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Yasuhide Nakayama - One of the best experts on this subject based on the ideXlab platform.
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Long-term outcomes of patch tracheoplasty using collagenous tissue membranes (biosheets) produced by in-body tissue architecture in a Beagle model.
Surgery today, 2019Co-Authors: Satoshi Umeda, Yasuhide Nakayama, Yuichi Takama, Takeshi Terazawa, Iwai Ryosuke, Shohei Hiwatashi, Kengo Nakahata, Hiroomi OkuyamaAbstract:PURPOSE Although various artificial tracheas have been developed, none have proven satisfactory for clinical use. In-body tissue architecture (IBTA) has enabled us to produce collagenous tissues with a wide range of shapes and sizes to meet the needs of individual recipients. In the present study, we investigated the long-term outcomes of patch tracheoplasty using an IBTA-induced collagenous tissue membrane ("biosheet") in a Beagle model. METHODS Nine adult female Beagles were used. Biosheets were prepared by embedding cylindrical molds assembled with a silicone rod and a slitting pipe into dorsal subcutaneous pouches for 2 months. The sheets were then implanted by patch tracheoplasty. An endoscopic evaluation was performed after 1, 3, or 12 months. The implanted biosheets were harvested for a histological evaluation at the same time points. RESULTS All animals survived the study. At 1 month after tracheoplasty, the anastomotic parts and internal surface of the biosheets were smooth with ciliated columnar epithelium, which regenerated into the internal surface of the biosheet. The chronological spread of chondrocytes into the biosheet was observed at 3 and 12 months. CONCLUSIONS Biosheets showed excellent performance as a scaffold for trachea regeneration with complete luminal epithelium and partial chondrocytes in a 1-year Beagle implantation model of patch tracheoplasty.
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abstract 13903 application of in vivo tissue engineered autologous biotube vascular grafts prepared using a less invasive device for hemodialysis access in a canine model
Circulation, 2015Co-Authors: Maya Furukoshi, Yasuhide NakayamaAbstract:Purpose: When arteriovenous (AV) fistula is not feasible for vascular access in most patients on hemodialysis, the second choice is interposition AV fistula using synthetic graft. However, the grafts pose risks such as infection and low patency. Therefore, we created in vivo tissue-engineered “Biotube” vascular grafts formed with autologous tissue, based on the tissue encapsulation phenomenon. Previously, we confirmed that vascular-like structure was reconstructed in implanted Biotubes with year-ordered patency in vivo. In this study, we evaluated whether Biotubes could replace vascular access grafts for hemodialysis in the cervical vessels of Beagles. Methods and Results: The mold for Biotube preparation was assembled by inserting a silicone rod (external diameter 4 mm; length 50 mm) into an acrylate tubular cover (internal diameter 6 mm; length 50 mm with several longitudinal slits). The molds were embedded into the dorsal subcutaneous pouches of Beagles (n=3) for 4 weeks, after which they were harvested by a minimally invasive technique from the same incision (width ca. 20 mm). After removing the molds, Biotubes formed by tissue migration to a silicone surface through the mold slits (internal diameter 4 mm; length 45 mm; wall thickness ca. 1 mm) were obtained. Biotubes were then bypassed between the carotid artery (side-to-end anastomosis) and jugular vein (end-to-end anastomosis) of anesthetized Beagles. Following placement of Biotubes, continuous thrill was felt by palpation and ultrasound showed turbulent blood flow. After one month, angiography showed no stenosis, elongation, or hemorrhage in any Biotubes. Percutaneous puncturing with a needle, blood removal and resupply from the needle, and astirction within several minutes were feasible. Conclusions: In our Beagle model, Biotubes successfully created an AV shunt that maintained steady blood flow, suggesting that Biotubes have potential clinical use in maintaining vascular access for hemodialysis.
