The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform
Alan M. Jones - One of the best experts on this subject based on the ideXlab platform.
-
Altered Expression of Auxin-Binding Protein 1 Affects Cell Expansion and Auxin Pool Size in Tobacco Cells
Journal of Plant Growth Regulation, 2006Co-Authors: Jin-gui Chen, Richard M. Napier, Colin M. Lazarus, Shucai Wang, Alan M. JonesAbstract:Auxin-Binding Protein 1 (ABP1) has an essential role in Auxin-dependent cell expansion, but its mechanisms of action remain unknown. Our previous study showed that ABP1-mediated cell expansion is Auxin concentration dependent. However, Auxin distribution in plant tissue is heterogeneous, complicating the interpretation of ABP1 function. In this study, we used cells in culture that have altered expression of ABP1 to address the mechanism of ABP1 action at the cellular level, because cells in culture have homogeneous cell types and could potentially circumvent the heterogeneous Auxin-distributions inherent in plant tissues. We found that cells overexpressing ABP1 had altered sensitivity to Auxin and were larger, with nuclei that have undergone endoreduplication, a finding consistent with other data that support an Auxin extracellular receptor role for ABP1. These cells also had a higher free Auxin pool size, which cannot be explained by altered Auxin transport. In cells lacking detectable ABP1, a higher rate of Auxin metabolism was observed. The results suggest that ABP1 has, beyond its proposed role as an Auxin extracellular receptor, a role in mediating Auxin availability.
-
The role of Auxin‐Binding Protein 1 in the expansion of tobacco leaf cells
The Plant journal : for cell and molecular biology, 2002Co-Authors: Jin-gui Chen, Shoji Shimomura, Folke Sitbon, Göran Sandberg, Alan M. JonesAbstract:*Summary Tobacco leaf was used to investigate the mechanism of action of Auxin-Binding Protein 1 (ABP1). The distributions of free Auxin, ABP1, percentage of leaf nuclei in G2 and the amount of Auxin-inducible growth were each determined in control tobacco leaves and leaves over-expressing Arabidopsis ABP1. These parameters were compared with growth of tobacco leaves, measured both spatially and temporally throughout the entire expansion phase. Within a defined window of leaf development, juvenile leaf cells that inducibly expressed Arabidopsis ABP1 prematurely advanced nuclei to the G2 phase. The ABP1-induced increase in cell expansion occured before the advance to the G2 phase, indicating that the ABP1-induced G2 phase advance is an indirect effect of cell expansion. The level of ABP1 was highest at the position of maximum cell expansion, maximum Auxin-inducible growth and where the free Auxin level was the lowest. In contrast, the position of maximum cell division correlated with higher Auxin levels and lower ABP1 levels. Consistent with the correlations observed in leaves, tobacco cells (BY-2) in culture displayed two dose-dependent responses to Auxin. At a low Auxin concentration, cells expanded, while at a relatively higher concentration, cells divided and incorporated [ 3 H]-thymidine. Antisense suppression of ABP1 in these cells dramatically reduced cell expansion with negligible effect on cell division. Taken together, the data suggest that ABP1 acts at a relatively low level of Auxin to mediate cell expansion, whereas high Auxin levels stimulate cell division via an unidentified receptor.
