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A P Ryskov - One of the best experts on this subject based on the ideXlab platform.
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multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard darevskia Armeniaca
BMC Genomics, 2018Co-Authors: A E Girnyk, I A Martirosyan, F D Danielyan, A A Vergun, S K Semyenova, Andrei S Guliaev, Marine Arakelyan, Robert W Murphy, A P RyskovAbstract:The parthenogenetic Caucasian rock lizard Darevskia Armeniaca, like most other parthenogenetic vertebrate species, originated through interspecific hybridization between the closely related sexual Darevskia mixta and Darevskia valentini. Darevskia Armeniaca was shown to consist of one widespread allozyme clone and a few rare ones, but notwithstanding the origin of clonal diversity remains unclear. We conduct genomic analysis of D. Armeniaca and its parental sexual species using microsatellite and SNP markers to identify the origin of parthenogenetic clonal lineages. Four microsatellite-containing loci were genotyped for 111 specimens of D. Armeniaca, 17 D. valentini, and four D. mixta. For these species, a total of 47 alleles were isolated and sequenced. Analysis of the data revealed 13 genotypes or presumptive clones in parthenogenetic D. Armeniaca, including one widespread clone, two apparently geographically restricted clones, and ten rare clones. Comparisons of genotype-specific markers in D. Armeniaca with those of its parental species revealed three founder-events including a common and two rare clones. All other clones appeared to have originated via post-formation microsatellite mutations in the course of evolutionary history of D. Armeniaca. Our new approach to microsatellite genotyping reveals allele-specific microsatellite and SNP markers for each locus studied. Interspecies comparison of these markers identifies alleles inherited by parthenospecies from parental species, and provides new information on origin and evolution of clonal diversity in D. Armeniaca. SNP analyses reveal at least three interspecific origins of D. Armeniaca, and microsatellite mutations in these initial clones give rise to new clones. Thus, we first establish multiple origins of D. Armeniaca. Our study identifies the most effective molecular markers for elucidating the origins of clonal diversity in other unisexual species that arose via interspecific hybridization.
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Multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard Darevskia Armeniaca
BMC, 2018Co-Authors: A E Girnyk, I A Martirosyan, F D Danielyan, A A Vergun, S K Semyenova, Andrei S Guliaev, Marine Arakelyan, Robert W Murphy, A P RyskovAbstract:Abstract Background The parthenogenetic Caucasian rock lizard Darevskia Armeniaca, like most other parthenogenetic vertebrate species, originated through interspecific hybridization between the closely related sexual Darevskia mixta and Darevskia valentini. Darevskia Armeniaca was shown to consist of one widespread allozyme clone and a few rare ones, but notwithstanding the origin of clonal diversity remains unclear. We conduct genomic analysis of D. Armeniaca and its parental sexual species using microsatellite and SNP markers to identify the origin of parthenogenetic clonal lineages. Results Four microsatellite-containing loci were genotyped for 111 specimens of D. Armeniaca, 17 D. valentini, and four D. mixta. For these species, a total of 47 alleles were isolated and sequenced. Analysis of the data revealed 13 genotypes or presumptive clones in parthenogenetic D. Armeniaca, including one widespread clone, two apparently geographically restricted clones, and ten rare clones. Comparisons of genotype-specific markers in D. Armeniaca with those of its parental species revealed three founder-events including a common and two rare clones. All other clones appeared to have originated via post-formation microsatellite mutations in the course of evolutionary history of D. Armeniaca. Conclusion Our new approach to microsatellite genotyping reveals allele-specific microsatellite and SNP markers for each locus studied. Interspecies comparison of these markers identifies alleles inherited by parthenospecies from parental species, and provides new information on origin and evolution of clonal diversity in D. Armeniaca. SNP analyses reveal at least three interspecific origins of D. Armeniaca, and microsatellite mutations in these initial clones give rise to new clones. Thus, we first establish multiple origins of D. Armeniaca. Our study identifies the most effective molecular markers for elucidating the origins of clonal diversity in other unisexual species that arose via interspecific hybridization
