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S. Murakami - One of the best experts on this subject based on the ideXlab platform.

  • SEROLOGICAL STUDY OF RADIATION HAZARD. II. THE EFFECT OF X-RAY TO PASSIVE SKIN ALLERGY
    2020
    Co-Authors: S. Murakami
    Abstract:

    White albumin, dissolved in potassium alum solution in a concentration of 1.5 mg/ml was used as antigen, and was injected into the ear vein of a rabbit every other day for 12 times. The whole blood was removed on the 7th day following the last injection, and the separated blood serum was kept in an ice box after the addition of merzonin in the proportion of 1: 10000. The irradiated guinea pigs were injected with the rabbit's serum and were injected again with various concentrations of antigen into the abdominal skin after 24 hr. The skin reaction was examined a day later. After sensitization, 1.5 ml of 0.5% Evans blue solution per kg in weight was injected and immediately 0.1 ml of 1.0, 0.1, 0.01, and 0.001% of antigen was injected subcutaneous in the abdomen, 30 min after which the state of skin reaction was studied. Arthus Phenomenon was mostly inhibited on the 7th day after 200 r of x irradiation, and was gradually recovered and showed slightly increased tendency on the 28th day. The area of skin reaction in Arthus Phenomenon was minimum on the day following 200 r and showed increased tendency on the 28th day. After 400more » r the Arthus Phenomenon was mostly inhibited on the 7th day and the area of skin reaction became almost absent. Arthus Phenomenon indicated by the Evans blue method showed a maximum area of skin reaction on the 7th day after 200 r, which was diffusely infiltrated with an unsharp margin and then gradually became small and sharply demarcated on the 28th day. The histological changes of the skin in the guinea pig following 200 r were slight with atrophy of the epithelium and no other changes seen. (Absts. Japan. Med., 1: No. 7, 1960.)« less

Hideyo Yamaguchi - One of the best experts on this subject based on the ideXlab platform.

  • suppressive effects of lanoconazole on Arthus Phenomenon in vivo and on production and functions of tnf in vitro
    Nippon Ishinkin Gakkai Zasshi, 2000
    Co-Authors: Masayasu Mitsuya, Kayoko Wada, Hiroko Ishibashi, Shigeru Tansho, Hideyo Yamaguchi
    Abstract:

    抗真菌剤ラノコナゾール(LCZ)の抗炎症作用をin vivoとin vitro実験で検討した.卵白アルブミンで感作されたウサギでアルツス現象を惹起し,1および2%に含むLCZクリーム剤を反応局所へ塗布し,反応惹起開始から炎症の終了まで肉眼的に観察した.抗原を注入した局所の浮腫,紅斑,出血および壊死の反応サイズを測定した結果,2%LCZ剤塗布が出血抑制効果を示すことが解った.一方,炎症性サイトカインであるTNFの産生とその機能に対するLCZの効果をin vitro実験で検討した.LCZは腹腔マクロファージのTNF産生を20μg/mlで抑制し,ヒト末梢血の好中球の粘着反応を100μg/mlで抑制した.さらに,LCZはTNFによるL-929繊維芽細胞増殖抑制作用を0.05μg/mlで抑制した.このことはLCZが極めて低濃度でサイトカインによる細胞毒性から繊維芽細胞を防護する可能性を示唆する.以上の結果はLCZが炎症反応に対する抑制効果をもつことを示すとともに,その作用が細胞保護効果と関連している可能性を示唆している.

V. A. Frolov - One of the best experts on this subject based on the ideXlab platform.

Aliyeva Tarana Rzakuli - One of the best experts on this subject based on the ideXlab platform.

  • determination of levels of ige and igg and the activity of complement comparatively in blood and lymph in the experimental Arthus and overy Phenomenon
    International Journal of Immunology, 2019
    Co-Authors: Aliyeva Tarana Rzakuli
    Abstract:

    The aim of investigation was the determination of the levels of IgE, IgG and complement activity in the blood and lymph in the experimental Arthus and Overy Phenomenon. Experiments were carried out on 27 chinchilla rabbits (weight 2.5-3kg) in three series. To reproduce the Arthus Phenomenon, rabbits were sensitized by subcutaneous administration of 1 ml of horse serum to the scapular region every 5 days; after the fifth injection in the injection area of horse serum the necrosis was observed. To obtain the Overy Phenomenon, a resolving dose of horse serum was injected subcutaneously in a sensitized animal, and an Evans blue was introduced into the auricular vein. In this case, the injection area is colored blue. The blood necessary for the experiment was taken from the marginal auricular vein of the rabbit, and lymph – from the thoracic lymphatic duct. The levels of IgE and IgG and the activity of the complement comparatively in the blood and lymph in the experimental Arthus and Overy Phenomenon were studied. The experiments were carried out on 27 rabbits of the «Chinchilla» breed in two series. The studied parameters of IgE and IgG and complement activity in blood and lymph of intact animals served as control. The results of the study showed that the level of IgG in the Arthus Phenomenon increases and IgE decreases. While at the Overy Phenomenon, on the contrary, the level of IgE increases and the level of IgG decreases. The titer of complement in immune complex reactions is sharply reduced; it indicates the involvement of complement with IgG elimination of the allergen.

Filatov Oy - One of the best experts on this subject based on the ideXlab platform.

  • Phenomenon of specific inhibition of luminol-dependent chemiluminescence of polymorphonuclear leukocytes under the influence of allergen.
    Journal of Investigational Allergology and Clinical Immunology, 1996
    Co-Authors: Pytsky Vi, Filatov Oy
    Abstract:

    : The purpose of our investigation was to establish how the intensity of induced luminol-dependent chemiluminescence of polymorphonuclear leukocytes is affected by the cultivation of allergen with the blood of allergic patients (pollinosis, bronchial asthma and penicillin allergy) and animals with allergic processes (Arthus' Phenomenon, guinea pigs sensitized by horse serum). The results of all assays were uniform. In all cases three phases of changes in induced chemiluminescence were established. In the first phase with the minimum doses of specific and unspecific allergens, either no changes in chemiluminescence were found or chemiluminescence even increased. With higher doses of allergen came the second phase - inhibition of stimulated chemiluminescence when specific allergen had been added and the absence of changes in the tests with unspecific allergen. We defined this phase as the phase of specific chemiluminescence inhibition. With a further increase in the concentration of allergen came the third phase. It expressed itself in the inhibition of stimulated chemiluminescence under the influence of both specific and unspecific allergen. We viewed this phase as a manifestation of the toxic influence of allergen on polymorphonuclear leukocytes. We suggested using the second phase for diagnosing sensitization.