-
the role of Auxin Binding Protein 1 in the expansion of tobacco leaf cells
Plant Journal, 2002Co-Authors: Jin-gui Chen, Shoji Shimomura, Folke Sitbon, Göran Sandberg, Alan M. JonesAbstract:*Summary Tobacco leaf was used to investigate the mechanism of action of Auxin-Binding Protein 1 (ABP1). The distributions of free Auxin, ABP1, percentage of leaf nuclei in G2 and the amount of Auxin-inducible growth were each determined in control tobacco leaves and leaves over-expressing Arabidopsis ABP1. These parameters were compared with growth of tobacco leaves, measured both spatially and temporally throughout the entire expansion phase. Within a defined window of leaf development, juvenile leaf cells that inducibly expressed Arabidopsis ABP1 prematurely advanced nuclei to the G2 phase. The ABP1-induced increase in cell expansion occured before the advance to the G2 phase, indicating that the ABP1-induced G2 phase advance is an indirect effect of cell expansion. The level of ABP1 was highest at the position of maximum cell expansion, maximum Auxin-inducible growth and where the free Auxin level was the lowest. In contrast, the position of maximum cell division correlated with higher Auxin levels and lower ABP1 levels. Consistent with the correlations observed in leaves, tobacco cells (BY-2) in culture displayed two dose-dependent responses to Auxin. At a low Auxin concentration, cells expanded, while at a relatively higher concentration, cells divided and incorporated [ 3 H]-thymidine. Antisense suppression of ABP1 in these cells dramatically reduced cell expansion with negligible effect on cell division. Taken together, the data suggest that ABP1 acts at a relatively low level of Auxin to mediate cell expansion, whereas high Auxin levels stimulate cell division via an unidentified receptor.
-
Auxin-Binding Protein mutants in maize.
Maydica, 2000Co-Authors: Jin-gui Chen, R. B. Meeley, Alan M. JonesAbstract:Maize Auxin-Binding Protein (ABP) is a putative Auxin receptor. However, its function has not been clearly demonstrated. To study loss-of-function of maize Abp1 and Ahp4, the two most highly expressed genes among the Abp multigene family in maize, we isolated insertion alleles of Robertson's Mutator. The loss-of-function of these mutants and the corresponding double mutants were confirmed by Northern and Western blot analyses. Despite the central importance of ABP supported in gain-of-function experiments, no obvious phenotypic aberration in these mutants was observed suggesting functional redundancy in this gene family in maize. In addition, compensatory mechanisms may be operating in maize because in abp4 loss-of-function mutants, ABP1 Protein level was significantly elevated.
-
Auxin-Dependent Cell Expansion Mediated by Overexpressed Auxin-Binding Protein 1
Science (New York N.Y.), 1998Co-Authors: Alan M. Jones, Michael A. Savka, N. Gregory Dewitt, Raymond D. Shillito, Andrew N. BinnsAbstract:To test the hypothesis that Auxin-Binding Protein 1 (ABP1) is a receptor controlling Auxin-mediated plant cell expansion, ABP1 complementary DNAs were expressed in a controllable fashion in tobacco plants and constitutively in maize cell lines. Induction of Arabidopsis ABP1 expression in tobacco leaf strips resulted in an increased capacity for Auxin-mediated cell expansion, whereas induction of ABP1 in intact plants resulted in leaves with a normal morphology, but larger cells. Similarly, constitutive expression of maize ABP1 in maize cell lines conferred on them the capacity to respond to Auxin by increasing cell size. These results support a role of ABP1 as an Auxin receptor controlling plant growth.
Alexandre Tromas - One of the best experts on this subject based on the ideXlab platform.
-
Auxin Binding Protein 1 is a negative regulator of the scf tir1 afb pathway
Nature Communications, 2013Co-Authors: Alexandre Tromas, Sébastien Paque, Vérène Stierlé, Anne-laure Quettier, Philippe Muller, Esther Lechner, Pascal Genschik, Catherine PerrotrechenmannAbstract:Auxin is a major plant hormone that controls most aspects of plant growth and development. Auxin is perceived by two distinct classes of receptors: transport inhibitor response 1 (TIR1, or Auxin-related F-box (AFB)) and Auxin/indole-3-acetic acid (AUX/IAA) coreceptors, that control transcriptional responses to Auxin, and the Auxin-Binding Protein 1 (ABP1), that controls a wide variety of growth and developmental processes. To date, the mode of action of ABP1 is still poorly understood and its functional interaction with TIR1/AFB-AUX/IAA coreceptors remains elusive. Here we combine genetic and biochemical approaches to gain insight into the integration of these two pathways. We find that ABP1 is genetically upstream of TIR1/AFBs; ABP1 knockdown leads to an enhanced degradation of AUX/IAA repressors, independently of its effects on endocytosis, through the SCF TIR1/AFB E3 ubiquitin ligase pathway. Combining positive and negative regulation of SCF ubiquitin-dependent pathways might be a common mechanism conferring tight control of hormone-mediated responses.
