The Experts below are selected from a list of 6561 Experts worldwide ranked by ideXlab platform
Kenneth A Dawson - One of the best experts on this subject based on the ideXlab platform.
-
cationic nanoparticles induce caspase 3 7 and 9 mediated cytotoxicity in a human Astrocytoma Cell line
Nanotoxicology, 2011Co-Authors: Mariana G Bexiga, Juan A Varela, Federico Fenaroli, Fengjuan Wang, Iseult Lynch, Anna Salvati, Jeremy C. Simpson, Kenneth A DawsonAbstract:AbstractOn a daily basis we are exposed to cationic nanoparticulates in many different ways. They are known to distribute to many organs of the body, and while some evidence suggests that these nanoparticles are toxic to Cells, the mechanism of their toxicity is not clear. Here we apply a combination of biochemical and imaging techniques to study the mechanism by which amine-modified polystyrene nanoparticles induce Cell death in a human brain Astrocytoma Cell line. Flow cytometry analysis of Cells exposed to cationic nanoparticles revealed an increase in Cell membrane permeability of the dyes YoPro-1 and propidium iodide, indicating onset of an apoptotic followed by a secondary necrotic response. Activation of caspases 3/7 and 9 and cleavage of poly(ADP-ribose) polymerase (PARP)-1 was also detected, providing clear molecular evidence of the apoptotic pathway induced by the nanoparticles. Transmission electron microscopy also revealed that these nanoparticles induce morphological changes in lysosomes and ...
-
cationic nanoparticles induce caspase 3 7 and 9 mediated cytotoxicity in a human Astrocytoma Cell line
Nanotoxicology, 2011Co-Authors: Mariana G Bexiga, Juan A Varela, Federico Fenaroli, Fengjuan Wang, Iseult Lynch, Anna Salvati, Jeremy C. Simpson, Kenneth A DawsonAbstract:On a daily basis we are exposed to cationic nanoparticulates in many different ways. They are known to distribute to many organs of the body, and while some evidence suggests that these nanoparticles are toxic to Cells, the mechanism of their toxicity is not clear. Here we apply a combination of biochemical and imaging techniques to study the mechanism by which amine-modified polystyrene nanoparticles induce Cell death in a human brain Astrocytoma Cell line. Flow cytometry analysis of Cells exposed to cationic nanoparticles revealed an increase in Cell membrane permeability of the dyes YoPro-1 and propidium iodide, indicating onset of an apoptotic followed by a secondary necrotic response. Activation of caspases 3/7 and 9 and cleavage of poly(ADP-ribose) polymerase (PARP)-1 was also detected, providing clear molecular evidence of the apoptotic pathway induced by the nanoparticles. Transmission electron microscopy also revealed that these nanoparticles induce morphological changes in lysosomes and mitochondria, consistent with our observation of a rapid increase in the formation of reactive oxygen species in these Cells. Together these results suggest that amine-modified polystyrene nanoparticles can mediate Cell death through an apoptotic mechanism mediated by damage to the mitochondria.
Rakesh Shukla - One of the best experts on this subject based on the ideXlab platform.
