The Experts below are selected from a list of 222 Experts worldwide ranked by ideXlab platform

Dorine W Swinkels - One of the best experts on this subject based on the ideXlab platform.

  • shotgun immunoproteomics to identify disease associated Bacterial Antigens application to human colon cancer
    Proteomics Clinical Applications, 2007
    Co-Authors: Harold Tjalsma, Edwin Lasonder, Marie Schollerguinard, Dorine W Swinkels
    Abstract:

    Circulating antibodies reflect a mirror view of invading Antigens that are related to infection and cancer. This was recently exemplified by using serum antibodies to capture Streptococcus bovis Antigens followed by MS to generate antigen profiles that were diagnostic for colon cancer. These Bacterial antigen profiles have a high potential to aid in the immuno-diagnosis of this disease, as the magnitude of the immune response to Bacterial Antigens is, in general, superior to the immune response against tumor (self) Antigens. In this study, the identity of individual colon cancer-associated streptococcal Antigens was revealed by enrichment of these “diagnostic” Antigens by selected patient antibodies followed by high-accuracy nanoLC-MS/MS peptide identification. This showed that both the histone-like protein HlpA and the ribosomal protein Rp L7/L12 are members of the colon cancer-associated S. bovis immunome. Both Antigens also seem to belong to the group of anchorless surface proteins, like 14 additional proteins that were co-identified in S. bovis cell wall extracts. Among these were the known streptococcal anchorless surface proteins GAPDH and Enolase. Taken together, these data show that shotgun immunoproteomics, combining immunocapture in-line with LC MS/MS, is a convenient approach for the rapid identification of disease-associated Bacterial Antigens.

  • Shotgun immunoproteomics to identify disease‐associated Bacterial Antigens: Application to human colon cancer
    PROTEOMICS – Clinical Applications, 2007
    Co-Authors: Harold Tjalsma, Edwin Lasonder, Marie Schöller-guinard, Dorine W Swinkels
    Abstract:

    Circulating antibodies reflect a mirror view of invading Antigens that are related to infection and cancer. This was recently exemplified by using serum antibodies to capture Streptococcus bovis Antigens followed by MS to generate antigen profiles that were diagnostic for colon cancer. These Bacterial antigen profiles have a high potential to aid in the immuno-diagnosis of this disease, as the magnitude of the immune response to Bacterial Antigens is, in general, superior to the immune response against tumor (self) Antigens. In this study, the identity of individual colon cancer-associated streptococcal Antigens was revealed by enrichment of these “diagnostic” Antigens by selected patient antibodies followed by high-accuracy nanoLC-MS/MS peptide identification. This showed that both the histone-like protein HlpA and the ribosomal protein Rp L7/L12 are members of the colon cancer-associated S. bovis immunome. Both Antigens also seem to belong to the group of anchorless surface proteins, like 14 additional proteins that were co-identified in S. bovis cell wall extracts. Among these were the known streptococcal anchorless surface proteins GAPDH and Enolase. Taken together, these data show that shotgun immunoproteomics, combining immunocapture in-line with LC MS/MS, is a convenient approach for the rapid identification of disease-associated Bacterial Antigens.

Harold Tjalsma - One of the best experts on this subject based on the ideXlab platform.

  • shotgun immunoproteomics to identify disease associated Bacterial Antigens application to human colon cancer
    Proteomics Clinical Applications, 2007
    Co-Authors: Harold Tjalsma, Edwin Lasonder, Marie Schollerguinard, Dorine W Swinkels
    Abstract:

    Circulating antibodies reflect a mirror view of invading Antigens that are related to infection and cancer. This was recently exemplified by using serum antibodies to capture Streptococcus bovis Antigens followed by MS to generate antigen profiles that were diagnostic for colon cancer. These Bacterial antigen profiles have a high potential to aid in the immuno-diagnosis of this disease, as the magnitude of the immune response to Bacterial Antigens is, in general, superior to the immune response against tumor (self) Antigens. In this study, the identity of individual colon cancer-associated streptococcal Antigens was revealed by enrichment of these “diagnostic” Antigens by selected patient antibodies followed by high-accuracy nanoLC-MS/MS peptide identification. This showed that both the histone-like protein HlpA and the ribosomal protein Rp L7/L12 are members of the colon cancer-associated S. bovis immunome. Both Antigens also seem to belong to the group of anchorless surface proteins, like 14 additional proteins that were co-identified in S. bovis cell wall extracts. Among these were the known streptococcal anchorless surface proteins GAPDH and Enolase. Taken together, these data show that shotgun immunoproteomics, combining immunocapture in-line with LC MS/MS, is a convenient approach for the rapid identification of disease-associated Bacterial Antigens.

