The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Eglantine Heude - One of the best experts on this subject based on the ideXlab platform.
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Dorsal midline defects in perinatal Dlx5/6-/- mice.
2019Co-Authors: Nicolas Narboux-neme, Marc Ekker, Giovanni Levi, Eglantine HeudeAbstract:(A-D) Macroscopic dorsal view (A-B) and skeletal preparation (C-D) of the posterior axis of control Dlx5/6+/- and Dlx5/6-/- mutant mice at E18.5 (n = 6 for each condition). (E-F) Immunostaining on coronal cryosections for Tnnt3 in dorsal musculature of control Dlx5/6+/- and Dlx5/6-/- E18.5 foetuses (n = 3 for each condition). Dlx5/6-/- mutants display a dorsal split already evident at macroscopic inspection (B, red Arrowheads). This phenotype is associated with defects of thoracic/lumbar vertebrae (D, red Arrowhead) and of epaxial muscle formation at the dorsal midline (F, red Arrowheads). Abbreviations: em, epaxial muscles. Scale bars in B and D 2000 μm and in E 200 μm.
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Early phenotype of Dlx5/6-inactivated zebrafish and mouse.
2019Co-Authors: Nicolas Narboux-neme, Marc Ekker, Giovanni Levi, Eglantine HeudeAbstract:(A-B) Phenotype of wild type (WT) and dlx5a/6a morphant zebrafish at 48 hpf (n>750 for each condition). (C-D) Phenotype of WT and Dlx5/6-/- mutant mice at E11.5 (n = 6 for each condition). Inactivation of Dlx5/6 in zebrafish and mouse leads to early defect of posterior axis development characterized by curly-shaped tail phenotype in both models (B, D, white Arrowheads). In Dlx5/6-/- embryos, the caudal phenotype is associated with defect of brain formation (D, blue Arrowhead). Scale bar in B for A-B 100 μm, for C-D 1000 μm.
Mark R Prausnitz - One of the best experts on this subject based on the ideXlab platform.
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separable Arrowhead microneedles
Journal of Controlled Release, 2011Co-Authors: Mark R PrausnitzAbstract:Hypodermic needles cause pain and bleeding, produce biohazardous sharp waste and require trained personnel. To address these issues, we introduce separable Arrowhead microneedles that rapidly and painlessly deliver drugs and vaccines to the skin. These needles are featured by micron-size sharp tips mounted on blunt shafts. Upon insertion in the skin, the sharp-tipped polymer Arrowheads encapsulating drug separate from their metal shafts and remain embedded in the skin for subsequent dissolution and drug release. The blunt metal shafts can then be discarded. Due to rapid separation of the Arrowhead tips from the shafts within seconds, administration using Arrowhead microneedles can be carried out rapidly, while drug release kinetics can be independently controlled based on separable Arrowhead formulation. Thus, drug and vaccine delivery using Arrowhead microneedles are designed to offer a quick, convenient, safe and potentially self-administered method of drug delivery as an alternative to hypodermic needles.
Benoit Cousineau - One of the best experts on this subject based on the ideXlab platform.
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Detection of intermediates unique to the reverse splicing pathway: Ll.LtrB reverse-spliced within L. lactis mRNAs, E1-mRNA and mRNA-mRNA chimeras.
2018Co-Authors: Félix Laroche-johnston, Caroline Monat, Samy Coulombe, Benoit CousineauAbstract:RT-PCR assays were performed to detect the 5’ and 3’ junctions of Ll.LtrB-ΔLtrA+LtrA and Ll.LtrB-ΔA-ΔLtrA+LtrA reverse splicing events within the enoA (red boxes)(a, b) and alaS (blue boxes)(e, f) mRNAs. Complete and dashed arrows indicate reverse splicing of Ll.LtrB lariats within strong (S) and weak (W) IBS1/2-like sequences (—|—) respectively. The strong (S)(10/11 nts)(large Arrowhead) and weak (W)(7-9/11 nts)(small Arrowhead) IBS1/2-like sequences invaded by reverse splicing are represented (c, g). The sites flanking the mRNA fragments (c, 167 nts and g, 304 nts) initially detected at intron circle splice junctions (Fig 3A) are indicated by open Arrowheads. The Ll.LtrB insertion sites were identified in conditions where the enoA or the alaS genes were overexpressed (small open and black Arrowheads) or not (large open and small gray Arrowheads) from a P23 constitutive promoter. mRNA chimeras between ltrB-exon 1 (E1) and L. lactis mRNAs (d, E1-enoA)(h, E1-alaS) as well as between L. lactis mRNAs (i, alaS-enoA)(j, enoA-alaS) were also detected by RT-PCR at IBS1/2-like sequences.
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Detection of intermediates unique to the reverse splicing pathway: Ll.LtrB reverse-spliced within an L. lactis mRNA and an E1-mRNA chimera.
2018Co-Authors: Félix Laroche-johnston, Caroline Monat, Samy Coulombe, Benoit CousineauAbstract:RT-PCR assays were performed to detect the 5’ and 3’ junctions of Ll.LtrB-EBS1/Mut-ΔLtrA+LtrA and Ll.LtrB-ΔA-EBS1/Mut-ΔLtrA+LtrA reverse splicing events within the Ribosomal Protein S12/S7 mRNA (a, b). Complete and dashed arrows indicate reverse splicing of Ll.LtrB lariats within strong (S) and weak (W) IBS1/2-like sequences (—|—) respectively. The strong (S)(10/11 nts)(large Arrowhead) and weak (W)(8-9/11 nts)(small Arrowheads) IBS1/2-like sequences invaded by reverse splicing are represented (c). The sites flanking the mRNA fragment (c, 161 nts) initially detected at intron circle splice junctions (Fig 3B) are indicated by open Arrowheads. mRNA chimeras between ltrB-exon 1 (E1) and L. lactis mRNAs (d, E1-S12/S7) were also detected by RT-PCR at IBS1/2-like sequences.
