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Seyed Hossein Hoseinifar - One of the best experts on this subject based on the ideXlab platform.
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the effects of feeding with synbiotic pediococcus acidilactici and fructooligosaccharide enriched adult Artemia on skin mucus immune responses stress resistance intestinal microbiota and performance of angelfish pterophyllum scalare
Fish & Shellfish Immunology, 2016Co-Authors: Mahmood Azimirad, Saeed Meshkini, Nasrollah Ahmadifard, Seyed Hossein HoseinifarAbstract:Abstract The aim of this study was to evaluate the effects of feeding on synbiotic ( Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish ( Pterophyllum scalare ). Three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 L) assigned to four groups in triplicates. Fish were fed for 7 weeks with dietary treatments, including treatment 1: feeding adult Artemia without enrichment (control group), treatment 2: feeding adult Artemia enriched with lyophilised probiotic P. acidilactici (700 mg L −1 ), 3: feeding adult Artemia enriched with prebiotic fructooligosaccharide (FOS) (100 mg L −1 ), group 4: feeding adult Artemia enriched with synbiotic ( P. acidilactici (700 mg L −1 ) + FOS (100 mg L −1 )). Skin mucus immune responses (lysozyme activity, total Immunoglobulin and protease), stress resistance against environmental stress (acute decrease of temperature and increase salinity), intestinal microbiota as well as growth indices were measured at the end of feeding trial. Artemia enriched with synbiotic significantly improved growth performance compared to other treatments ( P P P Artemia enriched with synbiotic ( P angelfish with synbiotic enriched Artemia was more effective than singular enrichment with probiotics or prebiotics.
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the effects of feeding with synbiotic pediococcus acidilactici and fructooligosaccharide enriched adult Artemia on skin mucus immune responses stress resistance intestinal microbiota and performance of angelfish pterophyllum scalare
Fish & Shellfish Immunology, 2016Co-Authors: Mahmood Azimirad, Saeed Meshkini, Nasrollah Ahmadifard, Seyed Hossein HoseinifarAbstract:The aim of this study was to evaluate the effects of feeding on synbiotic (Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish (Pterophyllum scalare). Three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 L) assigned to four groups in triplicates. Fish were fed for 7 weeks with dietary treatments, including treatment 1: feeding adult Artemia without enrichment (control group), treatment 2: feeding adult Artemia enriched with lyophilised probiotic P. acidilactici (700 mg L(-1)), 3: feeding adult Artemia enriched with prebiotic fructooligosaccharide (FOS) (100 mg L(-1)), group 4: feeding adult Artemia enriched with synbiotic (P. acidilactici (700 mg L(-1)) + FOS (100 mg L(-1))). Skin mucus immune responses (lysozyme activity, total Immunoglobulin and protease), stress resistance against environmental stress (acute decrease of temperature and increase salinity), intestinal microbiota as well as growth indices were measured at the end of feeding trial. Artemia enriched with synbiotic significantly improved growth performance compared to other treatments (P < 0.05). The highest weight gain and specific growth rate (SGR) was observed in synbiotic fed fish (P < 0.05). Compared to the other treatments, the population of lactic acid bacteria was significantly higher in the intestinal microbiota of fish fed synbiotic supplemented diet (P < 0.05). In the environmental stress challenge test, the maximum resistance to abrupt decrease of temperature (17 °C) or elevation of salinity (12 g per liter) was observed in the synbiotic treatment. Also, the total immunoglobulin and lysozyme activity level of skin mucus was significantly elevated in fish fed Artemia enriched with synbiotic (P < 0.05). These results revealed that feeding angelfish with synbiotic enriched Artemia was more effective than singular enrichment with probiotics or prebiotics.
Mahmood Azimirad - One of the best experts on this subject based on the ideXlab platform.