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Development of tissue‐engineered self‐expandable aortic stent grafts (Bio stent grafts) using in‐body tissue architecture technology in Beagles
Journal of Biomedical Materials Research Part B, 2014Co-Authors: Hidetake Kawajiri, Takeshi Moriwaki, Masashi Yamanami, Hatsue Ishibashi-ueda, Keiichi Kanda, Hitoshi Yaku, Takeshi Mizuno, Yasuhide NakayamaAbstract:In this study, we aimed to describe the development of tissue-engineered self-expandable aortic stent grafts (Bio stent graft) using in-body tissue architecture technology in Beagles and to determine its mechanical and histological properties. The preparation mold was assembled by insertion of an acryl rod (outer diameter, 8.6 mm; length, 40 mm) into a self-expanding nitinol stent (internal diameter, 9.0 mm; length, 35 mm). The molds (n = 6) were embedded into the subcutaneous pouches of three Beagles for 4 weeks. After harvesting and removing each rod, the excessive fragile tissue connected around the molds was trimmed, and thus tubular autologous connective tissues with the stent were obtained for use as Bio stent grafts (outer diameter, approximately 9.3 mm in all molds). The stent strut was completely surrounded by the dense collagenous membrane (thickness, ∼150 µm). The Bio stent graft luminal surface was extremely flat and smooth. The graft wall of the Bio stent graft possessed an elastic modulus that was almost two times higher than that of the native Beagle abdominal aorta. This Bio stent graft is expected to exhibit excellent biocompatibility after being implanted in the aorta, which may reduce the risk of type 1 endoleaks or migration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 381–386, 2015.
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Development of tissue-engineered self-expandable aortic stent grafts (Bio stent grafts) using in-body tissue architecture technology in Beagles
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2014Co-Authors: Hidetake Kawajiri, Takeshi Moriwaki, Masashi Yamanami, Hatsue Ishibashi-ueda, Keiichi Kanda, Hitoshi Yaku, Takeshi Mizuno, Yasuhide NakayamaAbstract:In this study, we aimed to describe the development of tissue-engineered self-expandable aortic stent grafts (Bio stent graft) using in-body tissue architecture technology in Beagles and to determine its mechanical and histological properties. The preparation mold was assembled by insertion of an acryl rod (outer diameter, 8.6 mm; length, 40 mm) into a self-expanding nitinol stent (internal diameter, 9.0 mm; length, 35 mm). The molds (n = 6) were embedded into the subcutaneous pouches of three Beagles for 4 weeks. After harvesting and removing each rod, the excessive fragile tissue connected around the molds was trimmed, and thus tubular autologous connective tissues with the stent were obtained for use as Bio stent grafts (outer diameter, approximately 9.3 mm in all molds). The stent strut was completely surrounded by the dense collagenous membrane (thickness, ∼150 µm). The Bio stent graft luminal surface was extremely flat and smooth. The graft wall of the Bio stent graft possessed an elastic modulus that was almost two times higher than that of the native Beagle abdominal aorta. This Bio stent graft is expected to exhibit excellent biocompatibility after being implanted in the aorta, which may reduce the risk of type 1 endoleaks or migration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 381–386, 2015.
Hidetake Kawajiri - One of the best experts on this subject based on the ideXlab platform.
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Development of tissue‐engineered self‐expandable aortic stent grafts (Bio stent grafts) using in‐body tissue architecture technology in Beagles
Journal of Biomedical Materials Research Part B, 2014Co-Authors: Hidetake Kawajiri, Takeshi Moriwaki, Masashi Yamanami, Hatsue Ishibashi-ueda, Keiichi Kanda, Hitoshi Yaku, Takeshi Mizuno, Yasuhide NakayamaAbstract:In this study, we aimed to describe the development of tissue-engineered self-expandable aortic stent grafts (Bio stent graft) using in-body tissue architecture technology in Beagles and to determine its mechanical and histological properties. The preparation mold was assembled by insertion of an acryl rod (outer diameter, 8.6 mm; length, 40 mm) into a self-expanding nitinol stent (internal diameter, 9.0 mm; length, 35 mm). The molds (n = 6) were embedded into the subcutaneous pouches of three Beagles for 4 weeks. After harvesting and removing each rod, the excessive fragile tissue connected around the molds was trimmed, and thus tubular autologous connective tissues with the stent were obtained for use as Bio stent grafts (outer diameter, approximately 9.3 mm in all molds). The stent strut was completely surrounded by the dense collagenous membrane (thickness, ∼150 µm). The Bio stent graft luminal surface was extremely flat and smooth. The graft wall of the Bio stent graft possessed an elastic modulus that was almost two times higher than that of the native Beagle abdominal aorta. This Bio stent graft is expected to exhibit excellent biocompatibility after being implanted in the aorta, which may reduce the risk of type 1 endoleaks or migration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 381–386, 2015.
