The Experts below are selected from a list of 51063 Experts worldwide ranked by ideXlab platform
Qiang Zhu - One of the best experts on this subject based on the ideXlab platform.
-
an efficient plant regeneration and transformation system of ma Bamboo dendrocalamus latiflorus munro started from young shoot as explant
Frontiers in Plant Science, 2017Co-Authors: Changyang Cai, Mengqi Xiang, Wenjia Wang, Xiaoshan Tang, Caiping Zhu, Huibo Ren, Tengfei Yin, Li Zhang, Qiang ZhuAbstract:Genetic engineering technology has been successfully used in many plant species, but is limited in woody plants, especially in Bamboos. Ma Bamboo (Dendrocalamus latiflorus Munro) is one of the most important Bamboo species in Asia, and its genetic improvement was largely restricted by the lack of an efficient regeneration and transformation method. Here we reported a plantlet regeneration and Agrobacterium-mediated transformation protocol by using Ma Bamboo young shoots as explants. Under our optimized conditions, embryogenic calluses were successfully induced from the excised young shoots on callus induction medium (CIM) and rapidly grew on callus multiplication medium (CMM). Shoots and roots were regenerated on shoot induction medium (SIM) and root induction medium (RIM) respectively with high efficiency. An Agrobacterium-mediated genetic transformation protocol of Ma Bamboo was established, verified by PCR and GUS staining. Furthermore, the maize Lc gene under the control of the ubiquitin promoter was successfully introduced into Ma Bamboo genome and generated an anthocyanin over-accumulation phenotype. Our methods established here will facilitate the basic research as well as genetic breeding of this important Bamboo species.
-
An Efficient Plant Regeneration and Transformation System of Ma Bamboo (Dendrocalamus latiflorus Munro) Started from Young Shoot as Explant
Frontiers Media S.A., 2017Co-Authors: Changyang Cai, Mengqi Xiang, Wenjia Wang, Xiaoshan Tang, Caiping Zhu, Huibo Ren, Tengfei Yin, Li Zhang, Qiang ZhuAbstract:Genetic engineering technology has been successfully used in many plant species, but is limited in woody plants, especially in Bamboos. Ma Bamboo (Dendrocalamus latiflorus Munro) is one of the most important Bamboo species in Asia, and its genetic improvement was largely restricted by the lack of an efficient regeneration and transformation method. Here we reported a plantlet regeneration and Agrobacterium-mediated transformation protocol by using Ma Bamboo young shoots as explants. Under our optimized conditions, embryogenic calluses were successfully induced from the excised young shoots on callus induction medium and rapidly grew on callus multiplication medium. Shoots and roots were regenerated on shoot induction medium and root induction medium, respectively, with high efficiency. An Agrobacterium-mediated genetic transformation protocol of Ma Bamboo was established, verified by PCR and GUS staining. Furthermore, the maize Lc gene under the control of the ubiquitin promoter was successfully introduced into Ma Bamboo genome and generated an anthocyanin over-accumulation phenotype. Our methods established here will facilitate the basic research as well as genetic breeding of this important Bamboo species.Key achievements: A stable and high efficiency regeneration and Agrobacterium-mediated transformation protocol for Ma Bamboo from vegetative organ is established
Aryanti Evy - One of the best experts on this subject based on the ideXlab platform.
