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Gorka Orive - One of the best experts on this subject based on the ideXlab platform.
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release kinetics of platelet derived and Plasma derived growth factors from Autologous Plasma rich in growth factors
Annals of Anatomy-anatomischer Anzeiger, 2013Co-Authors: Eduardo Anitua, Mari Mar Zalduendo, Mohammad Hamdan Alkhraisat, Gorka OriveAbstract:Summary Many studies have evaluated the biological effects of platelet rich Plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by Plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare Autologous Plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich Plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24 h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at −80 °C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1 h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich Plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich Plasma.
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Release kinetics of platelet-derived and Plasma-derived growth factors from Autologous Plasma rich in growth factors
Annals of Anatomy, 2013Co-Authors: Eduardo Anitua, Mari Mar Zalduendo, Mohammad Hamdan Alkhraisat, Gorka OriveAbstract:Many studies have evaluated the biological effects of platelet rich Plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by Plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare Autologous Plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich Plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24. h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at -80. °C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1. h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich Plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich Plasma. © 2013 Elsevier GmbH.
Igor Meglinski - One of the best experts on this subject based on the ideXlab platform.
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RBC aggregation dynamics in Autologous Plasma and serum studied with double-channel optical tweezers
Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 2016Co-Authors: Anna Danilina, Anton Potkin, Matti Kinnunen, Alexander V. Priezzhev, Igor MeglinskiAbstract:Red blood cells aggregating and disaggregating forces were measured in the Autologous Plasma and serum using the double-channeled optical tweezers. A significant, three-fold decrease of the both forces was observed in the serum compared to the Plasma. The results of this study help to better assess the RBC aggregation mechanism.
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Probing the Red Blood Cells Aggregating Force With Optical Tweezers
IEEE Journal of Selected Topics in Quantum Electronics, 2016Co-Authors: Anna Danilina, Matti Kinnunen, Alexander V. Priezzhev, Igor MeglinskiAbstract:The red blood cells (RBC) aggregation is of current basic science and clinical interest, as a determinant of blood microcirculation. Thus, the measurement and assessment of the RBC aggregation property (aggregability) and aggregation state at different physiologic conditions of a human individual or laboratory animal are an important issue. In this paper, in order to assess the dynamics of RBC interaction, optical tweezers were used to probe the forces during the RBC doublet formation or disruption. We show that in Autologous Plasma, RBC aggregating and disaggregating forces have different absolute values, ca 2-4 pN and dozens of piconewton, correspondingly. We speculate that in Plasma, RBC aggregation and disaggregation processes have different driving forces.
Eduardo Anitua - One of the best experts on this subject based on the ideXlab platform.
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release kinetics of platelet derived and Plasma derived growth factors from Autologous Plasma rich in growth factors
Annals of Anatomy-anatomischer Anzeiger, 2013Co-Authors: Eduardo Anitua, Mari Mar Zalduendo, Mohammad Hamdan Alkhraisat, Gorka OriveAbstract:Summary Many studies have evaluated the biological effects of platelet rich Plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by Plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare Autologous Plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich Plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24 h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at −80 °C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1 h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich Plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich Plasma.
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Release kinetics of platelet-derived and Plasma-derived growth factors from Autologous Plasma rich in growth factors
Annals of Anatomy, 2013Co-Authors: Eduardo Anitua, Mari Mar Zalduendo, Mohammad Hamdan Alkhraisat, Gorka OriveAbstract:Many studies have evaluated the biological effects of platelet rich Plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by Plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare Autologous Plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich Plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24. h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at -80. °C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1. h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich Plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich Plasma. © 2013 Elsevier GmbH.
José Augusto Nogueira-machado - One of the best experts on this subject based on the ideXlab platform.
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Inhibition of ROS production in peripheral blood mononuclear cells from type 2 diabetic patients by Autologous Plasma depends on Akt/PKB signaling pathway.
Clinica Chimica Acta, 2008Co-Authors: Clara Araújo Veloso, Camila Armond Isoni, Erica Abreu Borges, Rafael Teixeira Mattos, M.r. Calsolari, Janice Sepúlveda Reis, Adriana A. Bosco, Míriam Martins Chaves, José Augusto Nogueira-machadoAbstract:Abstract Objective To compare the role of Akt/PKB signaling pathway in the modulation of reactive oxygen species (ROS) production by Autologous Plasma in peripheral blood mononuclear cells (PBMNC) from type 2 diabetic patients and healthy subjects. Materials and methods This study was approved by Santa Casa Ethical Committee and has included patients diagnosed with diabetes type 2 (DM2) and control group (non-diabetic) (ND). PBMNC were purified utilizing Ficoll-hypaque gradient. ROS was quantified by luminol-dependent chemiluminescence. The Akt/PKB phosphorylation was measured using a CASE kit. Statistical analyses were made with t Student test and chi-square (χ2). p Results 12, 13-Phorbol dibutyrate (PDB) stimulated the production of higher levels of ROS in PBMNC from type 2 diabetic patients than that from healthy subjects. Autologous Plasma, however, inhibited induced or not ROS production in PBMNC in both groups. The inhibition of PBMNC-ROS derived by Autologous Plasma from healthy subjects was higher than that from type 2 diabetic patients. Plasma phosphorylated (activated) Akt/PKB. The percentage of phosphorylation induced by Autologous Plasma in PBMNC from patients and healthy control were 14% and 93%, respectively. Inhibition of ROS production in PBMNC from DM2 were similar for PBMNC + Plasma; PBMNC + Akti; and PBMNC + Plasma + Akti. However, in ND control, Plasma showed a higher ROS inhibition than Akti or Plasma plus Akti. Conclusions Our results suggest that the low antioxidant capacity observed in Autologous Plasma from DM2 patients in conjunction with the decreased activation of PKB may cause an imbalance in the oxidizing/reducing responses, possible inducing an oxidative stress state, which could be associated with tissular damage.
