The Experts below are selected from a list of 558 Experts worldwide ranked by ideXlab platform
Hanspeter M Blaschek - One of the best experts on this subject based on the ideXlab platform.
-
gene transcription repression in clostridium beijerinckii using crispr dcas9
Biotechnology and Bioengineering, 2016Co-Authors: Yi Wang, Zhong Tian Zhang, Seung Oh Seo, Patrick Lynn, Yong Su Jin, Hanspeter M BlaschekAbstract:CRISPR-Cas9 has been explored as a powerful tool for genome engineering for many organisms. Meanwhile, dCas9 which lacks endonuclease activity but can still bind to target loci has been engineered for efficient gene transcription repression. Clostridium beijerinckii, an industrially significant species capable of biosolvent production, is generally difficult to metabolically engineer. Recently, we reported our work in developing customized CRISPR-Cas9 system for genome engineering in C. beijerinckii. However, in many cases, gene expression repression (rather than actual DNA mutation) is more desirable for various biotechnological applications. Here, we further demonstrated gene transcription repression in C. beijerinckii using CRISPR-dCas9. A small RNA promoter was employed to drive the expression of the single chimeric guide RNA targeting on the promoter region of amylase gene, while a constitutive thiolase promoter was used to drive Streptococcus pyogenes dCas9 expression. The growth assay on starch agar plates showed qualitatively significant repression of amylase activity in C. beijerinckii transformant with CRISPR-dCas9 compared to the control strain. Further amylase activity quantification demonstrated consistent repression (65-97% through the fermentation process) on the activity in the transformant with CRISPR-dCas9 versus in the control. Our results provided essential references for engineering CRISPR-dCas9 as an effective tool for tunable gene transcription repression in diverse microorganisms. Biotechnol. Bioeng. 2016;113: 2739-2743. © 2016 Wiley Periodicals, Inc.
-
development of a gene knockout system using mobile group ii introns targetron and genetic disruption of acid production pathways in clostridium beijerinckii
Applied and Environmental Microbiology, 2013Co-Authors: Yi Wang, Caroline B Milne, Holger Janssen, Weiyin Lin, Gloria Phan, Yong Su Jin, Nathan D Price, Hanspeter M BlaschekAbstract:Clostridium beijerinckii is a well-known solvent-producing microorganism with great potential for biofuel and biochemical production. To better understand and improve the biochemical pathway to solvents, the development of genetic tools for engineering C. beijerinckii is highly desired. Based on mobile group II intron technology, a targetron gene knockout system was developed for C. beijerinckii in this study. This system was successfully employed to disrupt acid production pathways in C. beijerinckii, leading to pta (encoding phosphotransacetylase)- and buk (encoding butyrate kinase)-negative mutants. In addition to experimental characterization, the mutant phenotypes were analyzed in the context of our C. beijerinckii genome-scale model. Compared to those of the parental strain (C. beijerinckii 8052), acetate production in the pta mutant was substantially reduced and butyrate production was remarkably increased, while solvent production was dependent on the growth medium. The pta mutant also produced much higher levels of lactate, suggesting that disrupting pta influenced the energy generation and electron flow pathways. In contrast, acetate and butyrate production in the buk mutant was generally similar to that of the wild type, but solvent production was consistently 20 to 30% higher and glucose consumption was more rapid and complete. Our results suggest that the acid and solvent production of C. beijerinckii can be effectively altered by disrupting the acid production pathways. As the gene disruption method developed in this study does not leave any antibiotic marker in a disrupted allele, multiple and high-throughput gene disruption is feasible for elucidating genotype and phenotype relationships in C. beijerinckii.