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Development of tissue-engineered self-expandable aortic stent grafts (Bio stent grafts) using in-body tissue architecture technology in Beagles
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2014Co-Authors: Hidetake Kawajiri, Takeshi Moriwaki, Masashi Yamanami, Hatsue Ishibashi-ueda, Keiichi Kanda, Hitoshi Yaku, Takeshi Mizuno, Yasuhide NakayamaAbstract:In this study, we aimed to describe the development of tissue-engineered self-expandable aortic stent grafts (Bio stent graft) using in-body tissue architecture technology in Beagles and to determine its mechanical and histological properties. The preparation mold was assembled by insertion of an acryl rod (outer diameter, 8.6 mm; length, 40 mm) into a self-expanding nitinol stent (internal diameter, 9.0 mm; length, 35 mm). The molds (n = 6) were embedded into the subcutaneous pouches of three Beagles for 4 weeks. After harvesting and removing each rod, the excessive fragile tissue connected around the molds was trimmed, and thus tubular autologous connective tissues with the stent were obtained for use as Bio stent grafts (outer diameter, approximately 9.3 mm in all molds). The stent strut was completely surrounded by the dense collagenous membrane (thickness, ∼150 µm). The Bio stent graft luminal surface was extremely flat and smooth. The graft wall of the Bio stent graft possessed an elastic modulus that was almost two times higher than that of the native Beagle abdominal aorta. This Bio stent graft is expected to exhibit excellent biocompatibility after being implanted in the aorta, which may reduce the risk of type 1 endoleaks or migration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 381–386, 2015.
Lígia Miranda Ferreira Borges - One of the best experts on this subject based on the ideXlab platform.
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quantification of brown dog tick repellents 2 hexanone and benzaldehyde and release from tick resistant Beagles canis lupus familiaris
Journal of Chromatography B, 2016Co-Authors: Jaires Gomes De Oliveira Filho, Andre Lucio Franceschini Sarria, Lorena Lopes Ferreira, John C. Caulfield, Adalberto Perez A De Leon, Stephen J Powers, J. A. Pickett, Michael Alexander Birkett, Lígia Miranda Ferreira BorgesAbstract:We have recently shown that repellency of the tick Rhipicephalus sanguineus sensu lato by the tick resistant dog breed, the Beagle, is mediated by volatile organic compounds (VOCs) 2-hexanone and benzaldehyde present in Beagle odour. Ectoparasite location of animal hosts is affected by variation in these odour components and their ratios. The aim of this study was to quantify the release rate, and the ratio, of 2-hexanone and benzaldehyde from Beagles. The odour of three Beagles was collected, for four days, over one week (day 0, day 1, day 4 and day 7). The compounds were identified using coupled high-resolution gas chromatography–mass spectrometry (GC–MS), and authentic standards of compounds were used to generate external calibration curves for quantification. Both compounds were found in all dogs on all days. The amount of benzaldehyde was always higher than that of 2-hexanone and so their ratio varied from unity, on average (over time) being 3.128 ± 0.365, 1.902 ± 0.390, 1.670 ± 0.671 ng mL−1 for Beagle 1, 2 and 3, respectively. There was no significant (p < 0.05, F-test) effect of time. The overall mean was 2.233 ± 0.387 ng mL−1. These results further previous findings by documenting the presence of 2-hexanone and benzaldehyde in Beagle odour samples covering a 7-day period. This knowledge enables development of repellents to protect dogs from R. sanguineus s. l. infestation.
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identification of non host semiochemicals for the brown dog tick rhipicephalus sanguineus sensu lato acari ixodidae from tick resistant Beagles canis lupus familiaris
Ticks and Tick-borne Diseases, 2015Co-Authors: Lígia Miranda Ferreira Borges, Jaires Gomes De Oliveira Filho, Lorena Lopes Ferreira, J. A. Pickett, Carla Cristina Braz Louly, Michael Alexander BirkettAbstract:Studies have shown that the brown dog tick, Rhipicephalus sanguineus sensu lato, when fed on the Beagle breed of dog, Canis lupus familiaris, development negatively affected in comparison with tick development after feeding on the English cocker spaniel breed. Thus leading to the suggestion that Beagle dogs are be tick-resistant dogs. Behavioural studies have demonstrated that more ticks are attracted by extracts from cocker spaniels than from Beagles and that the odour of Beagles is a repellent. To test the hypothesis that resistant hosts produce repellent compounds, we undertook comparative chemical analysis on Beagle odour and cocker spaniel extracts using coupled high-resolution gas chromatography–mass spectrometry (GC–MS) and also used Petri-dish and olfactometer behavioural assays to assess the response of ticks to identified non-host compounds. The Beagle odour extracts contained almost three times as many chemical compounds as cocker spaniel samples. Several non-host compounds were identified, i.e. 2-hexanone, benzaldehyde, nonane, decane and undecane. In Petri-dish assays, 2-hexanone was repellent at 30 min at concentrations of 0.200 and 0.050 mg cm−2, whilst at 10 min, the 0.100 mg cm−2 concentration was repellent. Benzaldehyde repelled ticks at 30 min (0.200 mg cm−2) and at 5 min (0.050 mg cm−2). Undecane was repellent for R. sanguineus s.l. ticks for the first 5 min at the highest concentration tested. Nonane and decane did not show any significant repellency at any concentration or time evaluated. When 2-hexanone and benzaldehyde were combined, an increase in the repellency rate was observed, with activity comparable or better than N,N-diethyl-3-methylbenzamide (DEET). In olfactometer bioassays, a 1:1 mixture of 2-hexanone:benzaldehyde and DEET were repellent for R. sanguineus s.l. adults at the concentration of 0.200 mg cm−2. This study identified non-host semiochemicals that mediate avoidance of the Beagle dog breed by R. sanguineus s.l. This finding may enable development of new approaches to control this tick.
