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Brigitte Kopp - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of glucocerebrosides and the active metabolite 4,8-sphingadienine from Arisaema amurense and Pinellia ternata by NMR and CD spectroscopy and ESI-MS/CID-MS.
    Journal of Agricultural and Food Chemistry, 2012
    Co-Authors: E. Rozema, Ruxandra Popescu, Harald Sonderegger, Christian W. Huck, Johannes Winkler, Georg Krupitza, Ernst Urban, Brigitte Kopp
    Abstract:

    Sphingolipid metabolites regulate cellular processes such as cell proliferation, differentiation, and apoptosis. In this study, glucocerebrosides (GluCer) from rhizomes of Arisaema amurense and Pinellia ternata were fully characterized using 1- and 2-dimensional nuclear magnetic spin resonance (NMR) and circular dichroism (CD) spectroscopy and tandem collision-induced dissociation mass spectrometry (ESI-MS/CID-MS). Three new acylated and seven known GluCer were elucidated with 4,8-sphingadienine (4,8-SD, d18:2) as backbone. 4,8-SD is a metabolite after enzymatical hydrolysis of GluCer in the gut lumen. In this study, 4,8-SD was hydrolyzed from GluCer and chromatographically purified on silica gel. In contrast to the GluCer, 4,8-SD showed cytotoxic effects in the WST-1 assay. GluCer with 4,8-SD as sphingoid backbone are present in plants consumed as food, such as spinach, soy, and eggplant.

  • Characterization of Glucocerebrosides and the Active Metabolite 4,8-Sphingadienine from Arisaema amurense and Pinellia ternata by NMR and CD Spectroscopy and ESI-MS/CID-MS
    2012
    Co-Authors: E. Rozema, Ruxandra Popescu, Harald Sonderegger, Johannes Winkler, Georg Krupitza, Ernst Urban, Christian W. Huck, Brigitte Kopp
    Abstract:

    Sphingolipid metabolites regulate cellular processes such as cell proliferation, differentiation, and apoptosis. In this study, glucocerebrosides (GluCer) from rhizomes of Arisaema amurense and Pinellia ternata were fully characterized using 1- and 2-dimensional nuclear magnetic spin resonance (NMR) and circular dichroism (CD) spectroscopy and tandem collision-induced dissociation mass spectrometry (ESI-MS/CID-MS). Three new acylated and seven known GluCer were elucidated with 4,8-sphingadienine (4,8-SD, d18:2) as backbone. 4,8-SD is a metabolite after enzymatical hydrolysis of GluCer in the gut lumen. In this study, 4,8-SD was hydrolyzed from GluCer and chromatographically purified on silica gel. In contrast to the GluCer, 4,8-SD showed cytotoxic effects in the WST-1 assay. GluCer with 4,8-SD as sphingoid backbone are present in plants consumed as food, such as spinach, soy, and eggplant

Jin Murata - One of the best experts on this subject based on the ideXlab platform.

  • an updated genus wide phylogenetic analysis of Arisaema araceae with reference to sections
    Botanical Journal of the Linnean Society, 2016
    Co-Authors: Tetsuo Ohitoma, Hiroko Murata, Jin Murata
    Abstract:

    Arisaema has a long and complicated taxonomic history regarding its infrageneric classification. In the latest system, 14 sections were recognized, based on an unpublished, tentative phylogenetic analysis; in addition the type species and nomenclatural priority for each section were confirmed. Here, we present an updated, genus-wide phylogenetic analysis, based on four plastid non-coding regions (3′trnL–trnF, rpl20–5′rps12, psbB–psbH and rpoC2–rps2) for > 150 accessions. The maximum parsimony and maximum likelihood phylogenetic analyses identified eight major clades and one branch with unique sequence variation, although the relationships were unclear due to a polytomy and weak support. In the phylogenetic trees, most of the sections proposed in the latest system were distinct and corresponded to the major clades, but some sections are not monophyletic. On the basis of the phylogenetic relationship: (1) A. schimperianum is treated as a member of section Arisaema, rather than section Tenuipistillata or section SinArisaema; and (2) section Fimbriata, which was synonymised into section Attenuata in the latest system but is morphologically distinct from the other species, is redefined as a monotypic section. In conclusion, we recognize 15 sections of Arisaema and species-level classifications are discussed in a phylogenetic context.

