The Experts below are selected from a list of 21 Experts worldwide ranked by ideXlab platform

Jun Miyake - One of the best experts on this subject based on the ideXlab platform.

  • efficient hydrogen production using a multi layered photobioreactor and a photosynthetic Bacterium Mutant with reduced pigment
    International Journal of Hydrogen Energy, 2006
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Jun Miyake
    Abstract:

    Abstract A multi-layered photobioreactor (MLPR), where the light paths were formed by the localization of bacterial cells, was constructed for efficient hydrogen production. The performance was investigated under several conditions in order to clarify the effect of this reactor on hydrogen production. An analysis of the hydrogen production profile showed that the MPLR utilizes both the light that directly illuminates its surface and the light induced and diffused from its light paths for hydrogen production. It was also found that the hydrogen productivity in the MLPR was more than twice that in a plate-type reactor. When a photosynthetic Bacterium Mutant with reduced pigment, MTP4, was used, the maximum hydrogen production rate reached 2.0 l/m 2  h, which was 38% higher than that of a conventional plate-type reactor. The synergistic effect of the improvement in the reactor and the modification of the bacteria was brought about by the combination of the MLPR and MTP4, and resulted in an improvement in the hydrogen production.

  • enhancement of hydrogen production by a photosynthetic Bacterium Mutant with reduced pigment
    Journal of Bioscience and Bioengineering, 2002
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Masayasu Arakawa, Toshiro Hirai, Masayuki Hara, Jun Miyake
    Abstract:

    A novel Mutant MTP4 was created from the wild-type strain Rhodobacter sphaeroides RV by UV irradiation for the enhancement of hydrogen production. The amount of light absorbed by MTP4 was lower than that by the wild-type strain at any wavelengths ranging from 350 to 1000 nm. This nature enables the illumination of cells in the deeper parts of a reactor. The contents of bacteriochlorophylls and carotenoids of the chromatophores prepared from MTP4 under the conditions for hydrogen production were reduced to 41 and 49% of those from the wild-type strain RV, respectively. Analysis of the light-harvesting (LH) complexes by SDS-PAGE showed that the amounts of LH1s and reaction centers (RCs) in MTP4 were retained, whereas that of LH2s was much less than that in RV. Although MTP4 had less pigments, its growth rate was equivalent to that of RV over a wide range of light intensities. MTP4 produces hydrogen with a stable manner. Using a plate-type reactor, it produced 50% more hydrogen than RV. A novel method of pigment reduction was found to be effective for the enhancement of hydrogen production per unit reactor.

Toshihiko Kondo - One of the best experts on this subject based on the ideXlab platform.

  • efficient hydrogen production using a multi layered photobioreactor and a photosynthetic Bacterium Mutant with reduced pigment
    International Journal of Hydrogen Energy, 2006
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Jun Miyake
    Abstract:

    Abstract A multi-layered photobioreactor (MLPR), where the light paths were formed by the localization of bacterial cells, was constructed for efficient hydrogen production. The performance was investigated under several conditions in order to clarify the effect of this reactor on hydrogen production. An analysis of the hydrogen production profile showed that the MPLR utilizes both the light that directly illuminates its surface and the light induced and diffused from its light paths for hydrogen production. It was also found that the hydrogen productivity in the MLPR was more than twice that in a plate-type reactor. When a photosynthetic Bacterium Mutant with reduced pigment, MTP4, was used, the maximum hydrogen production rate reached 2.0 l/m 2  h, which was 38% higher than that of a conventional plate-type reactor. The synergistic effect of the improvement in the reactor and the modification of the bacteria was brought about by the combination of the MLPR and MTP4, and resulted in an improvement in the hydrogen production.

  • enhancement of hydrogen production by a photosynthetic Bacterium Mutant with reduced pigment
    Journal of Bioscience and Bioengineering, 2002
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Masayasu Arakawa, Toshiro Hirai, Masayuki Hara, Jun Miyake
    Abstract:

    A novel Mutant MTP4 was created from the wild-type strain Rhodobacter sphaeroides RV by UV irradiation for the enhancement of hydrogen production. The amount of light absorbed by MTP4 was lower than that by the wild-type strain at any wavelengths ranging from 350 to 1000 nm. This nature enables the illumination of cells in the deeper parts of a reactor. The contents of bacteriochlorophylls and carotenoids of the chromatophores prepared from MTP4 under the conditions for hydrogen production were reduced to 41 and 49% of those from the wild-type strain RV, respectively. Analysis of the light-harvesting (LH) complexes by SDS-PAGE showed that the amounts of LH1s and reaction centers (RCs) in MTP4 were retained, whereas that of LH2s was much less than that in RV. Although MTP4 had less pigments, its growth rate was equivalent to that of RV over a wide range of light intensities. MTP4 produces hydrogen with a stable manner. Using a plate-type reactor, it produced 50% more hydrogen than RV. A novel method of pigment reduction was found to be effective for the enhancement of hydrogen production per unit reactor.

Tatsuki Wakayama - One of the best experts on this subject based on the ideXlab platform.