  • Shotgun immunoproteomics to identify disease‐associated Bacterial Antigens: Application to human colon cancer
    PROTEOMICS – Clinical Applications, 2007
    Co-Authors: Harold Tjalsma, Edwin Lasonder, Marie Schöller-guinard, Dorine W Swinkels
    Abstract:

    Circulating antibodies reflect a mirror view of invading Antigens that are related to infection and cancer. This was recently exemplified by using serum antibodies to capture Streptococcus bovis Antigens followed by MS to generate antigen profiles that were diagnostic for colon cancer. These Bacterial antigen profiles have a high potential to aid in the immuno-diagnosis of this disease, as the magnitude of the immune response to Bacterial Antigens is, in general, superior to the immune response against tumor (self) Antigens. In this study, the identity of individual colon cancer-associated streptococcal Antigens was revealed by enrichment of these “diagnostic” Antigens by selected patient antibodies followed by high-accuracy nanoLC-MS/MS peptide identification. This showed that both the histone-like protein HlpA and the ribosomal protein Rp L7/L12 are members of the colon cancer-associated S. bovis immunome. Both Antigens also seem to belong to the group of anchorless surface proteins, like 14 additional proteins that were co-identified in S. bovis cell wall extracts. Among these were the known streptococcal anchorless surface proteins GAPDH and Enolase. Taken together, these data show that shotgun immunoproteomics, combining immunocapture in-line with LC MS/MS, is a convenient approach for the rapid identification of disease-associated Bacterial Antigens.

M. P. Hazenberg - One of the best experts on this subject based on the ideXlab platform.

  • Responses of synovial fluid and peripheral blood mononuclear cells to Bacterial Antigens and autologous antigen presenting cells.
    Annals of the Rheumatic Diseases, 1993
    Co-Authors: I. S. Klasen, Marie-josé Melief, T. J. G. Swaak, A. J. Severijnen, M. P. Hazenberg
    Abstract:

    The specificity of T cells in the inflamed joints of patients with rheumatoid arthritis (RA) has been the subject of much study. Bacterial Antigens are suspect in the aetiology of rheumatic diseases. The responsiveness of the mononuclear cell fraction of peripheral blood and synovial fluid of patients with RA and of patients with rheumatic diseases other than RA to Bacterial Antigens such as cell wall fragments of the anaerobic intestinal flora, cell wall fragments of Streptococcus pyogenes, intestinal flora derived peptidoglycan polysaccharide complexes, the 65 kilodalton protein of Mycobacterium tuberculosis, and muramyldipeptide was investigated. No significant difference in response was found to all these Bacterial Antigens in the synovial fluid of patients with RA compared with the responses in patients with other rheumatic diseases. The highest responsiveness in the synovial fluid of the patients with RA was to the streptococcal cell wall fragments and to the 65 kilodalton protein. Higher responses to several Bacterial Antigens in the synovial fluid of patients with RA were found compared with peripheral blood from the same patient group. The antigen presenting cell population of the synovial fluid in patients with RA and the patients with other rheumatic diseases was found to be stimulatory for autologous peripheral blood T cells even in the absence of antigen. This suggests an important role for the synovial antigen presenting cell in the aetiology of inflammatory joint diseases.