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variation of mini and microsatellite dna repeats in parthenogenetic lizard darevskia Armeniaca as revealed by dna fingerprinting analysis
Russian Journal of Genetics, 2003Co-Authors: I A Martirosyan, N G Kan, V G Petrosyan, D N Malysheva, A A Trofimova, F D Danielyan, I S Darevskii, L I Korochkin, A P Ryskov, O N TokarskayaAbstract:Population and family samples of two morphological forms (mutant and normal with respect to dorsal color) of parthenogenetic lizard species Darevskia Armeniaca were examined by means of DNA fingerprinting using M13 mini- and (GACA) n and (TCC) n microsatellite DNA markers. The morphological forms examined were characterized by clonally inherited, species-specific patterns of the DNA markers, which were different from the species-specific DNA fingerprints of the other parthenogenetic species of the genus Darevskia (D. dahli, D. unisexualis, and D. rostombekovi). The mean index of similarity (S) obtained for a sample of 36 individuals from three isolated populations using three types of DNA markers was 0.966. This was similar to those observed in D. dahli (0.962) (P > 0.05), but higher than that in D. unisexualis (0.950) (P < 0.05) and D. rostombekovi(0.875) (P < 0.01). Inheritance of M13 minisatellite and (TCC) n microsatellite DNA markers in the F1 offspring of parthenogenetic lizards was examined. It was shown that variability and clonal diversity of the fingerprint phenotypes observed in the populations and families of D. Armeniaca could be at least partly explained by RFLP mutations in microsatellite repeats.
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genetic variation in parthenogenetic caucasian rock lizards of the genus lacerta l dahli l Armeniaca l unisexualis analyzed by dna fingerprinting
Molecular Genetics and Genomics, 2001Co-Authors: O N Tokarskaya, Ilya S. Darevsky, I A Martirosyan, N G Kan, V G Petrosyan, F D Danielyan, V V Grechko, A P RyskovAbstract:Multilocus DNA fingerprinting has been used to study the variability of some mini- and microsatellite sequences in parthenogenetic species of Caucasian rock lizards of the genus Lacerta (L. dahli, L. Armeniaca and L. unisexualis). We demonstrate that these clonally reproducing lizards possess species-specific DNA fingerprints with a low degree of intra- and interpopulation variation. Mean indices of similarity obtained using M13 DNA, (GACA)4 and (TCC)50 as probes were 0.962 and 0.966 in L. dahli and L. Armeniaca, respectively. The mean index of similarity obtained using M13 and GATA probes in L. unisexualis was estimated to be 0.95. However, despite the high degree of band-sharing, variable DNA fragments were revealed in all populations with the microsatellite probes. An particularly high level of variability was observed for (TCC)n microsatellites in populations of L. unisexualis. In fact TCC-derived DNA fingerprints were close to being individual-specific, with a mean index of similarity of 0.824. Fingerprint analysis of parthenogenetic families of L. Armeniaca showed that all maternal fragments were inherited together by the progeny, and no differences in fingerprint patterns were observed. On the other hand, while identical DNA fingerprints were obtained from L. unisexualis families with M13 and (GATA)4 probes, use of the (TCC)50 probe revealed remarkable intrafamily variation in this species. It is assumed that the genetic heterogeneity observed in parthenogenetic populations may be explained, at least in part, by the existence of genetically unstable microsatellite loci. Our data serve to illustrate processes of spontaneous mutagenesis and the initial stages of clonal differentiation in natural populations of the lizard species studied.
F D Danielyan - One of the best experts on this subject based on the ideXlab platform.
