The Experts below are selected from a list of 1668 Experts worldwide ranked by ideXlab platform
Ann Robinson - One of the best experts on this subject based on the ideXlab platform.
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detection of acid fast bacilli in concentrated primary specimen smears stained with rhodamine Auramine at room temperature and at 37 degrees c
Journal of Clinical Microbiology, 1994Co-Authors: Yvette S Mccarter, Ann RobinsonAbstract:Many laboratory workers prefer the rhodamine-Auramine method of staining acid-fast bacilli (AFB) in primary specimen smears rather than carbol fuchsin stains because the stain is more readily interpreted and yields greater sensitivity. The increasing incidence of AFB infections serves as an impetus to optimize the rhodamine-Auramine stain. A total of 782 primary smears were evaluated blindly by the rhodamine-Auramine method at both room temperature and 37 degrees C. Thirty-five smears (4.5%) were positive for AFB, 30 were positive by both methods, and 5 were positive at 37 degrees C only. Room temperature staining detected only 85.7% of the positive primary smears. Of the 30 smears positive by both methods, 13 (43.3%) had equal numbers of AFB on both smears, 13 (43.3%) had more AFB on the smear stained at 37 degrees C, and 4 (13.3%) had greater numbers of AFB on the smear stained at room temperature. No smears were positive only when stained at room temperature. The increasing diagnostic emphasis placed on the primary smear underscores the importance of optimizing AFB smear methods, and rhodamine-Auramine staining at 37 degrees C enhances the detection of AFB compared with conventional staining at room temperature.
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Detection of Acid-Fast Bacilli in Concentrated Primary Specimen Smears Stained with Rhodamine-Auramine at Room Temperature and at 37°C
1994Co-Authors: Yvette S Mccarter, Ann RobinsonAbstract:Many laboratory workers prefer the rhodamine-Auramine method of staining acid-fast bacilli (AFB) in primary specimen smears rather than carbol fuchsin stains because the stain is more readily interpreted and yields greater sensitivity. The increasing incidence of AFB infections serves as an impetus to optimize the rhodamine-Auramine stain. A total of 782 primary smears were evaluated blindly by the rhodamine-Auramine method at both room temperature and 3rC. Thirty-five smears (4.5%) were positive for AFB, 30 were positive by both methods, and 5 were positive at 37°C only. Room temperature staining detected only 85.7 % of the positive primary smears. Of the 30 smears positive by both methods, 13 (43.3%) had equal numbers ofAFB on both smears, 13 (43.3%) had more AFB on the smear stained at 37°C, and 4 (13.3%) had greater numbers of AFB on the smear stained at room temperature. No smears were positive only when stained at room temperature. The increasing diagnostic emphasis placed on the primary smear underscores the importance of optimizing AFB smear methods, and rhodamine-Auramine staining at 37°C enhances the detection of AFB compared with conventional staining at room temperature. Between 1953 and 1984, the number of reported cases of tuberculosis in the United States declined. Since then, the upward trend in the incidence of tuberculosis has continue
Yvette S Mccarter - One of the best experts on this subject based on the ideXlab platform.
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detection of acid fast bacilli in concentrated primary specimen smears stained with rhodamine Auramine at room temperature and at 37 degrees c
Journal of Clinical Microbiology, 1994Co-Authors: Yvette S Mccarter, Ann RobinsonAbstract:Many laboratory workers prefer the rhodamine-Auramine method of staining acid-fast bacilli (AFB) in primary specimen smears rather than carbol fuchsin stains because the stain is more readily interpreted and yields greater sensitivity. The increasing incidence of AFB infections serves as an impetus to optimize the rhodamine-Auramine stain. A total of 782 primary smears were evaluated blindly by the rhodamine-Auramine method at both room temperature and 37 degrees C. Thirty-five smears (4.5%) were positive for AFB, 30 were positive by both methods, and 5 were positive at 37 degrees C only. Room temperature staining detected only 85.7% of the positive primary smears. Of the 30 smears positive by both methods, 13 (43.3%) had equal numbers of AFB on both smears, 13 (43.3%) had more AFB on the smear stained at 37 degrees C, and 4 (13.3%) had greater numbers of AFB on the smear stained at room temperature. No smears were positive only when stained at room temperature. The increasing diagnostic emphasis placed on the primary smear underscores the importance of optimizing AFB smear methods, and rhodamine-Auramine staining at 37 degrees C enhances the detection of AFB compared with conventional staining at room temperature.
