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Venkataraman Subramanian - One of the best experts on this subject based on the ideXlab platform.
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evaluation of antiarthritic activity of strychnos potatorum linn seeds in freund s adjuvant induced arthritic rat model
BMC Complementary and Alternative Medicine, 2010Co-Authors: Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Venkataraman SubramanianAbstract:Strychnos potatorum Linn (Loganiaceae) is a moderate sized tree found in southern and central parts of India, Sri Lanka and Burma. In traditional system of medicine, Strychnos potatorum Linn seeds were used for various ailments including inflammation, diabetes etc. To investigate the folkloric use of the seeds the present study was carried out on Freund's adjuvant induced arthritic rats. The present study states the effect of the aqueous extract (SPE) and the whole seed powder (SPP) of Strychnos potatorum Linn seeds on the Freund's complete adjuvant (FCA) induced arthritic rat paw edema, body weight changes and alterations in haematological and biochemical parameters in both developing and developed phases of Arthritis. Histopathology of proximal interphalangeal joints and radiology of hind legs were studied. In FCA induced arthritic rats, there was significant increase in rat paw volume and decrease in body weight increment, whereas SPP and SPE treated groups, showed significant reduction in paw volume and normal gain in body weight. The altered haematological parameters (Hb, RBC, WBC and ESR) and biochemical parameters (blood urea, serum creatinine, total proteins and acute phase proteins) in the arthritic rats were significantly brought back to near normal by the SPP and SPE treatment at the dose of 200 mg/kg/p.o in both developing and developed phases of Arthritis. Further the histopathological and radiological studies revealed the antiarthritic activity of SPP and SPE by indicating fewer abnormalities in these groups when compared to the arthritic control group. In conclusion, both SPP and SPE at the specified dose level of 200 mg/kg, p.o. showed reduction in rat paw edema volume and it could significantly normalize the haematological and biochemical abnormalities in adjuvant induced arthritic rats in both developing and developed phases of FCA induced Arthritis. Further the histopathological and radiological studies confirmed the antiarthritic activity of SPP and SPE.
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evaluation of antiarthritic activity of strychnos potatorum linn seeds in freund s adjuvant induced arthritic rat model
BMC Complementary and Alternative Medicine, 2010Co-Authors: Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Venkataraman SubramanianAbstract:Strychnos potatorum Linn (Loganiaceae) is a moderate sized tree found in southern and central parts of India, Sri Lanka and Burma. In traditional system of medicine, Strychnos potatorum Linn seeds were used for various ailments including inflammation, diabetes etc. To investigate the folkloric use of the seeds the present study was carried out on Freund's adjuvant induced arthritic rats. The present study states the effect of the aqueous extract (SPE) and the whole seed powder (SPP) of Strychnos potatorum Linn seeds on the Freund's complete adjuvant (FCA) induced arthritic rat paw edema, body weight changes and alterations in haematological and biochemical parameters in both developing and developed phases of Arthritis. Histopathology of proximal interphalangeal joints and radiology of hind legs were studied. In FCA induced arthritic rats, there was significant increase in rat paw volume and decrease in body weight increment, whereas SPP and SPE treated groups, showed significant reduction in paw volume and normal gain in body weight. The altered haematological parameters (Hb, RBC, WBC and ESR) and biochemical parameters (blood urea, serum creatinine, total proteins and acute phase proteins) in the arthritic rats were significantly brought back to near normal by the SPP and SPE treatment at the dose of 200 mg/kg/p.o in both developing and developed phases of Arthritis. Further the histopathological and radiological studies revealed the antiarthritic activity of SPP and SPE by indicating fewer abnormalities in these groups when compared to the arthritic control group. In conclusion, both SPP and SPE at the specified dose level of 200 mg/kg, p.o. showed reduction in rat paw edema volume and it could significantly normalize the haematological and biochemical abnormalities in adjuvant induced arthritic rats in both developing and developed phases of FCA induced Arthritis. Further the histopathological and radiological studies confirmed the antiarthritic activity of SPP and SPE.
R.l. Stevens - One of the best experts on this subject based on the ideXlab platform.
