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Indrawati Roostantia - One of the best experts on this subject based on the ideXlab platform.
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Standarisasi Ekstrak Etanol Centella Asiatica dengan Identifikasi Kandungan Asiaticoside, Asiatic Acid dan Madecasic Acid
Bagian Biokimia Fakultas Kedokteran Universitas Gajah Mada, 2015Co-Authors: Mustika Arifa, Indrawati RoostantiaAbstract:Ekstrak etanol centella asiatica iuga dibuktikan mampu memperbaiki kerusakan jaringan pada paru tikus yang diinfeksi dengan Mycobacterium tuberculosis. Ekstrak etanol 'Centella asiatica menurunkan matrix metalloproteinase-1 dan meningkatkan ti ssue inhibitor of metalloproteinase pada jaringan paru tikus yang diinfeksi Mycobacterium tubercurosis. Data ini menunjukkan bahwa ekstrak etanol Centella aslatica berpotensi untuk menjadi obat fitofarmaka. Uituk menjadikan ekstrak etanol Centella asratica sebagai obat fitofarmaka perlu dilakukan standariiasi kandungan bahan aKif dari ekstrak tersebut, yaitu Asiaticoside, 'asiatic acid dan madecasid acid. Oleh karena itu, pada penelitian ini dilakukan standarisasi sediaan ekstrak dengan identifikasi bahan aktif yaitu asiaticosde, asiatic acid dan madecasid acid . ldentifikasi bahan aktif di dalam ekstrak dilakukan dengan menggunakan kromatografi lapis tipis densitometri dan dibandingkan dengan standar. Fase gerak yang digunakan terdiri dari campuran etil asetat: metanol: aquadest dengan perbandingan 10:2,5:1 ,5; untuk pemeriksaan Asiaticoside. Fase gerak untuk pemeriksaan Asiatic acid dan Madecasid acid ierdiri dari campuran etil asetat: Metanol: Aqua dengan perbandingan 10; 2,5:0,5. Hasil penelitian menunjukkan waktu tambat standar Asiaticoside 0,55 sedangkan ekstrak Cenfella asiatica O,54, standar Asiatic acid 1 ,06 sedangkan ekstrak Cenfella asiatica sebesar 1,05, standar madecasid acid 1,03 sedangkan ekstrak centella asiatica 1 ,05. Kesimpulan dari penelitian ini adalah ekstrak etanol Centella asiatica mengandung Asiaticoside, asiatic acid dan madecasic aci
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Standarisasi Ekstrak Etanol Centella asiatica dengan Identifikasi Kandungan Asiaticoside, Asiatic acid dan Madecasic acid
Bagian Biokimia Fakultas Kedokteran Universitas Gajah Mada, 2015Co-Authors: Mustika Arifa, Indrawati RoostantiaAbstract:Ekstrak etanol centella asiatica juga dibuktikan mampu memperbaiki kerusakan jaringan pada paru tikus yang diinfeksi dengan Mycobacterium tuberculosis. Ekstrak etanol 'Centella asiatica menurunkan matrix metalloproteinase-1 dan meningkatkan ti ssue inhibitor of metalloproteinase pada jaringan paru tikus yang diinfeksi Mycobacterium tuberculosis. Data ini menunjukkan bahwa ekstrak etanol Centella asiatica berpotensi untuk menjadi obat fitofarmaka. Untuk menjadikan ekstrak etanol Centella asiatica sebagai obat fitofarmaka perlu dilakukan standarisasi kandungan bahan aktif dari ekstrak tersebut, yaitu Asiaticoside, 'asiatic acid dan madecasid acid. Oleh karena itu, pada penelitian ini dilakukan standarisasi sediaan ekstrak dengan identifikasi bahan aktif yaitu Asiaticoside, asiatic acid dan madecasid acid . ldentifikasi bahan aktif di dalam ekstrak dilakukan dengan menggunakan kromatografi lapis tipis densitometri dan dibandingkan dengan standar. Fase gerak yang digunakan terdiri dari campuran etil asetat: metanol: aquadest dengan perbandingan 10:2,5:1 ,5; untuk pemeriksaan Asiaticoside. Fase gerak untuk pemeriksaan Asiatic acid dan Madecasid acid ierdiri dari campuran etil asetat: Metanol: Aqua dengan perbandingan 10; 2,5:0,5. Hasil penelitian menunjukkan waktu tambat standar Asiaticoside 0,55 sedangkan ekstrak Cenfel/a asiatica O,54-, standar Asiatic acid 1 ,06 sedangkan ekstrak Cenfel/a aslatica sebesar 1,05, standar madecasid acid 1,03 sedangkan ekstrak centella asiatica 1,05 Kesimpulan dari penelitian ini adalah ekstrak etanol Centella asiatica mengandung Asiaticoside, asiatic acid dan madecasic aci
Zhuo Zhang - One of the best experts on this subject based on the ideXlab platform.
