The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
K Gurumurthi - One of the best experts on this subject based on the ideXlab platform.
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effects of type of explant and age plant growth regulators and Medium strength on somatic embryogenesis and plant regeneration in eucalyptus camaldulensis
Plant Cell Tissue and Organ Culture, 2010Co-Authors: M G Prakash, K GurumurthiAbstract:Plant regeneration was achieved through direct and indirect somatic embryogenesis in Eucalyptus camaldulensis. Callus was induced from mature zygotic embryos and from cotyledon explants collected from 10, 15, 25, and 30-day-old seedlings cultured on Murashige and Skoog (MS) Basal Medium supplemented with different concentrations of naphthaleneacetic acid (NAA). Maximum callus induction from mature zygotic embryos was obtained on MS Basal Medium containing 1 mg l−1 NAA. The frequency of callus development varied based on the age of the cotyledon explants 10-day-old explants giving highest percentage on MS Basal Medium supplemented with 1 mg l−1 NAA. Callus obtained from mature zygotic embryos gave highest frequency of somatic embryogenesis on MS Basal Medium containing 0.5 mg l−1 benzyladenine (BA) and 0.1 mg l−1 NAA. Separate age wise culture of the calli, obtained from cotyledons of different ages cultured separately, revealed high somatic embryogenic potential on callus from 10-day-old cotyledons. Direct somatic embryogenesis too was obtained from hypocotyl explants without an intervening callus phase on MS Basal Medium containing 0.5 mg l−1 BA. The effects of abscisic acid (ABA), sucrose, and different strengths of MS Medium on somatic embryo maturation and germination were also investigated. Number of mature somatic embryos increased with lower concentrations (0–1 mg l−1) of ABA while no significant differences were observed at higher concentrations (2–5 mg l−1) of ABA. Compared to Basal Medium containing lower concentrations of sucrose (1%), the MS Medium supplemented with higher levels of sucrose (4%) showed significantly lower frequency of mature somatic embryos. Basal Medium without any dilution gave the highest number of immature embryos. However, the number of mature embryos was high at higher Medium dilutions.
Pawan K Jaiwal - One of the best experts on this subject based on the ideXlab platform.
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the effect of tdz on organogenesis and somatic embryogenesis in pigeonpea cajanus cajan l millsp
Plant Science, 2003Co-Authors: Dolendro N Singh, Lingaraj Sahoo, Neera Bhalla Sarin, Pawan K JaiwalAbstract:Abstract The effect of TDZ was studied on seedlings of pigeonpea. Seedlings raised from decoated seeds on MS Basal Medium supplemented with a low concentration of TDZ (0.05–1.0 μM) induced multiple shoots, and an intermediary concentration (5.0 μM) produced clusters of leafy structures, and a higher concentration (10.0 or 20.0 μM) completely switched the regeneration pathway by inducing somatic embryos at the cotyledonary nodal region that developed into mature plants. Seedlings raised from seeds with intact seed coats, failed to produce multiple shoots or embryos under the same conditions. Multiple shoots were also induced from seeds exposed for short duration (24 h–14 days) on MS Basal Medium containing 10.0 μM TDZ and then transferred to MS Basal Medium. However, continuous exposer to TDZ is required for somatic embryogenesis. Histological examination revealed direct shoot organogenesis and somatic embryogenesis. All the shoots developed prolific roots at their Basal ends on MS Basal Medium containing 2.5 μM IBA. The well-developed plantlets were established in pots containing soil with 95% survival rate, and all the surviving plants were morphologically normal to plants raised from seeds. The present protocol is simple, rapid (the initiation of tissue cultures to transplantation of regenerants to soil completed in 8 week) with high regeneration frequency (75%) and applicable to seven genotypes.
Anrini Majumder - One of the best experts on this subject based on the ideXlab platform.
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Organogenesis and plant regeneration in Taxus wallichiana (Zucc.)
Plant Cell Reports, 2006Co-Authors: Mukul Manjari Datta, Anrini MajumderAbstract:We describe an efficient process for regeneration of Taxus wallichiana plants via shoot organogenesis from callus cultures derived from zygotic embryos. Zygotic embryos cultured on half strength Lloyd and McCown's Basal Medium supplemented with SH vitamin (½ WPMSH), 0.5 mg l^−1 6-benzyladenine (BA) in combination with 1.0–2.0 mg l^−1 2,4-dichlorophenoxyacetic acid (2,4-D) or α-Napthaleneacetic acid (NAA) produced two morphologically distinct types of calli-compact, green callus (CG) and compact, yellow (CY) callus after 4 weeks of culture. Optimum frequency (63%) of adventitious shoot bud induction was achieved in CG callus (3.0±0.67 shoot buds per gram of CG callus) when cultured on ½ WPMSH Basal Medium supplemented with 2.5 mg l^−1 BA after 4 weeks. The inclusion of 1% activated charcoal (AC) to ½ WPMSH Basal Medium (shoot elongation Medium) led to maximum shoot elongation (2.15 cms). Microshoots rooted in high frequency (40%) in MS Basal Medium in which the concentration of nitrates was reduced to one-fifth the normal concentration after 4 months of culture.