-
butanol production from agricultural residues impact of degradation products on clostridium beijerinckii growth and butanol fermentation
Biotechnology and Bioengineering, 2007Co-Authors: Thaddeus Chukwuemeka Ezeji, Nasib Qureshi, Hanspeter M BlaschekAbstract:During pretreatment and hydrolysis of fiber-rich agricultural biomass, compounds such as salts, furfural, hydroxymethyl furfural (HMF), acetic, ferulic, glucuronic, ρ-coumaric acids, and phenolic compounds are produced. Clostridium beijerinckii BA101 can utilize the individual sugars present in lignocellulosic [e.g., corn fiber, distillers dry grain solubles (DDGS), etc] hydrolysates such as cellobiose, glucose, mannose, arabinose, and xylose. In these studies we investigated the effect of some of the lignocellulosic hydrolysate inhibitors associated with C. beijerinckii BA101 growth and acetone–butanol–ethanol (ABE) production. When 0.3 g/L ρ-coumaric and ferulic acids were introduced into the fermentation medium, growth and ABE production by C. beijerinckii BA101 decreased significantly. Furfural and HMF are not inhibitory to C. beijerinckii BA101; rather they have stimulatory effect on the growth of the microorganism and ABE production. Biotechnol. Bioeng. 2007;97:1460–1469. © 2007 Wiley Periodicals, Inc.
-
butanol production from agricultural residues impact of degradation products on clostridium beijerinckii growth and butanol fermentation
Biotechnology and Bioengineering, 2007Co-Authors: Thaddeus Chukwuemeka Ezeji, Nasib Qureshi, Hanspeter M BlaschekAbstract:During pretreatment and hydrolysis of fiber-rich agricultural biomass, compounds such as salts, furfural, hydroxymethyl furfural (HMF), acetic, ferulic, glucuronic, rho-coumaric acids, and phenolic compounds are produced. Clostridium beijerinckii BA101 can utilize the individual sugars present in lignocellulosic [e.g., corn fiber, distillers dry grain solubles (DDGS), etc] hydrolysates such as cellobiose, glucose, mannose, arabinose, and xylose. In these studies we investigated the effect of some of the lignocellulosic hydrolysate inhibitors associated with C. beijerinckii BA101 growth and acetone-butanol-ethanol (ABE) production. When 0.3 g/L rho-coumaric and ferulic acids were introduced into the fermentation medium, growth and ABE production by C. beijerinckii BA101 decreased significantly. Furfural and HMF are not inhibitory to C. beijerinckii BA101; rather they have stimulatory effect on the growth of the microorganism and ABE production.
-
recent advances in abe fermentation hyper butanol producing clostridium beijerinckii ba101
Journal of Industrial Microbiology & Biotechnology, 2001Co-Authors: N Qureshi, Hanspeter M BlaschekAbstract:This is an overview of the mutant strain Clostridium beijerinckii BA101 which produces solvents (acetone–butanol–ethanol, ABE) at elevated levels. This organism expresses high levels of amylases when grown on starch. C. beijerinckii BA101 hydrolyzes starch effectively and produces solvent in the concentration range of 27–29 g l−1. C. beijerinckii BA101 has been characterized for both substrate and butanol inhibition. Supplementing the fermentation medium (MP2) with sodium acetate enhances solvent production to 33 g l−1. The results of studies utilizing commercial fermentation medium and pilot plant-scale reactors are consistent with the results using small-scale reactors. Pervaporation, a technique to recover solvents, has been applied to fed-batch reactors containing C. beijerinckii BA101, and solvent production as high as 165 g l−1 has been achieved. Immobilization of C. beijerinckii BA101 by adsorption and use in a continuous reactor resulted in reactor productivity of 15.8 g l−1 h−1. Recent economic studies employing C. beijerinckii BA101 suggested that butanol can be produced at US$0.20–0.25 lb−1 by employing batch fermentation and distillative recovery. Application of new technologies such as pervaporation, fed-batch culture, and immobilized cell reactors is expected to further reduce these prices. Journal of Industrial Microbiology & Biotechnology (2001) 27, 287–291.
Hans P Blaschek - One of the best experts on this subject based on the ideXlab platform.