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New records of Darevskia Armeniaca (Méhely, 1909) and Darevskia valentini (Boettger, 1892) (Squamata, Sauria, Lacertidae) from Armenia and updated geographic distribution maps
'Pensoft Publishers', 2019Co-Authors: Varos G. Petrosyan, F D Danielyan, Fedor A. Osipov, Vladimir V. Bobrov, Natalia N. Dergunova, Marine ArakelyanAbstract:During field survey in 2018, we recorded in Armenia the occurrences of the parthenogenetic lizard Darevskia Armeniaca and its “paternal” bisexual species D. valentini. Based on our new data and records taken from publications and museums, we update the distribution maps of these species. The new records expand the geographical boundaries of sympatric habitats of these species and provide additional data for understanding the mechanisms of reticulate evolution and hybrid speciation
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multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard darevskia Armeniaca
BMC Genomics, 2018Co-Authors: A E Girnyk, I A Martirosyan, F D Danielyan, A A Vergun, S K Semyenova, Andrei S Guliaev, Marine Arakelyan, Robert W Murphy, A P RyskovAbstract:The parthenogenetic Caucasian rock lizard Darevskia Armeniaca, like most other parthenogenetic vertebrate species, originated through interspecific hybridization between the closely related sexual Darevskia mixta and Darevskia valentini. Darevskia Armeniaca was shown to consist of one widespread allozyme clone and a few rare ones, but notwithstanding the origin of clonal diversity remains unclear. We conduct genomic analysis of D. Armeniaca and its parental sexual species using microsatellite and SNP markers to identify the origin of parthenogenetic clonal lineages. Four microsatellite-containing loci were genotyped for 111 specimens of D. Armeniaca, 17 D. valentini, and four D. mixta. For these species, a total of 47 alleles were isolated and sequenced. Analysis of the data revealed 13 genotypes or presumptive clones in parthenogenetic D. Armeniaca, including one widespread clone, two apparently geographically restricted clones, and ten rare clones. Comparisons of genotype-specific markers in D. Armeniaca with those of its parental species revealed three founder-events including a common and two rare clones. All other clones appeared to have originated via post-formation microsatellite mutations in the course of evolutionary history of D. Armeniaca. Our new approach to microsatellite genotyping reveals allele-specific microsatellite and SNP markers for each locus studied. Interspecies comparison of these markers identifies alleles inherited by parthenospecies from parental species, and provides new information on origin and evolution of clonal diversity in D. Armeniaca. SNP analyses reveal at least three interspecific origins of D. Armeniaca, and microsatellite mutations in these initial clones give rise to new clones. Thus, we first establish multiple origins of D. Armeniaca. Our study identifies the most effective molecular markers for elucidating the origins of clonal diversity in other unisexual species that arose via interspecific hybridization.
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Multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard Darevskia Armeniaca
BMC, 2018Co-Authors: A E Girnyk, I A Martirosyan, F D Danielyan, A A Vergun, S K Semyenova, Andrei S Guliaev, Marine Arakelyan, Robert W Murphy, A P RyskovAbstract:Abstract Background The parthenogenetic Caucasian rock lizard Darevskia Armeniaca, like most other parthenogenetic vertebrate species, originated through interspecific hybridization between the closely related sexual Darevskia mixta and Darevskia valentini. Darevskia Armeniaca was shown to consist of one widespread allozyme clone and a few rare ones, but notwithstanding the origin of clonal diversity remains unclear. We conduct genomic analysis of D. Armeniaca and its parental sexual species using microsatellite and SNP markers to identify the origin of parthenogenetic clonal lineages. Results Four microsatellite-containing loci were genotyped for 111 specimens of D. Armeniaca, 17 D. valentini, and four D. mixta. For these species, a total of 47 alleles were isolated and sequenced. Analysis of the data revealed 13 genotypes or presumptive clones in parthenogenetic D. Armeniaca, including one widespread clone, two apparently geographically restricted clones, and ten rare clones. Comparisons of genotype-specific markers in D. Armeniaca with those of its parental species revealed three founder-events including a common and two rare clones. All other clones appeared to have originated via