Babesia gibsoni

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Xuenan Xuan - One of the best experts on this subject based on the ideXlab platform.

  • Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni.
    Parasites & vectors, 2018
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Patrick Vudriko, Hassan Hakimi, Tatsunori Masatani, Shin-ichiro Kawazu, Seung-hun Lee, Junya Yamagishi, Xuenan Xuan
    Abstract:

    Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis. GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis. We present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models.

  • Additional file 1: of Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
    2018
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Patrick Vudriko, Hassan Hakimi, Tatsunori Masatani, Shin-ichiro Kawazu, Seung-hun Lee, Junya Yamagishi, Xuenan Xuan
    Abstract:

    Figure S1. Babesia gibsoni sensitivity to WR99210. All data are expressed as means Âą SD of triplicate cultures. (PPTX 100 kb

  • Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
    BMC, 2018
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Patrick Vudriko, Hassan Hakimi, Tatsunori Masatani, Shin-ichiro Kawazu, Seung-hun Lee, Junya Yamagishi, Xuenan Xuan
    Abstract:

    Abstract Background Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis. Results GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis. Conclusions We present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models

  • Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across japan
    Journal of Veterinary Medical Science, 2016
    Co-Authors: Mingming Liu, Shinuo Cao, Patrick Vudriko, Hiroshi Suzuki, Takehisa Soma, Xuenan Xuan
    Abstract:

    Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2-100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.

  • The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan.
    The Journal of veterinary medical science, 2015
    Co-Authors: Aiko Iguchi, Hiroshi Suzuki, Takehisa Soma, Xuenan Xuan
    Abstract:

    In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.

Hiroshi Suzuki - One of the best experts on this subject based on the ideXlab platform.

  • Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across japan
    Journal of Veterinary Medical Science, 2016
    Co-Authors: Mingming Liu, Shinuo Cao, Patrick Vudriko, Hiroshi Suzuki, Takehisa Soma, Xuenan Xuan
    Abstract:

    Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2-100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.

  • The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan.
    The Journal of veterinary medical science, 2015
    Co-Authors: Aiko Iguchi, Hiroshi Suzuki, Takehisa Soma, Xuenan Xuan
    Abstract:

    In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.

  • Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni.
    Veterinary parasitology, 2012
    Co-Authors: Youn-kyoung Goo, Hiroshi Suzuki, Gabriel Oluga Aboge, Yoshifumi Nishikawa, Mohamad Alaa Terkawi, Suk Kim, Naeun Lee, Yuzi Luo, Xuenan Xuan
    Abstract:

    We developed an immunochromatographic test (ICT) with the full-length of thrombospondin-related adhesive protein of Babesia gibsoni expressed by the modified expression method. The developed ICT showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ICT could be a new practical diagnostic test for B. gibsoni infection.

  • Isolation and Identification of an Actin Gene From Babesia gibsoni
    The Journal of parasitology, 2006
    Co-Authors: Jinlin Zhou, Ikuo Igarashi, Kozo Fujisaki, Hiroshi Suzuki, Bing Huang, Xuenan Xuan
    Abstract:

    Actin is a ubiquitous and highly conserved microfilament protein that is hypothesized to play a mechanical force-generating role in the unusual gliding motility of sporozoan zoites and their active penetration of host cells. We have identified and isolated an actin gene from a Babesia gibsoni cDNA library by random sequencing. The complete nucleotide sequence of the actin gene is 1,243 bp; a single open reading frame encodes a polypeptide of 377 amino acid residues. The deduced amino acid sequence showed a high homology with actins from other species, especially with reported apicomplexan protozoans. The antiserum against recombinant actin expressed in Escherichia coli recognizes a 42-kDa native protein, which is consistent with its expected size. Immunofluorescence and confocal microscopic observation revealed that the protein is diffusely distributed throughout the B. gibsoni parasites.

  • fatal experimental transplacental Babesia gibsoni infections in dogs
    International Journal for Parasitology, 2005
    Co-Authors: Shinya Fukumoto, Ikuo Igarashi, Hiroshi Suzuki, Xuenan Xuan
    Abstract:

    A Babesia gibsoni infected bitch was mated with an uninfected dog in order to determine whether this parasite could be vertically transmitted. The bitch delivered a litter of four live and one stillborn pup. The four pups died from congenital babesiosis between 14 and 39 days post-birth. Babesia gibsoni DNA was detected in tissue from all five pups. These results show that vertical transmission occurred by the uterine route and not via the transmammary route. This is the first confirmed report of transplacental Babesia infection in any animal species.

Mingming Liu - One of the best experts on this subject based on the ideXlab platform.

  • Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni.
    Parasites & vectors, 2018
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Patrick Vudriko, Hassan Hakimi, Tatsunori Masatani, Shin-ichiro Kawazu, Seung-hun Lee, Junya Yamagishi, Xuenan Xuan
    Abstract:

    Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis. GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis. We present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models.