-
evaluation of amentoflavone isolated from cnestis ferruginea vahl ex dc connaraceae on production of inflammatory mediators in lps stimulated rat Astrocytoma Cell line c6 and thp 1 Cells
Journal of Ethnopharmacology, 2013Co-Authors: Ismaila O Ishola, J P Chaturvedi, Nandhine Rajasekar, Rakesh Shukla, Olufunmilayo O. Adeyemi, Shivika Rai, Thakur NarenderAbstract:Ethnopharmacological relevance Cnestisferruginea (CF) Vahl ex DC (Connaraceae) is a shrub widely used in traditional African medicine for the treatment of various psychiatric illness and inflammatory conditions. Aim of the study This study was carried out to investigate the effect of amentoflavone isolated from methanolic root extract of CF on lipopolysaccharide (LPS)-induced neuroinflammatory cascade of events associated to the oxidative and nitrative stress, and TNF-α production in rat Astrocytoma Cell line (C6) and human monocytic leukemia Cell line (THP-1), respectively. Materials and methods Rat Astrocytoma Cells (C6) were stimulated with LPS (10 μg/ml) alone and in the presence of different concentrations of amentoflavone (0.1–3 μg/ml) for 24 h incubation period. Nitrite release, reactive oxygen species (ROS), malondialdehyde (MDA) and reduced-glutathione (GSH) in C6 Cells were estimated; while the TNF-α level was estimated in THP-1 Cell lysate. In vivo analgesic activity was evaluated using mouse writhing and hot plate tests while the anti-inflammatory effect was investigated using carrageenan-induced oedema test. Results LPS (10 μg/ml) significantly (P<0.05) stimulated C6 Cells to release nitrite, ROS, MDA, and TNF-α generation while GSH was down regulated in comparison to control. However, amentoflavone significantly (P<0.05) attenuated nitrite, ROS, MDA and TNF-α generation and also up regulated the level of GSH. Amentoflavone per se did not have any significant effect on C6 and THP-1 Cells. Amentoflavone (6.25–50 mg/kg) significantly (P<0.05) reduced number of writhes and also increase pain threshold in hot plate test. It produced time course significant (P<0.05) decrease in oedema formation in rodents. Discussion and conclusion Findings in this study demonstrate the anti-neuroinflammatory and antinoceptive effects of amentoflavone which may suggest its beneficial roles in neuroinflammation associated disorders.
-
evaluation of amentoflavone isolated from cnestis ferruginea vahl ex dc connaraceae on production of inflammatory mediators in lps stimulated rat Astrocytoma Cell line c6 and thp 1 Cells
Journal of Ethnopharmacology, 2013Co-Authors: J P Chaturvedi, Nandhine Rajasekar, Ismaila O Ishola, Rakesh Shukla, Olufunmilayo O. Adeyemi, Thakur NarenderAbstract:Abstract Ethnopharmacological relevance Cnestisferruginea (CF) Vahl ex DC (Connaraceae) is a shrub widely used in traditional African medicine for the treatment of various psychiatric illness and inflammatory conditions. Aim of the study This study was carried out to investigate the effect of amentoflavone isolated from methanolic root extract of CF on lipopolysaccharide (LPS)-induced neuroinflammatory cascade of events associated to the oxidative and nitrative stress, and TNF-α production in rat Astrocytoma Cell line (C6) and human monocytic leukemia Cell line (THP-1), respectively. Materials and methods Rat Astrocytoma Cells (C6) were stimulated with LPS (10 μg/ml) alone and in the presence of different concentrations of amentoflavone (0.1–3 μg/ml) for 24 h incubation period. Nitrite release, reactive oxygen species (ROS), malondialdehyde (MDA) and reduced-glutathione (GSH) in C6 Cells were estimated; while the TNF-α level was estimated in THP-1 Cell lysate. In vivo analgesic activity was evaluated using mouse writhing and hot plate tests while the anti-inflammatory effect was investigated using carrageenan-induced oedema test. Results LPS (10 μg/ml) significantly (P Discussion and conclusion Findings in this study demonstrate the anti-neuroinflammatory and antinoceptive effects of amentoflavone which may suggest its beneficial roles in neuroinflammation associated disorders.
-
evaluation of guggulipid and nimesulide on production of inflammatory mediators and gfap expression in lps stimulated rat Astrocytoma Cell line c6
Journal of Ethnopharmacology, 2010Co-Authors: Rituraj Niranjan, Pradeep Kumar Kamat, Chandishwar Nath, Rakesh ShuklaAbstract:Abstract Aim of the study The present study was designed to investigate effect of guggulipid, a drug developed by CDRI and nimesulide on LPS stimulated neuroinflammatory changes in rat Astrocytoma Cell line (C6). Materials and methods Rat Astrocytoma Cells (C6) were stimulated with LPS (10 μg/ml) alone and in combinations with different concentrations of guggulipid or nimesulide for 24 h of incubation. Nitrite release in culture supernatant, ROS in Cells, expressions of COX-2, GFAP and TNF-α in Cell lysate were estimated. Results LPS (10 μg/ml) stimulated C6 Cells to release nitrite, ROS generation, up regulated COX-2 and GFAP expressions at protein level and TNF-α at mRNA level. Both guggulipid and nimesulide significantly attenuated nitrite release, ROS generation and also down regulated expressions of COX-2, GFAP and TNF-α. Guggulipid and nimesulide per se did not have any significant effect on C6 Cells. Conclusion Results demonstrate the anti-inflammatory effect of guggulipid comparable to nimesulide which suggest potential use of guggulipid in neuroinflammation associated conditions in CNS disorders.