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the effects of feeding with synbiotic pediococcus acidilactici and fructooligosaccharide enriched adult Artemia on skin mucus immune responses stress resistance intestinal microbiota and performance of angelfish pterophyllum scalare
Fish & Shellfish Immunology, 2016Co-Authors: Mahmood Azimirad, Saeed Meshkini, Nasrollah Ahmadifard, Seyed Hossein HoseinifarAbstract:Abstract The aim of this study was to evaluate the effects of feeding on synbiotic ( Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish ( Pterophyllum scalare ). Three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 L) assigned to four groups in triplicates. Fish were fed for 7 weeks with dietary treatments, including treatment 1: feeding adult Artemia without enrichment (control group), treatment 2: feeding adult Artemia enriched with lyophilised probiotic P. acidilactici (700 mg L −1 ), 3: feeding adult Artemia enriched with prebiotic fructooligosaccharide (FOS) (100 mg L −1 ), group 4: feeding adult Artemia enriched with synbiotic ( P. acidilactici (700 mg L −1 ) + FOS (100 mg L −1 )). Skin mucus immune responses (lysozyme activity, total Immunoglobulin and protease), stress resistance against environmental stress (acute decrease of temperature and increase salinity), intestinal microbiota as well as growth indices were measured at the end of feeding trial. Artemia enriched with synbiotic significantly improved growth performance compared to other treatments ( P P P Artemia enriched with synbiotic ( P angelfish with synbiotic enriched Artemia was more effective than singular enrichment with probiotics or prebiotics.
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the effects of feeding with synbiotic pediococcus acidilactici and fructooligosaccharide enriched adult Artemia on skin mucus immune responses stress resistance intestinal microbiota and performance of angelfish pterophyllum scalare
Fish & Shellfish Immunology, 2016Co-Authors: Mahmood Azimirad, Saeed Meshkini, Nasrollah Ahmadifard, Seyed Hossein HoseinifarAbstract:The aim of this study was to evaluate the effects of feeding on synbiotic (Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish (Pterophyllum scalare). Three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 L) assigned to four groups in triplicates. Fish were fed for 7 weeks with dietary treatments, including treatment 1: feeding adult Artemia without enrichment (control group), treatment 2: feeding adult Artemia enriched with lyophilised probiotic P. acidilactici (700 mg L(-1)), 3: feeding adult Artemia enriched with prebiotic fructooligosaccharide (FOS) (100 mg L(-1)), group 4: feeding adult Artemia enriched with synbiotic (P. acidilactici (700 mg L(-1)) + FOS (100 mg L(-1))). Skin mucus immune responses (lysozyme activity, total Immunoglobulin and protease), stress resistance against environmental stress (acute decrease of temperature and increase salinity), intestinal microbiota as well as growth indices were measured at the end of feeding trial. Artemia enriched with synbiotic significantly improved growth performance compared to other treatments (P < 0.05). The highest weight gain and specific growth rate (SGR) was observed in synbiotic fed fish (P < 0.05). Compared to the other treatments, the population of lactic acid bacteria was significantly higher in the intestinal microbiota of fish fed synbiotic supplemented diet (P < 0.05). In the environmental stress challenge test, the maximum resistance to abrupt decrease of temperature (17 °C) or elevation of salinity (12 g per liter) was observed in the synbiotic treatment. Also, the total immunoglobulin and lysozyme activity level of skin mucus was significantly elevated in fish fed Artemia enriched with synbiotic (P < 0.05). These results revealed that feeding angelfish with synbiotic enriched Artemia was more effective than singular enrichment with probiotics or prebiotics.
Nasrollah Ahmadifard - One of the best experts on this subject based on the ideXlab platform.
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the effects of feeding with synbiotic pediococcus acidilactici and fructooligosaccharide enriched adult Artemia on skin mucus immune responses stress resistance intestinal microbiota and performance of angelfish pterophyllum scalare
Fish & Shellfish Immunology, 2016Co-Authors: Mahmood Azimirad, Saeed Meshkini, Nasrollah Ahmadifard, Seyed Hossein HoseinifarAbstract:Abstract The aim of this study was to evaluate the effects of feeding on synbiotic ( Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish ( Pterophyllum scalare ). Three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 L) assigned to four groups in triplicates. Fish were fed for 7 weeks with dietary treatments, including treatment 1: feeding adult Artemia without enrichment (control group), treatment 2: feeding adult Artemia enriched with lyophilised probiotic P. acidilactici (700 mg L −1 ), 3: feeding adult Artemia enriched with prebiotic fructooligosaccharide (FOS) (100 mg L −1 ), group 4: feeding adult Artemia enriched with synbiotic ( P. acidilactici (700 mg L −1 ) + FOS (100 mg L −1 )). Skin mucus immune responses (lysozyme activity, total Immunoglobulin and protease), stress resistance against environmental stress (acute decrease of temperature and increase salinity), intestinal microbiota as well as growth indices were measured at the end of feeding trial. Artemia enriched with synbiotic significantly improved growth performance compared to other treatments ( P P P Artemia enriched with synbiotic ( P angelfish with synbiotic enriched Artemia was more effective than singular enrichment with probiotics or prebiotics.