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Detection of Acid-Fast Bacilli in Concentrated Primary Specimen Smears Stained with Rhodamine-Auramine at Room Temperature and at 37°C
1994Co-Authors: Yvette S Mccarter, Ann RobinsonAbstract:Many laboratory workers prefer the rhodamine-Auramine method of staining acid-fast bacilli (AFB) in primary specimen smears rather than carbol fuchsin stains because the stain is more readily interpreted and yields greater sensitivity. The increasing incidence of AFB infections serves as an impetus to optimize the rhodamine-Auramine stain. A total of 782 primary smears were evaluated blindly by the rhodamine-Auramine method at both room temperature and 3rC. Thirty-five smears (4.5%) were positive for AFB, 30 were positive by both methods, and 5 were positive at 37°C only. Room temperature staining detected only 85.7 % of the positive primary smears. Of the 30 smears positive by both methods, 13 (43.3%) had equal numbers ofAFB on both smears, 13 (43.3%) had more AFB on the smear stained at 37°C, and 4 (13.3%) had greater numbers of AFB on the smear stained at room temperature. No smears were positive only when stained at room temperature. The increasing diagnostic emphasis placed on the primary smear underscores the importance of optimizing AFB smear methods, and rhodamine-Auramine staining at 37°C enhances the detection of AFB compared with conventional staining at room temperature. Between 1953 and 1984, the number of reported cases of tuberculosis in the United States declined. Since then, the upward trend in the incidence of tuberculosis has continue
Lingxin Chen - One of the best experts on this subject based on the ideXlab platform.
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preparation of stoichiometric molecularly imprinted polymer coatings on magnetic particles for the selective extraction of Auramine o from water
Journal of Separation Science, 2018Co-Authors: Wenwu Yang, Turghun Muhammad, Aziguli Yigaimu, Kipayem Muhammad, Lingxin ChenAbstract:A novel magnetic molecularly imprinted polymer for the selective recognition of Auramine O was rationally designed via screening from a library of nonimprinted polymers. A stoichiometric ratio of functional monomer (itaconic acid) and template molecule (Auramine O) was found to be 1.5. Meanwhile, the synthesized SiO2 @Fe3 O4 was modified by 0.5 mol/L hydrochloric acid to facilitate the preparation of magnetic molecularly imprinted polymer particles. Adsorption experiments showed that the magnetic polymer particles exhibited good selectivity, recoveries, and enrichment performance. The stoichiometric imprinted polymers have been employed for the selective preconcentration of Auramine O from lake water sample. The high specificity of the stoichiometric imprinted polymers was proven in the extraction of mixture solution of Auramine O, Auramine O hydrochloride, and chrysoidine, and the recoveries ranged between 99.66 and 108.75% (RSD 2.6-3.7%, n = 3) for lake water. These results suggest that this method is effective and can be successfully applied to the analysis of Auramine O in environmental water samples.
Nicholas S Agoff - One of the best experts on this subject based on the ideXlab platform.
