Aseptic Conditions

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Pilar Hernández-muñoz - One of the best experts on this subject based on the ideXlab platform.

  • Preservation of Aseptic Conditions in absorbent pads by using silver nanotechnology
    Food Research International, 2009
    Co-Authors: Avelina Fernández, Pierre Picouet, Eva Soriano, Gracia López-carballo, Elsa Lloret, Rafael Gavara, Pilar Hernández-muñoz
    Abstract:

    Silver nanoparticles have been formed in fluff pulp and nanostructured Lyocell fibres by immersion in silver nitrate, and a subsequent transformation of the adsorbed silver ions into elementary silver nanoparticles by physical (thermal/UV) or chemical (sodium borohydride) methods. Microscopy revealed that nanoparticles generated by physical methods were regular in shape and efficiently dispersed, while the chemical reduction produced highly aggregated nanoparticles. Nanoparticle size has been found relevant to guarantee high antimicrobial activity, being the samples with big aggregated silver nanoparticles almost inefficient. Indeed a satisfactory correlation between silver ion release and the antimicrobial efficiency against Escherichia coli and Staphylococcus aureus could be confirmed, and furthermore, the highest concentrations tested were efficient to reduce the microbial load in poultry exudates. This work demonstrates that especially designed absorbent materials could be optimised to preserve Aseptic Conditions during manipulation, leading to feasible applications of a silver based nanotechnology in food technology.

María Alejandra Alvarez - One of the best experts on this subject based on the ideXlab platform.

S. Poncet - One of the best experts on this subject based on the ideXlab platform.

Avelina Fernández - One of the best experts on this subject based on the ideXlab platform.

  • Preservation of Aseptic Conditions in absorbent pads by using silver nanotechnology
    Food Research International, 2009
    Co-Authors: Avelina Fernández, Pierre Picouet, Eva Soriano, Gracia López-carballo, Elsa Lloret, Rafael Gavara, Pilar Hernández-muñoz
    Abstract:

    Silver nanoparticles have been formed in fluff pulp and nanostructured Lyocell fibres by immersion in silver nitrate, and a subsequent transformation of the adsorbed silver ions into elementary silver nanoparticles by physical (thermal/UV) or chemical (sodium borohydride) methods. Microscopy revealed that nanoparticles generated by physical methods were regular in shape and efficiently dispersed, while the chemical reduction produced highly aggregated nanoparticles. Nanoparticle size has been found relevant to guarantee high antimicrobial activity, being the samples with big aggregated silver nanoparticles almost inefficient. Indeed a satisfactory correlation between silver ion release and the antimicrobial efficiency against Escherichia coli and Staphylococcus aureus could be confirmed, and furthermore, the highest concentrations tested were efficient to reduce the microbial load in poultry exudates. This work demonstrates that especially designed absorbent materials could be optimised to preserve Aseptic Conditions during manipulation, leading to feasible applications of a silver based nanotechnology in food technology.

Kyle W. Hollman - One of the best experts on this subject based on the ideXlab platform.

  • HIGH FREQUENCY ULTRASONIC IMAGING OF GROWTH AND DEVELOPMENT IN MANUFACTURED ENGINEERED ORAL MUCOSAL TISSUE SURFACES
    Ultrasound in medicine & biology, 2014
    Co-Authors: Frank Winterroth, Hiroko Kato, Shiuhyang Kuo, Stephen E. Feinberg, Scott J. Hollister, J. Brian Fowlkes, Kyle W. Hollman
    Abstract:

    This study uses high-resolution ultrasound to examine the growth and development of engineered oral mucosal tissues manufactured under Aseptic Conditions. The specimens are a commercially available natural tissue scaffold, AlloDerm, and oral keratinocytes seeded onto AlloDerm to form an ex vivo-produced oral mucosal equivalent (EVPOME) suitable for intra-oral grafting. The seeded cells produce a keratinized protective upper layer that smooths out any remaining surface irregularities on the underlying AlloDerm. Two-dimensional acoustic imaging of unseeded AlloDerm and developing EVPOMEs was performed on each day of their growth and development, each tissue specimen being imaged under Aseptic Conditions (total time from seeding to maturation: 11 d). Ultrasonic monitoring offers us the ability to determine the constituents of the EVPOME that are responsible for changes in its mechanical behavior during the manufacturing process. Ultrasonic monitoring affords us an opportunity to non-invasively assess, in real time, tissue-engineered constructs before release for use in patient care.