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Autologous Plasma

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Gorka Orive – One of the best experts on this subject based on the ideXlab platform.

  • release kinetics of platelet derived and Plasma derived growth factors from Autologous Plasma rich in growth factors
    Annals of Anatomy-anatomischer Anzeiger, 2013
    Co-Authors: Eduardo Anitua, Mari Mar Zalduendo, Mohammad Hamdan Alkhraisat, Gorka Orive

    Abstract:

    Summary Many studies have evaluated the biological effects of platelet rich Plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by Plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare Autologous Plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich Plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24 h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at −80 °C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1 h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich Plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich Plasma.

  • Release kinetics of platelet-derived and Plasma-derived growth factors from Autologous Plasma rich in growth factors
    Annals of Anatomy, 2013
    Co-Authors: Eduardo Anitua, Mari Mar Zalduendo, Mohammad Hamdan Alkhraisat, Gorka Orive

    Abstract:

    Many studies have evaluated the biological effects of platelet rich Plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by Plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare Autologous Plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich Plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24. h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at -80. °C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1. h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich Plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich Plasma. © 2013 Elsevier GmbH.

Igor Meglinski – One of the best experts on this subject based on the ideXlab platform.

  • RBC aggregation dynamics in Autologous Plasma and serum studied with double-channel optical tweezers
    Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 2016
    Co-Authors: Anna Danilina, Anton Potkin, Matti Kinnunen, Alexander V. Priezzhev, Igor Meglinski

    Abstract:

    Red blood cells aggregating and disaggregating forces were measured in the Autologous Plasma and serum using the double-channeled optical tweezers. A significant, three-fold decrease of the both forces was observed in the serum compared to the Plasma. The results of this study help to better assess the RBC aggregation mechanism.

  • Probing the Red Blood Cells Aggregating Force With Optical Tweezers
    IEEE Journal of Selected Topics in Quantum Electronics, 2016
    Co-Authors: Anna Danilina, Matti Kinnunen, Alexander V. Priezzhev, Igor Meglinski

    Abstract:

    The red blood cells (RBC) aggregation is of current basic science and clinical interest, as a determinant of blood microcirculation. Thus, the measurement and assessment of the RBC aggregation property (aggregability) and aggregation state at different physiologic conditions of a human individual or laboratory animal are an important issue. In this paper, in order to assess the dynamics of RBC interaction, optical tweezers were used to probe the forces during the RBC doublet formation or disruption. We show that in Autologous Plasma, RBC aggregating and disaggregating forces have different absolute values, ca 2-4 pN and dozens of piconewton, correspondingly. We speculate that in Plasma, RBC aggregation and disaggregation processes have different driving forces.

Eduardo Anitua – One of the best experts on this subject based on the ideXlab platform.

  • release kinetics of platelet derived and Plasma derived growth factors from Autologous Plasma rich in growth factors
    Annals of Anatomy-anatomischer Anzeiger, 2013
    Co-Authors: Eduardo Anitua, Mari Mar Zalduendo, Mohammad Hamdan Alkhraisat, Gorka Orive

    Abstract:

    Summary Many studies have evaluated the biological effects of platelet rich Plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by Plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare Autologous Plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich Plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24 h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at −80 °C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1 h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich Plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich Plasma.

  • Release kinetics of platelet-derived and Plasma-derived growth factors from Autologous Plasma rich in growth factors
    Annals of Anatomy, 2013
    Co-Authors: Eduardo Anitua, Mari Mar Zalduendo, Mohammad Hamdan Alkhraisat, Gorka Orive

    Abstract:

    Many studies have evaluated the biological effects of platelet rich Plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by Plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare Autologous Plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich Plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24. h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at -80. °C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1. h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich Plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich Plasma. © 2013 Elsevier GmbH.