay, D. TrebouetAbstract:Membrane process is a viable option for valuable compounds such as lignin and Lignans recovery in aqueous discharges of thermomechanical pulp industries. Recovery of lignin and Lignans with continuous mode ultrafiltration/nanofiltration installation has not been studied in detail previously. After flotation used to remove lipophilic matter of pulp mill effluent, the three steps membrane process was composed of a clarification step by a 150 kDa ultrafiltration used to remove suspended matter, followed by two nanofiltrations of 1 kDa and 300 Da to respectively retain lignin and Lignans. A techno-economic evaluation of this process has been performed. The process with a plant capacity of 60 m(3) h(-1) was able to produce 12 tons of lignin and 825 kg of Lignans per month. The influence of plant capacity between 0.1 and 60 m(3) h(-1) on extracts production cost allows determining a production cost equal or below 10 V.kg(-1) as plant capacity reached 10 m(3) h(-1). Valorization of lignin and Lignans as parts of the formulas of high added value products could allow thermomechanical plants to head towards biorefinery and cleaner production concepts. Furthemore, the 300 Da permeate possessed the required characteristics to be re used and save fresh water utilization. (C) 2019 Elsevier Ltd. All rights reserved.
M. Courbalay - One of the best experts on this subject based on the ideXlab platform.
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recovery of lignin and Lignans enriched fractions from thermomechanical pulp mill process water through membrane separation technology pilot plant study and techno economic assessment
Journal of Cleaner Production, 2020Co-Authors: Maud Villaingambier, M. Courbalay, A. Klem, S. Dumarçay, D. TrebouetAbstract:Abstract Membrane process is a viable option for valuable compounds such as lignin and Lignans recovery in aqueous discharges of thermomechanical pulp industries. Recovery of lignin and Lignans with continuous mode ultrafiltration/nanofiltration installation has not been studied in detail previously. After flotation used to remove lipophilic matter of pulp mill effluent, the three steps membrane process was composed of a clarification step by a 150 kDa ultrafiltration used to remove suspended matter, followed by two nanofiltrations of 1 kDa and 300 Da to respectively retain lignin and Lignans. A techno-economic evaluation of this process has been performed. The process with a plant capacity of 60 m3 h−1 was able to produce 12 tons of lignin and 825 kg of Lignans per month. The influence of plant capacity between 0.1 and 60 m3 h−1 on extracts production cost allows determining a production cost equal or below 10 €.kg−1 as plant capacity reached 10 m3 h−1. Valorization of lignin and Lignans as parts of the formulas of high added value products could allow thermomechanical plants to head towards biorefinery and cleaner production concepts. Furthemore, the 300 Da permeate possessed the required characteristics to be re used and save fresh water utilization.
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Recovery of lignin and Lignans enriched fractions from thermomechanical pulp mill process water through membrane separation technology: Pilot-plant study and techno-economic assessment
Journal of Cleaner Production, 2020Co-Authors: M. Villain-gambier, M. Courbalay, A. Klem, S. Dumarçay, D. TrebouetAbstract:Membrane process is a viable option for valuable compounds such as lignin and Lignans recovery in aqueous discharges of thermomechanical pulp industries. Recovery of lignin and Lignans with continuous mode ultrafiltration/nanofiltration installation has not been studied in detail previously. After flotation used to remove lipophilic matter of pulp mill effluent, the three steps membrane process was composed of a clarification step by a 150 kDa ultrafiltration used to remove suspended matter, followed by two nanofiltrations of 1 kDa and 300 Da to respectively retain lignin and Lignans. A techno-economic evaluation of this process has been performed. The process with a plant capacity of 60 m(3) h(-1) was able to produce 12 tons of lignin and 825 kg of Lignans per month. The influence of plant capacity between 0.1 and 60 m(3) h(-1) on extracts production cost allows determining a production cost equal or below 10 V.kg(-1) as plant capacity reached 10 m(3) h(-1). Valorization of lignin and Lignans as parts of the formulas of high added value products could allow thermomechanical plants to head towards biorefinery and cleaner production concepts. Furthemore, the 300 Da permeate possessed the required characteristics to be re used and save fresh water utilization. (C) 2019 Elsevier Ltd. All rights reserved.
Honoo Satake - One of the best experts on this subject based on the ideXlab platform.