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Asiaticoside attenuates cell growth inhibition and apoptosis induced by aβ1 42 via inhibiting the tlr4 nf κb signaling pathway in human brain microvascular endothelial cells
Frontiers in Pharmacology, 2018Co-Authors: Daqiang Song, Xian Jiang, Yiliu Liu, Yuhong Sun, Shousong Cao, Zhuo ZhangAbstract:Alzheimer's disease (AD) is a very common progressive neurodegenerative disorder with the highest incidence in the world. Dysfunction of the blood-brain barrier (BBB) may be responsible for the pathogenesis and pathology of AD for abnormally transporting amyloid-β (Aβ, the main component of the senile plaques) from the sera into the central nervous system. Aβ peptides induce apoptosis in human brain microvascular endothelial cells (hBMECs), the main component of BBB. Apoptosis in neuronal cells plays a critical role in the pathogenesis of AD. Asiaticoside, a natural glycoside extracted from Centella asiatica (L.) Urban, has an anti-apoptotic effect on hBMECs but the molecule mechanism remains unclear. Therefore, we investigate the protective effect of Asiaticoside on Aβ1-42-induced cytotoxicity and apoptosis as well as associated mechanism in hBMECs with commonly used in vitro methods for clinical development of Asiaticoside as a novel anti-AD agent. In the present study, we investigated the effects of Asiaticoside on cytotoxicity by Cell Counting Kit-8 assay, mitochondrial membrane potential by JC-1 fluorescence analysis, anti-apoptosis by Hoechst 33258 staining and Annexin V-FITC (fluorescein isothiocyanate) and propidium iodide (PI) analyses, the expressions of TNF-α and IL-6 by enzyme-linked immunosorbent assay (ELISA) and TLR4, MyD88, TRAF6, p-NF-κB p65, and total NF-κB p65 by Western blotting, and nuclear translocation of NF-κB p65 by immunofluorescence analysis in hBMECs. The results showed that pretreatment of Asiaticoside (25, 50, and 100 μM) for 12 h significantly attenuated cell growth inhibition and apoptosis, and restored declined mitochondrial membrane potential induced by Aβ1-42 (50 μM) in hBMECs. Asiaticoside also significantly downregulated the elevated expressions of TNF-α, IL-6, TLR4, MyD88, TRAF6, and p-NF-κB p65, as well as inhibited NF-κB p65 translocation from cytoplasm to nucleus induced by Aβ1-42 in hBMECs in a concentration-dependent manner. The possible underlying molecular mechanism of Asiaticoside may be through inhibiting the TLR4/NF-κB signaling pathway. Therefore, Asiaticoside may be developed as a novel agent for the prevention and/or treatment of AD clinically.
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Asiaticoside Attenuates Cell Growth Inhibition and Apoptosis Induced by Aβ1-42 via Inhibiting the TLR4/NF-κB Signaling Pathway in Human Brain Microvascular Endothelial Cells
Frontiers Media S.A., 2018Co-Authors: Daqiang Song, Xian Jiang, Yiliu Liu, Yuhong Sun, Shousong Cao, Zhuo ZhangAbstract:Alzheimer’s disease (AD) is a very common progressive neurodegenerative disorder with the highest incidence in the world. Dysfunction of the blood–brain barrier (BBB) may be responsible for the pathogenesis and pathology of AD for abnormally transporting amyloid-β (Aβ, the main component of the senile plaques) from the sera into the central nervous system. Aβ peptides induce apoptosis in human brain microvascular endothelial cells (hBMECs), the main component of BBB. Apoptosis in neuronal cells plays a critical role in the pathogenesis of AD. Asiaticoside, a natural glycoside extracted from Centella asiatica (L.) Urban, has an anti-apoptotic effect on hBMECs but the molecule mechanism remains unclear. Therefore, we investigate the protective effect of Asiaticoside on Aβ1-42-induced cytotoxicity and apoptosis as well as associated mechanism in hBMECs with commonly used in vitro methods for clinical development of Asiaticoside as a novel anti-AD agent. In the present study, we investigated the effects of Asiaticoside on cytotoxicity by Cell Counting Kit-8 assay, mitochondrial membrane potential by JC-1 fluorescence analysis, anti-apoptosis by Hoechst 33258 staining and Annexin V-FITC (fluorescein isothiocyanate) and propidium iodide (PI) analyses, the expressions of TNF-α and IL-6 by enzyme-linked immunosorbent assay (ELISA) and TLR4, MyD88, TRAF6, p-NF-κB p65, and total NF-κB p65 by Western blotting, and nuclear translocation of NF-κB p65 by immunofluorescence analysis in hBMECs. The results showed that pretreatment of Asiaticoside (25, 50, and 100 μM) for 12 h significantly attenuated cell growth inhibition and apoptosis, and restored declined mitochondrial membrane potential induced by Aβ1-42 (50 μM) in hBMECs. Asiaticoside also significantly downregulated the elevated expressions of TNF-α, IL-6, TLR4, MyD88, TRAF6, and p-NF-κB p65, as well as inhibited NF-κB p65 translocation from cytoplasm to nucleus induced by Aβ1-42 in hBMECs in a concentration-dependent manner. The possible underlying molecular mechanism of Asiaticoside may be through inhibiting the TLR4/NF-κB signaling pathway. Therefore, Asiaticoside may be developed as a novel agent for the prevention and/or treatment of AD clinically
