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Arthroderma benhamiae

The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform

Takashi Mochizuki – 1st expert on this subject based on the ideXlab platform

  • Mating Type Gene (MAT1-2) of Trichophyton verrucosum
    Mycopathologia, 2014
    Co-Authors: Rui Kano, Atsuhiko Hasegawa, Takashi Mochizuki, Kazushi Anzawa, Erina Yoshida, Takashi Yaguchi, Vit Hubka, Hiroshi Kamata

    Abstract:

    Trichophyton verrucosum is a zoophilic dermatophyte species that is the most frequent etiologic agent of bovine dermatophytosis throughout the world. Since no teleomorph of T. verrucosum has been found, polymerase chain reaction (PCR) analysis on the genome of T. verrucosum isolated from the Czech Republic and Japan was performed to confirm the presence of a mating type locus in the genome of the fungus and to clarify its classification and ecological characteristics. The mating type gene ( MAT1 – 2) allele was detected by PCR analysis in all 22 isolates (four isolates from the Czech Republic and 18 isolates from Japan). The nucleotide sequence of the region exhibited 99–100 % identity among all isolates, including the reference strain of T. verrucosum . Phylogenetic analysis revealed that the sequences of the internal transcribed spacer region at the MAT1 – 2 locus clustered together in the isolates examined, forming a branch distinct from that of the other dermatophyte species. These results suggest that T. verrucosum is a clonal offshoot that has drifted away from Arthroderma benhamiae.

  • molecular epidemiology of a major subgroup of Arthroderma benhamiae isolated in japan by restriction fragment length polymorphism analysis of the non transcribed spacer region of ribosomal rna gene
    Japanese Journal of Infectious Diseases, 2012
    Co-Authors: Kiminobu Takeda, Kazushi Anzawa, Akiko Nishibu, Takashi Mochizuki

    Abstract:

    SUMMARY: Arthroderma benhamiae vectored by small animals, such as household pets, causes tinea lesions on human skin. The number of tinea cases caused by this species is increasing in Japan. We attempted to develop a simple molecular method for strain discrimination, which is expected to be useful in molecular epidemiology. Out of the 61 strains of A. benhamiae registered at our institute, 46 A. benhamiae strains showed very high degrees of sequence similarity on cluster analysis of the internal transcribed spacer regions of ribosomal RNA (rRNA) genes. These 46 strains, including 22 strains isolated from Japan, were further used for strain typing by analyzing the non-transcribed spacer (NTS) region of the rRNA gene. Polymerase chain reaction was performed using a primer pair designed for amplification of a part of the NTS region, and the amplicons were successfully discriminated by restriction fragment length polymorphism (RFLP) analysis performed using MvaI. RFLP analysis showed 11 NTS types (NTS1–NTS11) among the 46 strains. Out of the 22 Japanese strains, 10 were of the NTS8 type; 6, of the NTS1 type; 3, of the NTS2 type; and 3, of the NTS5 type. Molecular typing showed consistency of NTS types among the strains isolated from different lesions on the same patient, among the strains derived from the same family, and among the strains from pets and their owners. We observed that 3 out of the 4 NTS types among the Japanese strains were detected outside Japan as well.

  • Arthroderma benhamiae the teleomorph of trichophyton mentagrophytes mating type specific genes
    Mycopathologia, 2011
    Co-Authors: Rui Kano, Koichi Makimura, Tsuyoshi Yamada, Masako Kawasaki, Takashi Mochizuki, Hiroshi Kamata, Atsuhiko Hasegawa

    Abstract:

    This study first report to identify the mating type (−)-specific gene of alpha-box and the mating type (+)-specific gene of the high-mobility-group (HMG) DNA-binding domain in zoophilic dermatophytes of Arthroderma benhamiae in an effort to understand the epidemiological characteristics of Trichophyton mentagrophytes. The sequence of the alpha-box gene (1,387 bp) was found to contain two exons, from 184 to 475 bp and from 525 to 1,387 bp, coding a protein of 384 amino acids, beginning with a putative initiating methionine (ATG). The sequence of the HMG gene (1,910 bp) contained two exons, from 234 to 415 bp and from 479 to 1,457 bp, coding a protein of 386 amino acids, beginning with a putative initiating methionine (ATG).

Atsuhiko Hasegawa – 2nd expert on this subject based on the ideXlab platform

  • Occurrence of Arthroderma benhamiae Genotype in Japan
    Mycopathologia, 2015
    Co-Authors: Junichiro Hiruma, Masataro Hiruma, Rui Kano, Atsuhiko Hasegawa, Michel Monod, Kazutoshi Harada, Ryoji Tsuboi

    Abstract:

    In this study, we epidemiologically investigated on clinical isolates of Arthroderma benhamiae from humans and animals in Japan by internal transcribed spacer (ITS) region sequence analysis and mating type ( MAT )-specific PCR. Seven of 8 A. benhamiae isolates from a human, rabbits and guinea pigs were identified as group I (white phenotype) by morphological characters and ITS region sequence analysis. One strain isolated from a degus ( Octodon degus) produced colonies with few irregular folds and yellow velvety mycelium without macro- and microconidia. This strain resembled to group II (yellow phenotype) strain. ITS sequence analysis was also 100 % identical to that of group II. MAT -specific PCR indicated that 6 of these 7 isolates of group I contained an alpha-box gene and that one strain contained high-mobility-group ( HMG ) gene. One strain of group II was revealed to have an alpha-box gene and no HMG gene. To our knowledge, it is the first A. benhamiae isolate of group II found in Japan. The A. benhamiae may be more widespread in worldwide than our surpassing what is common or usual or expected.