-
development of an oxygen independent flavin mononucleotide based fluorescent reporter system in clostridium beijerinckii and its potential applications
Journal of Biotechnology, 2018Co-Authors: Ting Lu, Hans P BlaschekAbstract:Abstract Clostridium beijerinckii is a predominant solventogenic clostridia with great attraction for renewable liquid biofuel and biochemical production. Metabolic engineering and synthetic biology can be employed to engineer the strain toward desirable phenotypes. However, current limited information such as promoter strength and gene regulation may hinder the efficient engineering of the strain. To investigate genetic information and complex cellular bioprocesses of C. beijerinckii, an in vivo fluorescence reporter system can be employed. In general, green fluorescence protein (GFP) and relative analogs have been widely used as real-time reporters. However, GFP-family proteins require molecular oxygen for fluorescence maturation. Considering the strict anaerobic growth requirement of the clostridia, an oxygen-independent fluorescence reporter such as a flavin mononucleotide-based fluorescent protein (FbFP) can be used as an alternative fluorescence reporter. In this study, we synthesized and expressed the codon-optimized FbFP gene for C. beijerinckii (CbFbFP) based on the nucleotide sequence of Bacillus subtilis YtvA variant EcFbFP in C. beijerinckii NCIMB 8052 wild-type. Protein expression and in vivo fluorescence of CbFbFP in C. beijeirnckii were confirmed under anaerobic growth conditions. Through fluorescence-activated cell sorting (FACS), we isolated the bright cells from the heterogenous population of C. beijerinckii cells expressing CbFbFP. Several mutations were found in the isolated plasmid which may be responsible for the high-level expression of CbFbFP in C. beijerinckii. The mutant plasmid and CbFbFP reporter were further utilized for strain selection, real-time fluorescence measurement, population analysis, and metabolic engineering in this study.
-
genome wide dynamic transcriptional profiling in clostridium beijerinckii ncimb 8052 using single nucleotide resolution rna seq
BMC Genomics, 2012Co-Authors: Yi Wang, Yuejian Mao, Hans P BlaschekAbstract:Clostridium beijerinckii is a prominent solvent-producing microbe that has great potential for biofuel and chemical industries. Although transcriptional analysis is essential to understand gene functions and regulation and thus elucidate proper strategies for further strain improvement, limited information is available on the genome-wide transcriptional analysis for C. beijerinckii. The genome-wide transcriptional dynamics of C. beijerinckii NCIMB 8052 over a batch fermentation process was investigated using high-throughput RNA-Seq technology. The gene expression profiles indicated that the glycolysis genes were highly expressed throughout the fermentation, with comparatively more active expression during acidogenesis phase. The expression of acid formation genes was down-regulated at the onset of solvent formation, in accordance with the metabolic pathway shift from acidogenesis to solventogenesis. The acetone formation gene (adc), as a part of the sol operon, exhibited highly-coordinated expression with the other sol genes. Out of the > 20 genes encoding alcohol dehydrogenase in C. beijerinckii, Cbei_1722 and Cbei_2181 were highly up-regulated at the onset of solventogenesis, corresponding to their key roles in primary alcohol production. Most sporulation genes in C. beijerinckii 8052 demonstrated similar temporal expression patterns to those observed in B. subtilis and C. acetobutylicum, while sporulation sigma factor genes sigE and sigG exhibited accelerated and stronger expression in C. beijerinckii 8052, which is consistent with the more rapid forespore and endspore development in this strain. Global expression patterns for specific gene functional classes were examined using self-organizing map analysis. The genes associated with specific functional classes demonstrated global expression profiles corresponding to the cell physiological variation and metabolic pathway switch. The results from this work provided insights for further C. beijerinckii strain improvement employing system biology-based strategies and metabolic engineering approaches.