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Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti inflammatory effects and inhibition of the p38 mapk mechanism
Molecular Medicine Reports, 2015Co-Authors: Yang Luo, Zhenyu Wang, Zhuo Zhang, Hongxia Wang, Yi LiuAbstract:Asiaticoside has potent pharmacological activity and broader pharmacological effects, including anti‑oxidant, antidepressant and hepatic protection effects, and the inhibition of tumor cell proliferation. However, the mechanism underlying the effects of Asiaticoside on neurological pain in spinal cord injury (SCI) remain to be fully elucidated. Therefore, the present study investigated the specific mechanism underlying the beneficial action of Asiaticoside in a SCI rat model. In the present study, Basso, Beattie and Bresnahan scores was determined to analyze the therapeutic effects of Asiaticoside on the neurological function of the SCI rat model. The water content of the spinal cord was also determined to measure its effects on the SCI rats. Oxidative stress, levels of nitric oxide and inflammation were detected using commercial kits. Western blot analysis was used to measure the protein expression levels of p38‑mitogen‑activated protein kinase (MAPK) in the SCI rat. Asiaticoside effectively augmented the Basso, Beattie and Bresnahan scores of the SCI rats. Significant reductions in the water content of the spinal cord, the levels of inducible nitric oxide synthase (iNOS), and the activities of the nuclear factor‑κB p65 unit, tumor necrosis factor‑α, interleukin(IL)‑1β and IL‑6 were observed in the experimental animals. Furthermore, on examination of the oxidative stress‑associated parameters, increased production of malondialdehyde and decreased levels of superoxide dismutase, glutathione and glutathione peroxidase were detected in the SCI rat model. Asiaticoside also significantly suppressed the expression of p38‑MAPK, which indicated that the therapeutic effects of Asiaticoside may be associated with the p38‑MAPK pathway. These data confirmed that Asiaticoside attenuates SCI through antioxidant and anti‑inflammatory effects, and through inhibition of the p38‑MAPK mechanism.
Prasit Pavasant - One of the best experts on this subject based on the ideXlab platform.
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Asiaticoside induces osteogenic differentiation of human periodontal ligament cells through the wnt pathway
Journal of Periodontology, 2018Co-Authors: Atika Resti Fitri, Prasit Pavasant, Supakarn Chamni, Piyamas SumrejkanchanakijAbstract:Background Asiaticoside is a compound isolated from Herb Centella asiatica, which has been shown to promote osteogenic differentiation of human periodontal ligament (hPDL) cells. This study investigated the molecular mechanism underlying the Asiaticoside-induced osteogenic differentiation of hPDL cells. Methods hPDL cells were incubated with various concentrations of Asiaticoside to test cell viability by MTT assay. The mRNA expression levels were analyzed by using quantitative real-time polymerase chain reaction (PCR). Osteogenic differentiation was determined by alkaline phosphatase activity assay and alizarin red staining. The subcellular localization of β-catenin was analyzed by both immunofluorescence and western blot. Results The results showed that Asiaticoside had no effect on the cell viability at any of the tested concentrations. Real-time PCR revealed that osterix (OSX) and dentin matrix protein1 (DMP1) mRNA were significantly enhanced by Asiaticoside treatment. Alkaline phosphatase activity and in vitro mineralization were also significantly induced. Interestingly, Asiaticoside dose-dependently increased WNT3A mRNA expression, but not WNT5A and WNT10B. The activation of Wnt signaling was shown to result in nuclear accumulation of β-catenin as evaluated by immunofluorescence staining and western blot analysis. Pre-treatment with recombinant human Dickkopf1 (rhDKK1) inhibited Asiaticoside-induced β-catenin nuclear translocation and osteoblast marker gene expression. Moreover, rhDKK1 attenuated Asiaticoside-induced DMP1 protein expression. Conclusion The data demonstrate that Asiaticoside induces osteogenic differentiation of hPDL cells by activating the Wnt/β-catenin signaling pathway. The findings suggest that Asiaticoside could be used as a novel therapeutic drug for periodontal tissue regeneration.
