The Experts below are selected from a list of 1611 Experts worldwide ranked by ideXlab platform
P P M Van Zuijlen - One of the best experts on this subject based on the ideXlab platform.
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a photo crosslinkable Cartilage derived extracellular matrix bioink for Auricular Cartilage tissue engineering
Acta Biomaterialia, 2021Co-Authors: Dafydd O Visscher, Anthony Atala, James J Yoo, P P M Van Zuijlen, Hyeongjin Lee, Marco N Helder, Sang Jin LeeAbstract:Three-dimensional (3D) bioprinting of patient-specific Auricular Cartilage constructs could aid in the reconstruction process of traumatically injured or congenitally deformed ear Cartilage. To achieve this, a hydrogel-based bioink is required that recapitulates the complex Cartilage microenvironment. Tissue-derived decellularized extracellular matrix (dECM)-based hydrogels have been used as bioinks for cell-based 3D bioprinting because they contain tissue-specific ECM components that play a vital role in cell adhesion, growth, and differentiation. In this study, porcine Auricular Cartilage tissues were isolated and decellularized, and the decellularized Cartilage tissues were characterized by histology, biochemical assay, and proteomics. This Cartilage-derived dECM (cdECM) was subsequently processed into a photo-crosslinkable hydrogel using methacrylation (cdECMMA) and mixed with chondrocytes to create a printable bioink. The rheological properties, printability, and in vitro biological properties of the cdECMMA bioink were examined. The results showed cdECM was obtained with complete removal of cellular components while preserving major ECM proteins. After methacrylation, the cdECMMA bioinks were printed in anatomical ear shape and exhibited adequate mechanical properties and structural integrity. Specifically, Auricular chondrocytes in the printed cdECMMA hydrogel constructs maintained their viability and proliferation capacity and eventually produced Cartilage ECM components, including collagen and glycosaminoglycans (GAGs). The potential of cell-based bioprinting using this Cartilage-specific dECMMA bioink is demonstrated as an alternative option for Auricular Cartilage reconstruction.
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design and fabrication of a hybrid alginate hydrogel poly e caprolactone mold for Auricular Cartilage reconstruction
Journal of Biomedical Materials Research Part B, 2019Co-Authors: Dafydd O Visscher, Andrew Gleadall, J K Buskermolen, Federica Burla, Joel Segal, Gijsje H Koenderink, M N Helder, P P M Van ZuijlenAbstract:The aim of this study was to design and manufacture an easily assembled Cartilage implant model for Auricular reconstruction. First, the printing accuracy and mechanical properties of 3D-printed poly-e-caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the Cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neoCartilage formation, and printing accuracy), a hybrid Auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced Cartilage-like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p > .05). The final Auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid Auricular mold appears to be an adequate model for Cartilage tissue engineering and may provide a novel approach to Auricular Cartilage regeneration for facial reconstruction. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1711-1721, 2019.
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design and fabrication of a hybrid alginate hydrogel poly e caprolactone mold for Auricular Cartilage reconstruction
J. Biomed. Mater. Res. B_Applied Biomater., 2018Co-Authors: Dafydd O Visscher, Andrew Gleadall, J K Buskermolen, Federica Burla, Joel Segal, Gijsje H Koenderink, M N Helder, P P M Van ZuijlenAbstract:The aim of this study was to design and manufacture an easily assembled Cartilage implant model for Auricular reconstruction. First, the printing accuracy and mechanical properties of 3D‐printed poly‐e‐caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the Cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neoCartilage formation, and printing accuracy), a hybrid Auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced Cartilage‐like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p > .05). The final Auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid Auricular mold appears to be an adequate model for Cartilage tissue engineering and may provide a novel approach to Auricular Cartilage regeneration for facial reconstruction.
