Attached Proton Test

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François Xavier Etoa - One of the best experts on this subject based on the ideXlab platform.

  • Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract
    BMC Complementary and Alternative Medicine, 2019
    Co-Authors: Rebeca Madeleine Ebelle Etame, Raymond Simplice Mouokeu, Frank Stève Monthe Poundeu, Igor Kenfack Voukeng, Cedric Laurel Pouaha Cidjeu, Alembert Tchinda Tiabou, Abel Joel Gbaweng Yaya, Rosalie Anne Ngono Ngane, Jules Roger Kuiate, François Xavier Etoa
    Abstract:

    Background Enantia chlorantha is a plant belonging to Annonaceae Family. The Barks and leaves are used traditionally to treat infectious diseases. Earlier studies highlighted the antibacterial activity of stem barks methanol extract. This study is thus aimed at investigating the effect of fractionation on antibacterial activity of its n-butanol fraction. Methods The extract of E. chlorantha stem barks was obtained by maceration in methanol and then subjected to a liquid/liquid partition by successive depletion with solvents of increasing polarity. The n-butanol fraction was fractionated by adsorption chromatography on silica gel. A product was isolated from the dichloromethane/methanol (2%) fraction and the structure was determined on the basis of spectroscopic data; Proton Nuclear Magnetic Resonance (^1H NMR), Carbon -13 Nuclear Magnetic Resonance (^13C NMR), Heteronuclear Multiple Bond Correlation (HMBC), H-correlation spectroscopy (H-COSY), Attached Proton Test (APT), heteronuclear multiple quantum coherence (HSQC). The antibacterial activity was evaluated by broth microdilution method against six reference strains and eight clinical bacterial strains. Results The n-butanol fraction was found to be active with MIC values ranging from 32 to 256 μg/mL. The F_A sub-fraction was more efficient among the eight sub-fractions, the n-butanol fraction and comparable to Chloramphenicol used as reference antibiotic. The product obtained was elucidated as palmitin. The antibacterial activity of the latter was comparable to that of Chloramphenicol on one reference strain and 4 of the 6 clinical strains. Conclusion The F_A sub-fraction had better antibacterial activity than the n-butanol fraction and other sub-fractions, and possibly palmitin was the active substance responsible for the antibacterial activity of E. chlorantha.

  • Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract
    BMC Complementary and Alternative Medicine, 2019
    Co-Authors: Rebeca Madeleine Ebelle Etame, Raymond Simplice Mouokeu, Frank Stève Monthe Poundeu, Igor Kenfack Voukeng, Cedric Laurel Pouaha Cidjeu, Alembert Tchinda Tiabou, Abel Joel Gbaweng Yaya, Rosalie Anne Ngono Ngane, Jules Roger Kuiate, François Xavier Etoa
    Abstract:

    Enantia chlorantha is a plant belonging to Annonaceae Family. The Barks and leaves are used traditionally to treat infectious diseases. Earlier studies highlighted the antibacterial activity of stem barks methanol extract. This study is thus aimed at investigating the effect of fractionation on antibacterial activity of its n-butanol fraction. The extract of E. chlorantha stem barks was obtained by maceration in methanol and then subjected to a liquid/liquid partition by successive depletion with solvents of increasing polarity. The n-butanol fraction was fractionated by adsorption chromatography on silica gel. A product was isolated from the dichloromethane/methanol (2%) fraction and the structure was determined on the basis of spectroscopic data; Proton Nuclear Magnetic Resonance (1H NMR), Carbon-13 Nuclear Magnetic Resonance (13C NMR), Heteronuclear Multiple Bond Correlation (HMBC), H-correlation spectroscopy (H-COSY), Attached Proton Test (APT), heteronuclear multiple quantum coherence (HSQC). The antibacterial activity was evaluated by broth microdilution method against six reference strains and eight clinical bacterial strains. The n-butanol fraction was found to be active with MIC values ranging from 32 to 256 μg/mL. The FA sub-fraction was more efficient among the eight sub-fractions, the n-butanol fraction and comparable to Chloramphenicol used as reference antibiotic. The product obtained was elucidated as palmitin. The antibacterial activity of the latter was comparable to that of Chloramphenicol on one reference strain and 4 of the 6 clinical strains. The FA sub-fraction had better antibacterial activity than the n-butanol fraction and other sub-fractions, and possibly palmitin was the active substance responsible for the antibacterial activity of E. chlorantha.

Stephen B Kahl - One of the best experts on this subject based on the ideXlab platform.

Matej Krajnc - One of the best experts on this subject based on the ideXlab platform.