post-formation microsatellite mutations in the course of evolutionary history of D. Armeniaca. Conclusion Our new approach to microsatellite genotyping reveals allele-specific microsatellite and SNP markers for each locus studied. Interspecies comparison of these markers identifies alleles inherited by parthenospecies from parental species, and provides new information on origin and evolution of clonal diversity in D. Armeniaca. SNP analyses reveal at least three interspecific origins of D. Armeniaca, and microsatellite mutations in these initial clones give rise to new clones. Thus, we first establish multiple origins of D. Armeniaca. Our study identifies the most effective molecular markers for elucidating the origins of clonal diversity in other unisexual species that arose via interspecific hybridization
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variation of mini and microsatellite dna repeats in parthenogenetic lizard darevskia Armeniaca as revealed by dna fingerprinting analysis
Russian Journal of Genetics, 2003Co-Authors: I A Martirosyan, N G Kan, V G Petrosyan, D N Malysheva, A A Trofimova, F D Danielyan, I S Darevskii, L I Korochkin, A P Ryskov, O N TokarskayaAbstract:Population and family samples of two morphological forms (mutant and normal with respect to dorsal color) of parthenogenetic lizard species Darevskia Armeniaca were examined by means of DNA fingerprinting using M13 mini- and (GACA) n and (TCC) n microsatellite DNA markers. The morphological forms examined were characterized by clonally inherited, species-specific patterns of the DNA markers, which were different from the species-specific DNA fingerprints of the other parthenogenetic species of the genus Darevskia (D. dahli, D. unisexualis, and D. rostombekovi). The mean index of similarity (S) obtained for a sample of 36 individuals from three isolated populations using three types of DNA markers was 0.966. This was similar to those observed in D. dahli (0.962) (P > 0.05), but higher than that in D. unisexualis (0.950) (P < 0.05) and D. rostombekovi(0.875) (P < 0.01). Inheritance of M13 minisatellite and (TCC) n microsatellite DNA markers in the F1 offspring of parthenogenetic lizards was examined. It was shown that variability and clonal diversity of the fingerprint phenotypes observed in the populations and families of D. Armeniaca could be at least partly explained by RFLP mutations in microsatellite repeats.
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genetic variation in parthenogenetic caucasian rock lizards of the genus lacerta l dahli l Armeniaca l unisexualis analyzed by dna fingerprinting
Molecular Genetics and Genomics, 2001Co-Authors: O N Tokarskaya, Ilya S. Darevsky, I A Martirosyan, N G Kan, V G Petrosyan, F D Danielyan, V V Grechko, A P RyskovAbstract:Multilocus DNA fingerprinting has been used to study the variability of some mini- and microsatellite sequences in parthenogenetic species of Caucasian rock lizards of the genus Lacerta (L. dahli, L. Armeniaca and L. unisexualis). We demonstrate that these clonally reproducing lizards possess species-specific DNA fingerprints with a low degree of intra- and interpopulation variation. Mean indices of similarity obtained using M13 DNA, (GACA)4 and (TCC)50 as probes were 0.962 and 0.966 in L. dahli and L. Armeniaca, respectively. The mean index of similarity obtained using M13 and GATA probes in L. unisexualis was estimated to be 0.95. However, despite the high degree of band-sharing, variable DNA fragments were revealed in all populations with the microsatellite probes. An particularly high level of variability was observed for (TCC)n microsatellites in populations of L. unisexualis. In fact TCC-derived DNA fingerprints were close to being individual-specific, with a mean index of similarity of 0.824. Fingerprint analysis of parthenogenetic families of L. Armeniaca showed that all maternal fragments were inherited together by the progeny, and no differences in fingerprint patterns were observed. On the other hand, while identical DNA fingerprints were obtained from L. unisexualis families with M13 and (GATA)4 probes, use of the (TCC)50 probe revealed remarkable intrafamily variation in this species. It is assumed that the genetic heterogeneity observed in parthenogenetic populations may be explained, at least in part, by the existence of genetically unstable microsatellite loci. Our data serve to illustrate processes of spontaneous mutagenesis and the initial stages of clonal differentiation in natural populations of the lizard species studied.
I A Martirosyan - One of the best experts on this subject based on the ideXlab platform.