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the effects of feeding with synbiotic pediococcus acidilactici and fructooligosaccharide enriched adult Artemia on skin mucus immune responses stress resistance intestinal microbiota and performance of angelfish pterophyllum scalare
Fish & Shellfish Immunology, 2016Co-Authors: Mahmood Azimirad, Saeed Meshkini, Nasrollah Ahmadifard, Seyed Hossein HoseinifarAbstract:The aim of this study was to evaluate the effects of feeding on synbiotic (Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish (Pterophyllum scalare). Three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 L) assigned to four groups in triplicates. Fish were fed for 7 weeks with dietary treatments, including treatment 1: feeding adult Artemia without enrichment (control group), treatment 2: feeding adult Artemia enriched with lyophilised probiotic P. acidilactici (700 mg L(-1)), 3: feeding adult Artemia enriched with prebiotic fructooligosaccharide (FOS) (100 mg L(-1)), group 4: feeding adult Artemia enriched with synbiotic (P. acidilactici (700 mg L(-1)) + FOS (100 mg L(-1))). Skin mucus immune responses (lysozyme activity, total Immunoglobulin and protease), stress resistance against environmental stress (acute decrease of temperature and increase salinity), intestinal microbiota as well as growth indices were measured at the end of feeding trial. Artemia enriched with synbiotic significantly improved growth performance compared to other treatments (P < 0.05). The highest weight gain and specific growth rate (SGR) was observed in synbiotic fed fish (P < 0.05). Compared to the other treatments, the population of lactic acid bacteria was significantly higher in the intestinal microbiota of fish fed synbiotic supplemented diet (P < 0.05). In the environmental stress challenge test, the maximum resistance to abrupt decrease of temperature (17 °C) or elevation of salinity (12 g per liter) was observed in the synbiotic treatment. Also, the total immunoglobulin and lysozyme activity level of skin mucus was significantly elevated in fish fed Artemia enriched with synbiotic (P < 0.05). These results revealed that feeding angelfish with synbiotic enriched Artemia was more effective than singular enrichment with probiotics or prebiotics.
Saeed Meshkini - One of the best experts on this subject based on the ideXlab platform.
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the effects of feeding with synbiotic pediococcus acidilactici and fructooligosaccharide enriched adult Artemia on skin mucus immune responses stress resistance intestinal microbiota and performance of angelfish pterophyllum scalare
Fish & Shellfish Immunology, 2016Co-Authors: Mahmood Azimirad, Saeed Meshkini, Nasrollah Ahmadifard, Seyed Hossein HoseinifarAbstract:Abstract The aim of this study was to evaluate the effects of feeding on synbiotic ( Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish ( Pterophyllum scalare ). Three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 L) assigned to four groups in triplicates. Fish were fed for 7 weeks with dietary treatments, including treatment 1: feeding adult Artemia without enrichment (control group), treatment 2: feeding adult Artemia enriched with lyophilised probiotic P. acidilactici (700 mg L −1 ), 3: feeding adult Artemia enriched with prebiotic fructooligosaccharide (FOS) (100 mg L −1 ), group 4: feeding adult Artemia enriched with synbiotic ( P. acidilactici (700 mg L −1 ) + FOS (100 mg L −1 )). Skin mucus immune responses (lysozyme activity, total Immunoglobulin and protease), stress resistance against environmental stress (acute decrease of temperature and increase salinity), intestinal microbiota as well as growth indices were measured at the end of feeding trial. Artemia enriched with synbiotic significantly improved growth performance compared to other treatments ( P P P Artemia enriched with synbiotic ( P angelfish with synbiotic enriched Artemia was more effective than singular enrichment with probiotics or prebiotics.