  • Kinetic modeling of the peroxide cross-linking of polymers: From a theoretical model framework to its application for a complex polymer system
    Chemical Engineering and Processing, 2011
    Co-Authors: Blaž Likozar, Matej Krajnc
    Abstract:

    Peroxides react with polymers in a variety of ways. The fundamental comprehension and prediction of pertinent kinetics of reactions is consequently indispensable. Based on the mechanisms of reactions of cross-linking process, a new theoretical kinetic model framework was developed. The kinetic model was then applied to the reactions of cross-linking process of various peroxides and a chosen complex polymer compound, namely partially hydrogenated poly(acrylonitrile-co-1,3-butadiene). Whereas the initial macromolecular backbone structure was determined utilizing Attached Proton Test carbon nuclear magnetic resonance, the evolution of overall concentration of cross-links was monitored through viscoelastic characteristics of the system. The model demonstrated good agreement with experimentally measured data and, moreover; the evolution of concentrations of various crucial species inherent to the cross-linking process were predicted. The most significant advantage of the developed kinetic model is that it may be readily applied to an assortment of polymer/peroxide systems.

  • Kinetic modeling of the peroxide cross-linking of polymers: From a theoretical model framework to its application for a complex polymer system
    Chemical Engineering and Processing: Process Intensification, 2011
    Co-Authors: Blaž Likozar, Matej Krajnc
    Abstract:

    Peroxides react with polymers in a variety of ways. The fundamental comprehension and prediction of pertinent kinetics of reactions is consequently indispensable. Based on the mechanisms of reactions of cross-linking process, a new theoretical kinetic model framework was developed. The kinetic model was then applied to the reactions of cross-linking process of various peroxides and a chosen complex polymer compound, namely partially hydrogenated poly(acrylonitrile-co-1,3-butadiene). Whereas the initial macromolecular backbone structure was determined utilizing Attached Proton Test carbon nuclear magnetic resonance, the evolution of overall concentration of cross-links was monitored through viscoelastic characteristics of the system. The model demonstrated good agreement with experimentally measured data and, moreover; the evolution of concentrations of various crucial species inherent to the cross-linking process were predicted. The most significant advantage of the developed kinetic model is that it may be readily applied to an assortment of polymer/peroxide systems. © 2011 Elsevier B.V.

Lemi Turker - One of the best experts on this subject based on the ideXlab platform.

  • The synthesis and investigation of the antimicrobial activity of some new phenylselanyl-1-(toluene-4-sulfonyl)-1H-tetrazole derivatives
    Medicinal Chemistry Research, 2009
    Co-Authors: Yılmaz Yıldırır, Ali Disli, M. Faruk Us, Naki Çolak, Serkan Yavuz, Hamdi Özkan, Sahlan Ozturk, Lemi Turker
    Abstract:

    The synthesis and investigation of the antimicrobial activity of some new phenylselanyl-1-(toluene-4-sulfonyl)-1H-tetrazole derivatives Yılmaz Yıldırır, M. Faruk Us, Naki Çolak, Hamdi Özkan, Serkan Yavuz, Ali Disli, Sahlan Ozturk, Lemi Turker In this study, some new phenylselanyl–1–(toluene-4-sulfonyl)-1H- tetrazole derivatives, 2a–f, have been synthesized using DCC/DMAP. In addition to, antimicrobial activity of synthesized compounds and two antibiotics (SMX, SRZ) were investigated against some microorganisms. Reagents and conditions: (i) HCI, (ii) EtONO, -5 – 0°C; (iii) KSeCN, (iv) NaN_3, Et_3NH^+ CI^−, Toluene. 2a . R_1:H, R_2:Cl 2b . 2a . R_1:H, R_2:CH_3 2c . R_1:Cl, R_2:H 2d . R_1:Br, R_2:H 2e . R_1:I, R_2:H 2f . R_1:CH_3, R_2:H The sulfonamide functional group has aroused interest in both medicinal and bioorganic chemistry. In this study, some new phenylselanyl–1–(toluene-4-sulfonyl)-1H-tetrazole derivatives (2a–f) have been synthesized using dicyclohexylcarbodiimide/dimethylaminopyridine (DCC/DMAP). The structures of the presently synthesized compounds were elucidated by spectroscopic methods [Fourier-transform infrared (FTIR) spectroscopy, ^1H nuclear magnetic resonance (NMR), ^13C nuclear magnetic resonance-Attached Proton Test (^13C NMR-APT), and mass spectrometry (MS)]. In addition, the antimicrobial activity of the synthesized compounds and two antibiotics [sulfamethoxazole (SMX) and sulfamerazine (SRZ)] were investigated against some microorganisms.

  • The synthesis and investigation of the antimicrobial activity of some new phenylselanyl-1-(toluene-4- sulfonyl)-1H-tetrazole derivatives
    Medicinal Chemistry Research, 2008
    Co-Authors: Yılmaz Yıldırır, Ali Disli, M. Faruk Us, Naki Çolak, Serkan Yavuz, Hamdi Özkan, Sahlan Ozturk, Lemi Turker
    Abstract:

    The sulfonamide functional group has aroused interest in both medicinal and bioorganic chemistry. In this study, some new phenylselanyl–1–(toluene-4-sulfonyl)-1H-tetrazole derivatives (2a–f) have been synthesized using dicyclohexylcarbodiimide/dimethylaminopyridine (DCC/DMAP). The structures of the presently synthesized compounds were elucidated by spectroscopic methods [Fourier-transform infrared (FTIR) spectroscopy, 1H nuclear magnetic resonance (NMR), 13C nuclear magnetic resonance-Attached Proton Test (13C NMR-APT), and mass spectrometry (MS)]. In addition, the antimicrobial activity of the synthesized compounds and two antibiotics [sulfamethoxazole (SMX) and sulfamerazine (SRZ)] were investigated against some microorganisms.