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mast cells contribute to autoimmune inflammatory Arthritis via their tryptase heparin complexes
Journal of Immunology, 2009Co-Authors: Ki-chul Shin, Patrick H Mcneil, Katherine Larabee, Roberto Adachi, Peter A. Nigrovic, James F Crish, Michael F. Gurish, Eric Boilard, Reuben Gobezie, R.l. StevensAbstract:Although mast cells (MCs) often are abundant in the synovial tissues of patients with rheumatoid Arthritis, the contribution of MCs to joint inflammation and cartilage loss remains poorly understood. MC-restricted tryptase/heparin complexes have proinflammatory activity, and significant amounts of human tryptase β (hTryptase-β) are present in rheumatoid Arthritis synovial fluid. Mouse MC protease-6 (mMCP-6) is the ortholog of hTryptase-β, and this serine protease is abundant in the synovium of arthritic mice. We now report that C57BL/6 (B6) mice lacking their tryptase/heparin complexes have attenuated arthritic responses, with mMCP-6 as the dominant tryptase responsible for augmenting neutrophil infiltration in the K/BxN mouse serum-transfer Arthritis model. While inflammation in this experimental Arthritis model was not dependent on protease-activated receptor-2, it was dependent on the chemokine receptor CXCR2. In support of the latter data, exposure of synovial fibroblasts to hTryptase-β/heparin or mMCP-6/heparin complexes resulted in expression of the neutrophil chemotactic factors CXCL1/KC, CXCL5/LIX, and CXCL8/IL-8. Our proteomics, histochemistry, and immunohistochemistry data also revealed substantial loss of cartilage-derived aggrecan proteoglycans in the arthritic joints of wild-type B6 mice but not mMCP-6-null B6 mice. These observations demonstrate the functional contribution of MC-restricted tryptase/heparin complexes in the K/BxN mouse Arthritis model and connect our mouse findings with rheumatoid Arthritis pathophysiology.
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mast cells contribute to autoimmune inflammatory Arthritis via their tryptase heparin complexes
Journal of Immunology, 2009Co-Authors: Ki-chul Shin, Patrick H Mcneil, Katherine Larabee, Roberto Adachi, Peter A. Nigrovic, James F Crish, Michael F. Gurish, Eric Boilard, Reuben Gobezie, R.l. StevensAbstract:Although mast cells (MCs) often are abundant in the synovial tissues of patients with rheumatoid Arthritis, the contribution of MCs to joint inflammation and cartilage loss remains poorly understood. MC-restricted tryptase/heparin complexes have proinflammatory activity, and significant amounts of human tryptase beta (hTryptase-beta) are present in rheumatoid Arthritis synovial fluid. Mouse MC protease-6 (mMCP-6) is the ortholog of hTryptase-beta, and this serine protease is abundant in the synovium of arthritic mice. We now report that C57BL/6 (B6) mice lacking their tryptase/heparin complexes have attenuated arthritic responses, with mMCP-6 as the dominant tryptase responsible for augmenting neutrophil infiltration in the K/BxN mouse serum-transfer Arthritis model. While inflammation in this experimental Arthritis model was not dependent on protease-activated receptor-2, it was dependent on the chemokine receptor CXCR2. In support of the latter data, exposure of synovial fibroblasts to hTryptase-beta/heparin or mMCP-6/heparin complexes resulted in expression of the neutrophil chemotactic factors CXCL1/KC, CXCL5/LIX, and CXCL8/IL-8. Our proteomics, histochemistry, and immunohistochemistry data also revealed substantial loss of cartilage-derived aggrecan proteoglycans in the arthritic joints of wild-type B6 mice but not mMCP-6-null B6 mice. These observations demonstrate the functional contribution of MC-restricted tryptase/heparin complexes in the K/BxN mouse Arthritis model and connect our mouse findings with rheumatoid Arthritis pathophysiology.
Sanmuga Priya Ekambaram - One of the best experts on this subject based on the ideXlab platform.