  • Additional file 1: of Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
    2018
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Patrick Vudriko, Hassan Hakimi, Tatsunori Masatani, Shin-ichiro Kawazu, Seung-hun Lee, Junya Yamagishi, Xuenan Xuan
    Abstract:

    Figure S1. Babesia gibsoni sensitivity to WR99210. All data are expressed as means Âą SD of triplicate cultures. (PPTX 100 kb

  • Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
    BMC, 2018
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Patrick Vudriko, Hassan Hakimi, Tatsunori Masatani, Shin-ichiro Kawazu, Seung-hun Lee, Junya Yamagishi, Xuenan Xuan
    Abstract:

    Abstract Background Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis. Results GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis. Conclusions We present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models

  • Transient transfection of intraerythrocytic Babesia gibsoni using elongation factor-1 alpha promoter.
    Molecular and biochemical parasitology, 2017
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Shinuo Cao, Patrick Vudriko, Artemis Efstratiou, Hassan Hakimi, Tatsunori Masatani, Fujiko Sunaga, Shin-ichiro Kawazu
    Abstract:

    The development of gene manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not yet been established for Babesia gibsoni. Here, we report for the first time, the successful transient transfection of B. gibsoni. The plasmid containing the firefly luciferase reporter gene (pBS-ELA) was transfected into B. gibsoni by an AMAXA 4D Nucleofector™ device. Transfection using program FA113 and Lonza buffer SF showed the highest luciferase expression. Twenty micrograms of plasmid produced the highest relative transfection efficiency. The fluorescent protein-expressing parasites were determined by GFP-containing plasmid (pBS-EGA) at 48 and 72h post transfection. This finding is the first step towards a stable transfection method for B. gibsoni, which may contribute to a better understanding of the biology of the parasite.

  • Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across japan
    Journal of Veterinary Medical Science, 2016
    Co-Authors: Mingming Liu, Shinuo Cao, Patrick Vudriko, Hiroshi Suzuki, Takehisa Soma, Xuenan Xuan
    Abstract:

    Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2-100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.

Patrick Vudriko - One of the best experts on this subject based on the ideXlab platform.

  • Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni.
    Parasites & vectors, 2018
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Patrick Vudriko, Hassan Hakimi, Tatsunori Masatani, Shin-ichiro Kawazu, Seung-hun Lee, Junya Yamagishi, Xuenan Xuan
    Abstract:

    Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis. GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis. We present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models.

  • Additional file 1: of Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
    2018
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Patrick Vudriko, Hassan Hakimi, Tatsunori Masatani, Shin-ichiro Kawazu, Seung-hun Lee, Junya Yamagishi, Xuenan Xuan
    Abstract:

    Figure S1. Babesia gibsoni sensitivity to WR99210. All data are expressed as means Âą SD of triplicate cultures. (PPTX 100 kb

  • Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
    BMC, 2018
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Patrick Vudriko, Hassan Hakimi, Tatsunori Masatani, Shin-ichiro Kawazu, Seung-hun Lee, Junya Yamagishi, Xuenan Xuan
    Abstract:

    Abstract Background Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis. Results GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis. Conclusions We present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models

  • Transient transfection of intraerythrocytic Babesia gibsoni using elongation factor-1 alpha promoter.
    Molecular and biochemical parasitology, 2017
    Co-Authors: Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Shinuo Cao, Patrick Vudriko, Artemis Efstratiou, Hassan Hakimi, Tatsunori Masatani, Fujiko Sunaga, Shin-ichiro Kawazu
    Abstract:

    The development of gene manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not yet been established for Babesia gibsoni. Here, we report for the first time, the successful transient transfection of B. gibsoni. The plasmid containing the firefly luciferase reporter gene (pBS-ELA) was transfected into B. gibsoni by an AMAXA 4D Nucleofector™ device. Transfection using program FA113 and Lonza buffer SF showed the highest luciferase expression. Twenty micrograms of plasmid produced the highest relative transfection efficiency. The fluorescent protein-expressing parasites were determined by GFP-containing plasmid (pBS-EGA) at 48 and 72h post transfection. This finding is the first step towards a stable transfection method for B. gibsoni, which may contribute to a better understanding of the biology of the parasite.

  • Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across japan
    Journal of Veterinary Medical Science, 2016
    Co-Authors: Mingming Liu, Shinuo Cao, Patrick Vudriko, Hiroshi Suzuki, Takehisa Soma, Xuenan Xuan
    Abstract:

    Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2-100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.

Youn-kyoung Goo - One of the best experts on this subject based on the ideXlab platform.

  • New Molecules in Babesia gibsoni and their application for diagnosis, vaccine development, and drug discovery.
    The Korean journal of parasitology, 2014
    Co-Authors: Youn-kyoung Goo, Xuenan Xuan
    Abstract:

    Babesia gibsoni is an intraerythrocytic apicomplexan parasite that causes piroplasmosis in dogs. B. gibsoni infection is characterized clinically by fever, regenerative anemia, splenomegaly, and sometimes death. Since no vaccine is available, rapid and accurate diagnosis and prompt treatment of infected animals are required to control this disease. Over the past decade, several candidate molecules have been identified using biomolecular techniques in the authors' laboratory for the development of a serodiagnostic method, vaccine, and drug for B. gibsoni. This review article describes newly identified candidate molecules and their applications for diagnosis, vaccine production, and drug development of B. gibsoni.