I. S. Klasen - One of the best experts on this subject based on the ideXlab platform.

  • Detection of intestinal flora-derived Bacterial antigen complexes in splenic macrophages of rats.
    Journal of Histochemistry & Cytochemistry, 1994
    Co-Authors: J. Kool, H. De Visser, M Y Gerrits-boeye, I. S. Klasen, Marie-josé Melief, C.g. Van Helden-meeuwsen, L. M. C. Van Lieshout, J.g.h. Ruseler-van Embden, W B Van Den Berg, G M Bahr
    Abstract:

    We studied the presence of Bacterial Antigens in rat tissues. We produced a monoclonal antibody (MAb 2E9) directed against intestinal flora-derived peptidoglycan-polysaccharide complexes from human...

  • Responses of synovial fluid and peripheral blood mononuclear cells to Bacterial Antigens and autologous antigen presenting cells.
    Annals of the Rheumatic Diseases, 1993
    Co-Authors: I. S. Klasen, Marie-josé Melief, T. J. G. Swaak, A. J. Severijnen, M. P. Hazenberg
    Abstract:

    The specificity of T cells in the inflamed joints of patients with rheumatoid arthritis (RA) has been the subject of much study. Bacterial Antigens are suspect in the aetiology of rheumatic diseases. The responsiveness of the mononuclear cell fraction of peripheral blood and synovial fluid of patients with RA and of patients with rheumatic diseases other than RA to Bacterial Antigens such as cell wall fragments of the anaerobic intestinal flora, cell wall fragments of Streptococcus pyogenes, intestinal flora derived peptidoglycan polysaccharide complexes, the 65 kilodalton protein of Mycobacterium tuberculosis, and muramyldipeptide was investigated. No significant difference in response was found to all these Bacterial Antigens in the synovial fluid of patients with RA compared with the responses in patients with other rheumatic diseases. The highest responsiveness in the synovial fluid of the patients with RA was to the streptococcal cell wall fragments and to the 65 kilodalton protein. Higher responses to several Bacterial Antigens in the synovial fluid of patients with RA were found compared with peripheral blood from the same patient group. The antigen presenting cell population of the synovial fluid in patients with RA and the patients with other rheumatic diseases was found to be stimulatory for autologous peripheral blood T cells even in the absence of antigen. This suggests an important role for the synovial antigen presenting cell in the aetiology of inflammatory joint diseases.

Andre J. Ouellette - One of the best experts on this subject based on the ideXlab platform.

  • Secretion of microbicidal α-defensins by intestinal Paneth cells in response to bacteria
    Nature Immunology, 2000
    Co-Authors: Tokiyoshi Ayabe, Donald P. Satchell, Carole L. Wilson, William C. Parks, Michael E. Selsted, Andre J. Ouellette
    Abstract:

    Paneth cells in mouse small intestinal crypts secrete granules rich in microbicidal peptides when exposed to bacteria or Bacterial Antigens. The dose-dependent secretion occurs within minutes and α-defensins, or cryptdins, account for 70% of the released bactericidal peptide activity. Gram-negative bacteria, Gram-positive bacteria, lipopolysaccharide, lipoteichoic acid, lipid A and muramyl dipeptide elicit cryptdin secretion. Live fungi and protozoa, however, do not stimulate degranulation. Thus intestinal Paneth cells contribute to innate immunity by sensing bacteria and Bacterial Antigens, and discharge microbicidal peptides at effective concentrations accordingly.

  • secretion of microbicidal alpha defensins by intestinal paneth cells in response to bacteria
    Nature Immunology, 2000
    Co-Authors: Tokiyoshi Ayabe, Donald P. Satchell, Carole L. Wilson, William C. Parks, Michael E. Selsted, Andre J. Ouellette
    Abstract:

    Paneth cells in mouse small intestinal crypts secrete granules rich in microbicidal peptides when exposed to bacteria or Bacterial Antigens. The dose-dependent secretion occurs within minutes and α-defensins, or cryptdins, account for 70% of the released bactericidal peptide activity. Gram-negative bacteria, Gram-positive bacteria, lipopolysaccharide, lipoteichoic acid, lipid A and muramyl dipeptide elicit cryptdin secretion. Live fungi and protozoa, however, do not stimulate degranulation. Thus intestinal Paneth cells contribute to innate immunity by sensing bacteria and Bacterial Antigens, and discharge microbicidal peptides at effective concentrations accordingly.