-
single nucleotide resolution analysis of the transcriptome structure of clostridium beijerinckii ncimb 8052 using rna seq
BMC Genomics, 2011Co-Authors: Yi Wang, Yuejian Mao, Hans P BlaschekAbstract:Clostridium beijerinckii is an important solvent producing microorganism. The genome of C. beijerinckii NCIMB 8052 has recently been sequenced. Although transcriptome structure is important in order to reveal the functional and regulatory architecture of the genome, the physical structure of transcriptome for this strain, such as the operon linkages and transcript boundaries are not well understood. In this study, we conducted a single-nucleotide resolution analysis of the C. beijerinckii NCIMB 8052 transcriptome using high-throughput RNA-Seq technology. We identified the transcription start sites and operon structure throughout the genome. We confirmed the structure of important gene operons involved in metabolic pathways for acid and solvent production in C. beijerinckii 8052, including pta-ack, ptb-buk, hbd-etfA-etfB-crt (bcs) and ald-ctfA-ctfB-adc (sol) operons; we also defined important operons related to chemotaxis/motility, transcriptional regulation, stress response and fatty acids biosynthesis along with others. We discovered 20 previously non-annotated regions with significant transcriptional activities and 15 genes whose translation start codons were likely mis-annotated. As a consequence, the accuracy of existing genome annotation was significantly enhanced. Furthermore, we identified 78 putative silent genes and 177 putative housekeeping genes based on normalized transcription measurement with the sequence data. We also observed that more than 30% of pseudogenes had significant transcriptional activities during the fermentation process. Strong correlations exist between the expression values derived from RNA-Seq analysis and microarray data or qRT-PCR results. Transcriptome structural profiling in this research provided important supplemental information on the accuracy of genome annotation, and revealed additional gene functions and regulation in C. beijerinckii.
-
metabolic network reconstruction and genome scale model of butanol producing strain clostridium beijerinckii ncimb 8052
BMC Systems Biology, 2011Co-Authors: Caroline B Milne, Hans P Blaschek, Yong Su Jin, James A Eddy, Ravali Raju, Soroush Ardekani, Panjun Kim, Ryan S Senger, Nathan D PriceAbstract:Background Solventogenic clostridia offer a sustainable alternative to petroleum-based production of butanol--an important chemical feedstock and potential fuel additive or replacement. C. beijerinckii is an attractive microorganism for strain design to improve butanol production because it (i) naturally produces the highest recorded butanol concentrations as a byproduct of fermentation; and (ii) can co-ferment pentose and hexose sugars (the primary products from lignocellulosic hydrolysis). Interrogating C. beijerinckii metabolism from a systems viewpoint using constraint-based modeling allows for simulation of the global effect of genetic modifications.
-
transcriptional analysis of clostridium beijerinckii ncimb 8052 and the hyper butanol producing mutant ba101 during the shift from acidogenesis to solventogenesis
Applied and Environmental Microbiology, 2008Co-Authors: Zhen Shi, Hans P BlaschekAbstract:Clostridium beijerinckii is an anaerobic bacterium used for the fermentative production of acetone and butanol. The recent availability of genomic sequence information for C. beijerinckii NCIMB 8052 has allowed for an examination of gene expression during the shift from acidogenesis to solventogenesis over the time course of a batch fermentation using a ca. 500-gene set DNA microarray. The microarray was constructed using a collection of genes which are orthologs of members of gene families previously found to be important to the physiology of C. acetobutylicum ATCC 824. Similar to the onset of solventogenesis in C. acetobutylicum 824, the onset of solventogenesis in C. beijerinckii 8052 was concurrent with the initiation of sporulation. However, forespores and endospores developed more rapidly in C. beijerinckii 8052 than in C. acetobutylicum 824, consistent with the accelerated expression of the sigE- and sigG-regulated genes in C. beijerinckii 8052. The comparison of gene expression patterns and morphological changes in C. beijerinckii 8052 and the hyper-butanol-producing C. beijerinckii strain BA101 indicated that BA101 was less efficient in sporulation and phosphotransferase system-mediated sugar transport than 8052 but that it exhibited elevated expression of several primary metabolic genes and chemotaxis/motility genes.
Thaddeus Chukwuemeka Ezeji - One of the best experts on this subject based on the ideXlab platform.
