The Experts below are selected from a list of 27138 Experts worldwide ranked by ideXlab platform
Nathalie Parthuisot - One of the best experts on this subject based on the ideXlab platform.
-
Evaluation of ChemChrome V6 for Bacterial Viability assessment in waters.
Journal of applied microbiology, 2000Co-Authors: Nathalie Parthuisot, Philippe Catala, Karine Lemarchand, Julia Baudart, Philippe LebaronAbstract:The efficiency of ChemChrome B (CB) and ChemChrome V6 (CV6) dyes to stain viable Bacterial cells in water was compared. Both dyes are fluorogenic esters converted to free fluorescein by esterase activity. The dyes were applied to a wide variety of Bacterial species, including those poorly stained by CB, and to natural waters. Some species tested gave unacceptable low fluorescence intensities by being inefficiently or non-labelled with the CB. In contrast, CV6-stained bacteria were easily detected by both flow cytometry and solid-phase cytometry. As a consequence, higher viable cell counts were found with CV6 compared with CB in natural waters. Viable counts determined by CV6 staining were always higher than cfu counts. In constrast, respiring cell counts (CTC) were always lower than CV6 counts and, in the case of tap and mineral waters, they were lower than cfu counts.
-
Effectiveness of SYTOX Green Stain for Bacterial Viability Assessment
Applied and environmental microbiology, 1998Co-Authors: Philippe Lebaron, Philippe Catala, Nathalie ParthuisotAbstract:The effectiveness of SYTOX Green nucleic acid stain for measuring Bacterial Viability was tested on starved populations of Escherichia coli and Salmonella typhimurium. This stain underestimates the fraction of dead cells within starved populations containing cells with damaged nucleic acids or membranes. Its application to natural samples should be considered with caution.
Merja E. Penttilä - One of the best experts on this subject based on the ideXlab platform.
-
Bacterial Viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain
Applied and Environmental Microbiology, 1997Co-Authors: Bruce L Roth, Stephen T. Yue, M. L. Onnela, Marja Ilmen, Paul J. Millard, Anu Saloheimo, Martin Poot, Merja E. PenttiläAbstract:A fluorescent nucleic acid stain that does not penetrate living cells was used to assess the integrity of the plasma membranes of bacteria. SYTOX Green nucleic acid stain is an unsymmetrical cyanine dye with three positive charges that is completely excluded from live eukaryotic and prokaryotic cells. Binding of SYTOX Green stain to nucleic acids resulted in a > 500-fold enhancement in fluorescence emission (absorption and emission maxima at 502 and 523 nm, respectively), rendering bacteria with compromised plasma membranes brightly green fluorescent. SYTOX Green stain is readily excited by the 488-nm line of the argon ion laser. The fluorescence signal from membrane-compromised bacteria labeled with SYTOX Green stain was typically > 10-fold brighter than that from intact organisms. Bacterial suspensions labeled with SYTOX Green stain emitted green fluorescence in proportion to the fraction of permeabilized cells in the population, which was quantified by microscopy, fluorometry, or flow cytometry. Flow cytometric and fluorometric approaches were used to quantify the effect of beta-lactam antibiotics on the cell membrane integrity of Escherichia coli. Detection and discrimination of live and permeabilized cells labeled with SYTOX Green stain by flow cytometry were markedly improved over those by propidium iodide-based tests. These studies showed that Bacterial labeling with SYTOX Green stain is an effective alternative to conventional methods for measuring Bacterial Viability and antibiotic susceptibility.
Y. A. Diaz Fernandez - One of the best experts on this subject based on the ideXlab platform.
-
Bacterial Viability on chemically modified silicon nanowire arrays.
Journal of materials chemistry. B, 2016Co-Authors: Arturo Susarrey-arce, Alina Oknianska, Johannes G.e. Gardeniers, Rasmita Raval, Roald M. Tiggelaar, Ioritz Sorzabal-bellido, Fiona Mcbride, Alison J. Beckett, Y. A. Diaz FernandezAbstract:The global threat of antimicrobial resistance is driving an urgent need for novel antimicrobial strategies. Functional surfaces are essential to prevent spreading of infection and reduce surface contamination. In this study we have fabricated and characterized multiscale-functional nanotopographies with three levels of functionalization: (1) nanostructure topography in the form of silicon nanowires, (2) covalent chemical modification with (3-aminopropyl)triethoxysilane, and (3) incorporation of chlorhexidine digluconate. Cell Viability assays were carried out on two model microorganisms E. coli and S. aureus over these nanotopographic surfaces. Using SEM we have identified two growth modes producing distinctive multicellular structures, i.e. in plane growth for E. coli and out of plane growth for S. aureus. We have also shown that these chemically modified SiNWs arrays are effective in reducing the number of planktonic and surface-attached microorganisms.
Philippe Lebaron - One of the best experts on this subject based on the ideXlab platform.
-
Evaluation of ChemChrome V6 for Bacterial Viability assessment in waters.
