The Experts below are selected from a list of 3933 Experts worldwide ranked by ideXlab platform
Kenneth G. Gould - One of the best experts on this subject based on the ideXlab platform.
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Functional parameters of chimpanzee (Pan troglodytes) sperm from ejaculates collected by rectal probe electrostimulation and by Artificial Vagina.
American journal of primatology, 1996Co-Authors: Kenneth G. Gould, Leona G. YoungAbstract:This study compares functional parameters of sperm from ejaculates collected from 15 adult male chimpanzees using rectal probe electrostimulation (RPE) and from 10 adult male chimpanzees trained to use an Artificial Vagina (AV). Computer assisted motion analysis (CAMA) showed no significant differences in mean values for straight line velocity (VSL), linearity (LIN), curvilinear velocity (VCL), and lateral head movement (ALH) of sperm from ejaculates collected by RPE and by AV. There was, however, a significant difference (P < 0.01) in the population distribution for VSL and LIN, which indicates that sperm swim in a more convoluted manner in ejaculates collected by RPE than in ejaculates collected by AV. In the hamster zona-free ovum penetration assay (SPA), there were no significant differences in the percentages of hamster oocytes penetrated by sperm or in the number of sperm which penetrated each oocyte after 4 or 24 h incubation using sperm from ejaculates collected by RPE and by AV. Therefore, the lack of success using sperm from ejaculates collected by RPE to initiate pregnancy in the chimpanzee does not appear to result from abnormalities in sperm fertilizing capacity as measured in SPA. © 1996 Wiley-Liss, Inc.
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Characteristics of Chimpanzee (Pan troglodytes) Ejaculates Collected by Rectal Probe Electrostimulation and by Artificial Vagina
American journal of primatology, 1995Co-Authors: Leona G. Young, Eleanor B. Smithwick, Kenneth G. GouldAbstract:This study compares characteristics of ejaculates collected from 16 adult male chimpanzees using rectal probe electrostimulation (RPE) and from 10 adult male chimpanzees trained to use an Artificial Vagina (AV). Ejaculate weight, semen volume, and sperm number were significantly lower (P < 0.01) and percentage liquefaction was significantly higher (P < 0.01) in ejaculates collected by RPE. Percentages of motile sperm and of live sperm in semen did not differ significantly between the two collection methods. Total amounts of protein and of α-glucosidase activity were significantly lower (P < 0.01) in seminal fluid from RPE samples. For ejaculates collected by RPE, semen volume correlated positively with protein (r = 0.8640, P < 0.001), fructose (r = 0.6976, P < 0.001), and citrate (r = 0.6976, P < 0.001); sperm number correlated positively with α-glucosidase activity (r = 0.6547, P < 0.001); and protein correlated positively with fructose (r = 0.5906, P < 0.002), citrate (r = 0.5926, P < 0.002) and α-glucosidase activity (r = 0.6006, P < 0.001). For ejaculates collected by AV, semen volume correlated positively with percentage liquefaction (r = 0.6058, P < 0.001), protein (r = 0.8055, P < 0.001), fructose (r = 0.6606, P < 0.001), and citrate (r = 0.8272, P < 0.001); sperm number correlated positively with percentage of motile sperm (r = 0.4196, P 0.004); percentage of motile sperm correlated positively with percentage of live sperm (r = 0.4388, P < 0.002); and, protein correlated positively with fructose (r = 0.6947, P < 0.002) and with citrate (r = 0.5926, P < 0.002). These data show that there is a significant difference in semen parameters and in biochemical parameters of ejaculates obtained by RPE and by AV. © 1995 Wiley-Liss, Inc.
Leona G. Young - One of the best experts on this subject based on the ideXlab platform.
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Functional parameters of chimpanzee (Pan troglodytes) sperm from ejaculates collected by rectal probe electrostimulation and by Artificial Vagina.