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the effects of feeding with synbiotic pediococcus acidilactici and fructooligosaccharide enriched adult Artemia on skin mucus immune responses stress resistance intestinal microbiota and performance of angelfish pterophyllum scalare
Fish & Shellfish Immunology, 2016Co-Authors: Mahmood Azimirad, Saeed Meshkini, Nasrollah Ahmadifard, Seyed Hossein HoseinifarAbstract:The aim of this study was to evaluate the effects of feeding on synbiotic (Pediococcus acidilactici and fructooligosaccharide) enriched adult Artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish (Pterophyllum scalare). Three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 L) assigned to four groups in triplicates. Fish were fed for 7 weeks with dietary treatments, including treatment 1: feeding adult Artemia without enrichment (control group), treatment 2: feeding adult Artemia enriched with lyophilised probiotic P. acidilactici (700 mg L(-1)), 3: feeding adult Artemia enriched with prebiotic fructooligosaccharide (FOS) (100 mg L(-1)), group 4: feeding adult Artemia enriched with synbiotic (P. acidilactici (700 mg L(-1)) + FOS (100 mg L(-1))). Skin mucus immune responses (lysozyme activity, total Immunoglobulin and protease), stress resistance against environmental stress (acute decrease of temperature and increase salinity), intestinal microbiota as well as growth indices were measured at the end of feeding trial. Artemia enriched with synbiotic significantly improved growth performance compared to other treatments (P < 0.05). The highest weight gain and specific growth rate (SGR) was observed in synbiotic fed fish (P < 0.05). Compared to the other treatments, the population of lactic acid bacteria was significantly higher in the intestinal microbiota of fish fed synbiotic supplemented diet (P < 0.05). In the environmental stress challenge test, the maximum resistance to abrupt decrease of temperature (17 °C) or elevation of salinity (12 g per liter) was observed in the synbiotic treatment. Also, the total immunoglobulin and lysozyme activity level of skin mucus was significantly elevated in fish fed Artemia enriched with synbiotic (P < 0.05). These results revealed that feeding angelfish with synbiotic enriched Artemia was more effective than singular enrichment with probiotics or prebiotics.
Thomas H Macrae - One of the best experts on this subject based on the ideXlab platform.
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Reproductive Biology of Artemia
Artemia: Basic and Applied Biology, 2020Co-Authors: Godelieve R. J. Criel, Thomas H MacraeAbstract:The genus, Artemia, consists of bisexual and parthenogenetic strains. In North America, only bisexual species are found, whereas in Europe, Asia and Africa, bisexual and parthenogenetic populations occur. Females of most Artemia strains reproduce ovoviviparously and oviparously, releasing either nauplius larvae or encysted embryos, respectively (Jackson and Clegg, 1996; Liang and MacRae, 1999) (Figure 1). Reproductive mode may switch, in good rearing conditions Artemia tend to reproduce ovoviviparously, whereas under adverse situations they reproduce oviparously. Females differ in their genetic tendency to reproduce either ovoviviparously or oviparously.
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Stress tolerance during diapause and quiescence of the brine shrimp, Artemia
Cell Stress and Chaperones, 2016Co-Authors: Thomas H MacraeAbstract:Oviparously developing embryos of the brine shrimp, Artemia , arrest at gastrulation and are released from females as cysts before entering diapause, a state of dormancy and stress tolerance. Diapause is terminated by an external signal, and growth resumes if conditions are permissible. However, if circumstances are unfavorable, cysts enter quiescence, a dormant stage that continues as long as adverse conditions persist. Artemia embryos in diapause and quiescence are remarkably resistant to environmental and physiological stressors, withstanding desiccation, cold, heat, oxidation, ultraviolet radiation, and years of anoxia at ambient temperature when fully hydrated. Cysts have adapted to stress in several ways; they are surrounded by a rigid cell wall impermeable to most chemical compounds and which functions as a shield against ultraviolet radiation. Artemia cysts contain large amounts of trehalose, a non-reducing sugar thought to preserve membranes and proteins during desiccation by replacing water molecules and/or contributing to vitrification. Late embryogenesis abundant proteins similar to those in seeds and other anhydrobiotic organisms are found in cysts, and they safeguard cell organelles and proteins during desiccation. Artemia cysts contain abundant amounts of p26, a small heat shock protein, and artemin, a ferritin homologue, both ATP-independent molecular chaperones important in stress tolerance. The evidence provided in this review supports the conclusion that it is the interplay of these protective elements that make Artemia one of the most stress tolerant of all metazoan organisms.