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multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard darevskia Armeniaca
BMC Genomics, 2018Co-Authors: A E Girnyk, I A Martirosyan, F D Danielyan, A A Vergun, S K Semyenova, Andrei S Guliaev, Marine Arakelyan, Robert W Murphy, A P RyskovAbstract:The parthenogenetic Caucasian rock lizard Darevskia Armeniaca, like most other parthenogenetic vertebrate species, originated through interspecific hybridization between the closely related sexual Darevskia mixta and Darevskia valentini. Darevskia Armeniaca was shown to consist of one widespread allozyme clone and a few rare ones, but notwithstanding the origin of clonal diversity remains unclear. We conduct genomic analysis of D. Armeniaca and its parental sexual species using microsatellite and SNP markers to identify the origin of parthenogenetic clonal lineages. Four microsatellite-containing loci were genotyped for 111 specimens of D. Armeniaca, 17 D. valentini, and four D. mixta. For these species, a total of 47 alleles were isolated and sequenced. Analysis of the data revealed 13 genotypes or presumptive clones in parthenogenetic D. Armeniaca, including one widespread clone, two apparently geographically restricted clones, and ten rare clones. Comparisons of genotype-specific markers in D. Armeniaca with those of its parental species revealed three founder-events including a common and two rare clones. All other clones appeared to have originated via post-formation microsatellite mutations in the course of evolutionary history of D. Armeniaca. Our new approach to microsatellite genotyping reveals allele-specific microsatellite and SNP markers for each locus studied. Interspecies comparison of these markers identifies alleles inherited by parthenospecies from parental species, and provides new information on origin and evolution of clonal diversity in D. Armeniaca. SNP analyses reveal at least three interspecific origins of D. Armeniaca, and microsatellite mutations in these initial clones give rise to new clones. Thus, we first establish multiple origins of D. Armeniaca. Our study identifies the most effective molecular markers for elucidating the origins of clonal diversity in other unisexual species that arose via interspecific hybridization.
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Multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard Darevskia Armeniaca
BMC, 2018Co-Authors: A E Girnyk, I A Martirosyan, F D Danielyan, A A Vergun, S K Semyenova, Andrei S Guliaev, Marine Arakelyan, Robert W Murphy, A P RyskovAbstract:Abstract Background The parthenogenetic Caucasian rock lizard Darevskia Armeniaca, like most other parthenogenetic vertebrate species, originated through interspecific hybridization between the closely related sexual Darevskia mixta and Darevskia valentini. Darevskia Armeniaca was shown to consist of one widespread allozyme clone and a few rare ones, but notwithstanding the origin of clonal diversity remains unclear. We conduct genomic analysis of D. Armeniaca and its parental sexual species using microsatellite and SNP markers to identify the origin of parthenogenetic clonal lineages. Results Four microsatellite-containing loci were genotyped for 111 specimens of D. Armeniaca, 17 D. valentini, and four D. mixta. For these species, a total of 47 alleles were isolated and sequenced. Analysis of the data revealed 13 genotypes or presumptive clones in parthenogenetic D. Armeniaca, including one widespread clone, two apparently geographically restricted clones, and ten rare clones. Comparisons of genotype-specific markers in D. Armeniaca with those of its parental species revealed three founder-events including a common and two rare clones. All other clones appeared to have originated via post-formation microsatellite mutations in the course of evolutionary history of D. Armeniaca. Conclusion Our new approach to microsatellite genotyping reveals allele-specific microsatellite and SNP markers for each locus studied. Interspecies comparison of these markers identifies alleles inherited by parthenospecies from parental species, and provides new information on origin and evolution of clonal diversity in D. Armeniaca. SNP analyses reveal at least three interspecific origins of D. Armeniaca, and microsatellite mutations in these initial clones give rise to new clones. Thus, we first establish multiple origins of D. Armeniaca. Our study identifies the most effective molecular markers for elucidating the origins of clonal diversity in other unisexual species that arose via interspecific hybridization
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variation of mini and microsatellite dna repeats in parthenogenetic lizard darevskia Armeniaca as revealed by dna fingerprinting analysis
Russian Journal of Genetics, 2003Co-Authors: I A Martirosyan, N G Kan, V G Petrosyan, D N Malysheva, A A Trofimova, F D Danielyan, I S Darevskii, L I Korochkin, A P Ryskov, O N TokarskayaAbstract:Population and family samples of two morphological forms (mutant and normal with respect to dorsal color) of parthenogenetic lizard species Darevskia Armeniaca were examined by means of DNA fingerprinting using M13 mini- and (GACA) n and (TCC) n microsatellite DNA markers. The morphological forms examined were characterized by clonally inherited, species-specific patterns of the DNA markers, which were different from the species-specific DNA fingerprints of the other parthenogenetic species of the genus Darevskia (D. dahli, D. unisexualis, and D. rostombekovi). The mean index of similarity (S) obtained for a sample of 36 individuals from three isolated populations using three types of DNA markers was 0.966. This was similar to those observed in D. dahli (0.962) (P > 0.05), but higher than that in D. unisexualis (0.950) (P < 0.05) and D. rostombekovi(0.875) (P < 0.01). Inheritance of M13 minisatellite and (TCC) n microsatellite DNA markers in the F1 offspring of parthenogenetic lizards was examined. It was shown that variability and clonal diversity of the fingerprint phenotypes observed in the populations and families of D. Armeniaca could be at least partly explained by RFLP mutations in microsatellite repeats.
