The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Fei Lu - One of the best experts on this subject based on the ideXlab platform.
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Analysis of Distributing Characteristics and Drug Resistance of Escherichia Coli in Nosocomial Infection
Journal of Nanhua University, 2020Co-Authors: Fei LuAbstract:Objective To investigate the distributions and drug resistance of Escherichia coli detected in our hospital. Methods Distributing conditions of 951 strains Escherichia coli among clinical samples isolated by routine Bacterium Culture in our hospital from Jan 2006 to Aug 2007 were studied retrospectively,drug resistances were explored by API half-automicroscan system. Results Separation rates of Escherichia coli in clinical samples such as urine,sputum,blood were higher,which were 27.2%,26.1% and 20.5% respectively.These strains were susceptible only to imipenem,meropenem and amikacin,and the rate of drug-resistance to other antibiotics were all more than 50.0%. Conclusions Escherichia coli has high resistance to a lot of antibiotics.Bacteria culturing and monitoring their resistance to antibiotics should be applied early in clinical jobs to guide reasonable drug prescription and control nosocomial infection effectively.
Xiong Long - One of the best experts on this subject based on the ideXlab platform.
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The Experimental Study of Antimicrobiologic Effect of Radiated Bone Incoporated with Lodophors
Practical Clinical Medicine, 2020Co-Authors: Xiong LongAbstract:Objective:To observe antimicrobiologic effect of radiated bone incoporated with iodophors.Methods:The bones collected from human and rabbit were rinsed with PBS and aseptic parceled,followed by be treated in supercleanlaminar flow room and stored as three groups below:Group A:the radiated allogeneic bone incorporated with iodophors;Group B:radiated allogeneic bone group;and Group C:deep frozen allogeneic bone group.They were assayed by Bacterium Culture and inhibiting bacteria in vitro.Results:There were bacterial growth in GroupsB and C.By contrast,bone grafts of Group A effectively inhibit growth of bacteria(P0.01).Conclusion:Radiated allogeneic bone incorporate with Iodophors should have good antimicrobiologic effect and it can avoid contamination.
Yan Xiang-jun - One of the best experts on this subject based on the ideXlab platform.
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Bacterial distribution and drug resistance in respiratory tract infection among geriatric patients.
Chinese Journal of Nosocomiology, 2020Co-Authors: Chen Yuyu, Yan Xiang-junAbstract:OBJECTIVE To investigate the pathogens causing respiratory tract infection among the aged people,and analyze their drug resistance to antibiotics.METHODS The sputum samples of the old patients with respiratory tract infection were collected from Jan 2006 to Nov 2007,and the drug resistance of the pathogens isolated by routine Bacterium Culture were analyzed.RESULTS Totally 3867 pathogen strains were detected among the aged people with respiratory tract infection.The detected rate was 39.3%,from them the Gram-negative bacteric were 67.9% followed by the fungi,which accounted for 29.9%and Gram-positive cocci accounted for 2.2%.For example the Gram-negtive bacilli were resistante to cefuroxime gentamicin,trimethoprim/sulfa methoxazole and ciprofloxacin,but more susceptive to imipenem and meropenem.Staphylococcus were sensitive to vancomycin,minocycline and nitrofurantoin.CONCLUSION Gram-negative bacilli are the major pathogens causing respiratory tract infection among the old patients and highly resistant to a lot of antibiotics,but the fungal infection can not be ignored.The drug resistance monitoring to antibiotics and early bacterial culturing of the sputum samples are very important.
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Main Pathogens in Urinary Tract Infections:Analysis of Distributing Characteristics and Drug Resistance
Chinese Journal of Nosocomiology, 2020Co-Authors: Yan Xiang-junAbstract:OBJECTIVE To investigate the bacterial distribution and antibiotic resistance of main pathogens from urinary tract infection (UTI) in our hospital for the guidance of rational use of antibiotics. METHODS The antibiotic resistance of clinical pathogen isolates from urinary tract infection by routine Bacterium Culture from Jan 2006 to Dec 2007 were studied retrospectively. The extended spectrum β-lactamases (ESBLs) were detected out to the Gram-negative bacilli. RESULTS There were 722 strains of pathogens in the whole 2773 urinary samples with the isolating rate 26.0%. Most of the Urinary tract infections of patients were caused by Gram-negative bacilli (69.4%),then by Gram-positive cocci (16.3%) and fungi (14.3%). The most common pathogens in urinary tract infection were Escherichia coli (47.9%),Enterococcus faecais (7.9%),E. faecium (4.0%),Proteus mirabilis (3.9%),and Pseudomonas aeruginosa (3.5%). The Gram-negative bacilli were found to be sensitive to imipenem and meropenem,but highly resistant to the most other antibiotics,while P. mirabilis was susceptive to amikacin and cephalosporins. The Gram-positive cocci were more sensitive to nitrofuvantoin and vancomycin,but highly resistant to penicillin,oxacillin and SMT. CONCLUSIONS E. coli is still the primary urinary pathogen among patients,and highly resistant to a lot of antibiotics,We should carry out cultivation,isolation,and antimicrobial susceptibility testing as soon as possible to guide reasonable clinical drug therapy.
Liu A - One of the best experts on this subject based on the ideXlab platform.
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PCR for the detection of 16S rRNA gene bacteria
Bulletin of Hunan Medical University, 1999Co-Authors: Li C, Tan D, Lu M, Wu Y, Zhou W, Liu G, Liu AAbstract:According to the high conservative region of 16S rRNA gene in bacteria, PCR primers of the broad-range bacteria, gram-positive bacteria and gram-negative bacteria were synthesized to detect 13 Bacterium species and (40) clinical specimens. All the tested Bacterium species were positive. The lowest concentration of Escherichia Coli detected by serial dilution was 4 CFU.ml-1. The positive rate of PCR (27/40) was higher than that of Bacterium Culture(18/40). The results indicate that these PCR primers possess high specificity and sensitivity in identifying 16S rRNA gene of bacteria.
Sahar Jahangiri Tazehkand - One of the best experts on this subject based on the ideXlab platform.
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In Vivo Measurement of Helicobacter pylori Infection
Methods of Molecular Biology, 2012Co-Authors: Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri TazehkandAbstract:Helicobacter pylori is a well-recognized gastroduodenal pathogen (National Institute of Health Consensus Conference, JAMA 272:65-9, 1994) and a class I carcinogen (International Agency for Research on Cancer, IARC Monograph on the Evaluation of Carcinogenic Risk to Humans 61:177-240, 1994) which successfully colonizes the harsh acidic environment of the stomach. H. pylori is the causative factor for peptic ulcer disease (PUD) and an independent risk factor for gastric adenocarcinoma development. Therefore, accurate detection of infection is crucial for devising proper eradication regimens and preventing the more severe GI complications.Detection of H. pylori in the gastric mucosa can be performed via (1) direct detection of the Bacterium; Culture, histology and polymerase chain reaction (PCR) or (2) indirect detection of its enzymatic products particularly urease as well as serum H. pylori-specific antibody responses, which can be detected by rapid urease test (RUT) and serology, respectively.The accuracy of these diagnostic tests is reported as follows: 98.1% for bacterial Culture, 98.1% for histology, 94.3% for PCR, 96.2% for RUT, and 84.9% for serology (Ni et al, J Pediatr 136(6):823-7, 2000).
