The Experts below are selected from a list of 246 Experts worldwide ranked by ideXlab platform
Reginawanti Hindersah - One of the best experts on this subject based on the ideXlab platform.
-
Role and perspective of Azotobacter in crops production
SAINS TANAH - Journal of Soil Science and Agroclimatology, 2020Co-Authors: Reginawanti Hindersah, Nadia Nuraniya Kamaluddin, Suman Samanta, Saon Banerjee, Sarita SarkarAbstract:Low nitrogen content in soil is usually overcome by chemical fertilization. After long application period, high-dose and intensive use of N fertilizers can cause ammonia volatilization and nitrates accumulation in soil. In sustainable agriculture, the use of bacterial inoculant integrated with nutrient management system has a role in soil health and productivity. Azotobacter-based biofertilizer is suggested as a chemical nitrogen fertilizer substitute or addition in crop production to improve available nutrients in the soil, provide some metabolites during plant growth, and minimize fertilizer doses. The objective of this literature reviewed paper is to discuss the role of Azotobacter in agriculture; and the prospective of Azotobacter to increase yield and substitute the chemical fertilizer in food crops production. The results revealed that mechanisms by Azotobacter in plant growth enhancement are as biofertilizer, biostimulant, and bioprotectant. Nitrogen fixation by Azotobacter is the mechanism to provide available nitrogen for uptake by roots. Azotobacter stimulates plant growth through phytohormones synthesis; indole acetic acid, cytokinins, and gibberellins are detected in the liquid culture of Azotobacter. An indirect effect of Azotobacter is exopolysaccharide production and plant protection. Inoculation of Azotobacter in the field integrated with organic matter and reduced chemical fertilizer are reported to improve plant growth and yield.
-
Antibiotic Resistance of Azotobacter Isolated from Mercury-Contaminated Area
Journal of Agricultural Studies, 2019Co-Authors: Reginawanti Hindersah, Gina Nurhabibah, Priyanka Asmiran, Etty PratiwiAbstract:Nitrogen-fixing Azotobacter is a renewable source of biofertilizer for plant growth. Increased of antibiotic level in soil due to intensive used manure is believed to induce bacterial sensitivity to antaibiotic. An antibiotic sensitivity test has been carried out to study the inhibition effect of ampicillin, streptomycin, tetracycline and chloramphenicol on Azotobacter isolated from mercury-contaminated taling. The resistance test was performend by using disc plate method in Nitrogen-free Ashby’s agar with and without mercury. The results showed that the presence of 20 mg/L mercury in plate agar totally inhibited Azotobater growth. In the absence of mercury chloride, all isolates showed different sensitivity to antibiotics. Growth of Azotobacter buru1 was only inhibited by tetracycline. Azotobacter buru2 was susceptible to high and low concentration of tetracycline and streptomycin but they were resistance to low concentration of chloramphenicol as well as ampicillin; while Azotobacter bd3a were sensitive to all tested antibiotic. In conclusion, order of Azotobacter resistance to antibiotics in the absence of mercury was Bd3a<Buru2<Buru1. This research have not revealed the resistance of Azotobacter to antibiotic in the presence of mercury.
-
Antibiotic Resistance of Azotobacter Isolated from Mercury-Contaminated Area
Journal of Animal Science, 2019Co-Authors: Reginawanti Hindersah, Gina Nurhabibah, Priyanka Asmiran, Etty PratiwiAbstract:Nitrogen-fixing Azotobacter is a renewable source of biofertilizer for plant growth. Increased of antibiotic level in soil due to intensive used manure is believed to induce bacterial sensitivity to antaibiotic. An antibiotic sensitivity test has been carried out to study the inhibition effect of ampicillin, streptomycin, tetracycline and chloramphenicol on Azotoba c ter isolated from mercury-contaminated taling. The resistance test was performend by using disc plate method in Nitrogen-free Ashby’s agar with and without mercury. The results showed that the presence of 20 mg/L mercury in plate agar totally inhibited Azotobater growth. In the absence of mercury chloride, all isolates showed different sensitivity to antibiotics. Growth of Azotobacter buru1 was only inhibited by tetracycline. Azotobacter buru2 was susceptible to high and low concentration of tetracycline and streptomycin but they were resistance to low concentration of chloramphenicol as well as ampicillin; while Azotobacter bd3a were sensitive to all tested antibiotic. In conclusion, order of Azotobacter resistance to antibiotics in the absence of mercury was Bd3a
-
NITROGEN FIXING BACTERIA Azotobacter AS BIOFERTILIZER AND BIOCONTROL IN LONG BEAN
Agric, 2018Co-Authors: Reginawanti Hindersah, Marthin Kalay, Abraham Talahaturuson, Yansen LakburlawalAbstract:Azotobacter is Plant Growth Promoting Rhizobacteria through the mechanism of nitrogen fixation and phytohormon production but this rhizobacteria has a role to control plant diseases. The objective of experiment was to evaluate the activity of Azotobacter as biofertilizers as well as biocontrol on long bean cultivation in damping off endemic land in Ambon city, Maluku Province. The field experiment was arranged in completely randomized block design. Inoculation of long bean by Azotobacter has been done by seed inoculation, soil inoculation before planting, and plant inoculation. Plants treated with Azotobacter received fertilizer NPK of ¾ or ½ dosage recommendation while control plants were received 100% NPK. Research showed that no differences between yield of long bean inoculated with Azotobacter sp +reduced doses of NPK with that of control plants. Any application method of Azotobacter inoculation lowered damping off diseases incidence significantly until 10 days after planting, but no effect of inoculation on late blight at 21 days after planting. This study confirmed that Azotobacter has dual activity to reduce the dose of NPK fertilizer and control damping off.