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genetic variation in parthenogenetic caucasian rock lizards of the genus lacerta l dahli l Armeniaca l unisexualis analyzed by dna fingerprinting
Molecular Genetics and Genomics, 2001Co-Authors: O N Tokarskaya, Ilya S. Darevsky, I A Martirosyan, N G Kan, V G Petrosyan, F D Danielyan, V V Grechko, A P RyskovAbstract:Multilocus DNA fingerprinting has been used to study the variability of some mini- and microsatellite sequences in parthenogenetic species of Caucasian rock lizards of the genus Lacerta (L. dahli, L. Armeniaca and L. unisexualis). We demonstrate that these clonally reproducing lizards possess species-specific DNA fingerprints with a low degree of intra- and interpopulation variation. Mean indices of similarity obtained using M13 DNA, (GACA)4 and (TCC)50 as probes were 0.962 and 0.966 in L. dahli and L. Armeniaca, respectively. The mean index of similarity obtained using M13 and GATA probes in L. unisexualis was estimated to be 0.95. However, despite the high degree of band-sharing, variable DNA fragments were revealed in all populations with the microsatellite probes. An particularly high level of variability was observed for (TCC)n microsatellites in populations of L. unisexualis. In fact TCC-derived DNA fingerprints were close to being individual-specific, with a mean index of similarity of 0.824. Fingerprint analysis of parthenogenetic families of L. Armeniaca showed that all maternal fragments were inherited together by the progeny, and no differences in fingerprint patterns were observed. On the other hand, while identical DNA fingerprints were obtained from L. unisexualis families with M13 and (GATA)4 probes, use of the (TCC)50 probe revealed remarkable intrafamily variation in this species. It is assumed that the genetic heterogeneity observed in parthenogenetic populations may be explained, at least in part, by the existence of genetically unstable microsatellite loci. Our data serve to illustrate processes of spontaneous mutagenesis and the initial stages of clonal differentiation in natural populations of the lizard species studied.
A A Vergun - One of the best experts on this subject based on the ideXlab platform.
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multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard darevskia Armeniaca
BMC Genomics, 2018Co-Authors: A E Girnyk, I A Martirosyan, F D Danielyan, A A Vergun, S K Semyenova, Andrei S Guliaev, Marine Arakelyan, Robert W Murphy, A P RyskovAbstract:The parthenogenetic Caucasian rock lizard Darevskia Armeniaca, like most other parthenogenetic vertebrate species, originated through interspecific hybridization between the closely related sexual Darevskia mixta and Darevskia valentini. Darevskia Armeniaca was shown to consist of one widespread allozyme clone and a few rare ones, but notwithstanding the origin of clonal diversity remains unclear. We conduct genomic analysis of D. Armeniaca and its parental sexual species using microsatellite and SNP markers to identify the origin of parthenogenetic clonal lineages. Four microsatellite-containing loci were genotyped for 111 specimens of D. Armeniaca, 17 D. valentini, and four D. mixta. For these species, a total of 47 alleles were isolated and sequenced. Analysis of the data revealed 13 genotypes or presumptive clones in parthenogenetic D. Armeniaca, including one widespread clone, two apparently geographically restricted clones, and ten rare clones. Comparisons of genotype-specific markers in D. Armeniaca with those of its parental species revealed three founder-events including a common and two rare clones. All other clones appeared to have originated via post-formation microsatellite mutations in the course of evolutionary history of D. Armeniaca. Our new approach to microsatellite genotyping reveals allele-specific microsatellite and SNP markers for each locus studied. Interspecies comparison of these markers identifies alleles inherited by parthenospecies from parental species, and provides new information on origin and evolution of clonal diversity in D. Armeniaca. SNP analyses reveal at least three interspecific origins of D. Armeniaca, and microsatellite mutations in these initial clones give rise to new clones. Thus, we first establish multiple origins of D. Armeniaca. Our study identifies the most effective molecular markers for elucidating the origins of clonal diversity in other unisexual species that arose via interspecific hybridization.