-
IDENTIFIKASI BAMBU DI SEMPADAN SUNGAI KEREMIT RESORT JOBEN TAMAN NASIONAL GUNUNG RINJANI LOMBOK
'Universitas Mataram', 2019Co-Authors: Santi, Desi Maya, Mulyaningsih Tri, Aryanti EvyAbstract:Abstrak : Sungai Keremit terletak di Joben Resort, Taman Nasional Gunung Rinjani, Lombok, yang memiliki ketinggian 661m-848m di atas permukaan laut, di sungai Keremit terdapat berbagai jenis tanaman, salah satunya adalah bambu yang tumbuh di sempadan sungai, palung, tebing dan tepi sungai. Penelitian ini bertujuan untuk mengetahui spesies bambu, kunci identifikasi, deskripsi, hubungan kekerabatan antar spesies bambu, peta distribusi bambu di sempadan sungai Keremit, Resort Joben, Taman Nasional Gunung Rinjani, Lombok. Pengambilan sampel dilakukan dengan menggunakan metode garis, mengumpulkan semua spesies bambu di sepanjang sempadan sungai Keremit yang diambil pada area 50 meter dari tepi kiri dan kanan palung sungai. Berdasarkan hasil identifikasi ditemukan 4 marga bambu, dengan 6 spesies dan 1 kultivar (cv), di sempadan sungai Keremit, Resort Joben, Taman Nasional Gunung Rinjani, Lombok, yaitu Schizostachyum jaculans, Gigantochloa atter, Gigantochloa apus, Dendrocalamus sp., Dendrocalamus asper, Bambusa vulgaris, dan 1 kultivar bambu, yaitu Bambusa vulgaris cv. Vittata.Kata kunci : Bambu, Tepi Sungai, Taman Nasional Gunung Rinjani, Lombok.Abstract : Keremit river is located in Joben Resort, Rinjani Mount National Park, Lombok, which has an altitude of 661m-848m above sea level, in the Keremit river there are various types of plants, one of which is Bamboo which grows on the edge of the trough, cliffs and river banks. This study aims to determine the species of Bamboo, identification keys, description, relationship between Bamboo species, Bamboos distribution map at the border of the Keremit river, Resort Joben, Rinjani Mount National Park, Lombok. Samples collections were conducted using the line sampling method, collecting all species of Bamboo in the border of the Keremit river were taken from the area of 50 meters from the left and right edges of the riverbed. Based on the identification results was 4 genera of Bamboos, with 6 species and 1 cultivated variety in the border of Keremit river, Joben Resort, Rinjani Mount National Park, Lombok, namely Schizostachyum jaculans, Gigantochloa atter, Gigantochloa apus, Dendrocalamus sp., Dendrocalamus asper, Bambusa vulgaris, and 1 cultivated variety of Bamboo, namely Bambusa vulgaris cv Vittata. Keywords: Bamboo, river banks, Rinjani Mount National Park, Lombok
-
Jenis-Jenis Bambu Dataran Tinggi di Sempadan Sungai Trengwilis Taman Nasional Gunung Rinjani Lombok
'Universitas PGRI Palembang', 2019Co-Authors: Fatmalasari Dewi, Mulyaningsih, Tri Mulyaningsih, Aryanti EvyAbstract:Indonesia has 176 species and 24 genera of Bamboos, around 11.5% of the total Bamboo species in the world. In Lombok, there were found 6 genera, 15 species and 8 varieties. Bamboo has an important role on the river banks, it can prevent erosion of the river cliffs. The purpose of this study was to identify the species of highland Bamboos, at the border of the Trengwilis river, Rinjani Mount National Park, Lombok. This research was conducted by descriptive explorative method, on July - April 2019. The Collecing data was used line sampling methode, with exploring the river border, 50 meters from the left and right edges of riverbed. Data collection in the field was carried out by recording 72 Bamboo characters. The results were found eight species of Bamboo which were included in five genera, e.g. Bambusa vulgaris Schard., Schizotachyum sp., Dendrocalamus sp. Dendrocalamus asper (Schult) Backer, Gigantochloa sp., Gigantochloa atter (Hassk.) Kurz., that were found at an attitude 623m-660m asl.; Gigantochloa apus (Schult.) Kurz. that grew as long as the Trengwilis riverbed at around atitude 623m-729m asl.; and Dinochloa sp. was found at an atitude 716m asl. Genus Dinochloa was a new record in West Nusa Tenggara Region
Zidni M. Irfan - One of the best experts on this subject based on the ideXlab platform.
-
“SEJUTA BAMBU UNTUK INDONESIAKU” Socio-Ecopreuneur Untuk Mensejahterakan Pengrajin Bambu Di Desa Hargomulyo, Gedangsari, Gunung Kidul, Yogyakarta dalam Momentum Masyarakat Ekonomi Asean (MEA)
'Universitas Nusantara PGRI Kediri', 2019Co-Authors: Hidayat Robist, Zidni M. IrfanAbstract:Indonesia's Bamboo potential is currently ranked third after India and China. Bamboo holds enormous opportunities and potential to be utilized in improving the economy. However, the potential of Bamboo possessed by this nation has not been utilized optimally, this material is very important because it can be classified into sustainable germplasm, with a note that the use is not excessive and the extraction is right. What happened in Indonesia, Bamboo continued to be cut down without any effort to plant and maintain so that the number of Bamboo populations continued to decline. Some areas with good potential for Bamboo include Gunungkidul, Special Region of Yogyakarta. Efforts to increase the value of Bamboo also need to be balanced with conservation efforts as an increase in Bamboo biodiversity itself. The existence of the Asean Economic Community (AEC) at the end of 2015 is a broader economic and market