-
Auxin-Binding Protein 1 is a negative regulator of the SCF TIR1/AFB pathway
Nature communications, 2013Co-Authors: Alexandre Tromas, Sébastien Paque, Vérène Stierlé, Anne-laure Quettier, Philippe Muller, Esther Lechner, Pascal Genschik, Catherine Perrot-rechenmannAbstract:Auxin is a major plant hormone that controls most aspects of plant growth and development. Auxin is perceived by two distinct classes of receptors: transport inhibitor response 1 (TIR1, or Auxin-related F-box (AFB)) and Auxin/indole-3-acetic acid (AUX/IAA) coreceptors, that control transcriptional responses to Auxin, and the Auxin-Binding Protein 1 (ABP1), that controls a wide variety of growth and developmental processes. To date, the mode of action of ABP1 is still poorly understood and its functional interaction with TIR1/AFB-AUX/IAA coreceptors remains elusive. Here we combine genetic and biochemical approaches to gain insight into the integration of these two pathways. We find that ABP1 is genetically upstream of TIR1/AFBs; ABP1 knockdown leads to an enhanced degradation of AUX/IAA repressors, independently of its effects on endocytosis, through the SCF TIR1/AFB E3 ubiquitin ligase pathway. Combining positive and negative regulation of SCF ubiquitin-dependent pathways might be a common mechanism conferring tight control of hormone-mediated responses.
-
Auxin Binding Protein 1: functional and evolutionary aspects
Trends in plant science, 2010Co-Authors: Alexandre Tromas, Ivan A. Paponov, Catherine Perrot-rechenmannAbstract:In this review, we examine the role of Auxin Binding Protein 1 (ABP1) in mediating growth and developmental responses. ABP1 is involved in a broad range of cellular responses to Auxin, acting either as the main regulator of the response, such as seen for entry into cell division or, as a fine-tuning device as for the regulation of expression of early Auxin response genes. Phylogenetic analysis has revealed that ABP1 is an ancient Protein that was already present in various algae and has acquired a motif of retention in the endoplasmic reticulum only recently. An evaluation of the evidence for ABP1 function according to its cellular localization supports the plasma membrane as a starting point for ABP1-mediated Auxin signaling.
-
The Auxin Binding Protein 1 is required for differential Auxin responses mediating root growth.
PloS one, 2009Co-Authors: Alexandre Tromas, Klaus Palme, Ivan A. Paponov, Philippe Muller, Nils Braun, Tatyana Khodus, Karin Ljung, Ji-young Lee, Philip N. Benfey, James A. H. MurrayAbstract:Background In plants, the phytohormone Auxin is a crucial regulator sustaining growth and development. At the cellular level, Auxin is interpreted differentially in a tissue- and dose-dependent manner. Mechanisms of Auxin signalling are partially unknown and the contribution of the Auxin Binding Protein 1 (ABP1) as an Auxin receptor is still a matter of debate. Methodology/Principal Findings Here we took advantage of the present knowledge of the root biological system to demonstrate that ABP1 is required for Auxin response. The use of conditional ABP1 defective plants reveals that the Protein is essential for maintenance of the root meristem and acts at least on the D-type CYCLIN/RETINOBLASTOMA pathway to control entry into the cell cycle. ABP1 affects PLETHORA gradients and confers Auxin sensitivity to root cells thus defining the competence of the cells to be maintained within the meristem or to elongate. ABP1 is also implicated in the regulation of gene expression in response to Auxin. Conclusions/Significance Our data support that ABP1 is a key regulator for root growth and is required for Auxin-mediated responses. Differential effects of ABP1 on various Auxin responses support a model in which ABP1 is the major regulator for Auxin action on the cell cycle and regulates Auxin-mediated gene expression and cell elongation in addition to the already well known TIR1-mediated ubiquitination pathway.
