The Experts below are selected from a list of 3819 Experts worldwide ranked by ideXlab platform
Godfred Agongo - One of the best experts on this subject based on the ideXlab platform.
-
candidate gene analysis reveals strong association of cetp variants with high density lipoprotein cholesterol and pcsk9 variants with low density lipoprotein cholesterol in ghanaian adults an awi gen sub study
Frontiers in Genetics, 2020Co-Authors: Godfred Agongo, Lucas Amengaetego, Engelbert A Nonterah, Cornelius Debpuur, Ananyo Choudhury, Amy R Bentley, Abraham Oduro, Charles N Rotimi, Nigel J CrowtherAbstract:Variations in lipid levels are attributed partly to genetic factors. Genome-wide association studies (GWASs) mainly performed in European, African American and Asian cohorts have identified variants associated with LDL-C, HDL-C, total cholesterol (TC) and triglycerides (TG), but few studies have been performed in sub-Saharan Africans. This study evaluated the effect of single nucleotide variants (SNVs) in eight candidate loci (ABCA1, LCAT, LPL, PON1, CETP, PCSK9, MVK, and MMAB) on lipid levels among 1855 Ghanaian adults. All lipid levels were measured directly using an Automated Analyser. DNA was extracted and genotyped using the H3Africa SNV array. Linear regression models were used to test the association between SNVs and log-transformed lipid levels, adjusting for sex, age and waist circumference. In addition Bonferroni correction was performed to account for multiple testing. Several variants of CETP, LCAT, PCSK9, and PON1 (MAF > 0.05) were associated with HDL-C, LDL-C and TC levels at p < 0.05. The lead variants for association with HDL-C were rs17231520 in CETP (β = 0.139, p < 0.0001) and rs1109166 in LCAT (β = -0.044, p = 0.028). Lower LDL-C levels were associated with an intronic variant in PCSK9 (rs11806638 [β = -0.055, p = 0.027]) and increased TC was associated with a variant in PON1 (rs854558 [β = 0.040, p = 0.020]). In silico functional analyses indicated that these variants likely influence gene function through their effect on gene transcription. We replicated a strong association between CETP variants and HDL-C and between PCSK9 variant and LDL-C in West Africans, with two potentially functional variants and identified three novel variants in linkage disequilibrium in PON1 which were associated with increasing TC levels in Ghanaians.
Nigel J Crowther - One of the best experts on this subject based on the ideXlab platform.
-
candidate gene analysis reveals strong association of cetp variants with high density lipoprotein cholesterol and pcsk9 variants with low density lipoprotein cholesterol in ghanaian adults an awi gen sub study
Frontiers in Genetics, 2020Co-Authors: Godfred Agongo, Lucas Amengaetego, Engelbert A Nonterah, Cornelius Debpuur, Ananyo Choudhury, Amy R Bentley, Abraham Oduro, Charles N Rotimi, Nigel J CrowtherAbstract:Variations in lipid levels are attributed partly to genetic factors. Genome-wide association studies (GWASs) mainly performed in European, African American and Asian cohorts have identified variants associated with LDL-C, HDL-C, total cholesterol (TC) and triglycerides (TG), but few studies have been performed in sub-Saharan Africans. This study evaluated the effect of single nucleotide variants (SNVs) in eight candidate loci (ABCA1, LCAT, LPL, PON1, CETP, PCSK9, MVK, and MMAB) on lipid levels among 1855 Ghanaian adults. All lipid levels were measured directly using an Automated Analyser. DNA was extracted and genotyped using the H3Africa SNV array. Linear regression models were used to test the association between SNVs and log-transformed lipid levels, adjusting for sex, age and waist circumference. In addition Bonferroni correction was performed to account for multiple testing. Several variants of CETP, LCAT, PCSK9, and PON1 (MAF > 0.05) were associated with HDL-C, LDL-C and TC levels at p < 0.05. The lead variants for association with HDL-C were rs17231520 in CETP (β = 0.139, p < 0.0001) and rs1109166 in LCAT (β = -0.044, p = 0.028). Lower LDL-C levels were associated with an intronic variant in PCSK9 (rs11806638 [β = -0.055, p = 0.027]) and increased TC was associated with a variant in PON1 (rs854558 [β = 0.040, p = 0.020]). In silico functional analyses indicated that these variants likely influence gene function through their effect on gene transcription. We replicated a strong association between CETP variants and HDL-C and between PCSK9 variant and LDL-C in West Africans, with two potentially functional variants and identified three novel variants in linkage disequilibrium in PON1 which were associated with increasing TC levels in Ghanaians.
Geert Lerouxroels - One of the best experts on this subject based on the ideXlab platform.