American journal of primatology, 1996Co-Authors: Kenneth G. Gould, Leona G. YoungAbstract:This study compares functional parameters of sperm from ejaculates collected from 15 adult male chimpanzees using rectal probe electrostimulation (RPE) and from 10 adult male chimpanzees trained to use an Artificial Vagina (AV). Computer assisted motion analysis (CAMA) showed no significant differences in mean values for straight line velocity (VSL), linearity (LIN), curvilinear velocity (VCL), and lateral head movement (ALH) of sperm from ejaculates collected by RPE and by AV. There was, however, a significant difference (P < 0.01) in the population distribution for VSL and LIN, which indicates that sperm swim in a more convoluted manner in ejaculates collected by RPE than in ejaculates collected by AV. In the hamster zona-free ovum penetration assay (SPA), there were no significant differences in the percentages of hamster oocytes penetrated by sperm or in the number of sperm which penetrated each oocyte after 4 or 24 h incubation using sperm from ejaculates collected by RPE and by AV. Therefore, the lack of success using sperm from ejaculates collected by RPE to initiate pregnancy in the chimpanzee does not appear to result from abnormalities in sperm fertilizing capacity as measured in SPA. © 1996 Wiley-Liss, Inc.
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Characteristics of Chimpanzee (Pan troglodytes) Ejaculates Collected by Rectal Probe Electrostimulation and by Artificial Vagina
American journal of primatology, 1995Co-Authors: Leona G. Young, Eleanor B. Smithwick, Kenneth G. GouldAbstract:This study compares characteristics of ejaculates collected from 16 adult male chimpanzees using rectal probe electrostimulation (RPE) and from 10 adult male chimpanzees trained to use an Artificial Vagina (AV). Ejaculate weight, semen volume, and sperm number were significantly lower (P < 0.01) and percentage liquefaction was significantly higher (P < 0.01) in ejaculates collected by RPE. Percentages of motile sperm and of live sperm in semen did not differ significantly between the two collection methods. Total amounts of protein and of α-glucosidase activity were significantly lower (P < 0.01) in seminal fluid from RPE samples. For ejaculates collected by RPE, semen volume correlated positively with protein (r = 0.8640, P < 0.001), fructose (r = 0.6976, P < 0.001), and citrate (r = 0.6976, P < 0.001); sperm number correlated positively with α-glucosidase activity (r = 0.6547, P < 0.001); and protein correlated positively with fructose (r = 0.5906, P < 0.002), citrate (r = 0.5926, P < 0.002) and α-glucosidase activity (r = 0.6006, P < 0.001). For ejaculates collected by AV, semen volume correlated positively with percentage liquefaction (r = 0.6058, P < 0.001), protein (r = 0.8055, P < 0.001), fructose (r = 0.6606, P < 0.001), and citrate (r = 0.8272, P < 0.001); sperm number correlated positively with percentage of motile sperm (r = 0.4196, P 0.004); percentage of motile sperm correlated positively with percentage of live sperm (r = 0.4388, P < 0.002); and, protein correlated positively with fructose (r = 0.6947, P < 0.002) and with citrate (r = 0.5926, P < 0.002). These data show that there is a significant difference in semen parameters and in biochemical parameters of ejaculates obtained by RPE and by AV. © 1995 Wiley-Liss, Inc.
Eleanor B. Smithwick - One of the best experts on this subject based on the ideXlab platform.
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Characteristics of Chimpanzee (Pan troglodytes) Ejaculates Collected by Rectal Probe Electrostimulation and by Artificial Vagina
American journal of primatology, 1995Co-Authors: Leona G. Young, Eleanor B. Smithwick, Kenneth G. GouldAbstract:This study compares characteristics of ejaculates collected from 16 adult male chimpanzees using rectal probe electrostimulation (RPE) and from 10 adult male chimpanzees trained to use an Artificial Vagina (AV). Ejaculate weight, semen volume, and sperm number were significantly lower (P < 0.01) and percentage liquefaction was significantly higher (P < 0.01) in ejaculates collected by RPE. Percentages of motile sperm and of live sperm in semen did not differ significantly between the two collection methods. Total amounts of protein and of α-glucosidase activity were significantly lower (P < 0.01) in seminal fluid from RPE samples. For ejaculates collected by RPE, semen volume correlated positively with protein (r = 0.8640, P < 0.001), fructose (r = 0.6976, P < 0.001), and citrate (r = 0.6976, P < 0.001); sperm number correlated positively with α-glucosidase activity (r = 0.6547, P < 0.001); and protein correlated positively with fructose (r = 0.5906, P < 0.002), citrate (r = 0.5926, P < 0.002) and α-glucosidase activity (r = 0.6006, P < 0.001). For ejaculates collected by AV, semen volume correlated positively with percentage liquefaction (r = 0.6058, P < 0.001), protein (r = 0.8055, P < 0.001), fructose (r = 0.6606, P < 0.001), and citrate (r = 0.8272, P < 0.001); sperm number correlated positively with percentage of motile sperm (r = 0.4196, P 0.004); percentage of motile sperm correlated positively with percentage of live sperm (r = 0.4388, P < 0.002); and, protein correlated positively with fructose (r = 0.6947, P < 0.002) and with citrate (r = 0.5926, P < 0.002). These data show that there is a significant difference in semen parameters and in biochemical parameters of ejaculates obtained by RPE and by AV. © 1995 Wiley-Liss, Inc.