Journal of applied microbiology, 2000Co-Authors: Nathalie Parthuisot, Philippe Catala, Karine Lemarchand, Julia Baudart, Philippe LebaronAbstract:The efficiency of ChemChrome B (CB) and ChemChrome V6 (CV6) dyes to stain viable Bacterial cells in water was compared. Both dyes are fluorogenic esters converted to free fluorescein by esterase activity. The dyes were applied to a wide variety of Bacterial species, including those poorly stained by CB, and to natural waters. Some species tested gave unacceptable low fluorescence intensities by being inefficiently or non-labelled with the CB. In contrast, CV6-stained bacteria were easily detected by both flow cytometry and solid-phase cytometry. As a consequence, higher viable cell counts were found with CV6 compared with CB in natural waters. Viable counts determined by CV6 staining were always higher than cfu counts. In constrast, respiring cell counts (CTC) were always lower than CV6 counts and, in the case of tap and mineral waters, they were lower than cfu counts.
-
Effectiveness of SYTOX Green Stain for Bacterial Viability Assessment
Applied and environmental microbiology, 1998Co-Authors: Philippe Lebaron, Philippe Catala, Nathalie ParthuisotAbstract:The effectiveness of SYTOX Green nucleic acid stain for measuring Bacterial Viability was tested on starved populations of Escherichia coli and Salmonella typhimurium. This stain underestimates the fraction of dead cells within starved populations containing cells with damaged nucleic acids or membranes. Its application to natural samples should be considered with caution.
Ben Zhong Tang - One of the best experts on this subject based on the ideXlab platform.
-
highly fluorescent and photostable probe for long term Bacterial Viability assay based on aggregation induced emission
Advanced Healthcare Materials, 2014Co-Authors: Engui Zhao, Chris Wai Tung Leung, Carrie Yin Kwan Chan, Yuning Hong, Ryan T K Kwok, Sijie Chen, Ben Zhong TangAbstract:Long-term tracking of Bacterial Viability is of great importance for monitoring the Viability change of bacteria under storage, evaluating disinfection efficiency, as well as for studying the pharmacokinetic and pharmacodynamic properties of antiBacterials. Most of the conventional Viability dyes, however, suffer from high toxicity and/or poor photostability, making them unsuitable for long-term studies. In this work, an aggregation-induced emission molecule, TPE-2BA, which can differentiate dead and living bacteria and serve as a highly fluorescent and photostable probe for long-term Viability assay. TPE-2BA is a cell-impermeable DNA stain that binds to the groove of double-stranded DNA. Bacteria with compromised membrane open the access for TPE-2BA to reach DNA, endowing it with strong emission. The feasibility of using TPE-2BA for screening effective bactericides is also demonstrated. Plate count experiment reveals that TPE-2BA poses negligible toxicity to bacteria, indicating that it is an excellent probe for long-term Bacterial Viability assay.
-
highly fluorescent and photostable probe for long term Bacterial Viability assay based on aggregation induced emission
Advanced Healthcare Materials, 2014Co-Authors: Engui Zhao, Chris Wai Tung Leung, Carrie Yin Kwan Chan, Yuning Hong, Ryan T K Kwok, Sijie Chen, Ben Zhong TangAbstract:Long-term tracking of Bacterial Viability is of great importance for monitoring the Viability change of bacteria under storage, evaluating disinfection efficiency, as well as for studying the pharmacokinetic and pharmacodynamic properties of antiBacterials. Most of the conventional Viability dyes, however, suffer from high toxicity and/or poor photostability, making them unsuitable for long-term studies. In this work, an aggregation-induced emission molecule, TPE-2BA, which can differentiate dead and living bacteria and serve as a highly fluorescent and photostable probe for long-term Viability assay. TPE-2BA is a cell-impermeable DNA stain that binds to the groove of double-stranded DNA. Bacteria with compromised membrane open the access for TPE-2BA to reach DNA, endowing it with strong emission. The feasibility of using TPE-2BA for screening effective bactericides is also demonstrated. Plate count experiment reveals that TPE-2BA poses negligible toxicity to bacteria, indicating that it is an excellent probe for long-term Bacterial Viability assay.
-
Highly Fluorescent and Photostable Probe for Long‐Term Bacterial Viability Assay Based on Aggregation‐Induced Emission
Advanced Healthcare Materials, 2013Co-Authors: Engui Zhao, Chris Wai Tung Leung, Carrie Yin Kwan Chan, Yuning Hong, Ryan T K Kwok, Sijie Chen, Ben Zhong TangAbstract:Long-term tracking of Bacterial Viability is of great importance for monitoring the Viability change of bacteria under storage, evaluating disinfection efficiency, as well as for studying the pharmacokinetic and pharmacodynamic properties of antiBacterials. Most of the conventional Viability dyes, however, suffer from high toxicity and/or poor photostability, making them unsuitable for long-term studies. In this work, an aggregation-induced emission molecule, TPE-2BA, which can differentiate dead and living bacteria and serve as a highly fluorescent and photostable probe for long-term Viability assay. TPE-2BA is a cell-impermeable DNA stain that binds to the groove of double-stranded DNA. Bacteria with compromised membrane open the access for TPE-2BA to reach DNA, endowing it with strong emission. The feasibility of using TPE-2BA for screening effective bactericides is also demonstrated. Plate count experiment reveals that TPE-2BA poses negligible toxicity to bacteria, indicating that it is an excellent probe for long-term Bacterial Viability assay.