integration. This indicates open competition for trade in goods, services, investment, capital and workers. From these problems, we create a socio-ecopreneur with the theme "a million Bamboos for my Indonesia". This product is made from 100% Bamboo material, then innovates on Bamboo crafts to become a creative, eco-friendly, sustainable, and originally hand made the industrial product. This product is suitable for use as house accessories, household utilities, and others. This product uses traditional lamination technology (press) and Bamboo preservation. we also developed a Bamboo business management model that is beneficial for the economic resilience of local communities, especially for communities in Hargomulyo Village and at the same time beneficial to environmental biodiversity. Using a global interconnection ethnographic approach where we and the community are fully involved in the activity process starting from assessment, planning, implementation (action), until monitoring-evaluation.Indonesia's Bamboo potential is currently ranked third after India and China. Bamboo holds enormous opportunities and potential to be utilized in improving the economy. However, the potential of Bamboo possessed by this nation has not been utilized optimally, this material is very important because it can be classified into a sustainable germplasm, with a note that the use is not excessive and the extraction is right. What happened in Indonesia, Bamboo continued to be cut down without any effort to plant and maintain so that the number of Bamboo populations continued to decline. Some areas with good potential for Bamboo include Gunungkidul, Special Region of Yogyakarta. Efforts to increase the value of Bamboo also need to be balanced with conservation efforts as an increase in Bamboo biodiversity itself. The existence of the Asean Economic Community (AEC) at the end of 2015 is a broader economic and market integration. This indicates open competition for trade in goods, services, investment, capital and workers. From these problems, we create a socio-ecopreneur with the theme "a million Bamboos for my Indonesia". This product is made from 100% Bamboo material, then innovates on Bamboo crafts to become a creative, eco-friendly, sustainable, and originally hand made industrial product. This product is suitable for use as house accessories, household utilities, and others. This product uses traditional lamination technology (press) and Bamboo preservation. we also developed a Bamboo business management model that is beneficial for the economic resilience of local communities, especially for communities in Hargomulyo Village and at the same time beneficial to environmental biodiversity. Using a global interconnection ethnographic approach where we and the community are fully involved in the activity process starting from assessment, planing, implementation (action), until monitoring-evaluation
Robist - Hidayat - One of the best experts on this subject based on the ideXlab platform.
-
“SEJUTA BAMBU UNTUK INDONESIAKU” SOCIO-ECOPREUNEUR UNTUK MENSEJAHTERAKAN PENGRAJIN BAMBU DI DESA HARGOMULYO, GEDANGSARI, GUNUNG KIDUL, YOGYAKARTA DALAM MOMENTUM MASYARAKAT EKONOMI ASEAN( MEA)
'Universitas Putera Batam', 2019Co-Authors: Robist - HidayatAbstract:Indonesia's Bamboo potential is currently ranked third after India and China. Bamboo holds enormous opportunities and potential to be utilized in improving the economy. However, the potential of Bamboo possessed by this nation has not been utilized optimally, this material is very important because it can be classified into a sustainable germplasm, with a note that the use is not excessive and the extraction is right. What happened in Indonesia, Bamboo continued to be cut down without any effort to plant and maintain so that the number of Bamboo populations continued to decline. Some areas with good potential for Bamboo include Gunungkidul, Special Region of Yogyakarta. Efforts to increase the value of Bamboo also need to be balanced with conservation efforts as an increase in Bamboo biodiversity itself. The existence of the Asean Economic Community (AEC) at the end of 2015 is a broader economic and market integration. This indicates open competition for trade in goods, services, investment, capital and workers. From these problems, we create a socio-ecopreneur with the theme "a million Bamboos for my Indonesia". This product is made from 100% Bamboo material, then innovates on Bamboo crafts to become a creative, eco-friendly, sustainable, and originally hand made industrial product. This product is suitable for use as house accessories, household utilities, and others. This product uses traditional lamination technology (press) and Bamboo preservation. we also developed a Bamboo business management model that is beneficial for the economic resilience of local communities, especially for communities in Hargomulyo Village and at the same time beneficial to environmental biodiversity. Using a global interconnection ethnographic approach where we and the community are fully involved in the activity process starting from assessment, planing, implementation (action), until monitoring-evaluation
Changyang Cai - One of the best experts on this subject based on the ideXlab platform.