-
Azotobacter population, soil nitrogen and groundnut growth in mercury-contaminated tailing inoculated with Azotobacter
Journal of Degraded and Mining Lands Management, 2018Co-Authors: Reginawanti Hindersah, Zellya Handyman, Febby Nur Indriani, Pujawati Suryatmana, Nenny NurlaenyAbstract:Gold mine tailing in Buru island, Maluku Province, Indonesia contains high level of mercury but low in carbon and plant nutrients. Revegetation in such condition needs certain soil treatment which is suitable for plant growth. The objective of pot trials was to study the effect of indigenous Plant Growth Promoting Rhizobacteria Azotobacter and organic matter on bacterial survival as well as growth of groundnut grown in mine tailing. The experimental design was a Split Plot Design which tested three types of Azotobacter liquid inoculant and three soil total organic carbon (TOC) contents. Results showed that Azotobacter inoculation increased Azotobacter population in tailing at the end of vegetative growth of groundnut. Total nitrogen content in soil decreased when TOC level increased. However, nitrogen uptake and growth of groundnut did not change after Azotobacter inoculation or manure amendment. These experiments provided information that Azotobacter inoculation on groundnut maintain its proliferation in Hg-contaminated mine tailing.
Bikas R. Pati - One of the best experts on this subject based on the ideXlab platform.
-
Impact of Azotobacter exopolysaccharides on sustainable agriculture
Applied microbiology and biotechnology, 2012Co-Authors: Samiran S. Gauri, Santi M. Mandal, Bikas R. PatiAbstract:Recently, increasing attention have lead to search other avenue of biofertilizers with multipurpose activities as a manner of sustainable soil health to improve the plant productivity. Azotobacter have been universally accepted as a major inoculum used in biofertilizer to restore the nitrogen level into cultivated field. Azotobacter is well characterized for their profuse production of exopolysaccharides (EPS). Several reviews on biogenesis and multifunctional role of Azotobacter EPS have been documented with special emphasis on industrial applications. But the impact of Azotobacter EPS in plant growth promotion has not received adequate attention. This review outlines the evidence that demonstrates not only the contribution of Azotobacter EPS in global nutrient cycle but also help to compete successfully in different adverse ecological and edaphic conditions. This also focuses on new insights and concepts of Azotobacter EPS which have positive effects caused by the biofilm formation on overall plant growth promotion with other PGPRs. In addition, their potentials in agricultural improvement are also discussed. Recent data realized that Azotobacter EPS have an immense agro-economical importance including the survivability and maintenance of microbial community in their habitat. This leads us to confirm that the next generation Azotobacter inoculum with high yielding EPS and high nitrogen fixing ability can be utilized to satisfy the future demand of augmented crop production attributed to increase plant growth promoting agents.
Appa Rao Podile - One of the best experts on this subject based on the ideXlab platform.