Catherine Perrot-rechenmann - One of the best experts on this subject based on the ideXlab platform.
-
Auxin-Binding Protein 1 is a negative regulator of the SCF TIR1/AFB pathway
Nature communications, 2013Co-Authors: Alexandre Tromas, Sébastien Paque, Vérène Stierlé, Anne-laure Quettier, Philippe Muller, Esther Lechner, Pascal Genschik, Catherine Perrot-rechenmannAbstract:Auxin is a major plant hormone that controls most aspects of plant growth and development. Auxin is perceived by two distinct classes of receptors: transport inhibitor response 1 (TIR1, or Auxin-related F-box (AFB)) and Auxin/indole-3-acetic acid (AUX/IAA) coreceptors, that control transcriptional responses to Auxin, and the Auxin-Binding Protein 1 (ABP1), that controls a wide variety of growth and developmental processes. To date, the mode of action of ABP1 is still poorly understood and its functional interaction with TIR1/AFB-AUX/IAA coreceptors remains elusive. Here we combine genetic and biochemical approaches to gain insight into the integration of these two pathways. We find that ABP1 is genetically upstream of TIR1/AFBs; ABP1 knockdown leads to an enhanced degradation of AUX/IAA repressors, independently of its effects on endocytosis, through the SCF TIR1/AFB E3 ubiquitin ligase pathway. Combining positive and negative regulation of SCF ubiquitin-dependent pathways might be a common mechanism conferring tight control of hormone-mediated responses.
-
Auxin Binding Protein 1: functional and evolutionary aspects
Trends in plant science, 2010Co-Authors: Alexandre Tromas, Ivan A. Paponov, Catherine Perrot-rechenmannAbstract:In this review, we examine the role of Auxin Binding Protein 1 (ABP1) in mediating growth and developmental responses. ABP1 is involved in a broad range of cellular responses to Auxin, acting either as the main regulator of the response, such as seen for entry into cell division or, as a fine-tuning device as for the regulation of expression of early Auxin response genes. Phylogenetic analysis has revealed that ABP1 is an ancient Protein that was already present in various algae and has acquired a motif of retention in the endoplasmic reticulum only recently. An evaluation of the evidence for ABP1 function according to its cellular localization supports the plasma membrane as a starting point for ABP1-mediated Auxin signaling.
-
The Auxin-Binding Protein 1 is essential for the control of cell cycle.
Plant Journal, 2007Co-Authors: K.m. David, D. Couch, Nils Braun, Spencer Brown, Jeanne Grosclaude, Catherine Perrot-rechenmannAbstract:The phytohormone Auxin has been known for >50 years to be required for entry into the cell cycle. Despite the critical effects exerted by Auxin on the control of cell division, the molecular mechanism by which Auxin controls this pathway is poorly understood, and how Auxin is perceived upstream of any change in the cell cycle is unknown. Auxin Binding Protein 1 (ABP1) is considered to be a candidate Auxin receptor, triggering early modification of ion fluxes across the plasma membrane in response to Auxin. ABP1 has also been proposed to mediate Auxin-dependent cell expansion, and is essential for early embryonic development. We investigated whether ABP1 has a role in the cell cycle. Functional inactivation of ABP1 in the model plant cell system BY2 was achieved through cellular immunization via the conditional expression of a single-chain fragment variable (scFv). This scFv was derived from a well characterized anti-ABP1 monoclonal antibody previously shown to block the activity of the Protein. We demonstrate that functional inactivation of ABP1 results in cell-cycle arrest, and provide evidence that ABP1 plays a critical role in regulation of the cell cycle by acting at both the G1/S and G2/M checkpoints. We conclude that ABP1 is essential for the Auxin control of cell division and is likely to constitute the first step of the Auxin-signalling pathway mediating Auxin effects on the cell cycle.
-
A short history of Auxin-Binding Proteins.