Sumita Jha - One of the best experts on this subject based on the ideXlab platform.
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morpho histological characterization and direct shoot organogenesis in two types of explants from bacopa monnieri on unsupplemented Basal Medium
Plant Cell Tissue and Organ Culture, 2017Co-Authors: Sayantika Sarkar, Sumita JhaAbstract:In the present study, high frequency regeneration has been obtained via de novo direct shoot organogenesis from leaf and internode explants in Murashige and Skoog (MS) Basal Medium without any phytohormone supplementation in Bacopa monnieri, an indigenous traditionally used medicinal herb. Leaves and internodes from different positions were excised from 4-weeks-old in vitro propagated B. monnieri plants and cultured on MS Basal Medium supplemented with 3% (w/v) sucrose and 0.75% (w/v) agar for 4 weeks. The induction of de novo shoot buds was observed at petiolar cut edges of leaf and both proximal and distal cut ends of internode explants within 10–15 days of culture. The first histological changes could be observed after 4–5 days, with meristematic activity of vascular bundles. Proliferation of epidermal cells gave rise to dome-shaped protuberances followed by shoot apical meristems formation and their vascular connections with explant tissues within 2 weeks of culture. However, a basipetal gradient of shoot regeneration from both types of explants collected along the branch axis was noticed after 4 weeks of culture. Leaf and internode explants near the Basal region exhibited significantly higher number of shoot buds and micro shoots (8.8/leaf explant and 15/internode explant). Microshoots (7–12 micro shoots/leaf or internode explants) elongated (shoot length 8–9 cm) within 8 weeks on phytohormone free MS Medium. Excised micro shoots rooted (100%) in hormone free MS Medium within two weeks of culture. Rooted plants were then acclimatized and transferred to field with 95% survival. This protocol may be used for micropropagation, genetic transformation as well as a model system for evaluation of changes associated with acquisition of competence of differentiated cells in phytohormone free Medium.
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In vitro tuberisation of Gloriosa superba L. on Basal Medium
Scientia Horticulturae, 2007Co-Authors: Seemanti Ghosh, Biswajit Ghosh, Sumita JhaAbstract:A suitable protocol for in vitro tuber production using non-dormant tubers of Gloriosa superba L. on Murashige and Skoog (MS) Medium without addition of plant growth regulators is reported in the present study. Among the different Basal media tested MS Medium was found to be suitable for induction and development of secondary tubers; one in vitro tuber per explant was obtained after 6 weeks and 3 tubers per explant after 12 weeks of culture. These tubers produced healthy green shoots that rooted on Basal Medium. Best rooting was noted in half strength (half organics and inorganics) MS Medium with one-fifth nitrates.
Jang R. Liu - One of the best experts on this subject based on the ideXlab platform.
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Plant Regeneration from Immature Zygotic Embryo-Derived Embryogenic Calluses and Cell Suspension Cultures of Catharanthus roseus
Plant Cell Tissue and Organ Culture, 2004Co-Authors: Suk Weon Kim, Pil Son Choi, Jang R. LiuAbstract:Culture conditions for plant regeneration in immature zygotic embryo-derived embryogenic cell suspension cultures of Catharanthus roseus (Madagascar periwinkle) ‘Little Bright Eye’ are described. Immature zygotic embryos formed off-white, friable calluses at a frequency of 20% on Murashige and Skoog (MS) Medium supplemented with 4.52 µM 2,4-dichlorophenoxyacetic acid (2,4-D) after 8 weeks of culture. After a second subculture using MS Basal Medium at 4-week intervals, off-white friable calluses formed a small quantity of yellowish, compact embryogenic calluses. Upon transfer to MS Basal Medium, embryogenic calluses gave rise to numerous somatic embryos. Cell suspension cultures were established with embryogenic calluses using liquid MS Medium supplemented with 4.52 µM 2,4-D. Embryogenic cell clumps from cell suspension cultures developed into plantlets at a frequency of 56.7% when plated onto MS Basal Medium. Plantlets were transplanted to potting soil and grown to maturity in a growth chamber.