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Auramine Orange Stain With Fluorescence Microscopy is a Rapid and Sensitive Technique for the Detection of Cervical Lymphadenitis Due to Mycobacterial Infection Using Fine Needle Aspiration Cytology: A Case Series
2016Co-Authors: Alan G Cheng, Anthony Chang, Gregory D Farwell, Nicholas S AgoffAbstract:OBJECTIVE: We sought to evaluate the effectiveness of the Auramine orange (AO) stain in diagnosing mycobacterial cervical adenitis (MCA) from fine needle aspiration (FNA) cytology. METHODS: A retrospective review of 19 patients evaluated at 2 urban hospitals from 2000 to 2003 for suspected MCA. FNA specimens were inoculated to culture media and had direct smears stained by the Auramine acid fast method. RESULTS: Mycobacteria were identified in 16 (84.2%) of 19 AO-stained FNA specimens, with results available within 4 hours. Corresponding cultures were positive for mycobacteria in 12 spec-imens, 9 tuberculous and 3 nontuberculous, and grew Mycobacte-rium tuberculosis from the 3 AO-negative specimens. Three of the 4 patients with negative cultures had previously taken anti-myco-bacterial medications. CONCLUSION: The AO stain with fluorescence microscopy is a sensitive and rapid method for detecting tuberculous and nontu-berculous mycobacteria. It is a valuable tool for the otolaryngolo-gists and pathologists in the diagnosis of MCA
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Auramine orange stain with fluorescence microscopy is a rapid and sensitive technique for the detection of cervical lymphadenitis due to mycobacterial infection using fine needle aspiration cytology a case series
Otolaryngology-Head and Neck Surgery, 2005Co-Authors: Alan G Cheng, Anthony Chang, Gregory D Farwell, Nicholas S AgoffAbstract:OBJECTIVE: We sought to evaluate the effectiveness of the Auramine orange (AO) stain in diagnosing mycobacterial cervical adenitis (MCA) from fine needle aspiration (FNA) cytology. METHODS: A retrospective review of 19 patients evaluated at 2 urban hospitals from 2000 to 2003 for suspected MCA. FNA specimens were inoculated to culture media and had direct smears stained by the Auramine acid fast method.RESULTS: Mycobacteria were identified in 16 (84.2%) of 19 AO-stained FNA specimens, with results available within 4 hours. Corresponding cultures were positive for mycobacteria in 12 specimens, 9 tuberculous and 3 nontuberculous, and grew Mycobacterium tuberculosis from the 3 AO-negative specimens. Three of the 4 patients with negative cultures had previously taken anti-mycobacterial medications.CONCLUSION: The AO stain with fluorescence microscopy is a sensitive and rapid method for detecting tuberculous and nontuberculous mycobacteria. It is a valuable tool for the otolaryngologists and pathologists in th...
Cassiana Carolina Montagner - One of the best experts on this subject based on the ideXlab platform.
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Auramine dyes induce toxic effects to aquatic organisms from different trophic levels an application of predicted non effect concentration pnec
Environmental Science and Pollution Research, 2021Co-Authors: Carina Cristina De Jesus Azevedo, Rhaul Oliveira, Paula Suaresrocha, Diego Sousamoura, Cesar Koppe Grisolia, Gisela De Aragao Umbuzeiro, Cassiana Carolina MontagnerAbstract:The dyes Auramine and Auramine O are used in several industrial products, despite the scarce information regarding their ecotoxicity. The aim of the present study was to assess the acute and chronic toxicity of both dyes to aquatic organisms from different trophic levels (Raphidocelis subcapitata, Daphnia similis, Hydra attenuata, and Danio rerio) and calculate their predicted non-effect concentrations (PNEC). Auramine and Auramine O induced toxicity to all selected test organisms with L(E)C50 values ranging from 300 to 4800 ug/L. Both dyes induced inhibition in the growth rate of exposed algae, negatively affecting the reproduction of D. similis and induced deformities in H. attenuata (clubbed tentacles and shortened tentacles) and D. rerio (edemas, tail malformation and delay in yolk sac absorption). PNEC values of 0.92 μg/L and 4.0 μg/L were obtained for Auramine and Auramine O, respectively, based on results of the most sensitive test system (algae). Test results were analyzed using the Criteria of Reporting and Evaluating Ecotoxicity Data (CRED), confirming their reliability and relevance. Thus, PNEC values can be used in future risk assessments of those substances in freshwater systems.
