The Experts below are selected from a list of 12825 Experts worldwide ranked by ideXlab platform
Bingui Wang - One of the best experts on this subject based on the ideXlab platform.
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antibacterial anthraquinone derivatives isolated from a mangrove derived endophytic fungus Aspergillus nidulans by ethanol stress strategy
The Journal of Antibiotics, 2018Co-Authors: Suiqun Yang, Bingui WangAbstract:Three new natural products, including a new anthraquinone derivative isoversicolorin C (1), a new xanthone analog isosecosterigmatocystin (2), and a new amino acid derivative, glulisine A (3), along with six related metabolites (4-9) were isolated from the culture broth and mycelia extracts of the mangrove-derived endophytic fungus Aspergillus nidulans MA-143 under 0.1% ethanol stress. Their structures were elucidated by detailed analysis of their NMR spectra, ECD spectrum, and X-ray crystallographic experiments. Compounds 1 and 4 showed potent antibacterial activity against some of the tested microbes.
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aniquinazolines a d four new quinazolinone alkaloids from marine derived endophytic fungus Aspergillus nidulans
Marine Drugs, 2013Co-Authors: Minghui Wang, Bingui WangAbstract:Four new quinazolinone alkaloids, namely, aniquinazolines A-D (1-4), were isolated and identified from the culture of Aspergillus nidulans MA-143, an endophytic fungus obtained from the leaves of marine mangrove plant Rhizophora stylosa. The structures of the new compounds were elucidated by spectroscopic analysis, and their absolute configurations were determined on the basis of chiral HPLC analysis of the acidic hydrolysates. The structure for 1 was confirmed by single-crystal X-ray diffraction analysis. All these compounds were examined for antibacterial and cytotoxic activity as well as brine shrimp (Artemia salina) lethality.
Liliane Fraga Costa Ribeiro - One of the best experts on this subject based on the ideXlab platform.
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a novel thermostable xylanase gh10 from malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology
Biotechnology for Biofuels, 2014Co-Authors: Liliane Fraga Costa Ribeiro, Rosymar Coutinho De Lucas, Gabriela Leal Vitcosque, Richard J Ward, Marcelo Ventura Rubio, Andre Damasio, Fabio M Squina, Rebecca C Gregory, Paul H WaltonAbstract:The search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. The present work reports the heterologous expression and biochemical characterization of a novel thermostable xylanase (GH10) from the thermophilic fungus Malbranchea pulchella, the influence of glycosylation on its stability, and a potential application in sugarcane bagasse hydrolysis. Xylanase MpXyn10A was overexpressed in Aspergillus nidulans and was active against birchwood xylan, presenting an optimum activity at pH 5.8 and 80°C. MpXyn10A was 16% glycosylated and thermostable, preserving 85% activity after 24 hours at 65°C, and deglycosylation did not affect thermostability. Circular dichroism confirmed the high alpha-helical content consistent with the canonical GH10 family (β/α)8 barrel fold observed in molecular modeling. Primary structure analysis revealed the existence of eight cysteine residues which could be involved in four disulfide bonds, and this could explain the high thermostability of this enzyme even in the deglycosylated form. MpXyn10A showed promising results in biomass degradation, increasing the amount of reducing sugars in bagasse in natura and in three pretreated sugarcane bagasses. MpXyn10A was successfully secreted in Aspergillus nidulans, and a potential use for sugarcane bagasse biomass degradation was demonstrated.
Minghui Wang - One of the best experts on this subject based on the ideXlab platform.
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aniquinazolines a d four new quinazolinone alkaloids from marine derived endophytic fungus Aspergillus nidulans
Marine Drugs, 2013Co-Authors: Minghui Wang, Bingui WangAbstract:Four new quinazolinone alkaloids, namely, aniquinazolines A-D (1-4), were isolated and identified from the culture of Aspergillus nidulans MA-143, an endophytic fungus obtained from the leaves of marine mangrove plant Rhizophora stylosa. The structures of the new compounds were elucidated by spectroscopic analysis, and their absolute configurations were determined on the basis of chiral HPLC analysis of the acidic hydrolysates. The structure for 1 was confirmed by single-crystal X-ray diffraction analysis. All these compounds were examined for antibacterial and cytotoxic activity as well as brine shrimp (Artemia salina) lethality.
Paul H Walton - One of the best experts on this subject based on the ideXlab platform.
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a novel thermostable xylanase gh10 from malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology
Biotechnology for Biofuels, 2014Co-Authors: Liliane Fraga Costa Ribeiro, Rosymar Coutinho De Lucas, Gabriela Leal Vitcosque, Richard J Ward, Marcelo Ventura Rubio, Andre Damasio, Fabio M Squina, Rebecca C Gregory, Paul H WaltonAbstract:The search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. The present work reports the heterologous expression and biochemical characterization of a novel thermostable xylanase (GH10) from the thermophilic fungus Malbranchea pulchella, the influence of glycosylation on its stability, and a potential application in sugarcane bagasse hydrolysis. Xylanase MpXyn10A was overexpressed in Aspergillus nidulans and was active against birchwood xylan, presenting an optimum activity at pH 5.8 and 80°C. MpXyn10A was 16% glycosylated and thermostable, preserving 85% activity after 24 hours at 65°C, and deglycosylation did not affect thermostability. Circular dichroism confirmed the high alpha-helical content consistent with the canonical GH10 family (β/α)8 barrel fold observed in molecular modeling. Primary structure analysis revealed the existence of eight cysteine residues which could be involved in four disulfide bonds, and this could explain the high thermostability of this enzyme even in the deglycosylated form. MpXyn10A showed promising results in biomass degradation, increasing the amount of reducing sugars in bagasse in natura and in three pretreated sugarcane bagasses. MpXyn10A was successfully secreted in Aspergillus nidulans, and a potential use for sugarcane bagasse biomass degradation was demonstrated.
Michelle Momany - One of the best experts on this subject based on the ideXlab platform.
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Internuclear diffusion of histone H1 within cellular compartments of Aspergillus nidulans
2018Co-Authors: Alexander P. Mela, Michelle MomanyAbstract:Histone H1 is an evolutionarily conserved linker histone protein that functions in arranging and stabilizing chromatin structure and is frequently fused to a fluorescent protein to track nuclei in live cells. In time-lapse analyses, we observed stochastic exchange of photoactivated Dendra2-histone H1 protein between nuclei within the same cellular compartment. We also observed exchange of histones between genetically distinct nuclei in a heterokaryon derived from fusion of strains carrying histone H1-RFP or H1-GFP. Subsequent analysis of the resulting uninucleate conidia containing both RFP- and GFP-labeled histone H1 proteins showed only parental genotypes, ruling out genetic recombination and diploidization. These data together suggest that the linker histone H1 protein can diffuse between non-daughter nuclei in the filamentous fungus Aspergillus nidulans.
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analysis of cell wall sugars in the pathogen Aspergillus fumigatus and the saprophyte Aspergillus nidulans
Mycologia, 2000Co-Authors: Gretel M Guest, Michelle MomanyAbstract:Sugars from cell walls of Aspergillus fumi- gatus and Aspergillus nidulans were analyzed by gas chromatography/mass spectroscopy. The walls of both fungi contain similar levels of glucose, galactose, and mannose. A. fumigatus walls contain 50% less N- acetylglucosamine than A. nidulans walls. In addi- tion, N-acetylgalactosamine is present at low levels in the walls of A. fumigatus and absent in the walls of A. nidulans.