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Lignan Biosynthesis for Food Bioengineering
Food Biosynthesis, 2017Co-Authors: Honoo Satake, Kinuyo Morimoto, Eiichiro Ono, Akira Shiraishi, Tomotsugu Koyama, Erika Matsumoto, Sedigheh Esmaeilzadeh Bahabadi, Jun MurataAbstract:Abstract Functional supplements from dietary or medicinal plant sources are expected to contribute a great deal to extension of health expectancy and improvement of quality of life in the emerging elderly society. Lignans are structurally and functionally diverse phytochemicals and have been widely investigated for the development of healthy diets as well as clinical agents. Recent studies have revealed multiple novel Lignan-biosynthetic pathways, the genomes and transcriptomes of Lignan-rich plants, and exogenous factors for Lignan biosynthesis, which is expected to lead to the efficient, sustainable, and stable production of Lignans in plants. This chapter presents the essences of biosynthetic pathways and biological activities of Lignans, genomes, and transcriptomes of typical Lignan-rich plants, the latest metabolic engineering of Lignan biosynthesis, and the perspectives in the metabolic engineering-based production of dietary Lignans.
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Essences in metabolic engineering of Lignan biosynthesis.
Metabolites, 2015Co-Authors: Honoo Satake, Eiichiro Ono, Tomotsugu Koyama, Erika Matsumoto, Sedigheh Esmaeilzadeh Bahabadi, Jun MurataAbstract:Lignans are structurally and functionally diverse phytochemicals biosynthesized in diverse plant species and have received wide attentions as leading compounds of novel drugs for tumor treatment and healthy diets to reduce of the risks of lifestyle-related non-communicable diseases. However, the lineage-specific distribution and the low-amount of production in natural plants, some of which are endangered species, hinder the efficient and stable production of beneficial Lignans. Accordingly, the development of new procedures for Lignan production is of keen interest. Recent marked advances in the molecular and functional characterization of Lignan biosynthetic enzymes and endogenous and exogenous factors for Lignan biosynthesis have suggested new methods for the metabolic engineering of Lignan biosynthesis cascades leading to the efficient, sustainable, and stable Lignan production in plants, including plant cell/organ cultures. Optimization of light conditions, utilization of a wide range of elicitor treatments, and construction of transiently gene-transfected or transgenic Lignan-biosynthesizing plants are mainly being attempted. This review will present the basic and latest knowledge regarding metabolic engineering of Lignans based on their biosynthetic pathways and biological activities, and the perspectives in Lignan production via metabolic engineering.
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Seasonal Alteration in Amounts of Lignans and Their Glucosides and Gene Expression of the Relevant Biosynthetic Enzymes in the Forsythia suspense Leaf
Biological & pharmaceutical bulletin, 2013Co-Authors: Kinuyo Morimoto, Honoo SatakeAbstract:Lignans of Forsythia spp. are essential components of various Chinese medicines and health diets. However, the seasonal alteration in Lignan amounts and the gene expression profile of Lignan-biosynthetic enzymes has yet to be investigated. In this study, we have assessed seasonal alteration in amounts of major Lignans, such as pinoresinol, matairesinol, and arctigenin, and examined the gene expression profile of pinoresinol/lariciresinol reductase (PLR), pinoresinol-glucosylating enzyme (UGT71A18), and secoisolariciresinol dehydrogenase (SIRD) in the leaf of Forsythia suspense from April to November. All of the Lignans in the leaf continuously increased from April to June, reached the maximal level in June, and then decreased. Ninety percent of pinoresinol and matairesinol was converted into glucosides, while approximately 50% of arctigenin was aglycone. PLR was stably expressed from April to August, whereas the PLR expression was not detected from September to November. In contrast, the UGT71A18 expression was found from August to November, but not from April to July. The SIRD expression was prominent from April to May, not detected in June to July, and then increased again from September to November. These expression profiles of the Lignan-synthetic enzymes are largely compatible with the alteration in Lignan contents. Furthermore, such seasonal Lignan profiles are in good agreement with the fact that the Forsythia leaves for Chinese medicinal tea are harvested in June. This is the first report on seasonal alteration in Lignans and the relevant biosynthetic enzyme genes in the leaf of Forsythia species.