Ismaila O Ishola - One of the best experts on this subject based on the ideXlab platform.
-
evaluation of amentoflavone isolated from cnestis ferruginea vahl ex dc connaraceae on production of inflammatory mediators in lps stimulated rat Astrocytoma Cell line c6 and thp 1 Cells
Journal of Ethnopharmacology, 2013Co-Authors: J P Chaturvedi, Nandhine Rajasekar, Ismaila O Ishola, Rakesh Shukla, Olufunmilayo O. Adeyemi, Thakur NarenderAbstract:Abstract Ethnopharmacological relevance Cnestisferruginea (CF) Vahl ex DC (Connaraceae) is a shrub widely used in traditional African medicine for the treatment of various psychiatric illness and inflammatory conditions. Aim of the study This study was carried out to investigate the effect of amentoflavone isolated from methanolic root extract of CF on lipopolysaccharide (LPS)-induced neuroinflammatory cascade of events associated to the oxidative and nitrative stress, and TNF-α production in rat Astrocytoma Cell line (C6) and human monocytic leukemia Cell line (THP-1), respectively. Materials and methods Rat Astrocytoma Cells (C6) were stimulated with LPS (10 μg/ml) alone and in the presence of different concentrations of amentoflavone (0.1–3 μg/ml) for 24 h incubation period. Nitrite release, reactive oxygen species (ROS), malondialdehyde (MDA) and reduced-glutathione (GSH) in C6 Cells were estimated; while the TNF-α level was estimated in THP-1 Cell lysate. In vivo analgesic activity was evaluated using mouse writhing and hot plate tests while the anti-inflammatory effect was investigated using carrageenan-induced oedema test. Results LPS (10 μg/ml) significantly (P Discussion and conclusion Findings in this study demonstrate the anti-neuroinflammatory and antinoceptive effects of amentoflavone which may suggest its beneficial roles in neuroinflammation associated disorders.
-
evaluation of amentoflavone isolated from cnestis ferruginea vahl ex dc connaraceae on production of inflammatory mediators in lps stimulated rat Astrocytoma Cell line c6 and thp 1 Cells
Journal of Ethnopharmacology, 2013Co-Authors: Ismaila O Ishola, J P Chaturvedi, Nandhine Rajasekar, Rakesh Shukla, Olufunmilayo O. Adeyemi, Shivika Rai, Thakur NarenderAbstract:Ethnopharmacological relevance Cnestisferruginea (CF) Vahl ex DC (Connaraceae) is a shrub widely used in traditional African medicine for the treatment of various psychiatric illness and inflammatory conditions. Aim of the study This study was carried out to investigate the effect of amentoflavone isolated from methanolic root extract of CF on lipopolysaccharide (LPS)-induced neuroinflammatory cascade of events associated to the oxidative and nitrative stress, and TNF-α production in rat Astrocytoma Cell line (C6) and human monocytic leukemia Cell line (THP-1), respectively. Materials and methods Rat Astrocytoma Cells (C6) were stimulated with LPS (10 μg/ml) alone and in the presence of different concentrations of amentoflavone (0.1–3 μg/ml) for 24 h incubation period. Nitrite release, reactive oxygen species (ROS), malondialdehyde (MDA) and reduced-glutathione (GSH) in C6 Cells were estimated; while the TNF-α level was estimated in THP-1 Cell lysate. In vivo analgesic activity was evaluated using mouse writhing and hot plate tests while the anti-inflammatory effect was investigated using carrageenan-induced oedema test. Results LPS (10 μg/ml) significantly (P<0.05) stimulated C6 Cells to release nitrite, ROS, MDA, and TNF-α generation while GSH was down regulated in comparison to control. However, amentoflavone significantly (P<0.05) attenuated nitrite, ROS, MDA and TNF-α generation and also up regulated the level of GSH. Amentoflavone per se did not have any significant effect on C6 and THP-1 Cells. Amentoflavone (6.25–50 mg/kg) significantly (P<0.05) reduced number of writhes and also increase pain threshold in hot plate test. It produced time course significant (P<0.05) decrease in oedema formation in rodents. Discussion and conclusion Findings in this study demonstrate the anti-neuroinflammatory and antinoceptive effects of amentoflavone which may suggest its beneficial roles in neuroinflammation associated disorders.