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Asiaticoside induces type i collagen synthesis and osteogenic differentiation in human periodontal ligament cells
Phytotherapy Research, 2013Co-Authors: Nunthawan Nowwarote, Thanaphum Osathanon, Peachaya Jitjaturunt, Sukuman Manopattanasoontorn, Prasit PavasantAbstract:Asiaticoside, an active ingredient extracted from Centella asiatica, has been widely used to promote wound healing. In this study, the effects of Asiaticoside on proliferation, protein synthesis, and osteogenic differentiation in human periodontal ligament cells (HPDLs) were investigated. HPDLs were treated with Asiaticoside at concentrations of 25, 50, and 100 µg/mL. Cell number was determined by MTT assay. The mRNA expression was analyzed by reverse transcription-polymerase chain reaction. Western blot analysis and immunocytochemistry were used to confirm protein synthesis. Osteogenic differentiation was determined by alkaline phosphatase activity, osteoblast marker gene expression, and in vitro mineralization. The results showed that Asiaticoside treatment, ranging from 25 to 100 mg/mL, had no effect on cytotoxicity or cell proliferation. When HPDLs were treated with Asiaticoside in serum-free medium, dose-dependent increases in the levels of fibronectin and collagen type I mRNA and protein were observed at 72 h. Moreover, Asiaticoside attenuated matrix metalloproteinase-1 but enhanced tissue inhibitor of metalloproteinase-1 mRNA expression. The addition of Asiaticoside to osteogenic medium resulted in an increase in alkaline phosphatase enzymatic activity, up-regulation of osteoblast marker gene mRNA expression, and enhancement of mineralization by HPDLs. These results suggest the potential application of Asiaticoside for enhancing periodontal tissue healing.
A Zuraini - One of the best experts on this subject based on the ideXlab platform.
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Asiaticoside inhibits tnf α induced endothelial hyperpermeability of human aortic endothelial cells
Phytotherapy Research, 2015Co-Authors: Lai Yen Fong, Zainul Amiruddin Zakaria, Mohamad Taufik Hidayat Baharuldin, A K Arifah, Muhammad Nazrul Hakim, A ZurainiAbstract:The increase in endothelial permeability often promotes edema formation in various pathological conditions. Tumor necrosis factor-alpha (TNF-α), a pro-atherogenic cytokine, impairs endothelial barrier function and causes endothelial dysfunction in early stage of atherosclerosis. Asiaticoside, one of the triterpenoids derived from Centella asiatica, is known to possess antiinflammatory activity. In order to examine the role of Asiaticoside in preserving the endothelial barrier, we assessed its effects on endothelial hyperpermeability and disruption of actin filaments evoked by TNF-α in human aortic endothelial cells (HAEC). TNF-α caused an increase in endothelial permeability to fluorescein isothiocyanate (FITC)-dextran. Asiaticoside pretreatment significantly suppressed TNF-α-induced increased permeability. Asiaticoside also prevented TNF-α-induced actin redistribution by suppressing stress fiber formation. However, the increased F to G actin ratio stimulated by TNF-α was not changed by Asiaticoside. Cytochalasin D, an actin depolymerizing agent, was used to correlate the anti-hyperpermeability effect of Asiaticoside with actin cytoskeleton. Surprisingly, Asiaticoside failed to prevent cytochalasin D-induced increased permeability. These results suggest that Asiaticoside protects against the disruption of endothelial barrier and actin rearrangement triggered by TNF-α without a significant change in total actin pool. However, Asiaticoside seems to work by other mechanisms to maintain the integrity of endothelial barrier rather than stabilizing the F-actin organization.