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Design and fabrication of a hybrid alginate hydrogel/poly(ε-caprolactone) mold for Auricular Cartilage reconstruction
2018Co-Authors: Dafydd O Visscher, Andrew Gleadall, J K Buskermolen, Federica Burla, Joel Segal, Gijsje H Koenderink, M N Helder, P P M Van ZuijlenAbstract:The aim of this study was to design and manufacture an easily assembled Cartilage implant model for Auricular reconstruction. First, the printing accuracy and mechanical properties of 3D-printed poly-ε-caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material properties. Next, the applicability of alginate as cell carrier for the Cartilage implant model was determined. Using the optimal outcomes of both experiments (in terms of (bio)mechanical properties, cell survival, neoCartilage formation, and printing accuracy), a hybrid Auricular implant model was developed. PCL scaffolds with 600 μm distances between strands exhibited the best mechanical properties and most optimal printing quality for further exploration. In alginate, chondrocytes displayed high cell survival (~83% after 21 days) and produced Cartilage-like matrix in vitro. Alginate beads cultured in proliferation medium exhibited slightly higher compressive moduli (6 kPa) compared to beads cultured in chondrogenic medium (3.5 kPa, p >.05). The final Auricular mold could be printed with 300 μm pores and high fidelity, and the injected chondrocytes survived the culture period of 21 days. The presented hybrid Auricular mold appears to be an adequate model for Cartilage tissue engineering and may provide a novel approach to Auricular Cartilage regeneration for facial reconstruction
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noninvasive measurement of ear Cartilage elasticity on the cellular level a new method to provide biomechanical information for tissue engineering
Plastic and reconstructive surgery. Global open, 2017Co-Authors: Ernst Jan Bos, Marco N Helder, Koen Van Der Laan, Margriet G Mullender, D Iannuzzi, P P M Van ZuijlenAbstract:Background:An important feature of Auricular Cartilage is its stiffness. To tissue engineer new Cartilage, we need objective tools to provide us with the essential biomechanical information to mimic optimal conditions for chondrogenesis and extracellular matrix (ECM) development. In this study, we u
Alexander M Seifalian - One of the best experts on this subject based on the ideXlab platform.
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Biomechanical Characterisation of the Human Auricular Cartilages; Implications for Tissue Engineering
Annals of Biomedical Engineering, 2016Co-Authors: M. F. Griffin, Y Premakumar, M Szarko, Alexander M Seifalian, P E ButlerAbstract:Currently, autologous Cartilage provides the gold standard for Auricular reconstruction. However, synthetic biomaterials offer a number of advantages for ear reconstruction including decreased donor site morbidity and earlier surgery. Critical to implant success is the material’s mechanical properties as this affects biocompatibility and extrusion. The aim of this study was to determine the biomechanical properties of human Auricular Cartilage. Auricular Cartilage from fifteen cadavers was indented with displacement of 1 mm/s and load of 300 g to obtain a Young’s modulus in compression. Histological analysis of the auricle was conducted according to glycoprotein, collagen, and elastin content. The compression modulus was calculated for each part of the auricle with the tragus at 1.67 ± 0.61 MPa, antitragus 1.79 ± 0.56 MPa, concha 2.08 ± 0.70 MPa, antihelix 1.71 ± 0.63 MPa, and helix 1.41 ± 0.67 MPa. The concha showed to have a significantly greater Young’s Elastic Modulus than the helix in compression ( p
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a biodesigned nanocomposite biomaterial for Auricular Cartilage reconstruction
Advanced Healthcare Materials, 2016Co-Authors: Leila Nayyer, Alexander M Seifalian, Gavin Jell, Ali Esmaeili, Martin A BirchallAbstract:Current biomaterials for Auricular replacement are associated with high rates of infection and extrusion. The development of new Auricular biomaterials that mimic the mechanical properties of native tissue and promote desirable cellular interactions may prevent implant failure. A porous 3D nanocomposite scaffold (NS) based on POSS-PCU (polyhedral oligomeric silsesquioxane nanocage into polycarbonate based urea-urethane) is developed with an elastic modulus similar to native ear. In vitro biological interactions on this NS reveal greater protein adsorption, increased fibroblast adhesion, proliferation, and collagen production compared with Medpor (the current synthetic Auricular implant). In vivo, the POSS-PCU with larger pores (NS2; 150-250 μm) have greater tissue ingrowth (≈5.8× and ≈1.4 × increase) than the POSS-PCU with smaller pores (NS1; 100-50 μm) and when compared to Medpor (>100 μm). The NS2 with the larger pores demonstrates a reduced fibrotic encapsulation compared with NS1 and Medpor (≈4.1× and ≈1.6×, respectively; P < 0.05). Porosity also influences the amount of neovascularization within the implants, with no blood vessel observed in NS1 (12 weeks postimplantation). The lack of chronic inflammatory response for all materials may indicate that the elastic modulus and pore size of the implant scaffold could be important design considerations for influencing fibrotic responses to Auricular and other soft tissue implants.