Thakur Narender - One of the best experts on this subject based on the ideXlab platform.
-
evaluation of amentoflavone isolated from cnestis ferruginea vahl ex dc connaraceae on production of inflammatory mediators in lps stimulated rat Astrocytoma Cell line c6 and thp 1 Cells
Journal of Ethnopharmacology, 2013Co-Authors: J P Chaturvedi, Nandhine Rajasekar, Ismaila O Ishola, Rakesh Shukla, Olufunmilayo O. Adeyemi, Thakur NarenderAbstract:Abstract Ethnopharmacological relevance Cnestisferruginea (CF) Vahl ex DC (Connaraceae) is a shrub widely used in traditional African medicine for the treatment of various psychiatric illness and inflammatory conditions. Aim of the study This study was carried out to investigate the effect of amentoflavone isolated from methanolic root extract of CF on lipopolysaccharide (LPS)-induced neuroinflammatory cascade of events associated to the oxidative and nitrative stress, and TNF-α production in rat Astrocytoma Cell line (C6) and human monocytic leukemia Cell line (THP-1), respectively. Materials and methods Rat Astrocytoma Cells (C6) were stimulated with LPS (10 μg/ml) alone and in the presence of different concentrations of amentoflavone (0.1–3 μg/ml) for 24 h incubation period. Nitrite release, reactive oxygen species (ROS), malondialdehyde (MDA) and reduced-glutathione (GSH) in C6 Cells were estimated; while the TNF-α level was estimated in THP-1 Cell lysate. In vivo analgesic activity was evaluated using mouse writhing and hot plate tests while the anti-inflammatory effect was investigated using carrageenan-induced oedema test. Results LPS (10 μg/ml) significantly (P Discussion and conclusion Findings in this study demonstrate the anti-neuroinflammatory and antinoceptive effects of amentoflavone which may suggest its beneficial roles in neuroinflammation associated disorders.
-
evaluation of amentoflavone isolated from cnestis ferruginea vahl ex dc connaraceae on production of inflammatory mediators in lps stimulated rat Astrocytoma Cell line c6 and thp 1 Cells
Journal of Ethnopharmacology, 2013Co-Authors: Ismaila O Ishola, J P Chaturvedi, Nandhine Rajasekar, Rakesh Shukla, Olufunmilayo O. Adeyemi, Shivika Rai, Thakur NarenderAbstract:Ethnopharmacological relevance Cnestisferruginea (CF) Vahl ex DC (Connaraceae) is a shrub widely used in traditional African medicine for the treatment of various psychiatric illness and inflammatory conditions. Aim of the study This study was carried out to investigate the effect of amentoflavone isolated from methanolic root extract of CF on lipopolysaccharide (LPS)-induced neuroinflammatory cascade of events associated to the oxidative and nitrative stress, and TNF-α production in rat Astrocytoma Cell line (C6) and human monocytic leukemia Cell line (THP-1), respectively. Materials and methods Rat Astrocytoma Cells (C6) were stimulated with LPS (10 μg/ml) alone and in the presence of different concentrations of amentoflavone (0.1–3 μg/ml) for 24 h incubation period. Nitrite release, reactive oxygen species (ROS), malondialdehyde (MDA) and reduced-glutathione (GSH) in C6 Cells were estimated; while the TNF-α level was estimated in THP-1 Cell lysate. In vivo analgesic activity was evaluated using mouse writhing and hot plate tests while the anti-inflammatory effect was investigated using carrageenan-induced oedema test. Results LPS (10 μg/ml) significantly (P<0.05) stimulated C6 Cells to release nitrite, ROS, MDA, and TNF-α generation while GSH was down regulated in comparison to control. However, amentoflavone significantly (P<0.05) attenuated nitrite, ROS, MDA and TNF-α generation and also up regulated the level of GSH. Amentoflavone per se did not have any significant effect on C6 and THP-1 Cells. Amentoflavone (6.25–50 mg/kg) significantly (P<0.05) reduced number of writhes and also increase pain threshold in hot plate test. It produced time course significant (P<0.05) decrease in oedema formation in rodents. Discussion and conclusion Findings in this study demonstrate the anti-neuroinflammatory and antinoceptive effects of amentoflavone which may suggest its beneficial roles in neuroinflammation associated disorders.