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recent advances in the metabolic engineering of Lignan biosynthesis pathways for the production of transgenic plant based foods and supplements
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Honoo Satake, Eiichiro Ono, Jun MurataAbstract:Plant physiological, epidemiological, and food science studies have shed light on Lignans as healthy diets for the reduction of the risk of lifestyle-related noncommunicable diseases and, thus, the demand for Lignans has been rapidly increasing. However, the low efficiency and instability of Lignan production via extraction from plant resources remain to be resolved, indicating the requirement for the development of new procedures for Lignan production. The metabolic engineering of Lignan-biosynthesizing plants is expected to be most promising for efficient, sustainable, and stable Lignan production. This is supported by the recent verification of biosynthetic pathways of major dietary Lignans and the exploration of Lignan production via metabolic engineering using transiently gene-transfected or transgenic plants. The aim of this review is to present an overview of the biosynthetic pathways, biological activities, and metabolic engineering of Lignans and also perspectives in metabolic engineering-based l...
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Metabolic Engineering of Lignan Biosynthesis in Forsythia Cell Culture
Plant & cell physiology, 2009Co-Authors: Hyun-jung Kim, Kinuyo Morimoto, Eiichiro Ono, Atsushi Okazawa, Akio Kobayashi, Tohru Yamagaki, Honoo SatakeAbstract:Lignans are a large class of secondary metabolites in plants, with numerous biological effects in mammals, including antitumor and antioxidant activities. Sesamin, the most abundant furofuran-class Lignan in sesame seeds (Sesamum plants), is produced by the cytochrome P450 enzyme CYP81Q1 from the precursor Lignan, pinoresinol. In contrast, Forsythia plants produce dibenzylbutyrolactone-class Lignans, such as matairesinol, from pinoresinol via the catalysis of pinoresinol/lariciresinol reductase (PLR) and secoisolariciresinol dehydrogenase. Here we present the engineering of Lignan biosynthesis in Forsythia cell suspension cultures for the development of an efficient production method of beneficial Lignans. A suspension cell culture prepared from leaves of Forsythia koreana produced Lignans, mainly pinoresinol and matairesinol glucosides, at levels comparable with that obtained from the leaves. In an attempt to increase the pinoresinol content in Forsythia, we generated a transgenic cell line overexpressing an RNA interference (RNAi) construct of PLR (PLR-RNAi). Down-regulation of PLR expression led to a complete loss of matairesinol and an accumulation of approximately 20-fold pinoresinol in its glucoside form in comparison with the non-transformant. Moreover, the Forsythia transgenic cells co-expressing CYP81Q1 and PLR-RNAi exhibited production of sesamin as well as accumulation of pinoresinol glucoside. These data suggest Forsythia cell suspension to be a promising tool for the engineering of Lignan production. To the best of our knowledge, this is the first report on transgenic production of an exogenous Lignan in a plant species.
Bjarne Holmbom - One of the best experts on this subject based on the ideXlab platform.
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Variation of Lignans in Norway spruce (Picea abies [L.] Karst.) knotwood: within-stem variation and the effect of fertilisation at two experimental sites in Finland
Trees, 2008Co-Authors: Riikka Piispanen, Stefan Willför, Pekka Saranpää, Bjarne HolmbomAbstract:Lignan concentrations in Norway spruce ( Picea abies [L.] Karst.) knotwood were studied in two long-term nitrogen fertilised experimental sites: a boreal site (66°5′N, 27°07′E) close to the Arctic tree line in northern Finland and a boreal site (61°10′N, 26°03′E) in southern Finland. Altogether 20 mature trees, representing five different size classes, were felled in the autumn 2002 and knots of the largest and smallest diameter in each whorl at the height of 4 m were analysed. Within-stem variation of knotwood Lignans was studied at the 4 m intervals from stump height to the top from five control trees in the southern site. The following knotwood Lignans were identified with GC-MS: hydroxymatairesinol (two isomers), secoisolariciresinol, α-conidendrin, α-conidendric acid, isohydroxymatairesinol, lariciresinol, Lignan A, matairesinol, nortrachelogenin, todolactol A and isoliovil. The predominant Lignan in Norway spruce knotwood was hydroxymatairesinol comprising approximately 77% of the total concentration of Lignans. In mature trees in the southern site, the Lignan concentrations were highest close to the starting point of the living branches. In the northern site, the total concentration of knotwood Lignans was significantly higher (approximately 14% of the dry mass) than in the southern site (approximately 5.4% of the dry mass). In the northern site in control trees, a negative linear correlation was detected between branch diameter and hydroxymatairesinol concentration, but the similar correlation was not detected in fertilised trees or in the trees of the southern site. The possible reasons for higher hydroxymatairesinol concentrations in the northern knots were discussed.