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priming the prophenoloxidase system of Artemia franciscana by heat shock proteins protects against vibrio campbellii challenge
Fish & Shellfish Immunology, 2011Co-Authors: Kartik Baruah, Thomas H Macrae, Patrick Sorgeloos, Jayant Ranjan, Peter BossierAbstract:Abstract Like other invertebrates, the brine shrimp Artemia franciscana relies solely on innate immunity, which by definition lacks adaptive characteristics, to combat against invading pathogens. One of the innate mechanisms is melanisation of bacteria mediated by the activation of the prophenoloxidase (proPO) system. The 70 kDa heat shock proteins (Hsp70) derived from either prokaryote ( Escherichia coli ) or eukaryote ( Artemia ), well conserved and immune-dominant molecules, protect Artemia against Vibrio campbellii . However, the molecular mechanisms by which these proteins protect Artemia against Vibrio campbellii infection are unknown. Here we demonstrated that feeding gnotobiotically grown Artemia with either Artemia Hsp70 or the E. coli Hsp70 equivalent DnaK, each overproduced in E. coli , followed by V. campbellii challenge enhanced the proPO system, at both mRNA and protein activity levels. Additionally, the Artemia fed with these proteins survived well in a Vibrio challenge assay. These results indicated that Hsp70s derived from either prokaryotic or eukaryotic sources generate protective immunity in the crustacean Artemia against V. campbellii infection by priming the proPO system. This is apparently the first in vivo report on priming activity of Hsp70 in an invertebrate.
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functional characterization of artemin a ferritin homolog synthesized in Artemia embryos during encystment and diapause
FEBS Journal, 2007Co-Authors: Tania S Villeneuve, Tao Chen, Katy Garant, Reinout Amons, Thomas H MacraeAbstract:Oviparously developing embryos of the crustacean Artemia franciscana encyst and enter diapause, exhibiting a level of stress tolerance seldom seen in metazoans. The extraordinary stress resistance of encysted Artemia embryos is thought to depend in part on the regulated synthesis of artemin, a ferritin superfamily member. The objective of this study was to better understand artemin function, and to this end the protein was synthesized in Escherichia coli and purified to apparent homogeneity. Purified artemin consisted of oligomers approximately 700 kDa in molecular mass that dissociated into monomers and a small number of dimers upon SDS/PAGE. Artemin inhibited heat-induced aggregation of citrate synthase in vitro, an activity characteristic of molecular chaperones and shown here to be shared by apoferritin and ferritin. This is the first report that apoferritin/ferritin may protect cells from stress other than by iron sequestration. Stably transfected mammalian cells synthesizing artemin were more resistant to heat and H2O2 than were cells transfected with vector only, actions also shared by molecular chaperones such as the small heat shock proteins. The data indicate that artemin is a structurally modified ferritin arising either from a common ancestor gene or by duplication of the ferritin gene. Divergence, including acquisition of a C-terminal peptide extension and ferroxidase center modification, eliminated iron sequestration, but chaperone activity was retained. Therefore, because artemin accumulates abundantly during development, it has the potential to protect embryos from stress during encystment and diapause without adversely affecting iron metabolism.
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molecular chaperones stress resistance and development in Artemia franciscana
Seminars in Cell & Developmental Biology, 2003Co-Authors: Thomas H MacraeAbstract:Abstract Embryos of the brine shrimp, Artemia franciscana , either develop directly into swimming larvae or are released from females as encysted gastrulae (cysts) which enter diapause, a reversible state of dormancy. Metabolic activity in diapause cysts is very low and these embryos are remarkably resistant to physiological stresses. Encysting embryos, but not those undergoing uninterrupted development, synthesize large amounts of two proteins, namely p26 and artemin. Cloning and sequencing demonstrated p26 is a small heat shock/α-crystallin protein while artemin has structural similarity to ferritin. p26 exhibits molecular chaperone activity in vitro, moves reversibly into nuclei during stress and confers thermotolerance on transformed organisms, suggesting critical roles in cyst development. The function of artemin is unknown. Encysted Artemia also contain an abundance of trehalose, a disaccharide capable of protecting embryos. Artemia represent a novel experimental system where the developmental functions of small heat shock/α-crystallin proteins and other stress response elements can be explored.