E.y. Kwawukume - One of the best experts on this subject based on the ideXlab platform.
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Construction of an Artificial Vagina with sigmoid colon in Vaginal agenesis.
International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics, 1994Co-Authors: T.s. Ghosh, E.y. KwawukumeAbstract:OBJECTIVES: The study of 15 cases of colonic reconstruction for absent Vagina. The outcome in cases of reconstruction of an art@cial Vagina using the colon is evaluated. METHODS: Fifteen female patients with congenital absence of Vagina who were evaluated and treated with sigmoid colonic reconstruction at the Korle-Bu Teaching Hospital in Accra, Ghana were studied. RESULTS: This reconstructive method is successful in patients having a functioning Vagina without progressive shrinkage or other symptoms. Disfigurement of the thighs and labia are avoided as is the needfor active manipulation. CONCLUSION: Sigmoid colonic reconstruction for Vaginal agenesis is an acceptable method especially in the developing world. of Vaginal dilatation was successful in 9 out of 21 patients. Other workers reported various successes [4,5] and the main advantage of dilatation is the simplicity of the method [4], but this technique requires active manipulation by the patient or the obstetrician.
Terttu Katila - One of the best experts on this subject based on the ideXlab platform.
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quality of stallion semen obtained by a new semen collection phantom equidame versus a missouri Artificial Vagina
Theriogenology, 1999Co-Authors: Heli Lindeberg, Heidi Karjalainen, Erkki Koskinen, Terttu KatilaAbstract:A study was performed to test a new semen collection device (Equidame® phantom) that fractionates the ejaculate by comparing the quality of semen obtained by the Equidame® phantom with that obtained by a Missouri® Artificial Vagina. Semen from 4 Finnhorse stallions was collected 4 times per stallion by both methods. Half of the ejaculate was frozen and the other half extended and loaded into 2 Equitainer® transport containers (24- and 48-h samples). Motility parameters were determined by a Hamilton-Thorn motility analyzer after cooled storage for 24 and 48 h and again after freezing/thawing. Raw and chilled semen samples were cultured and the number of bacterial colonies counted after incubations of 24 and 48 h. After a 24-h incubation the number of colony-forming units (CFU) in raw semen was significantly higher (P<0.01) when collected by the Missouri® Artificial Vagina than by the Equidame® phantom. After cooled storage, 75% of the semen samples contained no bacteria after an incubation of 24 h, and 69% yielded no growth after 48 h. The sperm-rich fractions (Cup 2) collected by the Equidame® phantom had lower mean volumes (22.1 ± 2.3 mL [± SEM] versus 101.6 ± 9.3 mL) and significantly higher mean sperm concentrations (218.0 ± 25.8 × 106 vs 86.2 ± 8.1 × 106 cells/mL; P<0.05) than the total ejaculates collected by the Missouri® device. The total and progressive motility of chilled and frozen-thawed semen did not differ significantly between collection methods. The Equidame® phantom yielded semen that was of a lower bacteriological colony counts, but had sperm motility similar to that of semen collected with the traditional method by the Missouri® Artificial Vagina.
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Quality of stallion semen obtained by a new semen collection phantom (Equidame) versus a Missouri Artificial Vagina
Theriogenology, 1999Co-Authors: Heli Lindeberg, Heidi Karjalainen, Erkki Koskinen, Terttu KatilaAbstract:A study was performed to test a new semen collection device (Equidame® phantom) that fractionates the ejaculate by comparing the quality of semen obtained by the Equidame® phantom with that obtained by a Missouri® Artificial Vagina. Semen from 4 Finnhorse stallions was collected 4 times per stallion by both methods. Half of the ejaculate was frozen and the other half extended and loaded into 2 Equitainer® transport containers (24- and 48-h samples). Motility parameters were determined by a Hamilton-Thorn motility analyzer after cooled storage for 24 and 48 h and again after freezing/thawing. Raw and chilled semen samples were cultured and the number of bacterial colonies counted after incubations of 24 and 48 h. After a 24-h incubation the number of colony-forming units (CFU) in raw semen was significantly higher (P
