The Experts below are selected from a list of 52368 Experts worldwide ranked by ideXlab platform
Stephen F. Badylak - One of the best experts on this subject based on the ideXlab platform.
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the Basement Membrane component of biologic scaffolds derived from extracellular matrix
Tissue Engineering, 2006Co-Authors: Bryan N Brown, Janet Reing, Kristina Lindberg, Donna B. Stolz, Stephen F. BadylakAbstract:The extracellular matrix (ECM) has been successfully used as a scaffold for constructive remodeling of multiple tissues in both preclinical studies and in human clinical applications. The Basement Membrane is a specialized form of the ECM that supports and facilitates the growth of epithelial cell populations. The morphology and the molecular composition of the ECM, including the Basement Membrane, vary depending upon the organ from which the ECM is harvested and the methods by which it is processed for use as a medical device. Processing steps, such as decellularization, lyophilization, disinfection, and terminal sterilization, may affect the morphology and composition of an ECM scaffold, including, but not limited to, the integrity of a Basement Membrane complex. The present study evaluated the presence and integrity of a Basement Membrane complex in processed ECM derived from three different tissues: the urinary bladder, small intestine, and liver. Immunohistochemical determination of the presence and ...
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the Basement Membrane component of biologic scaffolds derived from extracellular matrix
Tissue Engineering, 2006Co-Authors: Bryan N Brown, Janet Reing, Kristina Lindberg, Donna B. Stolz, Stephen F. BadylakAbstract:The extracellular matrix (ECM) has been successfully used as a scaffold for constructive remodeling of multiple tissues in both preclinical studies and in human clinical applications. The Basement Membrane is a specialized form of the ECM that supports and facilitates the growth of epithelial cell populations. The morphology and the molecular composition of the ECM, including the Basement Membrane, vary depending upon the organ from which the ECM is harvested and the methods by which it is processed for use as a medical device. Processing steps, such as decellularization, lyophilization, disinfection, and terminal sterilization, may affect the morphology and composition of an ECM scaffold, including, but not limited to, the integrity of a Basement Membrane complex. The present study evaluated the presence and integrity of a Basement Membrane complex in processed ECM derived from three different tissues: the urinary bladder, small intestine, and liver. Immunohistochemical determination of the presence and ...
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The Basement Membrane Component of Biologic Scaffolds Derived from Extracellular Matrix
Tissue engineering, 2006Co-Authors: Bryan N Brown, Janet Reing, Kristina Lindberg, Donna B. Stolz, Stephen F. BadylakAbstract:The extracellular matrix (ECM) has been successfully used as a scaffold for constructive remodeling of multiple tissues in both preclinical studies and in human clinical applications. The Basement Membrane is a specialized form of the ECM that supports and facilitates the growth of epithelial cell populations. The morphology and the molecular composition of the ECM, including the Basement Membrane, vary depending upon the organ from which the ECM is harvested and the methods by which it is processed for use as a medical device. Processing steps, such as decellularization, lyophilization, disinfection, and terminal sterilization, may affect the morphology and composition of an ECM scaffold, including, but not limited to, the integrity of a Basement Membrane complex. The present study evaluated the presence and integrity of a Basement Membrane complex in processed ECM derived from three different tissues: the urinary bladder, small intestine, and liver. Immunohistochemical determination of the presence and localization of three Basement Membrane molecules, collagen IV, laminin, and collagen VII, was conducted for each ECM scaffold. Scanning electron microscopy (SEM) was used to further explore the surface ultrastructure of selected ECM scaffolds. The effect of a surface Basement Membrane presence upon the pattern of in vitro growth of two separate cell types, NIH 3T3 fibroblasts and human microvascular endothelial cells (HMEC), was also evaluated for each ECM scaffold. Results showed that the only intact Basement Membrane complex was found on the luminal surface of the ECM derived from the urinary bladder and that the Basement Membrane was an effective barrier to penetration of the scaffold by the seeded cells. We conclude that the urinary bladder ECM but not the small intestine- or liver-derived ECM contains a surface with composition and morphology consistent with that of an intact Basement Membrane complex, that the Basement Membrane complex can survive processing, and that the Basement Membrane structure can modulate in vitro cell growth patterns.
David R. Sherwood - One of the best experts on this subject based on the ideXlab platform.