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Differences in the behavior of Rhipicephalus sanguineus tested against resistant and susceptible dogs
Experimental and Applied Acarology, 2010Co-Authors: Carla Cristina Braz Louly, Sara Fernandes Soares, Diana Nóbrega Silveira, Marcelo Sales Guimarães, Lígia Miranda Ferreira BorgesAbstract:To ascertain whether brown dog tick Rhipicephalus sp. infests resistant (Beagle) and susceptible (English cocker spaniel) dogs differently, five animals of each breed were maintained in a kennel whose walls were infested with 7,000 larvae, 320 nymphs, 80 males and 80 females, in 3 infestations, at 10-day intervals. Five times more ticks were found on cocker spaniels (498) than on Beagles (96). Substances were collected by rubbing pieces of clean flannel on the dogs for 15 min and these were tested for arrestment and attractiveness of ticks. Three choices were offered: cocker extract vs. control; Beagle extract vs. control, and cocker extract vs. Beagle extract. When allowed to choose between substances rubbed from dogs and a control, more ticks were arrested by extracts from the cockers than from Beagles. In the arrestment tests with only a choice between substances from dogs of each breed, more ticks were arrested by cocker substances. To test for attraction, capsules containing adsorbent were used and the tests were carried out in a Y-olfactometer. Fifteen males and 15 females were tested, for each treatment. In the olfactometer, the ticks were not attracted to the odor of either breed, however the odor of the Beagle was apparently repellent. These results indicate that R. sanguineus can use substances from the dogs to differentiate susceptible English Cocker Spaniels from resistant Beagles.
Hitoshi Yaku - One of the best experts on this subject based on the ideXlab platform.
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Development of tissue‐engineered self‐expandable aortic stent grafts (Bio stent grafts) using in‐body tissue architecture technology in Beagles
Journal of Biomedical Materials Research Part B, 2014Co-Authors: Hidetake Kawajiri, Takeshi Moriwaki, Masashi Yamanami, Hatsue Ishibashi-ueda, Keiichi Kanda, Hitoshi Yaku, Takeshi Mizuno, Yasuhide NakayamaAbstract:In this study, we aimed to describe the development of tissue-engineered self-expandable aortic stent grafts (Bio stent graft) using in-body tissue architecture technology in Beagles and to determine its mechanical and histological properties. The preparation mold was assembled by insertion of an acryl rod (outer diameter, 8.6 mm; length, 40 mm) into a self-expanding nitinol stent (internal diameter, 9.0 mm; length, 35 mm). The molds (n = 6) were embedded into the subcutaneous pouches of three Beagles for 4 weeks. After harvesting and removing each rod, the excessive fragile tissue connected around the molds was trimmed, and thus tubular autologous connective tissues with the stent were obtained for use as Bio stent grafts (outer diameter, approximately 9.3 mm in all molds). The stent strut was completely surrounded by the dense collagenous membrane (thickness, ∼150 µm). The Bio stent graft luminal surface was extremely flat and smooth. The graft wall of the Bio stent graft possessed an elastic modulus that was almost two times higher than that of the native Beagle abdominal aorta. This Bio stent graft is expected to exhibit excellent biocompatibility after being implanted in the aorta, which may reduce the risk of type 1 endoleaks or migration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 381–386, 2015.