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evaluation of antiarthritic activity of strychnos potatorum linn seeds in freund s adjuvant induced arthritic rat model
BMC Complementary and Alternative Medicine, 2010Co-Authors: Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Venkataraman SubramanianAbstract:Strychnos potatorum Linn (Loganiaceae) is a moderate sized tree found in southern and central parts of India, Sri Lanka and Burma. In traditional system of medicine, Strychnos potatorum Linn seeds were used for various ailments including inflammation, diabetes etc. To investigate the folkloric use of the seeds the present study was carried out on Freund's adjuvant induced arthritic rats. The present study states the effect of the aqueous extract (SPE) and the whole seed powder (SPP) of Strychnos potatorum Linn seeds on the Freund's complete adjuvant (FCA) induced arthritic rat paw edema, body weight changes and alterations in haematological and biochemical parameters in both developing and developed phases of Arthritis. Histopathology of proximal interphalangeal joints and radiology of hind legs were studied. In FCA induced arthritic rats, there was significant increase in rat paw volume and decrease in body weight increment, whereas SPP and SPE treated groups, showed significant reduction in paw volume and normal gain in body weight. The altered haematological parameters (Hb, RBC, WBC and ESR) and biochemical parameters (blood urea, serum creatinine, total proteins and acute phase proteins) in the arthritic rats were significantly brought back to near normal by the SPP and SPE treatment at the dose of 200 mg/kg/p.o in both developing and developed phases of Arthritis. Further the histopathological and radiological studies revealed the antiarthritic activity of SPP and SPE by indicating fewer abnormalities in these groups when compared to the arthritic control group. In conclusion, both SPP and SPE at the specified dose level of 200 mg/kg, p.o. showed reduction in rat paw edema volume and it could significantly normalize the haematological and biochemical abnormalities in adjuvant induced arthritic rats in both developing and developed phases of FCA induced Arthritis. Further the histopathological and radiological studies confirmed the antiarthritic activity of SPP and SPE.
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evaluation of antiarthritic activity of strychnos potatorum linn seeds in freund s adjuvant induced arthritic rat model
BMC Complementary and Alternative Medicine, 2010Co-Authors: Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Venkataraman SubramanianAbstract:Strychnos potatorum Linn (Loganiaceae) is a moderate sized tree found in southern and central parts of India, Sri Lanka and Burma. In traditional system of medicine, Strychnos potatorum Linn seeds were used for various ailments including inflammation, diabetes etc. To investigate the folkloric use of the seeds the present study was carried out on Freund's adjuvant induced arthritic rats. The present study states the effect of the aqueous extract (SPE) and the whole seed powder (SPP) of Strychnos potatorum Linn seeds on the Freund's complete adjuvant (FCA) induced arthritic rat paw edema, body weight changes and alterations in haematological and biochemical parameters in both developing and developed phases of Arthritis. Histopathology of proximal interphalangeal joints and radiology of hind legs were studied. In FCA induced arthritic rats, there was significant increase in rat paw volume and decrease in body weight increment, whereas SPP and SPE treated groups, showed significant reduction in paw volume and normal gain in body weight. The altered haematological parameters (Hb, RBC, WBC and ESR) and biochemical parameters (blood urea, serum creatinine, total proteins and acute phase proteins) in the arthritic rats were significantly brought back to near normal by the SPP and SPE treatment at the dose of 200 mg/kg/p.o in both developing and developed phases of Arthritis. Further the histopathological and radiological studies revealed the antiarthritic activity of SPP and SPE by indicating fewer abnormalities in these groups when compared to the arthritic control group. In conclusion, both SPP and SPE at the specified dose level of 200 mg/kg, p.o. showed reduction in rat paw edema volume and it could significantly normalize the haematological and biochemical abnormalities in adjuvant induced arthritic rats in both developing and developed phases of FCA induced Arthritis. Further the histopathological and radiological studies confirmed the antiarthritic activity of SPP and SPE.
Thomas Kamradt - One of the best experts on this subject based on the ideXlab platform.
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b cell depletion reduces the number of autoreactive t helper cells and prevents glucose 6 phosphate isomerase induced Arthritis
PLOS ONE, 2011Co-Authors: Oliver Frey, Lisa Bruns, Lars Morawietz, Kyri Dunussijoannopoulos, Thomas KamradtAbstract:The therapeutic benefit of B cell depletion in patients with rheumatoid Arthritis has provided proof of concept that B cells are relevant for the pathogenesis of Arthritis. It remains unknown which B cell effector functions contribute to the induction or chronification of Arthritis. We studied the clinical and immunological effects of B cell depletion in glucose-6-phosphate isomerase-induced Arthritis. We targeted CD22 to deplete B cells. Mice were depleted of B cells before or after immunization with glucose-6-phosphate isomerase (G6PI). The clinical and histological effects were studied. G6PI-specific antibody responses were measured by ELISA. G6PI-specific T helper (Th) cell responses were assayed by polychromatic flow cytometry. B cell depletion prior to G6PI-immunization prevented Arthritis. B cell depletion after immunization ameliorated Arthritis, whereas B cell depletion in arthritic mice was ineffective. Transfer of antibodies from arthritic mice into B cell depleted recipients did not reconstitute Arthritis. B cell depleted mice harbored much fewer G6PI-specific Th cells than control animals. B cell depletion prevents but does not cure G6PI-induced Arthritis. Arthritis prevention upon B cell depletion is associated with a drastic reduction in the number of G6PI-specific effector Th cells.