-
identification purification and characterization of furfural transforming enzymes from clostridium beijerinckii ncimb 8052
Anaerobe, 2015Co-Authors: Yan Zhang, Victor Ujor, Macdonald Wick, Thaddeus Chukwuemeka EzejiAbstract:Abstract Generation of microbial inhibitory compounds such as furfural and 5-hydroxymethylfurfural (HMF) is a formidable roadblock to fermentation of lignocellulose-derived sugars to butanol. Bioabatement offers a cost effective strategy to circumvent this challenge. Although Clostridium beijerinckii NCIMB 8052 can transform 2–3 g/L of furfural and HMF to their less toxic alcohols, higher concentrations present in biomass hydrolysates are intractable to microbial transformation. To delineate the mechanism by which C. beijerinckii detoxifies furfural and HMF, an aldo/keto reductase (AKR) and a short-chain dehydrogenase/reductase (SDR) found to be over-expressed in furfural-challenged cultures of C. beijerinckii were cloned and over-expressed in Escherichia coli Rosetta-gami™ B(DE3)pLysS, and purified by histidine tag-assisted immobilized metal affinity chromatography. Protein gel analysis showed that the molecular weights of purified AKR and SDR are close to the predicted values of 37 kDa and 27 kDa, respectively. While AKR has apparent Km and Vmax values of 32.4 mM and 254.2 mM s−1 respectively, using furfural as substrate, SDR showed lower Km (26.4 mM) and Vmax (22.6 mM s−1) values on the same substrate. However, AKR showed 7.1-fold higher specific activity on furfural than SDR. Further, both AKR and SDR were found to be active on HMF, benzaldehyde, and butyraldehyde. Both enzymes require NADPH as a cofactor for aldehydes reduction. Based on these results, it is proposed that AKR and SDR are involved in the biotransformation of furfural and HMF by C. beijerinckii.
-
allopurinol mediated lignocellulose derived microbial inhibitor tolerance by clostridium beijerinckii during acetone butanol ethanol abe fermentation
Applied Microbiology and Biotechnology, 2015Co-Authors: Victor Ujor, Chidozie Victor Agu, Venkat Gopalan, Thaddeus Chukwuemeka EzejiAbstract:In addition to glucans, xylans, and arabinans, lignocellulosic biomass hydrolysates contain significant levels of nonsugar components that are toxic to the microbes that are typically used to convert biomass to biofuels and chemicals. To enhance the tolerance of acetone–butanol–ethanol (ABE)-generating Clostridium beijerinckii NCIMB 8052 to these lignocellulose-derived microbial inhibitory compounds (LDMICs; e.g., furfural), we have been examining different metabolic perturbation strategies to increase the cellular reductant pools and thereby facilitate detoxification of LDMICs. As part of these efforts, we evaluated the effect of allopurinol, an inhibitor of NAD(P)H-generating xanthine dehydrogenase (XDH), on C. beijerinckii grown in furfural-supplemented medium and found that it unexpectedly increased the rate of detoxification of furfural by 1.4-fold and promoted growth, butanol, and ABE production by 1.2-, 2.5-, and 2-fold, respectively. Since NAD(P)H/NAD(P)+ levels in C. beijerinckii were largely unchanged upon allopurinol treatment, we postulated and validated a possible basis in DNA repair to account for the solventogenic gains with allopurinol. Following the observation that supplementation of allopurinol in the C. beijerinckii growth media mitigates the toxic effects of nalidixic acid, a DNA-damaging antibiotic, we found that allopurinol elicited 2.4- and 6.7-fold increase in the messenger RNA (mRNA) levels of xanthine and hypoxanthine phosphoribosyltransferases, key purine-salvage enzymes. Consistent with this finding, addition of inosine (a precursor of hypoxanthine) and xanthine led to 1.4- and 1.7-fold increase in butanol production in furfural-challenged cultures of C. beijerinckii. Taken together, our results provide a purine salvage-based rationale for the unanticipated effect of allopurinol in improving furfural tolerance of the ABE-fermenting C. beijerinckii.