-
evaluation of the Automated haematology Analyser sysmex ne 8000
Clinical Chemistry and Laboratory Medicine, 1991Co-Authors: Katrien Devreese, E De Logi, Christiane Francart, B Heyndrickx, Jan Philippe, Geert LerouxroelsAbstract:the Automated Analyser routinely used in our laboratory, and with manual cell differentiation results. One hundred and seventy samples from the daily routine workload, comprising specimens from healthy adults and patients with various ailments, were analysed on the Sysmex NE-8000 and the Technicon H-l. A manual400 leukocyte differential count was performed on each specimen. Comparison of the results from the two blood cell counters showed good correlation (r > 0.9) for the white blood cell count, haemoglobin, haematocrit and platelet count. For the red blood cell count and mean cellular volume, the correlation coefficients were greater than 0.8. In the leukocyte differential count, Sysmex NE-8000 and Technicon H-l showed good correlations for the neutrophil (r = 0.953), lymphocyte (r = 0.763), and eosinophil counts (r = 0.904). Correlation coefficients were very low for monocyte (r = 0.130) and basophil counts (r = 0.006). Correlation l between the manual-400 method and the electronic leukocyte differential count showed similar results. \ Two hundred and twenty six normal and abnormal samples were compared with respect to morphology " flagging with the two Analysers, using the manual differentiation as the reference method. The abnormal specimens were representative of the range of leukoeyte abnormalities seen in our laboratory. Sensitivity for detecting blasts was equal for both Analysers. Sysmex NE-8000 was much more sensitive for detecting immature granulocytes than Technicon H-l. Low ranges of atypical lymphocytes were missed by Sysmex NE8000. Left shift was also frequently missed. During the evaluation period, Sysmex NE-8000 was very easy to handle and no Instrument malfunctions were met. The Sysmex NE-8000 is well suited for routine blood cell analysis and is a valuable tool for the diagnosis and screening of varioiis haematological abnormalities.
Marion Munster - One of the best experts on this subject based on the ideXlab platform.
-
evaluation of the sysmex xn 31 Automated Analyser for blood donor malaria screening at malawi blood transfusion services
Vox Sanguinis, 2021Co-Authors: Bridon Mbaya, Thom Mfune, Aubrey Samon, Talent Hwandih, Marion MunsterAbstract:BACKGROUND AND OBJECTIVES Balancing blood supply safety and sufficiency is challenging in malaria-endemic countries where the risk of transfusion-transmitted malaria (TTM) is ever-present. In support of reducing this risk, our study aimed at evaluating the performance of the Sysmex XN-31 Analyser in blood donor malaria screening, as compared with current practice in Malawi. MATERIALS AND METHODS This prospective observational study was conducted on remnant venous donor blood samples collected at Malawi Blood Transfusion Service donation sites countrywide for routine blood-borne pathogen screening. XN-31 results were compared with routine thick smear malaria microscopy, using expert microscopy (phase 1 and 2) plus qualitative malaria polymerase chain reaction (PCR) (phase 2) to adjudicate discrepancies. RESULTS XN-31 detected malaria in 614 (11.6%) of 5281 study samples compared with 341 (6.5%) for routine microscopy. Of the 273 discrepant samples, 60 smears (phase 1) could not be retrieved for expert microscopic review. Expert microscopy confirmed the XN-31 positivity in 78.8% (149/189) and 91.7% (22/24) of discrepant samples in phase 1 (n = 4416) and phase 2 (n = 975), respectively, with two cases requiring PCR testing, confirming one each as positive and negative, giving sensitivities of 100% and 75% and specificities of 99.9% and 100%, respectively, for XN-31 and routine microscopy. CONCLUSION The Automated Sysmex XN-31 Analyser's high sensitivity and specificity, ability to detect all Plasmodium species and high throughput with rapid turnaround-time, overcomes many of the limitations of currently available diagnostic tests, making it well-suited for malaria screening of donated blood in malaria-endemic countries in support of TTM risk reduction.
Sergei A. Eremin - One of the best experts on this subject based on the ideXlab platform.
-
Development of a polarization fluoroimmunoassay for sulfamethazine using an Automated Analyser
The Analyst, 1994Co-Authors: Sergei A. Eremin, John Landon, D S Smith, Roy JackmanAbstract:A rapid, non-isotopic, polarization fluoroimmunoassay for the antibiotic sulfamethazine has been developed. The influence of the structure of fluorescein-labelled tracers on the sensitivity of the assay was investigated. Reagents were adapted for use with the Abbott TDx Analyzer and existing software was adapted for Automated analyses. Total time for the assay of 1 and 20 samples was 14 and 22 min, respectively. All analytical criteria for the assay were satisfied. The detection limit for sulfamethazine in 20 µl of aqueous sample was 10 ng ml–1(200 pg), which compares well with the sensitivity of enzyme linked immunosorbent assay methods.
-
urinary cotinine fluoroimmunoassay for smoking status screening adapted to an Automated Analyser
Analyst, 1992Co-Authors: Sergei A. Eremin, Ruth Coxon, D L Colbert, J Landon, D S SmithAbstract:A polarization fluoroimmunoassay for cotinine, a major metabolite of nicotine, has been adapted for fully Automated screening of urine samples on the Abbott TDx Analyser. The method has sensitivity and specificity suitable for the discrimination of active smokers from non-smokers (including passive smokers) by application of a cut-off at 0.5 mg l–1 of total urinary cotinine. Most active smokers' urine gave results over 1 mg l–1, whereas apparent levels in non-smokers were 0.08 mg l–1 or lower. A result for one sample can be obtained in about 5 min and a throughput of 80 samples h–1 can be maintained for large-scale screening applications.