Ki-chul Shin - One of the best experts on this subject based on the ideXlab platform.
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mast cells contribute to autoimmune inflammatory Arthritis via their tryptase heparin complexes
Journal of Immunology, 2009Co-Authors: Ki-chul Shin, Patrick H Mcneil, Katherine Larabee, Roberto Adachi, Peter A. Nigrovic, James F Crish, Michael F. Gurish, Eric Boilard, Reuben Gobezie, R.l. StevensAbstract:Although mast cells (MCs) often are abundant in the synovial tissues of patients with rheumatoid Arthritis, the contribution of MCs to joint inflammation and cartilage loss remains poorly understood. MC-restricted tryptase/heparin complexes have proinflammatory activity, and significant amounts of human tryptase β (hTryptase-β) are present in rheumatoid Arthritis synovial fluid. Mouse MC protease-6 (mMCP-6) is the ortholog of hTryptase-β, and this serine protease is abundant in the synovium of arthritic mice. We now report that C57BL/6 (B6) mice lacking their tryptase/heparin complexes have attenuated arthritic responses, with mMCP-6 as the dominant tryptase responsible for augmenting neutrophil infiltration in the K/BxN mouse serum-transfer Arthritis model. While inflammation in this experimental Arthritis model was not dependent on protease-activated receptor-2, it was dependent on the chemokine receptor CXCR2. In support of the latter data, exposure of synovial fibroblasts to hTryptase-β/heparin or mMCP-6/heparin complexes resulted in expression of the neutrophil chemotactic factors CXCL1/KC, CXCL5/LIX, and CXCL8/IL-8. Our proteomics, histochemistry, and immunohistochemistry data also revealed substantial loss of cartilage-derived aggrecan proteoglycans in the arthritic joints of wild-type B6 mice but not mMCP-6-null B6 mice. These observations demonstrate the functional contribution of MC-restricted tryptase/heparin complexes in the K/BxN mouse Arthritis model and connect our mouse findings with rheumatoid Arthritis pathophysiology.
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mast cells contribute to autoimmune inflammatory Arthritis via their tryptase heparin complexes
Journal of Immunology, 2009Co-Authors: Ki-chul Shin, Patrick H Mcneil, Katherine Larabee, Roberto Adachi, Peter A. Nigrovic, James F Crish, Michael F. Gurish, Eric Boilard, Reuben Gobezie, R.l. StevensAbstract:Although mast cells (MCs) often are abundant in the synovial tissues of patients with rheumatoid Arthritis, the contribution of MCs to joint inflammation and cartilage loss remains poorly understood. MC-restricted tryptase/heparin complexes have proinflammatory activity, and significant amounts of human tryptase beta (hTryptase-beta) are present in rheumatoid Arthritis synovial fluid. Mouse MC protease-6 (mMCP-6) is the ortholog of hTryptase-beta, and this serine protease is abundant in the synovium of arthritic mice. We now report that C57BL/6 (B6) mice lacking their tryptase/heparin complexes have attenuated arthritic responses, with mMCP-6 as the dominant tryptase responsible for augmenting neutrophil infiltration in the K/BxN mouse serum-transfer Arthritis model. While inflammation in this experimental Arthritis model was not dependent on protease-activated receptor-2, it was dependent on the chemokine receptor CXCR2. In support of the latter data, exposure of synovial fibroblasts to hTryptase-beta/heparin or mMCP-6/heparin complexes resulted in expression of the neutrophil chemotactic factors CXCL1/KC, CXCL5/LIX, and CXCL8/IL-8. Our proteomics, histochemistry, and immunohistochemistry data also revealed substantial loss of cartilage-derived aggrecan proteoglycans in the arthritic joints of wild-type B6 mice but not mMCP-6-null B6 mice. These observations demonstrate the functional contribution of MC-restricted tryptase/heparin complexes in the K/BxN mouse Arthritis model and connect our mouse findings with rheumatoid Arthritis pathophysiology.
