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Elma Tchilian - One of the best experts on this subject based on the ideXlab platform.
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Identification of a Newly Conserved SLA-II Epitope in a Structural Protein of Swine Influenza Virus.
Frontiers in immunology, 2020Co-Authors: Massimiliano Baratelli, Johanneke D. Hemmink, Bryan Charleston, Sophie B. Morgan, Elizabeth Reid, Brigid Veronica Carr, Eric A. Lefevre, Sergio Montaner-tarbes, Lorenzo Fraile, Elma TchilianAbstract:Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs.
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The major histocompatibility complex homozygous inbred Babraham pig as a resource for veterinary and translational medicine.
HLA, 2018Co-Authors: John C. Schwartz, Johanneke D. Hemmink, Simon P. Graham, Elma Tchilian, Bryan Charleston, Sabine E. Hammer, John A. HammondAbstract:The Babraham pig is a highly inbred breed first developed in the United Kingdom approximately 50 years ago. Previous reports indicate a very high degree of homozygosity across the genome, including the major histocompatibility complex (MHC) region, but confirmation of homozygosity at the specific MHC loci was lacking. Using both direct sequencing and PCR‐based sequence‐specific typing, we confirm that Babraham pigs are essentially homozygous at their MHC loci and formalise their MHC haplotype as Hp‐55.6. This enhances the utility of the Babraham pig as a useful biomedical model for studies in which controlling for genetic variation is important.
Michael H. Abraham - One of the best experts on this subject based on the ideXlab platform.
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comments on classification of biphasic solvent systems according to abraham descriptors for countercurrent chromatography
Journal of Chromatography A, 2020Co-Authors: William E. Acree, Brittani Churchill, Michael H. AbrahamAbstract:Abraham model correlations reported by Marlot and coworkers for the 1-octanol/water, 1-butanol/water, ethyl acetate/water, and heptane/methanol biphasic partitioning systems are compared to previously published Abraham model correlations. The previously published correlations for the fore-mentioned partitioning systems are based on more experimental data points, and exhibit much better descriptive ability as evidenced by much smaller standard deviations/standard errors and larger squared correlation coefficients.
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The partition of organic compounds from water into the methyl isobutyl ketone extraction solvent with updated Abraham model equation
Physics and Chemistry of Liquids, 2020Co-Authors: Katherine Smart, William E. Acree, Estefania Garcia, Blessing Oloyede, Rachel Fischer, Teresa D. Golden, Michael H. AbrahamAbstract:Experimental water-to-methyl isobutyl ketone partition coefficients have been determined for 27 different organic solutes based on gas chromatographic measurements. Updated Abraham model correlatio...
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Determination of Abraham Model Correlations for Solute Transfer into Propyl Acetate Based on Experimental Activity Coefficient and Solubility Data
Journal of Solution Chemistry, 2018Co-Authors: Igor A Sedov, Timur M. Salikov, Diliara R. Khaibrakhmanova, Anisha Wadawadigi, Olivia Zha, Ellen Qian, Maribel Barrera, Erin Hart, William Eugence Acree, Michael H. AbrahamAbstract:Experimental infinite dilution activity coefficients, gas-to-liquid partition coefficients, and molar solubility data have been measured for numerous organic solutes dissolved in propyl acetate. Results of our experimental measurements, combined with published solubility data retrieved from the published literature, have been used to derive Abraham model correlations for describing solute transfer into propyl acetate. The derived Abraham model correlations describe the experimental data to within 0.11 log_10 units. Calculation of Abraham model solute descriptors for boscalid was illustrated using our derived solute transfer correlations into propyl acetate. Predictions using the calculated solute descriptors indicate that boscalid would show significant partitioning into the skin and fat tissues in the body, and would exhibit considerable baseline toxicity towards the eight aquatic organisms (five fish species and three water flea species).