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Multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard Darevskia Armeniaca
BMC, 2018Co-Authors: A E Girnyk, I A Martirosyan, F D Danielyan, A A Vergun, S K Semyenova, Andrei S Guliaev, Marine Arakelyan, Robert W Murphy, A P RyskovAbstract:Abstract Background The parthenogenetic Caucasian rock lizard Darevskia Armeniaca, like most other parthenogenetic vertebrate species, originated through interspecific hybridization between the closely related sexual Darevskia mixta and Darevskia valentini. Darevskia Armeniaca was shown to consist of one widespread allozyme clone and a few rare ones, but notwithstanding the origin of clonal diversity remains unclear. We conduct genomic analysis of D. Armeniaca and its parental sexual species using microsatellite and SNP markers to identify the origin of parthenogenetic clonal lineages. Results Four microsatellite-containing loci were genotyped for 111 specimens of D. Armeniaca, 17 D. valentini, and four D. mixta. For these species, a total of 47 alleles were isolated and sequenced. Analysis of the data revealed 13 genotypes or presumptive clones in parthenogenetic D. Armeniaca, including one widespread clone, two apparently geographically restricted clones, and ten rare clones. Comparisons of genotype-specific markers in D. Armeniaca with those of its parental species revealed three founder-events including a common and two rare clones. All other clones appeared to have originated via post-formation microsatellite mutations in the course of evolutionary history of D. Armeniaca. Conclusion Our new approach to microsatellite genotyping reveals allele-specific microsatellite and SNP markers for each locus studied. Interspecies comparison of these markers identifies alleles inherited by parthenospecies from parental species, and provides new information on origin and evolution of clonal diversity in D. Armeniaca. SNP analyses reveal at least three interspecific origins of D. Armeniaca, and microsatellite mutations in these initial clones give rise to new clones. Thus, we first establish multiple origins of D. Armeniaca. Our study identifies the most effective molecular markers for elucidating the origins of clonal diversity in other unisexual species that arose via interspecific hybridization
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Additional file 4: of Multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard Darevskia Armeniaca
2018Co-Authors: A E Girnyk, A A Vergun, S K Semyenova, Andrei S Guliaev, Marine Arakelyan, Felix Danielyan, Irena Martirosyan, Robert Murphy, Alexey RyskovAbstract:Figure S1. Schematic representation of the TCS network that reflects distribution of genotypes 1â9 in D. Armeniaca. Concatenated sequences of D. Armeniaca genotypes were analyzed using TCS software v.1.21. Genotypes 10â13 are plotted separately. Population distribution of the genotypes is shown by different colors. Numbers indicate the number of individuals in populations. The black circles show, unsampled, but computer-predicted genotypes. (TIF 802 kb
Wanpeng Xi - One of the best experts on this subject based on the ideXlab platform.
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transcriptional regulatory networks controlling taste and aroma quality of apricot prunus Armeniaca l fruit during ripening
BMC Genomics, 2019Co-Authors: Qiuyun Zhang, Zehui Qu, Wenhui Li, Ming Zeng, Chao Feng, Wanpeng XiAbstract:Background Taste and aroma, which are important organoleptic qualities of apricot (Prunus Armeniaca L.) fruit, undergo rapid and substantial changes during ripening. However, the associated molecular mechanisms remain unclear. The goal of this study was to identify candidate genes for flavor compound metabolism and to construct a regulatory transcriptional network.