  • Actin polymerization mediated by Babesia gibsoni aldolase is required for parasite invasion.
    Experimental parasitology, 2013
    Co-Authors: Youn-kyoung Goo, Mohamad Alaa Terkawi, Akio Ueno, G. Oluga Aboge, Yamagishi Junya, Makoto Igarashi, Jungyeon Kim, Yeonchul Hong, Dong-il Chung, Yoshifumi Nishikawa
    Abstract:

    Host cell invasion by apicomplexan parasites driven by gliding motility and empowered by actin-based movement is essential for parasite survival and pathogenicity. The parasites share a conserved invasion process: actin-based motility led by the coordination of adhesin-cytoskeleton via aldolase. A number of studies of host cell invasion in the Plasmodium species and Toxoplasma gondii have been performed. However, the mechanisms of host cell invasion by Babesia species have not yet been studied. Here, we show that Babesia gibsoni aldolase (BgALD) forms a complex with B. gibsoni thrombospondin-related anonymous protein (BgTRAP) and B. gibsoni actin (BgACT), depending on tryptophan-734 (W-734) in BgTRAP. In addition, actin polymerization is mediated by BgALD. Moreover, cytochalasin D, which disrupts actin polymerization, suppressed B. gibsoni parasite growth and inhibited the host cell invasion by parasites, indicating that actin dynamics are essential for erythrocyte invasion by B. gibsoni. This study is the first molecular approach to determine the invasion mechanisms of Babesia species.

  • Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni.
    Veterinary parasitology, 2012
    Co-Authors: Youn-kyoung Goo, Hiroshi Suzuki, Gabriel Oluga Aboge, Yoshifumi Nishikawa, Mohamad Alaa Terkawi, Suk Kim, Naeun Lee, Yuzi Luo, Xuenan Xuan
    Abstract:

    We developed an immunochromatographic test (ICT) with the full-length of thrombospondin-related adhesive protein of Babesia gibsoni expressed by the modified expression method. The developed ICT showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ICT could be a new practical diagnostic test for B. gibsoni infection.

  • Babesia gibsoni: identification, expression, localization, and serological characterization of a Babesia gibsoni 22-kDa protein.
    Experimental parasitology, 2009
    Co-Authors: Youn-kyoung Goo, Junya Yamagishi, Kozo Fujisaki, Honglin Jia, Gabriel Oluga Aboge, Yoshifumi Nishikawa, Mohamad Alaa Terkawi, Suk Kim, Hyung-kwan Jang, Xuenan Xuan
    Abstract:

    Babesia gibsoni causes canine babesiosis. Here, we describe the identification and characterization of a novel gene, BgP22, containing an open reading frame of 621 bp and encoding a 22-kDa protein from B. gibsoni, as a serodiagnostic candidate. The recombinant BgP22 (rBgP22) was expressed and used as an antigen to produce anti-rBgP22 sera in mice. Using these anti-rBgP22 sera, a native 22-kDa protein was recognized by Western blot analysis and observed in the membrane of the parasites by immunofluorescent antibody tests (IFAT). The enzyme-linked immunosorbent assay (ELISA) using the rBgP22 detected specific antibodies to this protein in the sera of dogs experimentally and naturally infected with B. gibsoni in chronic stage. Furthermore, it did not show a cross reaction with the closely related apicomplexan parasites, indicating that the rBgP22 could be used as a diagnostic antigen for a detection of the chronic carrier stages of B. gibsoni infection.

  • Identification of Secreted Antigen 3 from Babesia gibsoni
    Clinical and vaccine immunology : CVI, 2009
    Co-Authors: Honglin Jia, Ikuo Igarashi, Kozo Fujisaki, M. Alaa Terkawi, Gabriel Oluga Aboge, Youn-kyoung Goo, Jinlin Zhou, Yoshifumi Nishikawa, Xuenan Xuan
    Abstract:

    A cDNA expression library of Babesia gibsoni was screened with the serum collected from a dog experimentally infected with B. gibsoni. A novel antigen sharing homology with secreted antigen 1 of B. gibsoni was isolated. The genomic analysis indicated that the BgSA3 gene exists as multicopies in the genome of B. gibsoni. The putative peptide encoded by the BgSA3 gene showed some characteristics of secreted proteins. The serum raised in mice immunized with the recombinant BgSA3 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 70 kDa. Moreover, a sandwich enzyme-linked immunosorbent assay with anti-BgSA3 antibodies could detect the circulating BgSA3 in the blood plasma of dogs experimentally infected with B. gibsoni. The identification of BgSA3 provided a useful target for the development of a diagnostic test for detecting specific antibodies and circulating antigens.