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traversing the Basement Membrane in vivo a diversity of strategies
Journal of Cell Biology, 2014Co-Authors: Laura C Kelley, Elliott J. Hagedorn, Lauren L Lohmer, David R. SherwoodAbstract:The Basement Membrane is a dense, highly cross-linked, sheet-like extracellular matrix that underlies all epithelia and endothelia in multicellular animals. During development, leukocyte trafficking, and metastatic disease, cells cross the Basement Membrane to disperse and enter new tissues. Based largely on in vitro studies, cells have been thought to use proteases to dissolve and traverse this formidable obstacle. Surprisingly, recent in vivo studies have uncovered a remarkably diverse range of cellular- and tissue-level strategies beyond proteolysis that cells use to navigate through the Basement Membrane. These fascinating and unexpected mechanisms have increased our understanding of how cells cross this matrix barrier in physiological and disease settings.
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The netrin receptor DCC focuses invadopodia-driven Basement Membrane transmigration in vivo.
Journal of Cell Biology, 2013Co-Authors: Elliott J. Hagedorn, Meghan A. Morrissey, Joshua W. Ziel, Lara M. Linden, Zheng Wang, Qiuyi Chi, Sam A. Johnson, David R. SherwoodAbstract:Though critical to normal development and cancer metastasis, how cells traverse Basement Membranes is poorly understood. A central impediment has been the challenge of visualizing invasive cell interactions with Basement Membrane in vivo. By developing live-cell imaging methods to follow anchor cell (AC) invasion in Caenorhabditis elegans, we identify F-actin–based invadopodia that breach Basement Membrane. When an invadopodium penetrates Basement Membrane, it rapidly transitions into a stable invasive process that expands the breach and crosses into the vulval tissue. We find that the netrin receptor UNC-40 (DCC) specifically enriches at the site of Basement Membrane breach and that activation by UNC-6 (netrin) directs focused F-actin formation, generating the invasive protrusion and the cessation of invadopodia. Using optical highlighting of Basement Membrane components, we further demonstrate that rather than relying solely on proteolytic dissolution, the AC’s protrusion physically displaces Basement Membrane. These studies reveal an UNC-40–mediated morphogenetic transition at the cell–Basement Membrane interface that directs invading cells across Basement Membrane barriers.
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Cell invasion through Basement Membrane: The netrin receptor DCC guides the way.
Worm, 2013Co-Authors: Meghan A. Morrissey, Elliott J. Hagedorn, David R. SherwoodAbstract:Cell invasion through Basement Membrane is an essential part of normal development and physiology, and occurs during the pathological progression of human inflammatory diseases and cancer. F-actin-rich Membrane protrusions, called invadopodia, have been hypothesized to be the “drill bits” of invasive cells, mediating invasion through the dense, highly cross-linked Basement Membrane matrix. Though studied in vitro for over 30 y, invadopodia function in vivo has remained elusive. We have recently discovered that invadopodia breach Basement Membrane during anchor cell invasion in C. elegans, a genetically and visually tractable in vivo invasion event. Further, we found that the netrin receptor DCC localizes to the initial site of Basement Membrane breach and directs invasion through a single gap in the matrix. In this commentary, we examine how the dynamics and structure of AC-invadopodia compare with in vitro invadopodia and how the netrin receptor guides invasion through a single Basement Membrane breach. We end with a discussion of our surprising result that the anchor cell pushes the Basement Membrane aside, instead of completely dissolving it through proteolysis, and provide some ideas for how proteases and physical displacement may work together to ensure efficient and robust invasion.
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Basement Membrane sliding and targeted adhesion remodels tissue boundaries during uterine vulval attachment in caenorhabditis elegans
Nature Cell Biology, 2011Co-Authors: Shinji Ihara, Meghan A. Morrissey, Elliott J. Hagedorn, Qiuyi Chi, Fumio Motegi, James M Kramer, David R. SherwoodAbstract:Large gaps in Basement Membrane occur at sites of cell invasion and tissue remodelling in development and cancer. Though never followed directly in vivo, Basement Membrane dissolution or reduced synthesis have been postulated to create these gaps. Using landmark photobleaching and optical highlighting of laminin and type IV collagen, we find that a new mechanism, Basement Membrane sliding, underlies Basement Membrane gap enlargement during uterine-vulval attachment in Caenorhabditis elegans. Laser ablation and mutant analysis reveal that the invaginating vulval cells promote Basement Membrane movement. Further, an RNA interference and expression screen identifies the integrin INA-1/PAT-3 and VAB-19, homologue of the tumour suppressor Kank, as regulators of Basement Membrane opening. Both concentrate within vulval cells at the Basement Membrane gap boundary and halt expansion of the shifting Basement Membrane. Basement Membrane sliding followed by targeted adhesion represents a new mechanism for creating precise Basement Membrane breaches that can be used by cells to break down compartment boundaries.