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biomechanical characterisation of the human nasal Cartilages implications for tissue engineering
Journal of Materials Science: Materials in Medicine, 2016Co-Authors: Michelle Griffin, Y Premakumar, M Szarko, Alexander M Seifalian, P E ButlerAbstract:Currently, autologous Cartilage provides the gold standard for Auricular reconstruction. However, synthetic biomaterials offer a number of advantages for ear reconstruction including decreased donor site morbidity and earlier surgery. Critical to implant success is the material’s mechanical properties as this affects biocompatibility and extrusion. The aim of this study was to determine the biomechanical properties of human Auricular Cartilage. Auricular Cartilage from fifteen cadavers was indented with displacement of 1 mm/s and load of 300 g to obtain a Young’s modulus in compression. Histological analysis of the auricle was conducted according to glycoprotein, collagen, and elastin content. The compression modulus was calculated for each part of the auricle with the tragus at 1.67 ± 0.61 MPa, antitragus 1.79 ± 0.56 MPa, concha 2.08 ± 0.70 MPa, antihelix 1.71 ± 0.63 MPa, and helix 1.41 ± 0.67 MPa. The concha showed to have a significantly greater Young’s Elastic Modulus than the helix in compression (p < 0.05). The histological analysis demonstrated that the auricle has a homogenous structure in terms of chondrocyte morphology, extracellular matrix and elastin content. This study provides new information on the compressive mechanical properties and histological analysis of the human Auricular Cartilage, allowing surgeons to have a better understanding of suitable replacements. This study has provided a reference, by which Cartilage replacements should be developed for Auricular reconstruction.
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tissue engineering revolution and challenge in Auricular Cartilage reconstruction
Plastic and Reconstructive Surgery, 2012Co-Authors: Leila Nayyer, Ali Esmaeili, Martin A Birchall, Kavi H Patel, Radoslaw A Rippel, Gregory Oʼtoole, Peter E M Butler, Alexander M SeifalianAbstract:External ear reconstruction for congenital deformity such as microtia or following trauma remains one of the greatest challenges for reconstructive plastic surgeons. The problems faced in reconstructing the intricate ear framework are highly complex. A durable, inert material that is resistant to scar contracture is required. To date, no material, autologous or prosthetic, is available that perfectly mimics the shapely elastic Cartilage found in the ear. Current procedure involves autologous costal Cartilage that is sculpted to create a framework for the overlying soft tissues. However, this is associated with donor-site morbidity, and few surgeons worldwide are skilled in the techniques required to obtain excellent results. Various alloplastic materials have therefore been used as a framework. However, a degree of immunogenicity and infection and extrusion are inevitable, and results are often disappointing. Tissue-engineered Cartilage is an alternative approach but, despite significant progress in this area, many problems remain. These need to be addressed before routine clinical application will become possible. The current tissue-engineered options are fragile and inflexible. The next generation of Auricular Cartilage engineering is promising, with smart materials to enhance cell growth and integration, and the application of stem cells in a clinical setting. More recently, the authors' team designed the world's first entirely synthetic trachea composed of a novel nanocomposite material seeded with the patient's own stem cells. This was successfully transplanted in a patient at the Karolinska Hospital in Sweden and may translate into a tissue-engineered auricle in the future.
Hideki Taniguchi - One of the best experts on this subject based on the ideXlab platform.
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presence of Cartilage stem progenitor cells in adult mice Auricular perichondrium
PLOS ONE, 2011Co-Authors: Shinji Kobayashi, Takanori Takebe, Jiro Maegawa, Yunwen Zheng, Mitsuru Mizuno, Yuichiro Yabuki, Hideki TaniguchiAbstract:Background: Based on evidence from several other tissues, Cartilage stem/progenitor cells in the Auricular Cartilage presumably contribute to tissue development or homeostasis of the auricle. However, no definitive studies have identified or characterized a stem/progenitor population in mice auricle. Methodology/Principal Findings: The 5-bromo-29-deoxyuridine (BrdU) label-retaining technique was used to label dividing cells in fetal mice. Observations one year following the labeling revealed that label-retaining cells (LRCs) were present specifically in Auricular perichondrium at a rate of 0.0860.06%, but LRCs were not present in chondrium. Furthermore, LRCs were successfully isolated and cultivated from Auricular Cartilage. Immunocytochemical analyses showed that LRCs express CD44 and integrin-a5. These LRCs, putative stem/progenitor cells, possess clonogenicity and chondrogenic capability in vitro. Conclusions/Significance: We have identified a population of putative Cartilage stem/progenitor cells in the Auricular perichondrium of mice. Further characterization and utilization of the cell population should improve our understanding of basic Cartilage biology and lead to advances in Cartilage tissue engineering and novel therapeutic strategies for patients with craniofacial defects, including long-term tissue restoration.