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Inhibition of ROS production in peripheral blood mononuclear cells from type 2 diabetic patients by Autologous Plasma depends on Akt/PKB signaling pathway.
Clinica chimica acta; international journal of clinical chemistry, 2008Co-Authors: Clara Araújo Veloso, Camila Armond Isoni, Erica Abreu Borges, Rafael Teixeira Mattos, M.r. Calsolari, Janice Sepúlveda Reis, Adriana A. Bosco, Míriam Martins Chaves, José Augusto Nogueira-machadoAbstract:To compare the role of Akt/PKB signaling pathway in the modulation of reactive oxygen species (ROS) production by Autologous Plasma in peripheral blood mononuclear cells (PBMNC) from type 2 diabetic patients and healthy subjects. This study was approved by Santa Casa Ethical Committee and has included patients diagnosed with diabetes type 2 (DM2) and control group (non-diabetic) (ND). PBMNC were purified utilizing Ficoll-hypaque gradient. ROS was quantified by luminol-dependent chemiluminescence. The Akt/PKB phosphorylation was measured using a CASE kit. Statistical analyses were made with t Student test and chi-square (chi(2)). p<0.05 was considered significant. 12, 13-Phorbol dibutyrate (PDB) stimulated the production of higher levels of ROS in PBMNC from type 2 diabetic patients than that from healthy subjects. Autologous Plasma, however, inhibited induced or not ROS production in PBMNC in both groups. The inhibition of PBMNC-ROS derived by Autologous Plasma from healthy subjects was higher than that from type 2 diabetic patients. Plasma phosphorylated (activated) Akt/PKB. The percentage of phosphorylation induced by Autologous Plasma in PBMNC from patients and healthy control were 14% and 93%, respectively. Inhibition of ROS production in PBMNC from DM2 were similar for PBMNC+Plasma; PBMNC+Akti; and PBMNC+Plasma+Akti. However, in ND control, Plasma showed a higher ROS inhibition than Akti or Plasma plus Akti. Our results suggest that the low antioxidant capacity observed in Autologous Plasma from DM2 patients in conjunction with the decreased activation of PKB may cause an imbalance in the oxidizing/reducing responses, possible inducing an oxidative stress state, which could be associated with tissular damage.
Vincent Robert - One of the best experts on this subject based on the ideXlab platform.
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Malaria transmission-blocking activity in experimental infections of Anopheles gambiae from naturally infected Plasmodium falciparum gametocyte carriers.
Transactions of The Royal Society of Tropical Medicine and Hygiene, 1994Co-Authors: Bert Mulder, Timoléon Tchuinkam, Koen J. Dechering, Jan Peter Verhave, Pierre Carnevale, J.h.e.t. Meuwissen, Vincent RobertAbstract:Experimental infections of anopheline mosquitoes were carried out with Plasmodium falciparum gametocytes from 65 naturally infected patients in Cameroon. A comparison was made between infections with blood containing Autologous Plasma and blood in which the Plasma was replaced with Plasma from a donor without previous malaria exposure. A lower infection rate was observed in 50 of 65 Autologous Plasma samples. Transmission was significantly blocked in 3 infections. This indicates that, in a population living in an area endemic for malaria, blood Plasma factor(s) can reduce the transmission capacity of gametocyte carriers to mosquitoes.
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Malaria transmission-blocking activity in the Plasma of Plasmodium falciparum gametocyte carriers in Cameroon.
Parassitologia, 1993Co-Authors: Bert Mulder, Timoléon Tchuinkam, Jan Peter Verhave, Vincent RobertAbstract:Experimental infections of Anopheles gambiae were carried out with Plasmodium falciparum gametocytes from 65 naturally infected patients in Cameroon. A comparison was made between infections with blood containing Autologous Plasma and blood in which the Plasma was replaced by Plasma from donors without previous malaria exposure. A lower mosquito-infection rate was observed in 50 out of 65 Autologous Plasma samples. The transmission was completely blocked in 8 infections, whilst belonging exposures to heterologous Plasma led to infected mosquitoes. Evidence is shown that blood Plasma factors of gametocyte carriers from a population living in a malaria-endemic area are able to reduce transmission capacity.