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Bacterium Mutant

The Experts below are selected from a list of 21 Experts worldwide ranked by ideXlab platform

Jun Miyake – 1st expert on this subject based on the ideXlab platform

  • efficient hydrogen production using a multi layered photobioreactor and a photosynthetic Bacterium Mutant with reduced pigment
    International Journal of Hydrogen Energy, 2006
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Jun Miyake

    Abstract:

    Abstract A multi-layered photobioreactor (MLPR), where the light paths were formed by the localization of bacterial cells, was constructed for efficient hydrogen production. The performance was investigated under several conditions in order to clarify the effect of this reactor on hydrogen production. An analysis of the hydrogen production profile showed that the MPLR utilizes both the light that directly illuminates its surface and the light induced and diffused from its light paths for hydrogen production. It was also found that the hydrogen productivity in the MLPR was more than twice that in a plate-type reactor. When a photosynthetic Bacterium Mutant with reduced pigment, MTP4, was used, the maximum hydrogen production rate reached 2.0 l/m 2  h, which was 38% higher than that of a conventional plate-type reactor. The synergistic effect of the improvement in the reactor and the modification of the bacteria was brought about by the combination of the MLPR and MTP4, and resulted in an improvement in the hydrogen production.

  • enhancement of hydrogen production by a photosynthetic Bacterium Mutant with reduced pigment
    Journal of Bioscience and Bioengineering, 2002
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Masayasu Arakawa, Toshiro Hirai, Masayuki Hara, Jun Miyake

    Abstract:

    A novel Mutant MTP4 was created from the wild-type strain Rhodobacter sphaeroides RV by UV irradiation for the enhancement of hydrogen production. The amount of light absorbed by MTP4 was lower than that by the wild-type strain at any wavelengths ranging from 350 to 1000 nm. This nature enables the illumination of cells in the deeper parts of a reactor. The contents of bacteriochlorophylls and carotenoids of the chromatophores prepared from MTP4 under the conditions for hydrogen production were reduced to 41 and 49% of those from the wild-type strain RV, respectively. Analysis of the light-harvesting (LH) complexes by SDS-PAGE showed that the amounts of LH1s and reaction centers (RCs) in MTP4 were retained, whereas that of LH2s was much less than that in RV. Although MTP4 had less pigments, its growth rate was equivalent to that of RV over a wide range of light intensities. MTP4 produces hydrogen with a stable manner. Using a plate-type reactor, it produced 50% more hydrogen than RV. A novel method of pigment reduction was found to be effective for the enhancement of hydrogen production per unit reactor.

Toshihiko Kondo – 2nd expert on this subject based on the ideXlab platform

  • efficient hydrogen production using a multi layered photobioreactor and a photosynthetic Bacterium Mutant with reduced pigment
    International Journal of Hydrogen Energy, 2006
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Jun Miyake

    Abstract:

    Abstract A multi-layered photobioreactor (MLPR), where the light paths were formed by the localization of bacterial cells, was constructed for efficient hydrogen production. The performance was investigated under several conditions in order to clarify the effect of this reactor on hydrogen production. An analysis of the hydrogen production profile showed that the MPLR utilizes both the light that directly illuminates its surface and the light induced and diffused from its light paths for hydrogen production. It was also found that the hydrogen productivity in the MLPR was more than twice that in a plate-type reactor. When a photosynthetic Bacterium Mutant with reduced pigment, MTP4, was used, the maximum hydrogen production rate reached 2.0 l/m 2  h, which was 38% higher than that of a conventional plate-type reactor. The synergistic effect of the improvement in the reactor and the modification of the bacteria was brought about by the combination of the MLPR and MTP4, and resulted in an improvement in the hydrogen production.

  • enhancement of hydrogen production by a photosynthetic Bacterium Mutant with reduced pigment
    Journal of Bioscience and Bioengineering, 2002
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Masayasu Arakawa, Toshiro Hirai, Masayuki Hara, Jun Miyake

    Abstract:

    A novel Mutant MTP4 was created from the wild-type strain Rhodobacter sphaeroides RV by UV irradiation for the enhancement of hydrogen production. The amount of light absorbed by MTP4 was lower than that by the wild-type strain at any wavelengths ranging from 350 to 1000 nm. This nature enables the illumination of cells in the deeper parts of a reactor. The contents of bacteriochlorophylls and carotenoids of the chromatophores prepared from MTP4 under the conditions for hydrogen production were reduced to 41 and 49% of those from the wild-type strain RV, respectively. Analysis of the light-harvesting (LH) complexes by SDS-PAGE showed that the amounts of LH1s and reaction centers (RCs) in MTP4 were retained, whereas that of LH2s was much less than that in RV. Although MTP4 had less pigments, its growth rate was equivalent to that of RV over a wide range of light intensities. MTP4 produces hydrogen with a stable manner. Using a plate-type reactor, it produced 50% more hydrogen than RV. A novel method of pigment reduction was found to be effective for the enhancement of hydrogen production per unit reactor.

Tatsuki Wakayama – 3rd expert on this subject based on the ideXlab platform

  • efficient hydrogen production using a multi layered photobioreactor and a photosynthetic Bacterium Mutant with reduced pigment
    International Journal of Hydrogen Energy, 2006
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Jun Miyake

    Abstract:

    Abstract A multi-layered photobioreactor (MLPR), where the light paths were formed by the localization of bacterial cells, was constructed for efficient hydrogen production. The performance was investigated under several conditions in order to clarify the effect of this reactor on hydrogen production. An analysis of the hydrogen production profile showed that the MPLR utilizes both the light that directly illuminates its surface and the light induced and diffused from its light paths for hydrogen production. It was also found that the hydrogen productivity in the MLPR was more than twice that in a plate-type reactor. When a photosynthetic Bacterium Mutant with reduced pigment, MTP4, was used, the maximum hydrogen production rate reached 2.0 l/m 2  h, which was 38% higher than that of a conventional plate-type reactor. The synergistic effect of the improvement in the reactor and the modification of the bacteria was brought about by the combination of the MLPR and MTP4, and resulted in an improvement in the hydrogen production.

  • enhancement of hydrogen production by a photosynthetic Bacterium Mutant with reduced pigment
    Journal of Bioscience and Bioengineering, 2002
    Co-Authors: Toshihiko Kondo, Tatsuki Wakayama, Masayasu Arakawa, Toshiro Hirai, Masayuki Hara, Jun Miyake

    Abstract:

    A novel Mutant MTP4 was created from the wild-type strain Rhodobacter sphaeroides RV by UV irradiation for the enhancement of hydrogen production. The amount of light absorbed by MTP4 was lower than that by the wild-type strain at any wavelengths ranging from 350 to 1000 nm. This nature enables the illumination of cells in the deeper parts of a reactor. The contents of bacteriochlorophylls and carotenoids of the chromatophores prepared from MTP4 under the conditions for hydrogen production were reduced to 41 and 49% of those from the wild-type strain RV, respectively. Analysis of the light-harvesting (LH) complexes by SDS-PAGE showed that the amounts of LH1s and reaction centers (RCs) in MTP4 were retained, whereas that of LH2s was much less than that in RV. Although MTP4 had less pigments, its growth rate was equivalent to that of RV over a wide range of light intensities. MTP4 produces hydrogen with a stable manner. Using a plate-type reactor, it produced 50% more hydrogen than RV. A novel method of pigment reduction was found to be effective for the enhancement of hydrogen production per unit reactor.