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Development of tissue-engineered self-expandable aortic stent grafts (Bio stent grafts) using in-body tissue architecture technology in Beagles
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2014Co-Authors: Hidetake Kawajiri, Takeshi Moriwaki, Masashi Yamanami, Hatsue Ishibashi-ueda, Keiichi Kanda, Hitoshi Yaku, Takeshi Mizuno, Yasuhide NakayamaAbstract:In this study, we aimed to describe the development of tissue-engineered self-expandable aortic stent grafts (Bio stent graft) using in-body tissue architecture technology in Beagles and to determine its mechanical and histological properties. The preparation mold was assembled by insertion of an acryl rod (outer diameter, 8.6 mm; length, 40 mm) into a self-expanding nitinol stent (internal diameter, 9.0 mm; length, 35 mm). The molds (n = 6) were embedded into the subcutaneous pouches of three Beagles for 4 weeks. After harvesting and removing each rod, the excessive fragile tissue connected around the molds was trimmed, and thus tubular autologous connective tissues with the stent were obtained for use as Bio stent grafts (outer diameter, approximately 9.3 mm in all molds). The stent strut was completely surrounded by the dense collagenous membrane (thickness, ∼150 µm). The Bio stent graft luminal surface was extremely flat and smooth. The graft wall of the Bio stent graft possessed an elastic modulus that was almost two times higher than that of the native Beagle abdominal aorta. This Bio stent graft is expected to exhibit excellent biocompatibility after being implanted in the aorta, which may reduce the risk of type 1 endoleaks or migration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 381–386, 2015.
Keiichi Kanda - One of the best experts on this subject based on the ideXlab platform.
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Development of tissue‐engineered self‐expandable aortic stent grafts (Bio stent grafts) using in‐body tissue architecture technology in Beagles
Journal of Biomedical Materials Research Part B, 2014Co-Authors: Hidetake Kawajiri, Takeshi Moriwaki, Masashi Yamanami, Hatsue Ishibashi-ueda, Keiichi Kanda, Hitoshi Yaku, Takeshi Mizuno, Yasuhide NakayamaAbstract:In this study, we aimed to describe the development of tissue-engineered self-expandable aortic stent grafts (Bio stent graft) using in-body tissue architecture technology in Beagles and to determine its mechanical and histological properties. The preparation mold was assembled by insertion of an acryl rod (outer diameter, 8.6 mm; length, 40 mm) into a self-expanding nitinol stent (internal diameter, 9.0 mm; length, 35 mm). The molds (n = 6) were embedded into the subcutaneous pouches of three Beagles for 4 weeks. After harvesting and removing each rod, the excessive fragile tissue connected around the molds was trimmed, and thus tubular autologous connective tissues with the stent were obtained for use as Bio stent grafts (outer diameter, approximately 9.3 mm in all molds). The stent strut was completely surrounded by the dense collagenous membrane (thickness, ∼150 µm). The Bio stent graft luminal surface was extremely flat and smooth. The graft wall of the Bio stent graft possessed an elastic modulus that was almost two times higher than that of the native Beagle abdominal aorta. This Bio stent graft is expected to exhibit excellent biocompatibility after being implanted in the aorta, which may reduce the risk of type 1 endoleaks or migration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 381–386, 2015.
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Development of tissue-engineered self-expandable aortic stent grafts (Bio stent grafts) using in-body tissue architecture technology in Beagles
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2014Co-Authors: Hidetake Kawajiri, Takeshi Moriwaki, Masashi Yamanami, Hatsue Ishibashi-ueda, Keiichi Kanda, Hitoshi Yaku, Takeshi Mizuno, Yasuhide NakayamaAbstract:In this study, we aimed to describe the development of tissue-engineered self-expandable aortic stent grafts (Bio stent graft) using in-body tissue architecture technology in Beagles and to determine its mechanical and histological properties. The preparation mold was assembled by insertion of an acryl rod (outer diameter, 8.6 mm; length, 40 mm) into a self-expanding nitinol stent (internal diameter, 9.0 mm; length, 35 mm). The molds (n = 6) were embedded into the subcutaneous pouches of three Beagles for 4 weeks. After harvesting and removing each rod, the excessive fragile tissue connected around the molds was trimmed, and thus tubular autologous connective tissues with the stent were obtained for use as Bio stent grafts (outer diameter, approximately 9.3 mm in all molds). The stent strut was completely surrounded by the dense collagenous membrane (thickness, ∼150 µm). The Bio stent graft luminal surface was extremely flat and smooth. The graft wall of the Bio stent graft possessed an elastic modulus that was almost two times higher than that of the native Beagle abdominal aorta. This Bio stent graft is expected to exhibit excellent biocompatibility after being implanted in the aorta, which may reduce the risk of type 1 endoleaks or migration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 381–386, 2015.