Plant molecular biology, 2002Co-Authors: Richard M. Napier, Karine David, Catherine Perrot-rechenmannAbstract:Plant hormone receptors have proved to be elusive research targets. The successes of describing receptors from animals and bacteria have not yet been matched for plants. Nevertheless, where candidate receptors have been identified, they have been subjected to detailed examination. One such is the Protein known as ABP1, an Auxin-Binding Protein first described from maize (Zea mais L.).
-
The Auxin-Binding Protein Nt-ERabp1 alone activates an Auxin-like transduction pathway.
FEBS letters, 1999Co-Authors: Nathalie Leblanc, Catherine Perrot-rechenmann, Hélène Barbier-brygooAbstract:Hyperpolarization of tobacco protoplasts is amongst the earliest Auxin responses described. It has been proposed that the Auxin-Binding Protein, ABP1, or a related Protein could be involved in the first step of Auxin perception at the plasma membrane. Using for the first time homologous conditions for interaction between the Protein Nt-ERabp1 or a synthetic peptide corresponding to the C-terminus and tobacco protoplasts, we have demonstrated that both can induce the hyperpolarization response. The results show that Nt-ERabp1 or the C-terminal peptide alone activates the Auxin pathway from the outer face of the plasma membrane.
Catherine Perrotrechenmann - One of the best experts on this subject based on the ideXlab platform.
-
inhibition of cell expansion by rapid abp1 mediated Auxin effect on microtubules
Nature, 2014Co-Authors: Xu Chen, Sébastien Paque, Laurie Grandont, Robert Hauschild, Anas Abuzeineh, Hana Rakusova, Eva Benkova, Catherine PerrotrechenmannAbstract:The prominent and evolutionarily ancient role of the plant hormone Auxin is the regulation of cell expansion. Cell expansion requires ordered arrangement of the cytoskeleton but molecular mechanisms underlying its regulation by signalling molecules including Auxin are unknown. Here we show in the model plant Arabidopsis thaliana that in elongating cells exogenous application of Auxin or redistribution of endogenous Auxin induces very rapid microtubule re-orientation from transverse to longitudinal, coherent with the inhibition of cell expansion. This fast Auxin effect requires Auxin Binding Protein 1 (ABP1) and involves a contribution of downstream signalling components such as ROP6 GTPase, ROP-interactive Protein RIC1 and the microtubule-severing Protein katanin. These components are required for rapid Auxin- and ABP1-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses.
-
Auxin Binding Protein 1 is a negative regulator of the scf tir1 afb pathway
Nature Communications, 2013Co-Authors: Alexandre Tromas, Sébastien Paque, Vérène Stierlé, Anne-laure Quettier, Philippe Muller, Esther Lechner, Pascal Genschik, Catherine PerrotrechenmannAbstract:Auxin is a major plant hormone that controls most aspects of plant growth and development. Auxin is perceived by two distinct classes of receptors: transport inhibitor response 1 (TIR1, or Auxin-related F-box (AFB)) and Auxin/indole-3-acetic acid (AUX/IAA) coreceptors, that control transcriptional responses to Auxin, and the Auxin-Binding Protein 1 (ABP1), that controls a wide variety of growth and developmental processes. To date, the mode of action of ABP1 is still poorly understood and its functional interaction with TIR1/AFB-AUX/IAA coreceptors remains elusive. Here we combine genetic and biochemical approaches to gain insight into the integration of these two pathways. We find that ABP1 is genetically upstream of TIR1/AFBs; ABP1 knockdown leads to an enhanced degradation of AUX/IAA repressors, independently of its effects on endocytosis, through the SCF TIR1/AFB E3 ubiquitin ligase pathway. Combining positive and negative regulation of SCF ubiquitin-dependent pathways might be a common mechanism conferring tight control of hormone-mediated responses.
Philippe Muller - One of the best experts on this subject based on the ideXlab platform.
