The Experts below are selected from a list of 90 Experts worldwide ranked by ideXlab platform

Alan R. Davidson - One of the best experts on this subject based on the ideXlab platform.

  • The phage λ major tail protein structure reveals a common evolution for long-tailed phages and the type VI Bacterial Secretion System
    Proceedings of the National Academy of Sciences of the United States of America, 2009
    Co-Authors: Lisa G. Pell, Voula Kanelis, Logan W. Donaldson, P. Lynne Howell, Alan R. Davidson
    Abstract:

    Most bacteriophages possess long tails, which serve as the conduit for genome delivery. We report the solution structure of the N-terminal domain of gpV, the protein comprising the major portion of the noncontractile phage λ tail tube. This structure is very similar to a previously solved tail tube protein from a contractile-tailed phage, providing the first direct evidence of an evolutionary connection between these 2 distinct types of phage tails. A remarkable structural similarity is also seen to Hcp1, a component of the Bacterial type VI Secretion System. The hexameric structure of Hcp1 and its ability to form long tubes are strikingly reminiscent of gpV when it is polymerized into a tail tube. These data coupled with other similarities between phage and type VI Secretion proteins support an evolutionary relationship between these Systems. Using Hcp1 as a model, we propose a polymerization mechanism for gpV involving several disorder-to-order transitions.

Anders Sjöstedt - One of the best experts on this subject based on the ideXlab platform.

Christoph Dehio - One of the best experts on this subject based on the ideXlab platform.

  • New perspectives into Bacterial DNA transfer to human cells
    Trends in microbiology, 2012
    Co-Authors: Matxalen Llosa, Gunnar Schröder, Christoph Dehio
    Abstract:

    The type IV Secretion System (T4SS) VirB/D4 of the facultative intracellular pathogen Bartonella henselae is known to translocate Bacterial effector proteins into human cells. Two recent reports on DNA transfer into human cells have demonstrated the versatility of this Bacterial Secretion System for macromolecular substrate transfer. Moreover, these findings have opened the possibility for developing new tools for DNA delivery into specific human cell types. DNA can be introduced into these cells covalently attached to a site-specific integrase with potential target sequences in the human genome. This novel DNA delivery System is discussed in the context of existing methods for genetic modification of human cells.

Lisa G. Pell - One of the best experts on this subject based on the ideXlab platform.

  • The phage λ major tail protein structure reveals a common evolution for long-tailed phages and the type VI Bacterial Secretion System
    Proceedings of the National Academy of Sciences of the United States of America, 2009
    Co-Authors: Lisa G. Pell, Voula Kanelis, Logan W. Donaldson, P. Lynne Howell, Alan R. Davidson
    Abstract:

    Most bacteriophages possess long tails, which serve as the conduit for genome delivery. We report the solution structure of the N-terminal domain of gpV, the protein comprising the major portion of the noncontractile phage λ tail tube. This structure is very similar to a previously solved tail tube protein from a contractile-tailed phage, providing the first direct evidence of an evolutionary connection between these 2 distinct types of phage tails. A remarkable structural similarity is also seen to Hcp1, a component of the Bacterial type VI Secretion System. The hexameric structure of Hcp1 and its ability to form long tubes are strikingly reminiscent of gpV when it is polymerized into a tail tube. These data coupled with other similarities between phage and type VI Secretion proteins support an evolutionary relationship between these Systems. Using Hcp1 as a model, we propose a polymerization mechanism for gpV involving several disorder-to-order transitions.

Kun Sun - One of the best experts on this subject based on the ideXlab platform.