Bead-Beating - Explore the Science & Experts | ideXlab

Scan Science and Technology

Contact Leading Edge Experts & Companies

Bead-Beating

The Experts below are selected from a list of 7578 Experts worldwide ranked by ideXlab platform

Sophie Dubacq – 1st expert on this subject based on the ideXlab platform

  • Performing efficient sample preparation with hard tumor tissue: Precellys® Bead-Beating homogenizer solution
    Nature Methods, 2018
    Co-Authors: Sophie Dubacq

    Abstract:

    The Precellys® Evolution is the most efficient homogenizer based on Bead-Beating technology. It combines high flexibility and high efficiency for either routine or R&D projects. The instrument’s unique figure-eight multidirectional motion provides the same high level of energy and homogenization efficiency to all the tubes. The importance of sample preparation The first step of sample preparation is critical for obtaining an adequate quantity and quality of molecules of interest from biological samples. It can be a delicate, technical and time-consuming process. Bertin Technologies offers a range of robust, efficient and flexible homogenizers based on Bead-Beating

  • Performing efficient sample preparation with hard tumor tissue: Precellys[reg] Bead-Beating homogenizer solution
    Nature Methods, 2016
    Co-Authors: Sophie Dubacq

    Abstract:

    Performing efficient sample preparation with hard tumor tissue: Precellys ® Bead-Beating homogenizer solution

Jeffrey D Whyte – 2nd expert on this subject based on the ideXlab platform

  • Advances in Flavivirus research applications: new techniques using the FastPrep-24 5G^™ sample preparation system
    Nature Methods, 2016
    Co-Authors: Jeffrey D Whyte

    Abstract:

    The FastPrep-24 5G is the most innovative Bead-Beating sample-preparation instrument and produces quantitative, thorough and rapid grinding, lysis and homogenization of routine and resistant samples.

  • Advances in Flavivirus research applications: new techniques using the FastPrep-24 5G[trade] sample preparation system
    Nature Methods, 2016
    Co-Authors: Jeffrey D Whyte

    Abstract:

    The FastPrep-24 5G is the most innovative Bead-Beating sample-preparation instrument and produces quantitative, thorough and rapid grinding, lysis and homogenization of routine and resistant samples.

Maria M M Kaisar – 3rd expert on this subject based on the ideXlab platform

  • improved diagnosis of trichuris trichiura by using a bead beating procedure on ethanol preserved stool samples prior to dna isolation and the performance of multiplex real time pcr for intestinal parasites
    Parasitology, 2017
    Co-Authors: Maria M M Kaisar, Eric A T Brienen, Yenny Djuardi, Erliyani Sartono, Maria Yazdanbakhsh, Jaco J Verweij, Taniawati Supali, Lisette Van Lieshout

    Abstract:

    For the majority of intestinal parasites, real-time PCR-based diagnosis outperforms microscopy. However, the data for Trichuris trichiura have been less convincing and most comparative studies have been performed in populations with low prevalence. This study aims to improve detection of T. trichuria DNA in human stool by evaluating four sample preparation methods. Faecal samples ( n = 60) were collected at Flores island, Indonesia and examined by microscopy. Aliquots were taken and a Bead-Beating procedure was used both on directly frozen stool and on material preserved with 96% ethanol. PCR on frozen samples showed 40% to be positive for T. trichiura , compared with 45% positive by microscopy. The percentage positive increased when using ethanol preservation (45·0%), Bead-Beating (51·7%) and a combination (55·0%) and all three methods showed significantly higher DNA loads. The various procedures had a less pronounced effect on the PCR results of nine other parasite targets tested. Most prevalent were Ascaris lumbricoides (≈60%), Necator americanus (≈60%), Dientamoeba fragilis (≈50%) and Giardia lamblia (≈12%). To validate the practicality of the procedure, Bead-Beating was applied in a population-based survey testing 910 stool samples. Findings confirmed Bead-Beating before DNA extraction to be a highly efficient procedure for the detection of T. trichiura DNA in stool.