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reconstruction of human elastic Cartilage by a cd44 cd90 stem cell in the ear perichondrium
Proceedings of the National Academy of Sciences of the United States of America, 2011Co-Authors: Shinji Kobayashi, Takanori Takebe, Jiro Maegawa, Midori Inui, Sayaka Iwai, Yunwen Zheng, Hideki TaniguchiAbstract:Despite the great demands for treating craniofacial injuries or abnormalities, effective treatments are currently lacking. One promising approach involves human elastic Cartilage reconstruction using autologous stem/progenitor populations. Nevertheless, definitive evidence of the presence of stem cells in human Auricular Cartilage remains to be established. Here, we demonstrate that human Auricular perichondrium, which can be obtained via a minimally invasive approach, harbors a unique cell population, termed as Cartilage stem/progenitor cells (CSPCs). The clonogenic progeny of a single CD44+ CD90+ CSPC displays a number of features characteristic of stem cells. Highly chondrogenic CSPCs were shown to reconstruct large (>2 cm) elastic Cartilage after extended expansion and differentiation. CSPC-derived Cartilage was encapsulated by a perichondrium layer, which contains a CD44+ CD90+ self-renewing stem/progenitor population and was maintained without calcification or tumor formation even after 10 mo. This is a unique report demonstrating the presence of stem cells in Auricular Cartilage. Utilization of CSPCs will provide a promising reconstructive material for treating craniofacial defects with successful long-term tissue restoration.
Xiaodie Zhang - One of the best experts on this subject based on the ideXlab platform.
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the in vivo chondrogenesis of Cartilage stem progenitor cells from Auricular Cartilage and the perichondrium
American Journal of Translational Research, 2019Co-Authors: Xiaodie Zhang, Zhezheng Xiong, Yahong Chen, Lin Qi, Huizhong Zhang, Peng Xu, Junjie Li, Zhuxin ChenAbstract:Bone marrow-derived stem cells are commonly studied for Cartilage tissue engineering and regeneration medicine applications, but their ossification tendency and their limited capacity for chondrogenic differentiation depending on the donor age limit their clinical application. Cartilage stem/progenitor cells are ideal seeding cells, as Cartilage stem/progenitor cells from Auricular Cartilage and the perichondrium have the inherent advantages of chondrogenesis capacity and an easy and nontraumatic harvesting process, displaying promise for applications. The identification and comparison of Cartilage stem/progenitor cells from Auricular Cartilage and the perichondrium in vitro were explored in our previous study, but the in vivo chondrogenesis of these cells has not been fully examined. In the current study, we explored the ectopic chondrogenesis of Cartilage stem progenitor/cells from Auricular Cartilage and the perichondrium after chondrogenic induction in vitro. Our results suggest that stem/progenitor cells from Auricular Cartilage exhibit significantly better chondrogenesis than those from the perichondrium in vivo, with upregulated chondrogenic genes and a stable Cartilage phenotype, as well as good mechanical properties, indicating that stem/progenitor cells from Auricular Cartilage could be one type of ideal seeding cells for Cartilage tissue engineering.
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isolation identification and comparison of Cartilage stem progenitor cells from Auricular Cartilage and perichondrium
American Journal of Translational Research, 2016Co-Authors: Xiaodie Zhang, Lin Qi, Jia ZhouAbstract:Auricular Cartilage loss or defect remains a challenge to plastic surgeons, and Cartilage regenerative medicine provides a novel method to solve the problem. However, ideal seeding cells seem to be the key point in the development of Cartilage regeneration. Although bone marrow-mesenchymal stem cells were considered as the ideal seeding cells in Cartilage regeneration, regenerative Cartilage differentiated from bone marrow-mesenchymal stem cells still faces some problems. It is reported that many tissues and organs contain a certain number of adult progenitor or stem cells that can replace cells that die or restore tissues and organs after injury. Therefore, we tried to use a fibronectin differential adhesion assay to isolate Cartilage stem/progenitor cells from Auricular Cartilage and perichondrium. Flow cytometric analysis demonstrated the two cell populations expressed mesenchyme stem cell positive surface marker. Meanwhile, the cells differentiate into osteogenic line, chondrogenic line and adipogenic line under different induction conditions. The proliferation of Cartilage stem/progenitor cells derived from perichondrium was higher than Cartilage stem/progenitor cells derived from Auricular Cartilage. In addition, there is a difference on osteogenic differentiation, chondrogenic differentiation and adipogenic differentiation between these two cell populations. In conclusion, Auricular Cartilage and perichondrium both contain Cartilage stem/progenitor cells, which may provide an ideal seeding cells for Cartilage regeneration.