  • morphological patterns in the plumular leaf of japanese Arisaema araceae
    APG : Acta phytotaxonomica et geobotanica, 2014
    Co-Authors: Tomiki Kobayashi, Junichi Ohno, Jin Murata
    Abstract:

    The morphology of the plumular leaf was investigated for the 36 taxa (31 species and 5 subpsecies) of Arisaema (Araceae) in Japan. Of the total, 22 taxa (18 species and 4 subspecies) were newly observ

  • Arisaema maekawae a new species of the Arisaema serratum group araceae from japan
    APG : Acta phytotaxonomica et geobotanica, 2008
    Co-Authors: Jin Murata, Satoshi Kakishima
    Abstract:

    Arisaema maekawae J. Murata & S. Kakishima, a new species of the Arisaema serratum group (Araceae), is described from Japan. This species is most similar to A. angustatum but distinct in the ventrally

  • Arisaema inaense and a nagiense two diploid species of the a ovale group araceae
    APG : Acta phytotaxonomica et geobotanica, 2008
    Co-Authors: Tomiki Kobayashi, Kuniaki Watanabe, Kazuyuki Sasamura, Jin Murata
    Abstract:

    Based on morphology and chromosome numbers, Arisaema inaense (Seriz.) K. Sasamura et J. Murata is newly recognized at the rank of species and a new species A. nagiense T. Kobayashi, K. Sasamura et J.

  • two new putative natural hybrids in japanese Arisaema araceae
    APG : Acta phytotaxonomica et geobotanica, 2005
    Co-Authors: Tomiki Kobayashi, Jin Murata, Kuniaki Watanabe
    Abstract:

    Two new putative natural hybrids, Arisaema limbatum Nakai x A. ringens (Thunb.) Schott and A. ovale Nakai var. sadoense (Nakai) J. Murata x A. monophyllum Nakai have been reported. The former hybrid w

Kexuan Tang - One of the best experts on this subject based on the ideXlab platform.

  • expression and purification of Arisaema heterophyllum agglutinin in escherichia coli
    Journal of Plant Physiology, 2006
    Co-Authors: Xiuyun Zhao, Kexuan Tang, Xiaofen Sun, Juan Lin, Zhonghai Chen, Weiwen Kong
    Abstract:

    Summary Recombinant Arisaema heterophyllum agglutinin (AHA) was expressed in Escherichia coli as N-terminal His-tagged fusions. After induction with isopropylthio- β - d -galactoside, the recombinant AHA was purified by metal-affinity chromatography. The purified AHA protein was incorporated into artificial diet at 0.1% (w/v) concentration in insect bioassay trial and the result showed that artificial diet containing AHA could significantly inhibit the growth of the third-instar nymphs of peach potato aphid ( Myzus persicae ). This study suggested that AHA could be an effective candidate for the control of peach potato aphid, one of the most serious sap-sucking insect pests causing significant yield loss of crops.

  • cloning and characterization of an agglutinin gene from Arisaema lobatum
    Bioscience Reports, 2005
    Co-Authors: Xiaofen Sun, Juan Lin, Xuanwei Zhou, Yongzhen Pang, Han Gao, Jiong Fei, Guoan Shen, Jin Wang, Kexuan Tang
    Abstract:

    A novel agglutinin gene was cloned from Arisaema lobatum using SMART RACE-PCR technology. The full-length cDNA of Arisaema lobatum agglutinin (ala) was 1078 bp and contained a 774 bp open reading frame encoding a lectin precursor (proproprotein) of 258 amino acid residues with a 23 aa signal peptide. ALA contained three mannose-binding sites (QXDXNXVXY) with two-conserved domains of 45% identity, ALA-DOM1 and ALA-DOM2. The three-dimensional structure of ALA was very similar to that of GNA (Galanthus nivalis agglutinin). ALA shared varying identities, ranging from 40% to 85%, with mannose-binding lectins from other species of plant families, such as Araceae, Alliaceae, Iridaceae, Lillaceae, Amaryllidaceae and Bromeliaceae. Genomic sequence of ala was also cloned using genomic walker technology, and it was found to contain three putative TATA boxes and eight possible CAAT boxes in the 5′-flanking region. No intron was found within the region of genomic sequence. Southern blot analysis indicated that the ala belonged to a multi-copy gene family. Expression pattern analysis revealed that the ala preferentially expressed in the tissues with the higher expression being found in spadix, bud, leaf, spathe and tuber. The cloning of the ala gene not only provides a basis for further investigation of its structure, expression and regulation mechanism, but also enables us to test its potential role in controlling pests and fungal diseases by transferring the gene into plants in the future.