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Asiaticoside inhibits TNF-alpha-induced endothelial hyperpermeability of human aortic endothelial cells
'Wiley', 2015Co-Authors: Lai Yen Fong, Zakaria, Zainul Amiruddin, A K Arifah, Theng Ng Chin, Baharuldin, Mohamad Taufik Hidayat, Abdullah, Muhammad Nazrul Hakim, A ZurainiAbstract:The increase in endothelial permeability often promotes edema formation in various pathological conditions. Tumor necrosis factor-alpha (TNF-α), a pro-atherogenic cytokine, impairs endothelial barrier function and causes endothelial dysfunction in early stage of atherosclerosis. Asiaticoside, one of the triterpenoids derived from Centella asiatica, is known to possess antiinflammatory activity. In order to examine the role of Asiaticoside in preserving the endothelial barrier, we assessed its effects on endothelial hyperpermeability and disruption of actin filaments evoked by TNF-α in human aortic endothelial cells (HAEC). TNF-α caused an increase in endothelial permeability to fluorescein isothiocyanate (FITC)-dextran. Asiaticoside pretreatment significantly suppressed TNF-α-induced increased permeability. Asiaticoside also prevented TNF-α-induced actin redistribution by suppressing stress fiber formation. However, the increased F to G actin ratio stimulated by TNF-α was not changed by Asiaticoside. Cytochalasin D, an actin depolymerizing agent, was used to correlate the anti-hyperpermeability effect of Asiaticoside with actin cytoskeleton. Surprisingly, Asiaticoside failed to prevent cytochalasin D-induced increased permeability. These results suggest that Asiaticoside protects against the disruption of endothelial barrier and actin rearrangement triggered by TNF-α without a significant change in total actin pool. However, Asiaticoside seems to work by other mechanisms to maintain the integrity of endothelial barrier rather than stabilizing the F-actin organization
Tursiloadi Silvester - One of the best experts on this subject based on the ideXlab platform.
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STUDI AWAL PENGARUH METODE EKSTRAKSI TERHADAP RENDEMEN DAN KADAR Asiaticoside DARI CENTELLA ASIATICA (L) URB
'National Atomic Energy Agency of Indonesia (BATAN)', 2018Co-Authors: Sondari Dewi, Irawadi, Tun Tedja, Setyaningsih Dwi, Tursiloadi SilvesterAbstract:STUDI AWAL PENGARUH METODE EKSTRAKSI TERHADAP RENDEMEN DAN KADAR Asiaticoside DARI CENTELLA ASIATICA (L) URB. Proses ekstraksi Centella asiatica (L) Urb dengan metode maserasi, sonikasi, sokletasi dan CO2 superkritik telah dilakukan. Pengaruh proses ekstraksi terhadap rendemen dan kadar Asiaticoside dari Centella asiatica (L) Urb (pegagan) telah dipelajari. Hasil dari perhitungan rendemen Asiaticoside terlihat bahwa kandungan Asiaticoside (% berat) dari proses ekstraksimaserasi, sonikasi, sokletasi dan CO2 superkritik berturut-turut sebagai berikut: 6,723%; 0,187%; 3.648%dan 9,24%. Rendemen Asiaticoside paling tinggi diperoleh dari teknologi ekstraksi CO2 superkritik, karena teknologi ini dilakukan pada tekanan dan suhu tertentu sehingga kualitas hasil ekstraksi ditentukan oleh seberapa kritis penggunaan tekanan dan suhunya. Karena pada kondisi ini, selain mengubah densitas CO2, juga berpengaruh terhadap kelarutan dan selektivitas dari zat yang akan terekstrak. Semakin tinggi tekanan dan kelarutan, total hasil ekstraksi akan semakin tinggi. Untukmengetahui adanya senyawa Asiaticoside dalam Centella asiatica (L) Urb (pegagan) digunakan analisis HPLC. Dari hasil analisis kromatogram bahwa ada dua puncak yang terdeteksi, dan secara kualitatif senyawa Asiaticoside yang diperoleh denganmenggunakan ekstraksi CO2 superkritik lebih tinggi kadarnya dibanding metode ekstraksi lainnya, karena teknologi proses ekstraksi CO2 superkritik memanfaatkan kekuatan pelarut dan sifat fisik dari komponenmurni atau campuran, sehinggamudahmelakukan penetrasi ke dalam dinding material yang di ekstrak dan melarutkan komponen senyawa aktif secara selektif dengan kualitas produk tinggi dan tidakmengandung residu pelarut sehingga lebih murni