  • Mating Type Gene (MAT1-2) of Trichophyton verrucosum
    Mycopathologia, 2014
    Co-Authors: Rui Kano, Atsuhiko Hasegawa, Takashi Mochizuki, Kazushi Anzawa, Erina Yoshida, Takashi Yaguchi, Vit Hubka, Hiroshi Kamata

    Abstract:

    Trichophyton verrucosum is a zoophilic dermatophyte species that is the most frequent etiologic agent of bovine dermatophytosis throughout the world. Since no teleomorph of T. verrucosum has been found, polymerase chain reaction (PCR) analysis on the genome of T. verrucosum isolated from the Czech Republic and Japan was performed to confirm the presence of a mating type locus in the genome of the fungus and to clarify its classification and ecological characteristics. The mating type gene ( MAT1 – 2) allele was detected by PCR analysis in all 22 isolates (four isolates from the Czech Republic and 18 isolates from Japan). The nucleotide sequence of the region exhibited 99–100 % identity among all isolates, including the reference strain of T. verrucosum . Phylogenetic analysis revealed that the sequences of the internal transcribed spacer region at the MAT1 – 2 locus clustered together in the isolates examined, forming a branch distinct from that of the other dermatophyte species. These results suggest that T. verrucosum is a clonal offshoot that has drifted away from Arthroderma benhamiae.

  • Arthroderma benhamiae the teleomorph of trichophyton mentagrophytes mating type specific genes
    Mycopathologia, 2011
    Co-Authors: Rui Kano, Koichi Makimura, Tsuyoshi Yamada, Masako Kawasaki, Takashi Mochizuki, Hiroshi Kamata, Atsuhiko Hasegawa

    Abstract:

    This study first report to identify the mating type (−)-specific gene of alpha-box and the mating type (+)-specific gene of the high-mobility-group (HMG) DNA-binding domain in zoophilic dermatophytes of Arthroderma benhamiae in an effort to understand the epidemiological characteristics of Trichophyton mentagrophytes. The sequence of the alpha-box gene (1,387 bp) was found to contain two exons, from 184 to 475 bp and from 525 to 1,387 bp, coding a protein of 384 amino acids, beginning with a putative initiating methionine (ATG). The sequence of the HMG gene (1,910 bp) contained two exons, from 234 to 415 bp and from 479 to 1,457 bp, coding a protein of 386 amino acids, beginning with a putative initiating methionine (ATG).

Rui Kano – 3rd expert on this subject based on the ideXlab platform

  • Cutaneous mycoses in Japan originating from animals
    Medical mycology journal, 2020
    Co-Authors: Rui Kano

    Abstract:

    : Human cases of dermatophytoses are occasionally transmitted from animals, and suffered from tinea corporis and sometimes Kerion celsi. The most frequent causative agent of these diseases is Microsporum canis. The other dermatophyte, Arthroderma benhamiae is now prevailing in rabbits, rodents and hedgehogs that are popular household pets in Japan. Therefore, some human cases of A. benhamiae infection were reported and the transmission of this infection from rabbits and rodents was confirmed.Cryptococcosis is regarded as dangerous zoonosis, but its transmission from animal to peoples has not been documented in Japan. Animal cases of cryptococcosis are possible to increase in number by developing immunosuppressive animals as well as by spreading of newly introduced C. gattii to Japan.Animal cases of sporotrichosis are rarely reported in Japan. However, feline sporotrichosis should be prevented and promptly treated since it easily transmitted to people from cat lesions and the exudates where copious numbers of organisms are found in tissues.

  • Occurrence of Arthroderma benhamiae Genotype in Japan
    Mycopathologia, 2015
    Co-Authors: Junichiro Hiruma, Masataro Hiruma, Rui Kano, Atsuhiko Hasegawa, Michel Monod, Kazutoshi Harada, Ryoji Tsuboi

    Abstract:

    In this study, we epidemiologically investigated on clinical isolates of Arthroderma benhamiae from humans and animals in Japan by internal transcribed spacer (ITS) region sequence analysis and mating type ( MAT )-specific PCR. Seven of 8 A. benhamiae isolates from a human, rabbits and guinea pigs were identified as group I (white phenotype) by morphological characters and ITS region sequence analysis. One strain isolated from a degus ( Octodon degus) produced colonies with few irregular folds and yellow velvety mycelium without macro- and microconidia. This strain resembled to group II (yellow phenotype) strain. ITS sequence analysis was also 100 % identical to that of group II. MAT -specific PCR indicated that 6 of these 7 isolates of group I contained an alpha-box gene and that one strain contained high-mobility-group ( HMG ) gene. One strain of group II was revealed to have an alpha-box gene and no HMG gene. To our knowledge, it is the first A. benhamiae isolate of group II found in Japan. The A. benhamiae may be more widespread in worldwide than our surpassing what is common or usual or expected.

  • tinea faciei caused by trichophyton mentagrophytes molecular type Arthroderma benhamiae mimics impetigo a case report and literature review of cases in japan
    Medical mycology journal, 2015
    Co-Authors: Utako Kimura, Masataro Hiruma, Rui Kano, Kae Yokoyama, Kenji Takamori, Yasushi Suga

    Abstract:

    : A 36-year-old female elementary schoolteacher presented with aggregated serous papules surrounded by mild erythema, extending from both nasal wings/nostrils down to the upper lip. No improvement was seen following treatment of the lesions with topical antibiotics for impetigo. Potassium hydroxide (KOH) direct microscopy confirmed the presence of mycelia, and the infection was diagnosed as tinea faciei. The isolate was identified as Trichophyton mentagrophytes using morphological analysis and as Arthroderma benhamiae using genetic analysis. Here we describe that case and summarize the clinical features of other cases of A. benhamiae infection in Japan that have been reported in the literature.