  • Transgenic tobacco expressing an Arisaema heterophyllum agglutinin gene displays enhanced resistance to aphids
    Canadian Journal of Plant Science, 2004
    Co-Authors: Xiuyun Zhao Jianhong Yao, Xiaofen Sun Fei Chen, Kexuan Tang
    Abstract:

    Tobacco leaf discs were transformed with a plasmid, pBIAHA, containing the selectable marker neomycin phosphotransferase gene (nptII) and an Arisaema heterophyllum agglutinin gene (aha) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blot analyses confirmed that multiple copies of the aha gene had integrated into the plant genome. Northern blot analysis revealed that the aha gene was expressed at various levels in the transgenic plants. Insect bioassay test showed that transgenic plants expressing multiple copies of the aha gene reduced the rate of population increase of the peach potato aphid (Myzus persicae Sulzer). This is the first report that transgenic tobacco plants expressing the aha gene display enhanced resistance to aphids. Key words: Insect bioassay, Arisaema heterophyllum agglutinin, transformation, transgenic tobacco, peach potato aphid (Myzus persicae Sulzer)

  • molecular cloning of a novel mannose binding lectin gene from Arisaema heterophyllum
    Plant Science, 2003
    Co-Authors: Xiuyun Zhao, Jianhong Yao, Zhihua Liao, Hongyu Zhang, Fei Xavier Chen, Lei Wang, Xiaofen Sun, Kexuan Tang
    Abstract:

    A new lectin gene was cloned from Arisaema heterophyllum. The full-length cDNA of Arisaema heterophyllum agglutinin (AHA) was 1169 bp and contained a 777 bp open reading frame encoding a 258 amino acid protein. Homology analysis showed that AHA had high homology with many other mannose-binding lectins. Secondary and three-dimensional analyses showed that AHA had many common characters of mannose-binding lectin superfamily. Southern blot analysis of the genomic DNA revealed that aha belonged to a multigene family. Northern blot analysis demonstrated that aha constitutively expressed in various plant tissues including inflorescence, leaf and tuber, and highly expressed in the tuber.

E. Rozema - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of glucocerebrosides and the active metabolite 4,8-sphingadienine from Arisaema amurense and Pinellia ternata by NMR and CD spectroscopy and ESI-MS/CID-MS.
    Journal of Agricultural and Food Chemistry, 2012
    Co-Authors: E. Rozema, Ruxandra Popescu, Harald Sonderegger, Christian W. Huck, Johannes Winkler, Georg Krupitza, Ernst Urban, Brigitte Kopp
    Abstract:

    Sphingolipid metabolites regulate cellular processes such as cell proliferation, differentiation, and apoptosis. In this study, glucocerebrosides (GluCer) from rhizomes of Arisaema amurense and Pinellia ternata were fully characterized using 1- and 2-dimensional nuclear magnetic spin resonance (NMR) and circular dichroism (CD) spectroscopy and tandem collision-induced dissociation mass spectrometry (ESI-MS/CID-MS). Three new acylated and seven known GluCer were elucidated with 4,8-sphingadienine (4,8-SD, d18:2) as backbone. 4,8-SD is a metabolite after enzymatical hydrolysis of GluCer in the gut lumen. In this study, 4,8-SD was hydrolyzed from GluCer and chromatographically purified on silica gel. In contrast to the GluCer, 4,8-SD showed cytotoxic effects in the WST-1 assay. GluCer with 4,8-SD as sphingoid backbone are present in plants consumed as food, such as spinach, soy, and eggplant.