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quantification of a broad spectrum of Lignans in cereals oilseeds and nuts
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Annika Smeds, Takeshi Deyama, Stefan Willför, Rainer Sjöholm, Patrik Eklund, Sansei Nishibe, Bjarne HolmbomAbstract:Twenty-four plant Lignans were analyzed by high-performance liquid chromatography−tandem mass spectrometry in bran extracts of 16 cereal species, in four nut species, and in two oilseed species (sesame seeds and linseeds). Eighteen of these were Lignans previously unidentified in these species, and of these, 16 were identified in the analyzed samples. Four different extraction methods were applied as follows: alkaline extraction, mild acid extraction, a combination of alkaline and mild acid extraction, or accelerated solvent extraction. The extraction method was of great importance for the Lignan yield. 7-Hydroxymatairesinol, which has not previously been detected in cereals because of destructive extraction methods, was the dominant Lignan in wheat, triticale, oat, barley, millet, corn bran, and amaranth whole grain. Syringaresinol was the other dominant cereal Lignan. Wheat and rye bran had the highest Lignan content of all cereals; however, linseeds and sesame seeds were by far the most Lignan-rich of...
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Knots in trees - A new rich source of Lignans
Phytochemistry Reviews, 2003Co-Authors: Bjarne Holmbom, Rainer Sjöholm, Patrik Eklund, Christer Eckerman, Jarl Hemming, Linda Nisula, Markku Reunanen, Anna Sundberg, Kenneth Sundberg, Stefan WillförAbstract:Recent research in our group has revealed that knots, i.e. the branch bases inside tree stems, commonly contain 5–10% (w/w) of Lignans. Norway spruce (Picea abies) knots contain as much as 6–24% of Lignans, with 7-hydroxymatairesinol (HMR) as the predominant (70–85%) Lignan. Some other spruce species also contain HMR as the main Lignan, but some spruce species have also other dominating Lignans. Most fir (Abies) species contain secoisolariciresinol and lariciresinol as the main Lignans. Lignans occur also in knots of pines (Pinus spp.), although in lower amounts than in spruces and firs. Scots pine (Pinus silvestris) knots were found to contain 0.4–3% of Lignans with nortrachelogenin as the main Lignan. Lignans have been identified also in knots of some hardwoods, although flavonoids are more abundant in hardwoods. Knots are detrimental in the manufacture of pulp and paper and should preferably be removed before pulping. This is possible using a recently developed industrially applicable process called ChipSep. Recent research has also established novel synthetic routes to several Lignans, such as matairesinol, secoisolariciresinol, lariciresinol and cyclolariciresinol, starting from hydroxymatairesinol by applying fairly straight-forward chemical transformations. We conclude that wood knots in certain spruce and fir species constitute the richest known source of Lignans in nature. The Lignans occur in knots in free form and are easily extracted by aqueous ethanol, or even by water. Not only HMR, but also other potentially valuable Lignans, could be produced in a scale of hundreds of tons per year by extraction of knots separated from wood chips at pulp and paper mills.
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Modification of Spruce Lignans with Trametes hirsuta Laccase
Holzforschung, 2002Co-Authors: Johanna Buchert, Tarja Tamminen, Annikka Mustranta, Peter Spetz, Bjarne HolmbomAbstract:The effect of Trametes hirsuta laccase on isolated spruce wood Lignans was evaluated. Lignans were isolated from the heartwood of spruce branches and treated with different laccase dosages and treatment times. The effect of the treatment was monitored by gas chromatography, size exclusion chromatography and ionization difference UV spectroscopy. Lignans were efficiently oxidized by T. hirsuta laccase. About half of the phenolic groups present in Lignans remained intact during the treatment. The oxidation of phenolic groups in Lignans produced oligomeric structures containing approximately 4-5 Lignan units (i.e., 8-10 phenyl propane units). Precipitation of the formed oligomeric structures probably prevented further polymerization.