  • efficient hydrogen production using a multi layered photobioreactor and a photosynthetic Bacterium Mutant with reduced pigment
    International Journal of Hydrogen Energy, 2006
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Jun Miyake
    Abstract:

    Abstract A multi-layered photobioreactor (MLPR), where the light paths were formed by the localization of bacterial cells, was constructed for efficient hydrogen production. The performance was investigated under several conditions in order to clarify the effect of this reactor on hydrogen production. An analysis of the hydrogen production profile showed that the MPLR utilizes both the light that directly illuminates its surface and the light induced and diffused from its light paths for hydrogen production. It was also found that the hydrogen productivity in the MLPR was more than twice that in a plate-type reactor. When a photosynthetic Bacterium Mutant with reduced pigment, MTP4, was used, the maximum hydrogen production rate reached 2.0 l/m 2  h, which was 38% higher than that of a conventional plate-type reactor. The synergistic effect of the improvement in the reactor and the modification of the bacteria was brought about by the combination of the MLPR and MTP4, and resulted in an improvement in the hydrogen production.

  • enhancement of hydrogen production by a photosynthetic Bacterium Mutant with reduced pigment
    Journal of Bioscience and Bioengineering, 2002
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Masayasu Arakawa, Toshiro Hirai, Masayuki Hara, Jun Miyake
    Abstract:

    A novel Mutant MTP4 was created from the wild-type strain Rhodobacter sphaeroides RV by UV irradiation for the enhancement of hydrogen production. The amount of light absorbed by MTP4 was lower than that by the wild-type strain at any wavelengths ranging from 350 to 1000 nm. This nature enables the illumination of cells in the deeper parts of a reactor. The contents of bacteriochlorophylls and carotenoids of the chromatophores prepared from MTP4 under the conditions for hydrogen production were reduced to 41 and 49% of those from the wild-type strain RV, respectively. Analysis of the light-harvesting (LH) complexes by SDS-PAGE showed that the amounts of LH1s and reaction centers (RCs) in MTP4 were retained, whereas that of LH2s was much less than that in RV. Although MTP4 had less pigments, its growth rate was equivalent to that of RV over a wide range of light intensities. MTP4 produces hydrogen with a stable manner. Using a plate-type reactor, it produced 50% more hydrogen than RV. A novel method of pigment reduction was found to be effective for the enhancement of hydrogen production per unit reactor.

Ziniu Yu - One of the best experts on this subject based on the ideXlab platform.

  • characteristics and flocculating mechanism of a novel bioflocculant hbf 3 produced by deep sea Bacterium Mutant halomonas sp v3a
    World Journal of Microbiology & Biotechnology, 2010
    Co-Authors: Jin He, Zongze Shao, Jibin Zhang, Ziniu Yu
    Abstract:

    A novel bioflocculant HBF-3 produced by deep-sea Bacterium Mutant Halomonas sp. V3a’ was investigated with regard to its flocculating characteristics and mechanism. 4.0 m g l−1 HBF-3 showed the maximum flocculating activity of 96.9% in 5.0 g l−1 Kaolin suspension containing 11.25 mM CaCl2, and that its flocculating activity was more than 90% within 5–40°C and over 80% in a wide pH range (3.0–11.0). Chemical analyses indicated that the biopolymer HBF-3 was mainly a polysaccharide, including neutral sugar residues (20.6%), uronic acid residues (7.6%), amino sugar residues (1.6%) and sulfate groups (5.3%). Fourier transform infrared (FTIR) spectrum showed the presence of carboxyl and hydroxyl groups in HBF-3 molecular. The average molecular mass of HBF-3, as determined by gel filtration chromatography (GFC), was approximately 590 kDa. Flocculation of Kaolin suspension with HBF-3 acted as a model to explore the flocculating mechanism in which bridging mediated by Ca2+ was proposed as the primary action based upon the experimental observations.

Jin Hejuan - One of the best experts on this subject based on the ideXlab platform.

  • characteristics and flocculating mechanism of a novel bioflocculant hbf 3 produced by deep sea Bacterium Mutant halomonas sp v3a
    2010
    Co-Authors: Jin Hejuan
    Abstract:

    A novel bioflocculant HBF-3 produced by deep- sea Bacterium Mutant Halomonas sp. V3a' was investigated with regard to its flocculating characteristics and mecha- nism. 4.0 m g l -1 HBF-3 showed the maximum flocculating activity of 96.9% in 5.0 g l -1 Kaolin suspension containing 11.25 mM CaCl2, and that its flocculating activity was more than 90% within 5-40C and over 80% in a wide pH range (3.0-11.0). Chemical analyses indicated that the biopolymer HBF-3 was mainly a polysaccharide, including neutral sugar residues (20.6%), uronic acid residues (7.6%), amino sugar residues (1.6%) and sulfate groups (5.3%). Fourier transform infrared (FTIR) spectrum showed the presence of carboxyl and hydroxyl groups in HBF-3 molecular. The average molecular mass of HBF-3, as determined by gel filtration chromatography (GFC), was approximately 590 kDa. Flocculation of Kaolin suspension with HBF-3 acted as a model to explore the flocculating mechanism in which bridging mediated by Ca 2? was proposed as the primary