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Abraham model expressions for describing water-to-organic solvent and gas-to-organic solvent partition coefficients for solute transfer into anhydrous poly(ethylene glycol) dialkyl ether solvents at 298.15 K
Physics and Chemistry of Liquids, 2016Co-Authors: Erin Hart, William E. Acree, Sarah Cheeran, Grace E. Little, Hunter Singleton, Michael H. AbrahamAbstract:ABSTRACTHenry’s law constants and infinite dilution activity coefficients were compiled from the published chemical and engineering literature for gaseous solutes and organic liquids in butyl diglyme, butyl triglyme and tetraglyme. The published literature values were converted into water-to-liquid and gas-to-liquid partition coefficients using standard thermodynamic relationships. The calculated partition coefficients were correlated mathematically with the Abraham solvation parameter model. The derived Abraham model correlations can be used to predict the partitioning behaviour of additional solutes into dry, anhydrous butyl diglyme, butyl triglyme and tetraglyme.
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Determination of Abraham model solute descriptors for the monomeric and dimeric forms of trans-cinnamic acid using measured solubilities from the Open Notebook Science Challenge
Chemistry Central Journal, 2015Co-Authors: Jean-claude Bradley, Andrew Sid Lang, Samantha N Beck, David A Bulger, Lacey N Condron, Stephanie T Costa, Elizabeth A Clark, Michael H. Abraham, William E. Acree, Evan M CurtinAbstract:Background Calculating Abraham descriptors from solubility values requires that the solute have the same form when dissolved in all solvents. However, carboxylic acids can form dimers when dissolved in non-polar solvents. For such compounds Abraham descriptors can be calculated for both the monomeric and dimeric forms by treating the polar and non-polar systems separately. We illustrate the method of how this can be done by calculating the Abraham descriptors for both the monomeric and dimeric forms of trans-cinnamic acid, the first time that descriptors for a carboxylic acid dimer have been obtained. Results Abraham descriptors were calculated for the monomeric form of trans-cinnamic acid using experimental solubility measurements in polar solvents from the Open Notebook Science Challenge together with a number of water-solvent partition coefficients from the literature. Similarly, experimental solubility measurements in non-polar solvents were used to determine Abraham descriptors for the trans-cinnamic acid dimer. Conclusion Abraham descriptors were calculated for both the monomeric and dimeric forms of trans-cinnamic acid. This allows for the prediction of further solubilities of trans-cinnamic acid in both polar and non-polar solvents with an error of about 0.10 log units. Graphical abstract Molar concentration of trans-cinnamic acid in various polar and non-polar solvents.
Bryan Charleston - One of the best experts on this subject based on the ideXlab platform.
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Identification of a Newly Conserved SLA-II Epitope in a Structural Protein of Swine Influenza Virus.
Frontiers in immunology, 2020Co-Authors: Massimiliano Baratelli, Johanneke D. Hemmink, Bryan Charleston, Sophie B. Morgan, Elizabeth Reid, Brigid Veronica Carr, Eric A. Lefevre, Sergio Montaner-tarbes, Lorenzo Fraile, Elma TchilianAbstract:Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs.
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The major histocompatibility complex homozygous inbred Babraham pig as a resource for veterinary and translational medicine.
HLA, 2018Co-Authors: John C. Schwartz, Johanneke D. Hemmink, Simon P. Graham, Elma Tchilian, Bryan Charleston, Sabine E. Hammer, John A. HammondAbstract:The Babraham pig is a highly inbred breed first developed in the United Kingdom approximately 50 years ago. Previous reports indicate a very high degree of homozygosity across the genome, including the major histocompatibility complex (MHC) region, but confirmation of homozygosity at the specific MHC loci was lacking. Using both direct sequencing and PCR‐based sequence‐specific typing, we confirm that Babraham pigs are essentially homozygous at their MHC loci and formalise their MHC haplotype as Hp‐55.6. This enhances the utility of the Babraham pig as a useful biomedical model for studies in which controlling for genetic variation is important.
Johanneke D. Hemmink - One of the best experts on this subject based on the ideXlab platform.
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Identification of a Newly Conserved SLA-II Epitope in a Structural Protein of Swine Influenza Virus.
Frontiers in immunology, 2020Co-Authors: Massimiliano Baratelli, Johanneke D. Hemmink, Bryan Charleston, Sophie B. Morgan, Elizabeth Reid, Brigid Veronica Carr, Eric A. Lefevre, Sergio Montaner-tarbes, Lorenzo Fraile, Elma TchilianAbstract:Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs.