Rebeca Madeleine Ebelle Etame - One of the best experts on this subject based on the ideXlab platform.

  • Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract
    BMC Complementary and Alternative Medicine, 2019
    Co-Authors: Rebeca Madeleine Ebelle Etame, Raymond Simplice Mouokeu, Frank Stève Monthe Poundeu, Igor Kenfack Voukeng, Cedric Laurel Pouaha Cidjeu, Alembert Tchinda Tiabou, Abel Joel Gbaweng Yaya, Rosalie Anne Ngono Ngane, Jules Roger Kuiate, François Xavier Etoa
    Abstract:

    Background Enantia chlorantha is a plant belonging to Annonaceae Family. The Barks and leaves are used traditionally to treat infectious diseases. Earlier studies highlighted the antibacterial activity of stem barks methanol extract. This study is thus aimed at investigating the effect of fractionation on antibacterial activity of its n-butanol fraction. Methods The extract of E. chlorantha stem barks was obtained by maceration in methanol and then subjected to a liquid/liquid partition by successive depletion with solvents of increasing polarity. The n-butanol fraction was fractionated by adsorption chromatography on silica gel. A product was isolated from the dichloromethane/methanol (2%) fraction and the structure was determined on the basis of spectroscopic data; Proton Nuclear Magnetic Resonance (^1H NMR), Carbon -13 Nuclear Magnetic Resonance (^13C NMR), Heteronuclear Multiple Bond Correlation (HMBC), H-correlation spectroscopy (H-COSY), Attached Proton Test (APT), heteronuclear multiple quantum coherence (HSQC). The antibacterial activity was evaluated by broth microdilution method against six reference strains and eight clinical bacterial strains. Results The n-butanol fraction was found to be active with MIC values ranging from 32 to 256 μg/mL. The F_A sub-fraction was more efficient among the eight sub-fractions, the n-butanol fraction and comparable to Chloramphenicol used as reference antibiotic. The product obtained was elucidated as palmitin. The antibacterial activity of the latter was comparable to that of Chloramphenicol on one reference strain and 4 of the 6 clinical strains. Conclusion The F_A sub-fraction had better antibacterial activity than the n-butanol fraction and other sub-fractions, and possibly palmitin was the active substance responsible for the antibacterial activity of E. chlorantha.

  • Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract
    BMC Complementary and Alternative Medicine, 2019
    Co-Authors: Rebeca Madeleine Ebelle Etame, Raymond Simplice Mouokeu, Frank Stève Monthe Poundeu, Igor Kenfack Voukeng, Cedric Laurel Pouaha Cidjeu, Alembert Tchinda Tiabou, Abel Joel Gbaweng Yaya, Rosalie Anne Ngono Ngane, Jules Roger Kuiate, François Xavier Etoa
    Abstract:

    Enantia chlorantha is a plant belonging to Annonaceae Family. The Barks and leaves are used traditionally to treat infectious diseases. Earlier studies highlighted the antibacterial activity of stem barks methanol extract. This study is thus aimed at investigating the effect of fractionation on antibacterial activity of its n-butanol fraction. The extract of E. chlorantha stem barks was obtained by maceration in methanol and then subjected to a liquid/liquid partition by successive depletion with solvents of increasing polarity. The n-butanol fraction was fractionated by adsorption chromatography on silica gel. A product was isolated from the dichloromethane/methanol (2%) fraction and the structure was determined on the basis of spectroscopic data; Proton Nuclear Magnetic Resonance (1H NMR), Carbon-13 Nuclear Magnetic Resonance (13C NMR), Heteronuclear Multiple Bond Correlation (HMBC), H-correlation spectroscopy (H-COSY), Attached Proton Test (APT), heteronuclear multiple quantum coherence (HSQC). The antibacterial activity was evaluated by broth microdilution method against six reference strains and eight clinical bacterial strains. The n-butanol fraction was found to be active with MIC values ranging from 32 to 256 μg/mL. The FA sub-fraction was more efficient among the eight sub-fractions, the n-butanol fraction and comparable to Chloramphenicol used as reference antibiotic. The product obtained was elucidated as palmitin. The antibacterial activity of the latter was comparable to that of Chloramphenicol on one reference strain and 4 of the 6 clinical strains. The FA sub-fraction had better antibacterial activity than the n-butanol fraction and other sub-fractions, and possibly palmitin was the active substance responsible for the antibacterial activity of E. chlorantha.