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Michel Leclerc – One of the best experts on this subject based on the ideXlab platform.
Asterias rubens: Evidence of NF-kappa B genes.Meta gene, 2016Co-Authors: Michel Leclerc, Nicolas Kresdorn, Ralf HorresAbstract:
In the present paper we show a survey of the Asterias rubens sea star genome for genes associated with NF-kappa-B proteins implied in the immune response. The NF-kappa B gene, into 2 subunits, was found in this invertebrate.
The TNFR In Asterias rubens Sea Star: Genomic StudiesJournal of Stem Cell Research & Therapy, 2016Co-Authors: Michel Leclerc, Nicolas KresdornAbstract:
The sea star Asterias rubens includes TNFR, in its genome, when compared to mouse one: mouse isoform 2 of TNF AIP3-interacting protein 1, mouse TNF receptor-associated factor 2, mouse TNF receptor-associated factor 3, mouse TNF receptor-associated factor 4, mouse TNF receptor-associated factor 6. Many of these factors are bound to NF Kappa -B genes which have recently been isolated in the Asterias rubens genome. They play a role in the regulation of the sea star immune system.
a true 34candidate ig kappa gene 34 in the sea star Asterias rubens echinodermaAmerican Journal of Immunology, 2013Co-Authors: Michel Leclerc, Patricia Otten, Magne OsterasAbstract:
The axial organ of the sea-star Asterias rubens is a primitive immune organ. The B-like cells, when stimulated by various antigens, produce antibody substances correlated with Ig Kappa gene. A candidate Ig kappa gene (IgK chain V-IV region S107B precursor) more convincing in term of genome was shown.
Michael C. Thorndyke – One of the best experts on this subject based on the ideXlab platform.
immune suppression of the echinoderm Asterias rubens l following long term ocean acidificationAquatic Toxicology, 2011Co-Authors: Bodil Hernroth, Michael C. Thorndyke, Susanne P Baden, Sam DupontAbstract:
We compared effects of exposure to predict near-future (2100) ocean acidification (OA; pH 7.7) and normal seawater (Control; pH 8.1) on immune and stress responses in the adult sea star Asterias rubens. Analyses were made after one week and after six months of continuous exposure. Following one week exposure to acidified water, the pH of coelomic fluid was significantly reduced. Levels of the chaperon Hsp70 were elevated while key cellular players in immunity, coelomocytes, were reduced by approximately 50%. Following long-term exposure (six months) levels of Hsp70 returned to control values, whereas immunity was further impaired, evidenced by the reduced phagocytic capacity of coelomocytes and inhibited activation of p38 MAP-kinase. Such impacts of reduced seawater pH may have serious consequences for resistance to pathogens in a future acidified ocean.
Functional properties of proteins from the coelomic fluid of the wounded sea star Asterias rubens (L)Journal of invertebrate pathology, 2010Co-Authors: Kristina Holm, Natalia Sharlaimova, Michael C. Thorndyke, I. V. Voronkina, Bodil HernrothAbstract:
Impact on viability and adhesion of three protein fractions, separated by size, from the coelomic fluid of wounded Asterias rubens‘, was tested on autologous coelomocytes. In addition antimicrobial property of the protein fractions was tested on the Gram-negative bacterium Vibrio parahaemolyticus. All fractions promoted viability and the larger proteins facilitated adhesion of the coelomocytes. The strongest antimicrobial effect was caused by the fraction with the smallest proteins.
Tissue Distribution of the SALMFamide Neuropeptides S1 and S2 in the Starfish Asterias rubens Using Novel Monoclonal and Polyclonal Antibodies. I. Nervous and Locomotory SystemsProceedings. Biological sciences, 1995Co-Authors: Suzanna J. Newman, Maurice R. Elphick, Michael C. ThorndykeAbstract:
The recent isolation and characterization of the SALMFamide neuropeptides (S1 and S2) from the starfish Asterias rubens has initiated a series of single- and double-labelling immunocytochemical studies to ascertain their tissue distribution and cellular localization. Specific novel monoclonal and polyclonal antisera have been raised against these neuropeptides and used in optical immunocytochemistry (ICC). The results of the present study reveal, for the first time, the widespread neuronal distribution of S2 localized to axons and perikarya of the radial nerve cord and tube foot ectoneural nerve plexus. Double labelling revealed a predominantly separate localization for S1 and S2 immunoreactivity. The potential functional roles of S1 and S2 in the radial nerve cord (RNC) and tube feet of Asterias rubens are discussed.
P.a. Voogt – One of the best experts on this subject based on the ideXlab platform.
Triacylglycerol lipase activity in the storage organs of the sea star, Asterias rubensComparative Biochemistry and Physiology B, 2010Co-Authors: R.c.h.m. Oudejans, I. Van Der Sluis, P.a. VoogtAbstract:
Abstract 1. 1. In homogenates of pyloric caeca (storage organs) and ovaries of the sea star Asterias rubens lipolytic activity is present toward trioleoylglycerol as the substrate. Most activity was found in the pyloric caeca. 2. 2. Activity of triacylglycerol lipase of pyloric caeca ranged from pH 4 to 10 with maximal activity between pH 6 and 7. 3. 3. Subcellular fractionation revealed highest specific activity of lipase in the 3000 g pellet and highest proportional activity in the 18,000 g supernatant. 4. 4. A cyclic AMP-dependent triacylglycerol lipase was found in the 3000 g pellet. 5. 5. Triacylglycerol lipase activity contributed most to total lipolytic activity, whereas activities of diacylglycerol and monoacylglycerol lipases appeared to be rather low.
Confirmation of the presence of oestradiol-17β in sea star Asterias rubens by GC-MSComparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1992Co-Authors: P.a. Voogt, J.g.d. Lambert, J.c.m. Granneman, M. JansenAbstract:
1. Lipids extracted from the pyloric caeca of female specimens of sea star Asterias rubens were purified extensively by liquid-liquid partition, column chromatography and thin layer chromatography
2. The material from the area on the thin layer plate corresponding with oestradiol-17β was analysed with gas chromatography-mass spectrometry (GC-MS).
3. The retention time corresponded with that of oestradiol-diTMS.
4. SIM analysis showed the six molecular ions and mass fragments characteristic for oestradiol-diTMS to be present in the mass spectrum of the sea star sample at the correct relative abundance ratios.
5. This confirms earlier radioimmunological data.
6. Consequences of this result in relation to the biosynthesis of oestradiol-17β are discussed.
Steroid metabolism in relation to the reproductive cycle in Asterias rubens L.Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1991Co-Authors: P.a. Voogt, P.j. Den Besten, M. JansenAbstract:
1. Conversion rates of dehydroepiandrosterone, progesterone, and androstenedione were studied in gonad and pyloric caeca tissue of female and male sea star, Asterias rubens, at each stage of the reproductive cycle.
2. Each steroid has its own pattern of maximal and minimal conversion rates during the reproductive cycle.
3. The combined patterns of the conversion rates for the three steroids during the reproductive cycle suggest a strict sequence in each organ. These patterns seem to be sex- and organ-specific.
4. Steroid metabolism reaches maximum values at the onset of gonadal growth and at the end of the reproductive cycle. This suggests that steroids are important at least at these phases of the reproductive cycle.