Nicolas Narboux-neme - One of the best experts on this subject based on the ideXlab platform.
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Dorsal midline defects in perinatal Dlx5/6-/- mice.
2019Co-Authors: Nicolas Narboux-neme, Marc Ekker, Giovanni Levi, Eglantine HeudeAbstract:(A-D) Macroscopic dorsal view (A-B) and skeletal preparation (C-D) of the posterior axis of control Dlx5/6+/- and Dlx5/6-/- mutant mice at E18.5 (n = 6 for each condition). (E-F) Immunostaining on coronal cryosections for Tnnt3 in dorsal musculature of control Dlx5/6+/- and Dlx5/6-/- E18.5 foetuses (n = 3 for each condition). Dlx5/6-/- mutants display a dorsal split already evident at macroscopic inspection (B, red Arrowheads). This phenotype is associated with defects of thoracic/lumbar vertebrae (D, red Arrowhead) and of epaxial muscle formation at the dorsal midline (F, red Arrowheads). Abbreviations: em, epaxial muscles. Scale bars in B and D 2000 μm and in E 200 μm.
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Early phenotype of Dlx5/6-inactivated zebrafish and mouse.
2019Co-Authors: Nicolas Narboux-neme, Marc Ekker, Giovanni Levi, Eglantine HeudeAbstract:(A-B) Phenotype of wild type (WT) and dlx5a/6a morphant zebrafish at 48 hpf (n>750 for each condition). (C-D) Phenotype of WT and Dlx5/6-/- mutant mice at E11.5 (n = 6 for each condition). Inactivation of Dlx5/6 in zebrafish and mouse leads to early defect of posterior axis development characterized by curly-shaped tail phenotype in both models (B, D, white Arrowheads). In Dlx5/6-/- embryos, the caudal phenotype is associated with defect of brain formation (D, blue Arrowhead). Scale bar in B for A-B 100 μm, for C-D 1000 μm.
Mark F A Vanberkum - One of the best experts on this subject based on the ideXlab platform.
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Increasing Abl activity in a fra mutant reduces commissure formation.
2013Co-Authors: Joy N Dorsten, Bridget E Varughese, Stephanie Karmo, Mark A Seeger, Mark F A VanberkumAbstract:Stage 16 embryos stained with mAb BP102 are depicted with anterior up. Indicated UAS- (U) transgenes are expressed pan-neurally using the 1407-Gal4 (1407-G4) driver line recombined onto a fra3 chromosome. For this reason, phenotypes are compared to heterozygous fra3 rather than a wild-type embryo; in most cases the heterozygote is similar to wild-type. [A] Normal anterior commissures (AC), posterior commissures (PC) and longitudinal connectives (LC) are observed in a fra3 1407-GAL4 heterozygote stained with BP102. [B] Over-expression of BcrAbl in a fra3 heterozygote causes fuzzy commissures in most segments while [C] expression of Ablwt causes only a rare fuzzy commissure (black Arrowheads). [D] Homozygous fra3/fra4 mutants display thinning and missing PC's (black Arrowhead) in some segments but the AC remains unaffected (white Arrowhead). Over-expression of either [E] BcrAbl or [F] Ablwt in a fra3/fra4 background enhances the degree of thinning and missing PC's (black Arrowhead), and many AC's (white Arrowhead) disappear. [K] Quantification of defects in the AC and PC are graphed with percent of commissures missing in a fra3 1407-Gal4 heterozygote (white, highlighted with ‘w’), fra3 1407-Gal4/fra4 homozygote (black) and fra3 1407-Gal4/fra4 homozygote's expressing the indicated Abl transgene (gray). All three Abl transgenes significantly enhance the loss of PCs and both BcrAbl and Ablwt affect AC formation (*P
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Expression of RacV12 and Cdc42V12 reduces commissure formation in a fra mutant.
2013Co-Authors: Joy N Dorsten, Bridget E Varughese, Stephanie Karmo, Mark A Seeger, Mark F A VanberkumAbstract:Stage 16 embryos stained with the mAb BP102 are depicted with anterior up. UAS (U) transgenes are expressed pan-neurally using the 1407-GAL4 (1407-G4) driver line recombined onto the fra3 chromosome, and, as in other figures, phenotypes are compared to heterozygous fra3. Phenotypes in heterozygous fra3 embryos tend to be somewhat milder than that observed in a wild-type embryo (see text). [A] Expression of Cdc42V12 in a fra3 heterozygote background results in fused commissures [Arrowhead] and large gaps in the longitudinal connectives. [B] Expression of RacV12 in a fra3 heterozygote causes a thinning of both PC (black Arrowhead) and AC (white Arrowhead) as well as LC (black arrow). [C] Expression of ctMLCK in a fra3 heterozygote results in fuzzy commissures (black Arrowhead). Expression of both [D] Cdc42V12 and [E] RacV12 in a fra3/fra4 mutant significantly reduces commissure formation (black Arrowheads) and, with expression of RacV12, some axons may orientate towards the periphery (arrow in E), but they are not observed to exit the CNS. [F] Expression of ctMLCK in a fra3/fra4 mutant does not significantly alter the PC (Arrowhead) and LC defects normally observed in a fra mutant.