  • Selected Extracts of Chinese Herbal Medicines: Their Effect on NF-κB, PPARα and PPARγ and the Respective Bioactive Compounds
    Hindawi Limited, 2012
    Co-Authors: E. Rozema, A. G. Atanasov, N. Fakhrudin, J. Singhuber, U. Namduang, E. H. Heiss, G. Reznicek, C. W. Huck, G. K. Bonn, V. M. Dirsch
    Abstract:

    Chinese herbal medicinal (CHM) extracts from fourteen plants were investigated in cell-based in vitro assays for their effect on nuclear factor κB (NF-κB), a key regulator of inflammation, as well as on peroxisome proliferator-activated receptors (PPARs) being key regulators of genes involved in lipid and glucose metabolism. 43% of the investigated CHMs showed NF-κB inhibitory and 50% PPARα and PPARγ activating effects. Apolar extracts from cortex and flos of Albizia julibrissin Durazz. and processed rhizomes of Arisaema sp. and Pinellia ternata (Thunb.) Breit. that effectively inhibited TNF-α-induced NF-κB activation and dose-dependently activated PPARα and PPARγ were further investigated. Bioassay-guided fractionation and analysis by GC-MS led to the identification of fatty acids as PPAR agonists, including linoleic and palmitic acid

  • Characterization of Glucocerebrosides and the Active Metabolite 4,8-Sphingadienine from Arisaema amurense and Pinellia ternata by NMR and CD Spectroscopy and ESI-MS/CID-MS
    2012
    Co-Authors: E. Rozema, Ruxandra Popescu, Harald Sonderegger, Johannes Winkler, Georg Krupitza, Ernst Urban, Christian W. Huck, Brigitte Kopp
    Abstract:

    Sphingolipid metabolites regulate cellular processes such as cell proliferation, differentiation, and apoptosis. In this study, glucocerebrosides (GluCer) from rhizomes of Arisaema amurense and Pinellia ternata were fully characterized using 1- and 2-dimensional nuclear magnetic spin resonance (NMR) and circular dichroism (CD) spectroscopy and tandem collision-induced dissociation mass spectrometry (ESI-MS/CID-MS). Three new acylated and seven known GluCer were elucidated with 4,8-sphingadienine (4,8-SD, d18:2) as backbone. 4,8-SD is a metabolite after enzymatical hydrolysis of GluCer in the gut lumen. In this study, 4,8-SD was hydrolyzed from GluCer and chromatographically purified on silica gel. In contrast to the GluCer, 4,8-SD showed cytotoxic effects in the WST-1 assay. GluCer with 4,8-SD as sphingoid backbone are present in plants consumed as food, such as spinach, soy, and eggplant

Lesley Lovettdoust - One of the best experts on this subject based on the ideXlab platform.

  • seed germination patterns in green dragon Arisaema dracontium araceae
    American Journal of Botany, 1999
    Co-Authors: J Yang, Jon Lovettdoust, Lesley Lovettdoust
    Abstract:

    Arisaema dracontium (green dragon) is a perennial herb that is widely distributed in eastern North America. However, in Canada, at the northern edge of its distribution, the species is designated as "vulnerable" with respect to conservation status. In natural populations, seedlings are uncommon; the present study was undertaken in order to characterize seed and seedling properties in green dragon. Seeds were sampled from five sites, ranging from Ontario at the northern limit of the distribution range, to Louisiana in the south. Seed germinability ranged from 25 to 55%, depending upon source. Experiments indicated that neither the hard seed coat nor a water-soluble exudate from the seed was responsible for inducing or maintaining dormancy. Patterns of seed germination appear to reflect general climatic conditions at the sites where seeds had originated. Cold stratification at 3°C produced significantly greater relative germinability in all seed collections except the most southerly one, from Baton Rouge. These seeds also had a slower overall speed of germination. In contrast, germination of seeds from the most northerly site was promoted by cold stratification and occurred over a relatively brief period. Germination in alternating light and dark conditions decreased the speed of germination compared to germination in the dark, however exposure to light changed the phenology of germination by promoting development of adventitious roots and primary leaves in these seedlings.