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The major histocompatibility complex homozygous inbred Babraham pig as a resource for veterinary and translational medicine.
HLA, 2018Co-Authors: John C. Schwartz, Johanneke D. Hemmink, Simon P. Graham, Elma Tchilian, Bryan Charleston, Sabine E. Hammer, John A. HammondAbstract:The Babraham pig is a highly inbred breed first developed in the United Kingdom approximately 50 years ago. Previous reports indicate a very high degree of homozygosity across the genome, including the major histocompatibility complex (MHC) region, but confirmation of homozygosity at the specific MHC loci was lacking. Using both direct sequencing and PCR‐based sequence‐specific typing, we confirm that Babraham pigs are essentially homozygous at their MHC loci and formalise their MHC haplotype as Hp‐55.6. This enhances the utility of the Babraham pig as a useful biomedical model for studies in which controlling for genetic variation is important.
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Identification of influenza-specific T-cells in the bronchoalveolar lavage from Babraham pigs infected with pandemic H1N1 swine influenza virus.
2018Co-Authors: Katie Tungatt, Johanneke D. Hemmink, Sophie B. Morgan, Garry Dolton, Meriem Attaf, Anna Fuller, Thomas Whalley, Emily Porter, Barbara Szomolay, Maria MontoyaAbstract:Babraham pigs were either left uninfected (741) or infected intranasally with pandemic H1N1 [A/sw/Eng/1353/09] (742, 744, 745). The pigs were culled on day 0 (741), 5 (744) or 14 (742 and 745) post infection. (A) 200,000 bronchoalveolar lavage cells from pig 745 (infected, day 14 cull) were incubated alone, with 10-5M peptide, or virus for 16–18 h. A Babraham kidney cell line was included in each well (15,000 per well) to act as antigen presenting cells. All conditions were performed in duplicate and spot forming cells (SFCs) detected by IFNγ ELISPOT and displayed as mean +SEM and scaled (X5) to 106 BAL cells. (B) Irrelevant and nucleoprotein peptide-SLA-1*14:02 and SLA-2*11:04 tetramer staining was performed on thawed bronchoalveolar lavage samples and the percentage of tetramer+ cells of CD8β cells displayed in red. Nucleoprotein peptide sequences are shown. Irrelevant tetramers were SLA-1*14:02-AFAAAAAAL and SLA-2*11:04-AGAAAAAAI. Gating strategy: lymphocytes, single cells, viability (Vividneg) CD3+ CD14neg then CD8β+ CD4+ and displayed as CD8β versus tetramer (S1 Fig).
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Nuceloprotein pSLA-I tetramer staining of tissues from influenza vaccinated Babraham Pigs.
2018Co-Authors: Katie Tungatt, Johanneke D. Hemmink, Sophie B. Morgan, Garry Dolton, Meriem Attaf, Anna Fuller, Thomas Whalley, Emily Porter, Barbara Szomolay, Maria MontoyaAbstract:Babraham pig 625 (left panel of 21 plots) and 650 (right panel of 21 plots) received H5N1 S-FLU intranasally and inactivated H1N1 virus [A/Swine/Spain/SF11131/2007] with montanide adjuvant intramuscularly, followed by a boost at day 25 using the same preparation. Pigs were culled at day 38 (day 13 post boost) and blood, bronchoalveolar lavage (BAL) and tracheobronchial lymph nodes (TBLNs) harvested and frozen as single cell suspensions. Tetramer staining was performed on thawed cells from the blood, BAL and TBLN using a no tetramer control, and staining with Irrelevant and nucleoprotein peptide tetramers. The sequences for the nucleoprotein peptides are shown. Irrelevant tetramers: SLA-1*14:02-AFAAAAAAL, SLA-2*11:04-AGAAAAAAI (pig 625) and SLA-2*11:04-GAGGGGGGI (pig 650). Gating strategy: lymphocytes, single cells, viability (Vividneg) CD3+ CD14neg then CD8β+ CD4+ and displayed as CD8β versus tetramer (S1 Fig).
William E. Acree - One of the best experts on this subject based on the ideXlab platform.