-
production of purple ma Bamboo dendrocalamus latiflorus munro with enhanced drought and cold stress tolerance by engineering anthocyanin biosynthesis
Planta, 2021Co-Authors: Mengqi Xiang, Wensha Ding, Wenjia Wang, Changyang Cai, Nannan Wang, Weiyuan Bai, Xiaoshan Tang, Caiping Zhu, Yushan Zheng, Zhaojun DingAbstract:Overexpression of the leaf color (Lc) gene in Ma Bamboo substantially increased the accumulation level of anthocyanin, and improved plant tolerance to cold and drought stresses, probably due to the increased antioxidant capacity. Most Bamboos, including Ma Bamboo (Dendrocalamus latiflorus Munro), are naturally evergreen and sensitive to cold and drought stresses, while it’s nearly impossible to make improvements through conventual breeding due to their long and irregular flowering habit. Moreover, few studies have reported Bamboo germplasm innovation through genetic engineering as Bamboo genetic transformation remains difficult. In this study, we have upregulated anthocyanin biosynthesis in Ma Bamboo, to generate non-green Ma Bamboo with increased abiotic stress tolerance. By overexpressing the maize Lc gene, a bHLH transcription activator involved in the anthocyanin biosynthesis in Ma Bamboo, we generated purple Bamboos with increased anthocyanin levels including cyanidin-3-O-rutinoside, peonidin 3-O-rutinoside, and an unknown cyanidin pentaglycoside derivative. The expression levels of 9 anthocyanin biosynthesis genes were up-regulated. Overexpression of the Lc gene improved the plant tolerance to cold and drought stress, probably due to increased antioxidant capacity. The levels of the cold- and drought-related phytohormone jasmonic acid in the transgenic plants were also enhanced, which may also contribute to the plant stress-tolerant phenotypes. High anthocyanin accumulation level did not affect plant growth. Transcriptomic analysis showed higher expressions of genes involved in the flavonoid pathway in Lc transgenic Bamboos compared with those in wild-type ones. The anthocyanin-rich Bamboos generated here provide an example of ornamental and multiple agronomic trait improvements by genetic engineering in this important grass species.
-
an efficient plant regeneration and transformation system of ma Bamboo dendrocalamus latiflorus munro started from young shoot as explant
Frontiers in Plant Science, 2017Co-Authors: Changyang Cai, Mengqi Xiang, Wenjia Wang, Xiaoshan Tang, Caiping Zhu, Huibo Ren, Tengfei Yin, Li Zhang, Qiang ZhuAbstract:Genetic engineering technology has been successfully used in many plant species, but is limited in woody plants, especially in Bamboos. Ma Bamboo (Dendrocalamus latiflorus Munro) is one of the most important Bamboo species in Asia, and its genetic improvement was largely restricted by the lack of an efficient regeneration and transformation method. Here we reported a plantlet regeneration and Agrobacterium-mediated transformation protocol by using Ma Bamboo young shoots as explants. Under our optimized conditions, embryogenic calluses were successfully induced from the excised young shoots on callus induction medium (CIM) and rapidly grew on callus multiplication medium (CMM). Shoots and roots were regenerated on shoot induction medium (SIM) and root induction medium (RIM) respectively with high efficiency. An Agrobacterium-mediated genetic transformation protocol of Ma Bamboo was established, verified by PCR and GUS staining. Furthermore, the maize Lc gene under the control of the ubiquitin promoter was successfully introduced into Ma Bamboo genome and generated an anthocyanin over-accumulation phenotype. Our methods established here will facilitate the basic research as well as genetic breeding of this important Bamboo species.
-
An Efficient Plant Regeneration and Transformation System of Ma Bamboo (Dendrocalamus latiflorus Munro) Started from Young Shoot as Explant
Frontiers Media S.A., 2017Co-Authors: Changyang Cai, Mengqi Xiang, Wenjia Wang, Xiaoshan Tang, Caiping Zhu, Huibo Ren, Tengfei Yin, Li Zhang, Qiang ZhuAbstract:Genetic engineering technology has been successfully used in many plant species, but is limited in woody plants, especially in Bamboos. Ma Bamboo (Dendrocalamus latiflorus Munro) is one of the most important Bamboo species in Asia, and its genetic improvement was largely restricted by the lack of an efficient regeneration and transformation method. Here we reported a plantlet regeneration and Agrobacterium-mediated transformation protocol by using Ma Bamboo young shoots as explants. Under our optimized conditions, embryogenic calluses were successfully induced from the excised young shoots on callus induction medium and rapidly grew on callus multiplication medium. Shoots and roots were regenerated on shoot induction medium and root induction medium, respectively, with high efficiency. An Agrobacterium-mediated genetic transformation protocol of Ma Bamboo was established, verified by PCR and GUS staining. Furthermore, the maize Lc gene under the control of the ubiquitin promoter was successfully introduced into Ma Bamboo genome and generated an anthocyanin over-accumulation phenotype. Our methods established here will facilitate the basic research as well as genetic breeding of this important Bamboo species.Key achievements: A stable and high efficiency regeneration and Agrobacterium-mediated transformation protocol for Ma Bamboo from vegetative organ is established