Wei Zhong - One of the best experts on this subject based on the ideXlab platform.
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tissue engineering of Cartilage with the use of chitosan gelatin complex scaffolds
Journal of Biomedical Materials Research Part B, 2004Co-Authors: Wei Zhong, Juanjuan Wu, K H ChuaAbstract:Chitosan has been shown to be a promising scaffold for various applications in tissue engineering. In this study, a chitosan-gelatin complex was fabricated as a scaffold by a freezing and lyophilizing technique. Chitosan's structure and characteristics are similar to those of glycosaminoglycan (GAG) and its analogs, and possesses various biological activities, whereas gelatin can serve as a substrate for cell adhesion, differentiation, and proliferation. With the use of autologous chondrocytes isolated from pig's Auricular Cartilage and seeded onto the chitosan-gelatin scaffold, elastic Cartilages have been successfully engineered at the porcine abdomen subcutaneous tissue. After 16 weeks of implantation, the engineered elastic Cartilages have acquired not only normal histological and biochemical, but also mechanical properties. The tissue sections of the engineered elastic Cartilages showed that the chondrocytes were enclosed in the lacuna, similar to that of native Cartilage. The presence of elastic fibers in the engineered Cartilages was also demonstrated by Vehoeff's staining, and immunohistochemical staining confirmed the presence of type II collagen in the engineered Cartilages. Quantitatively, the GAG in the engineered Cartilages reached 90% of the concentration in native Auricular Cartilage. Furthermore, biomechanical analysis demonstrated that the extrinsic stiffness of the engineered Cartilages reached 85% of the level in native Auricular Cartilage when it was harvested at 16 weeks. Thus, this study demonstrated that the chitosan-gelatin complex may serve as a suitable scaffold for Cartilage tissue engineering. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 71B: 373–380, 2004
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tissue engineering of Cartilage with the use of chitosan gelatin complex scaffolds
Journal of Biomedical Materials Research Part B, 2004Co-Authors: Wanyao Xia, Wei Zhong, Kien Hui Chua, Wei Liu, Lei Cui, Yuanchun Liu, Deli Liu, Yilin CaoAbstract:Chitosan has been shown to be a promising scaffold for various applications in tissue engineering. In this study, a chitosan-gelatin complex was fabricated as a scaffold by a freezing and lyophilizing technique. Chitosan's structure and characteristics are similar to those of glycosaminoglycan (GAG) and its analogs, and possesses various biological activities, whereas gelatin can serve as a substrate for cell adhesion, differentiation, and proliferation. With the use of autologous chondrocytes isolated from pig's Auricular Cartilage and seeded onto the chitosan-gelatin scaffold, elastic Cartilages have been successfully engineered at the porcine abdomen subcutaneous tissue. After 16 weeks of implantation, the engineered elastic Cartilages have acquired not only normal histological and biochemical, but also mechanical properties. The tissue sections of the engineered elastic Cartilages showed that the chondrocytes were enclosed in the lacuna, similar to that of native Cartilage. The presence of elastic fibers in the engineered Cartilages was also demonstrated by Vehoeff's staining, and immunohistochemical staining confirmed the presence of type II collagen in the engineered Cartilages. Quantitatively, the GAG in the engineered Cartilages reached 90% of the concentration in native Auricular Cartilage. Furthermore, biomechanical analysis demonstrated that the extrinsic stiffness of the engineered Cartilages reached 85% of the level in native Auricular Cartilage when it was harvested at 16 weeks. Thus, this study demonstrated that the chitosan-gelatin complex may serve as a suitable scaffold for Cartilage tissue engineering.