Stephen P Mcadoo - One of the best experts on this subject based on the ideXlab platform.
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anti glomerular Basement Membrane disease
Rheumatic Diseases Clinics of North America, 2018Co-Authors: Kavita Gulati, Stephen P McadooAbstract:Anti-glomerular Basement Membrane (anti-GBM) disease is a rare autoimmune small vessel vasculitis characterized by autoreactivity to antigens in type IV collagen chains expressed in glomerular and alveolar Basement Membrane. The detection of circulating anti-GBM antibodies, which are shown to be directly pathogenic, is central to disease diagnosis. Clinically, anti-GBM disease usually presents with rapidly progressive glomerulonephritis with or without alveolar hemorrhage. Rapid diagnosis and early treatment are required to prevent mortality and to preserve renal function. Relapse in anti-GBM disease is uncommon. Variant and atypical forms of anti-GBM disease are increasingly recognised.
Jeffrey H Miner - One of the best experts on this subject based on the ideXlab platform.
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The glomerular Basement Membrane.
Experimental Cell Research, 2012Co-Authors: Jeffrey H MinerAbstract:The kidney's glomerular filtration barrier consists of two cells-podocytes and endothelial cells-and the glomerular Basement Membrane (GBM), a specialized extracellular matrix that lies between them. Like all Basement Membranes, the GBM consists mainly of laminin, type IV collagen, nidogen, and heparan sulfate proteoglycan. However, the GBM is unusually thick and contains particular members of these general protein families, including laminin-521, collagen α3α4α5(IV), and agrin. Knockout studies in mice and genetic findings in humans show that the laminin and type IV collagen components are particularly important for GBM structure and function, as laminin or collagen IV gene mutations cause filtration defects and renal disease of varying severities, depending on the nature of the mutations. These studies suggest that the GBM plays a crucial role in establishing and maintaining the glomerular filtration barrier.
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glomerular Basement Membrane composition and the filtration barrier
Pediatric Nephrology, 2011Co-Authors: Jeffrey H MinerAbstract:The glomerular Basement Membrane (GBM) is an especially thick Basement Membrane that contributes importantly to the kidney’s filtration barrier. The GBM derives from the fusion of separate podocyte and endothelial cell Basement Membranes during glomerulogenesis and consists primarily of laminin-521 (α5β2γ1), collagen α3α4α5(IV), nidogens-1 and -2, and agrin. Of these nine proteins, mutations in the genes encoding four of them (LAMB2, COL4A3, COL4A4, and COL4A5) cause glomerular disease in humans as well as in mice. Furthermore, mutation of a fifth (Lama5) gene in podocytes in mice causes proteinuria, nephrotic syndrome, and progression to renal failure. These results highlight the importance of the GBM for establishing and maintaining a properly functioning glomerular filtration barrier.
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Renal Basement Membrane components
Kidney international, 1999Co-Authors: Jeffrey H MinerAbstract:Renal Basement Membrane components. Basement Membranes are specialized extracellular matrices found throughout the body. They surround all epithelia, endothelia, peripheral nerves, muscle cells, and fat cells. They play particularly important roles in the kidney, as demonstrated by the fact that defects in renal Basement Membranes are associated with kidney malfunction. The major components of all Basement Membranes are laminin, collagen IV, entactin/nidogen, and sulfated proteoglycans. Each of these describes a family of related proteins that assemble with each other in the extracellular space to form the Basement Membrane. Over the last few years, new Basement Membrane components that are expressed in the kidney have been discovered. Here, the major components and their localization in mature and developing renal Basement Membranes are described. In addition, the phenotypes of Basement Membrane component gene mutations, both naturally occurring and experimental, are discussed, as is the aberrant deposition of Basement Membrane proteins in the extracellular matrix in several renal diseases.