-
transgenic expression of glucose dehydrogenase in Azotobacter vinelandii enhances mineral phosphate solubilization and growth of sorghum seedlings
Microbial Biotechnology, 2009Co-Authors: Burla Sashidhar, Appa Rao PodileAbstract:The enzyme quinoprotein glucose dehydrogenase (GDH) catalyses the oxidation of glucose to gluconic acid by direct oxidation in the periplasmic space of several Gram‐negative bacteria. Acidification of the external environment with the release of gluconic acid contributes to the solubilization of the inorganic phosphate by biofertilizer strains of the phosphate‐solubilizing bacteria. Glucose dehydrogenase (gcd) gene from Escherichia coli, and Azotobacter‐specific glutamine synthetase (glnA) and phosphate transport system (pts) promoters were isolated using sequence‐specific primers in a PCR‐based approach. Escherichia coli gcd, cloned under the control of glnA and pts promoters, was mobilized into Azotobacter vinelandii AvOP and expressed. Sorghum seeds were bacterized with the transgenic Azotobacters and raised in earthen pots in green house. The transgenic Azotobacters, expressing E. coli gcd, showed improved biofertilizer potential in terms of mineral phosphate solubilization and plant growth‐promoting activity with a small reduction in nitrogen fixation ability.
L D B Suriyagoda - One of the best experts on this subject based on the ideXlab platform.
-
evaluating the growth performance of two azotobactor spp in liquid glucose broth and tryptic peptone broth as inoculum for the production of bio fertilizers
Tropical agricultural research, 2017Co-Authors: A D Nagalla, D V Jayatilake, Venura Herath, L D B SuriyagodaAbstract:Biofertilizers with Azotobacter spp. releases available nitrogen to the plant rhizosphere. A media that yield a high viable inoculum cell count in a shorter incubation period is important for commercial production of biofertilizers. Current study evaluates the growth performances of Azotobacter chroococcum and Azotobacter vinelandii, two spp. commonly found in sugarcane rhizosphere, in two broth cultures: tryptic peptone broth (TPB) and liquid glucose broth (LGB) using optical density (OD) measurements at a wavelength of 620 nm over an incubation period of 12 - 15 hours. The analysis of variance revealed significant (p<0.001) species, broth and species × broth interaction on OD. Azotobacter spp. had a higher growth rate in the protein enriched TPB compared to LGB. Azotobacter chroococcum had a higher growth rate than A. vinelandii particularly in TPB. Despite the cost, A. chroococcum inoculum grown in TPB can be recommended for commercial biofertilizer production, due to yield and time advantage.
-
Evaluating the growth performance of Two Azotobactor spp. In liquid glucose broth and tryptic peptone broth as inoculum for the production of bio-fertilizers
Tropical Agricultural Research, 2017Co-Authors: A D Nagalla, D V Jayatilake, Venura Herath, L D B SuriyagodaAbstract:Biofertilizers with Azotobacter spp. releases available nitrogen to the plant rhizosphere. A media that yield a high viable inoculum cell count in a shorter incubation period is important for commercial production of biofertilizers. Current study evaluates the growth performances of Azotobacter chroococcum and Azotobacter vinelandii, two spp. commonly found in sugarcane rhizosphere, in two broth cultures: tryptic peptone broth (TPB) and liquid glucose broth (LGB) using optical density (OD) measurements at a wavelength of 620 nm over an incubation period of 12 - 15 hours. The analysis of variance revealed significant (p
Samiran S. Gauri - One of the best experts on this subject based on the ideXlab platform.
-
Impact of Azotobacter exopolysaccharides on sustainable agriculture
Applied microbiology and biotechnology, 2012Co-Authors: Samiran S. Gauri, Santi M. Mandal, Bikas R. PatiAbstract:Recently, increasing attention have lead to search other avenue of biofertilizers with multipurpose activities as a manner of sustainable soil health to improve the plant productivity. Azotobacter have been universally accepted as a major inoculum used in biofertilizer to restore the nitrogen level into cultivated field. Azotobacter is well characterized for their profuse production of exopolysaccharides (EPS). Several reviews on biogenesis and multifunctional role of Azotobacter EPS have been documented with special emphasis on industrial applications. But the impact of Azotobacter EPS in plant growth promotion has not received adequate attention. This review outlines the evidence that demonstrates not only the contribution of Azotobacter EPS in global nutrient cycle but also help to compete successfully in different adverse ecological and edaphic conditions. This also focuses on new insights and concepts of Azotobacter EPS which have positive effects caused by the biofilm formation on overall plant growth promotion with other PGPRs. In addition, their potentials in agricultural improvement are also discussed. Recent data realized that Azotobacter EPS have an immense agro-economical importance including the survivability and maintenance of microbial community in their habitat. This leads us to confirm that the next generation Azotobacter inoculum with high yielding EPS and high nitrogen fixing ability can be utilized to satisfy the future demand of augmented crop production attributed to increase plant growth promoting agents.