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comments on classification of biphasic solvent systems according to abraham descriptors for countercurrent chromatography
Journal of Chromatography A, 2020Co-Authors: William E. Acree, Brittani Churchill, Michael H. AbrahamAbstract:Abraham model correlations reported by Marlot and coworkers for the 1-octanol/water, 1-butanol/water, ethyl acetate/water, and heptane/methanol biphasic partitioning systems are compared to previously published Abraham model correlations. The previously published correlations for the fore-mentioned partitioning systems are based on more experimental data points, and exhibit much better descriptive ability as evidenced by much smaller standard deviations/standard errors and larger squared correlation coefficients.
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The partition of organic compounds from water into the methyl isobutyl ketone extraction solvent with updated Abraham model equation
Physics and Chemistry of Liquids, 2020Co-Authors: Katherine Smart, William E. Acree, Estefania Garcia, Blessing Oloyede, Rachel Fischer, Teresa D. Golden, Michael H. AbrahamAbstract:Experimental water-to-methyl isobutyl ketone partition coefficients have been determined for 27 different organic solutes based on gas chromatographic measurements. Updated Abraham model correlatio...
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infinite dilution activity coefficients and gas to liquid partition coefficients of organic solutes dissolved in 1 sec butyl 3 methylimidazolium bis trifluoromethylsulfonyl imide and in 1 tert butyl 3 methylimidazolium bis trifluoromethylsulfonyl imi
Physics and Chemistry of Liquids, 2019Co-Authors: Fabrice Mutelet, Ellen Qian, Gary A Baker, Sudhir Ravula, Lainey Wang, William E. AcreeAbstract:ABSTRACTInverse gas chromatography was used to measure infinite dilution activity coefficients and gas-to-liquid partition coefficients for 48 organic solute probes in either 1-sec-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide or 1-tert-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide in the temperature range from 323.15 to 373.15 K. Partial molar excess enthalpies of solution were calculated from the variation of the infinite dilution activity coefficients with temperature. Abraham model correlations were also derived from the experimental partition coefficient data. The derived Abraham model correlations describe the observed partition coefficients to within 0.11 log units.
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infinite dilution activity coefficients and gas to liquid partition coefficients of organic solutes dissolved in 1 benzylpyridinium bis trifluoromethylsulfonyl imide and 1 cyclohexylmethyl 1 methylpyrrolidinium bis trifluoromethylsulfonyl imide
Journal of Solution Chemistry, 2018Co-Authors: Fabrice Mutelet, Gary A Baker, Sudhir Ravula, Delani Woods, Xin Tong, William E. AcreeAbstract:Infinite dilution activity coefficients and gas-to-ionic liquid partition coefficients were measured for a chemically diverse set of 48 or more organic solute probes dissolved in the ionic liquids 1-benzylpyridinium bis(trifluoromethylsulfonyl)imide ([BzPy][Tf2N]) and 1-cyclohexylmethyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide ([ChxmPyrr][Tf2N]) in the temperature range from 323.15 to 373.15 K using inverse gas chromatography. Selectivities and capacities for different separation problems were calculated from the measured chromatographic data. The measured partition coefficients were correlated using mathematical equations based on the Abraham general solvation parameter model. The derived Abraham model correlations back-calculated the observed partition coefficients to within 0.12 log10 units.
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Abraham model expressions for describing water-to-organic solvent and gas-to-organic solvent partition coefficients for solute transfer into anhydrous poly(ethylene glycol) dialkyl ether solvents at 298.15 K
Physics and Chemistry of Liquids, 2016Co-Authors: Erin Hart, William E. Acree, Sarah Cheeran, Grace E. Little, Hunter Singleton, Michael H. AbrahamAbstract:ABSTRACTHenry’s law constants and infinite dilution activity coefficients were compiled from the published chemical and engineering literature for gaseous solutes and organic liquids in butyl diglyme, butyl triglyme and tetraglyme. The published literature values were converted into water-to-liquid and gas-to-liquid partition coefficients using standard thermodynamic relationships. The calculated partition coefficients were correlated mathematically with the Abraham solvation parameter model. The derived Abraham model correlations can be used to predict the partitioning behaviour of additional solutes into dry, anhydrous butyl diglyme, butyl triglyme and tetraglyme.