Bryan N Brown - One of the best experts on this subject based on the ideXlab platform.
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the Basement Membrane component of biologic scaffolds derived from extracellular matrix
Tissue Engineering, 2006Co-Authors: Bryan N Brown, Janet Reing, Kristina Lindberg, Donna B. Stolz, Stephen F. BadylakAbstract:The extracellular matrix (ECM) has been successfully used as a scaffold for constructive remodeling of multiple tissues in both preclinical studies and in human clinical applications. The Basement Membrane is a specialized form of the ECM that supports and facilitates the growth of epithelial cell populations. The morphology and the molecular composition of the ECM, including the Basement Membrane, vary depending upon the organ from which the ECM is harvested and the methods by which it is processed for use as a medical device. Processing steps, such as decellularization, lyophilization, disinfection, and terminal sterilization, may affect the morphology and composition of an ECM scaffold, including, but not limited to, the integrity of a Basement Membrane complex. The present study evaluated the presence and integrity of a Basement Membrane complex in processed ECM derived from three different tissues: the urinary bladder, small intestine, and liver. Immunohistochemical determination of the presence and ...
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the Basement Membrane component of biologic scaffolds derived from extracellular matrix
Tissue Engineering, 2006Co-Authors: Bryan N Brown, Janet Reing, Kristina Lindberg, Donna B. Stolz, Stephen F. BadylakAbstract:The extracellular matrix (ECM) has been successfully used as a scaffold for constructive remodeling of multiple tissues in both preclinical studies and in human clinical applications. The Basement Membrane is a specialized form of the ECM that supports and facilitates the growth of epithelial cell populations. The morphology and the molecular composition of the ECM, including the Basement Membrane, vary depending upon the organ from which the ECM is harvested and the methods by which it is processed for use as a medical device. Processing steps, such as decellularization, lyophilization, disinfection, and terminal sterilization, may affect the morphology and composition of an ECM scaffold, including, but not limited to, the integrity of a Basement Membrane complex. The present study evaluated the presence and integrity of a Basement Membrane complex in processed ECM derived from three different tissues: the urinary bladder, small intestine, and liver. Immunohistochemical determination of the presence and ...
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The Basement Membrane Component of Biologic Scaffolds Derived from Extracellular Matrix
Tissue engineering, 2006Co-Authors: Bryan N Brown, Janet Reing, Kristina Lindberg, Donna B. Stolz, Stephen F. BadylakAbstract:The extracellular matrix (ECM) has been successfully used as a scaffold for constructive remodeling of multiple tissues in both preclinical studies and in human clinical applications. The Basement Membrane is a specialized form of the ECM that supports and facilitates the growth of epithelial cell populations. The morphology and the molecular composition of the ECM, including the Basement Membrane, vary depending upon the organ from which the ECM is harvested and the methods by which it is processed for use as a medical device. Processing steps, such as decellularization, lyophilization, disinfection, and terminal sterilization, may affect the morphology and composition of an ECM scaffold, including, but not limited to, the integrity of a Basement Membrane complex. The present study evaluated the presence and integrity of a Basement Membrane complex in processed ECM derived from three different tissues: the urinary bladder, small intestine, and liver. Immunohistochemical determination of the presence and localization of three Basement Membrane molecules, collagen IV, laminin, and collagen VII, was conducted for each ECM scaffold. Scanning electron microscopy (SEM) was used to further explore the surface ultrastructure of selected ECM scaffolds. The effect of a surface Basement Membrane presence upon the pattern of in vitro growth of two separate cell types, NIH 3T3 fibroblasts and human microvascular endothelial cells (HMEC), was also evaluated for each ECM scaffold. Results showed that the only intact Basement Membrane complex was found on the luminal surface of the ECM derived from the urinary bladder and that the Basement Membrane was an effective barrier to penetration of the scaffold by the seeded cells. We conclude that the urinary bladder ECM but not the small intestine- or liver-derived ECM contains a surface with composition and morphology consistent with that of an intact Basement Membrane complex, that the Basement Membrane complex can survive processing, and that the Basement Membrane structure can modulate in vitro cell growth patterns.