-
Auxin-Binding Protein 1 is a negative regulator of the SCF TIR1/AFB pathway
Nature communications, 2013Co-Authors: Alexandre Tromas, Sébastien Paque, Vérène Stierlé, Anne-laure Quettier, Philippe Muller, Esther Lechner, Pascal Genschik, Catherine Perrot-rechenmannAbstract:Auxin is a major plant hormone that controls most aspects of plant growth and development. Auxin is perceived by two distinct classes of receptors: transport inhibitor response 1 (TIR1, or Auxin-related F-box (AFB)) and Auxin/indole-3-acetic acid (AUX/IAA) coreceptors, that control transcriptional responses to Auxin, and the Auxin-Binding Protein 1 (ABP1), that controls a wide variety of growth and developmental processes. To date, the mode of action of ABP1 is still poorly understood and its functional interaction with TIR1/AFB-AUX/IAA coreceptors remains elusive. Here we combine genetic and biochemical approaches to gain insight into the integration of these two pathways. We find that ABP1 is genetically upstream of TIR1/AFBs; ABP1 knockdown leads to an enhanced degradation of AUX/IAA repressors, independently of its effects on endocytosis, through the SCF TIR1/AFB E3 ubiquitin ligase pathway. Combining positive and negative regulation of SCF ubiquitin-dependent pathways might be a common mechanism conferring tight control of hormone-mediated responses.
-
Auxin Binding Protein 1 is a negative regulator of the scf tir1 afb pathway
Nature Communications, 2013Co-Authors: Alexandre Tromas, Sébastien Paque, Vérène Stierlé, Anne-laure Quettier, Philippe Muller, Esther Lechner, Pascal Genschik, Catherine PerrotrechenmannAbstract:Auxin is a major plant hormone that controls most aspects of plant growth and development. Auxin is perceived by two distinct classes of receptors: transport inhibitor response 1 (TIR1, or Auxin-related F-box (AFB)) and Auxin/indole-3-acetic acid (AUX/IAA) coreceptors, that control transcriptional responses to Auxin, and the Auxin-Binding Protein 1 (ABP1), that controls a wide variety of growth and developmental processes. To date, the mode of action of ABP1 is still poorly understood and its functional interaction with TIR1/AFB-AUX/IAA coreceptors remains elusive. Here we combine genetic and biochemical approaches to gain insight into the integration of these two pathways. We find that ABP1 is genetically upstream of TIR1/AFBs; ABP1 knockdown leads to an enhanced degradation of AUX/IAA repressors, independently of its effects on endocytosis, through the SCF TIR1/AFB E3 ubiquitin ligase pathway. Combining positive and negative regulation of SCF ubiquitin-dependent pathways might be a common mechanism conferring tight control of hormone-mediated responses.
-
The Auxin Binding Protein 1 is required for differential Auxin responses mediating root growth.
PloS one, 2009Co-Authors: Alexandre Tromas, Klaus Palme, Ivan A. Paponov, Philippe Muller, Nils Braun, Tatyana Khodus, Karin Ljung, Ji-young Lee, Philip N. Benfey, James A. H. MurrayAbstract:Background In plants, the phytohormone Auxin is a crucial regulator sustaining growth and development. At the cellular level, Auxin is interpreted differentially in a tissue- and dose-dependent manner. Mechanisms of Auxin signalling are partially unknown and the contribution of the Auxin Binding Protein 1 (ABP1) as an Auxin receptor is still a matter of debate. Methodology/Principal Findings Here we took advantage of the present knowledge of the root biological system to demonstrate that ABP1 is required for Auxin response. The use of conditional ABP1 defective plants reveals that the Protein is essential for maintenance of the root meristem and acts at least on the D-type CYCLIN/RETINOBLASTOMA pathway to control entry into the cell cycle. ABP1 affects PLETHORA gradients and confers Auxin sensitivity to root cells thus defining the competence of the cells to be maintained within the meristem or to elongate. ABP1 is also implicated in the regulation of gene expression in response to Auxin. Conclusions/Significance Our data support that ABP1 is a key regulator for root growth and is required for Auxin-mediated responses. Differential effects of ABP1 on various Auxin responses support a model in which ABP1 is the major regulator for Auxin action on the cell cycle and regulates Auxin-mediated gene expression and cell elongation in addition to the already well known TIR1-mediated ubiquitination pathway.