The Experts below are selected from a list of 176286 Experts worldwide ranked by ideXlab platform
Lisbeth Palm - One of the best experts on this subject based on the ideXlab platform.
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matrix assisted laser desorption ionization mass spectrometry Sample Preparation techniques designed for various peptide and protein analytes
Journal of Mass Spectrometry, 1997Co-Authors: Martin Kussmann, Eckhard Nordhoff, Henrik Rahbeknielsen, Sophie Haebel, Martin Rossellarsen, Lene Jakobsen, Johan Gobom, Ekatarina Mirgorodskaya, Anne Krollkristensen, Lisbeth PalmAbstract:This study encompasses a collection of experiences with regard to numerous matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) Sample Preparation techniques in terms of their suitability for different peptide and protein analytes. Variants of both established and new Sample Preparation techniques for the MALDI-MS analysis of peptides and proteins are described. The importance of matrix selection, matrix and analyte concentration, pH adjustment, crystallization conditions and the use of additives is evaluated. The tolerance of the different Sample Preparations towards salts, buffers, synthetic polymers, detergents, denaturants and other contaminants, and also the influence of the Preparation methods on undesired amino acid side-chain oxidation, are investigated. Moreover, the performance of on-target tryptic digestion and on-target disulfide reduction is shown and a microscale purification procedure is described. According to this study, there is no universally applicable Sample Preparation for a broad variety of analytes. Rather, it is necessary to specifically adapt the Sample Preparation to the analyte properties. © 1997 John Wiley & Sons, Ltd.
Hugo M Santos - One of the best experts on this subject based on the ideXlab platform.
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ultrasonic based filter aided Sample Preparation as the general method to Sample Preparation in proteomics
Analytical Chemistry, 2020Co-Authors: Luis B Carvalho, Jose Luis Capelomartinez, Carlos Lodeiro, Jacek R Wiśniewski, Hugo M SantosAbstract:We propose a new high-throughput ultrafast method for large-scale proteomics approaches by speeding up the classic filter aided Sample Preparation protocol, FASP, from overnight to 2.5 h. Thirty-si...
Matthias Mann - One of the best experts on this subject based on the ideXlab platform.
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universal Sample Preparation method for proteome analysis
Nature Methods, 2009Co-Authors: Jacek R Wisniewski, Nagarjuna Nagaraj, Matthias MannAbstract:We describe a method, filter-aided Sample Preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
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universal Sample Preparation method for proteome analysis
Nature Methods, 2009Co-Authors: Jacek R Wiśniewski, Nagarjuna Nagaraj, Alexandre Zougman, Matthias MannAbstract:A method, filter-aided Sample Preparation (FASP) combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics, allowing deeper proteomic coverage in a shorter analysis time, using small Sample amounts. We describe a method, filter-aided Sample Preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
Martin Kussmann - One of the best experts on this subject based on the ideXlab platform.
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matrix assisted laser desorption ionization mass spectrometry Sample Preparation techniques designed for various peptide and protein analytes
Journal of Mass Spectrometry, 1997Co-Authors: Martin Kussmann, Eckhard Nordhoff, Henrik Rahbeknielsen, Sophie Haebel, Martin Rossellarsen, Lene Jakobsen, Johan Gobom, Ekatarina Mirgorodskaya, Anne Krollkristensen, Lisbeth PalmAbstract:This study encompasses a collection of experiences with regard to numerous matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) Sample Preparation techniques in terms of their suitability for different peptide and protein analytes. Variants of both established and new Sample Preparation techniques for the MALDI-MS analysis of peptides and proteins are described. The importance of matrix selection, matrix and analyte concentration, pH adjustment, crystallization conditions and the use of additives is evaluated. The tolerance of the different Sample Preparations towards salts, buffers, synthetic polymers, detergents, denaturants and other contaminants, and also the influence of the Preparation methods on undesired amino acid side-chain oxidation, are investigated. Moreover, the performance of on-target tryptic digestion and on-target disulfide reduction is shown and a microscale purification procedure is described. According to this study, there is no universally applicable Sample Preparation for a broad variety of analytes. Rather, it is necessary to specifically adapt the Sample Preparation to the analyte properties. © 1997 John Wiley & Sons, Ltd.
Jacek R Wiśniewski - One of the best experts on this subject based on the ideXlab platform.
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ultrasonic based filter aided Sample Preparation as the general method to Sample Preparation in proteomics
Analytical Chemistry, 2020Co-Authors: Luis B Carvalho, Jose Luis Capelomartinez, Carlos Lodeiro, Jacek R Wiśniewski, Hugo M SantosAbstract:We propose a new high-throughput ultrafast method for large-scale proteomics approaches by speeding up the classic filter aided Sample Preparation protocol, FASP, from overnight to 2.5 h. Thirty-si...
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universal Sample Preparation method for proteome analysis
Nature Methods, 2009Co-Authors: Jacek R Wiśniewski, Nagarjuna Nagaraj, Alexandre Zougman, Matthias MannAbstract:A method, filter-aided Sample Preparation (FASP) combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics, allowing deeper proteomic coverage in a shorter analysis time, using small Sample amounts. We describe a method, filter-aided Sample Preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