Mariana G Bexiga - One of the best experts on this subject based on the ideXlab platform.
-
cationic nanoparticles induce caspase 3 7 and 9 mediated cytotoxicity in a human Astrocytoma Cell line
Nanotoxicology, 2011Co-Authors: Mariana G Bexiga, Juan A Varela, Federico Fenaroli, Fengjuan Wang, Iseult Lynch, Anna Salvati, Jeremy C. Simpson, Kenneth A DawsonAbstract:AbstractOn a daily basis we are exposed to cationic nanoparticulates in many different ways. They are known to distribute to many organs of the body, and while some evidence suggests that these nanoparticles are toxic to Cells, the mechanism of their toxicity is not clear. Here we apply a combination of biochemical and imaging techniques to study the mechanism by which amine-modified polystyrene nanoparticles induce Cell death in a human brain Astrocytoma Cell line. Flow cytometry analysis of Cells exposed to cationic nanoparticles revealed an increase in Cell membrane permeability of the dyes YoPro-1 and propidium iodide, indicating onset of an apoptotic followed by a secondary necrotic response. Activation of caspases 3/7 and 9 and cleavage of poly(ADP-ribose) polymerase (PARP)-1 was also detected, providing clear molecular evidence of the apoptotic pathway induced by the nanoparticles. Transmission electron microscopy also revealed that these nanoparticles induce morphological changes in lysosomes and ...
-
cationic nanoparticles induce caspase 3 7 and 9 mediated cytotoxicity in a human Astrocytoma Cell line
Nanotoxicology, 2011Co-Authors: Mariana G Bexiga, Juan A Varela, Federico Fenaroli, Fengjuan Wang, Iseult Lynch, Anna Salvati, Jeremy C. Simpson, Kenneth A DawsonAbstract:On a daily basis we are exposed to cationic nanoparticulates in many different ways. They are known to distribute to many organs of the body, and while some evidence suggests that these nanoparticles are toxic to Cells, the mechanism of their toxicity is not clear. Here we apply a combination of biochemical and imaging techniques to study the mechanism by which amine-modified polystyrene nanoparticles induce Cell death in a human brain Astrocytoma Cell line. Flow cytometry analysis of Cells exposed to cationic nanoparticles revealed an increase in Cell membrane permeability of the dyes YoPro-1 and propidium iodide, indicating onset of an apoptotic followed by a secondary necrotic response. Activation of caspases 3/7 and 9 and cleavage of poly(ADP-ribose) polymerase (PARP)-1 was also detected, providing clear molecular evidence of the apoptotic pathway induced by the nanoparticles. Transmission electron microscopy also revealed that these nanoparticles induce morphological changes in lysosomes and mitochondria, consistent with our observation of a rapid increase in the formation of reactive oxygen species in these Cells. Together these results suggest that amine-modified polystyrene nanoparticles can mediate Cell death through an apoptotic mechanism mediated by damage to the mitochondria.