-
transcriptional analysis of clostridium beijerinckii ncimb 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation
Biotechnology for Biofuels, 2013Co-Authors: Yan Zhang, Thaddeus Chukwuemeka EzejiAbstract:Furfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulose biomass to monomeric sugars, but the response of acetone butanol ethanol (ABE) producing Clostridium beijerinckii NCIMB 8052 to this compound at the molecular level is unknown. To discern the effect of furfural on C. beijerinckii and to gain insight into molecular mechanisms of action and detoxification, physiological changes of furfural-stressed cultures during acetone butanol ethanol (ABE) fermentation were studied, and differentially expressed genes were profiled by genome-wide transcriptional analysis. A total of 5,003 C. beijerinckii NCIMB 8052 genes capturing about 99.7% of the genome were examined. About 111 genes were differentially expressed (up- or down-regulated) by C. beijerinckii when it was challenged with furfural at acidogenic growth phase compared with 721 genes that were differentially expressed (up- or down-regulated) when C. beijerinckii was challenged with furfural at solventogenic growth phase. The differentially expressed genes include genes related to redox and cofactors, membrane transporters, carbohydrate, amino sugar and nucleotide sugar metabolisms, heat shock proteins, DNA repair, and two-component signal transduction system. While C. beijerinckii exposed to furfural stress during the acidogenic growth phase produced 13% more ABE than the unstressed control, ABE production by C. beijerinckii ceased following exposure to furfural stress during the solventogenic growth phase. Genome-wide transcriptional response of C. beijerinckii to furfural stress was investigated for the first time using microarray analysis. Stresses emanating from ABE accumulation in the fermentation medium; redox balance perturbations; and repression of genes that code for the phosphotransferase system, cell motility and flagellar proteins (and combinations thereof) may have caused the premature termination of C. beijerinckii 8052 growth and ABE production following furfural challenge at the solventogenic phase.This study provides insights into basis for metabolic engineering of C. beijerinckii NCIMB 8052 for enhanced tolerance of lignocellulose-derived microbial inhibitory compounds, thereby improving bioconversion of lignocellulose biomass hydrolysates to biofuels and chemicals. Indeed, two enzymes encoded by Cbei_3974 and Cbei_3904 belonging to aldo/keto reductase (AKR) and short-chain dehydrogenase/reductase (SDR) families have been identified to be involved in furfural detoxification and tolerance.
-
Impact of syringaldehyde on the growth of Clostridium beijerinckii NCIMB 8052 and butanol production
3 Biotech, 2012Co-Authors: Catherine Richmond, Victor Ujor, Thaddeus Chukwuemeka EzejiAbstract:While lignocellulosic biomass excels as a cheap, renewable resource for biofuel production, it does present some challenges such as generation of microbial inhibitory compounds. The mode of selective inhibition of acetone–butanol–ethanol (ABE) production (as opposed to cell growth) by syringaldehyde on Clostridium beijerinckii NCIMB 8052 was examined. C. beijerinckii 8052 grown in syringaldehyde-supplemented P2 medium had a comparable growth rate (μ = 0.34) at acidogenic growth phase to that of C. beijerinckii 8052 grown in control P2 medium (μ = 0.30). The addition of syringaldehyde into P2 medium inhibited solvent production by C. beijerinckii 8052 and increased butyric and acetic acid accumulation in the fermentation broth. Analysis of coenzyme A transferase (CoAT) using cell-free extracts of C. beijerinckii 8052 showed decreased expression and activity in the presence of syringaldehyde. These results indicate that C. beijerinckii 8052 CoAT is negatively affected by syringaldehyde and thus, hampers the ability of the microorganism to metabolize butyric and acetic acid for ABE production as evidenced by the accumulation of butyric and acetic acid in the fermentation broth.
-
butanol production from agricultural residues impact of degradation products on clostridium beijerinckii growth and butanol fermentation
Biotechnology and Bioengineering, 2007Co-Authors: Thaddeus Chukwuemeka Ezeji, Nasib Qureshi, Hanspeter M BlaschekAbstract:During pretreatment and hydrolysis of fiber-rich agricultural biomass, compounds such as salts, furfural, hydroxymethyl furfural (HMF), acetic, ferulic, glucuronic, ρ-coumaric acids, and phenolic compounds are produced. Clostridium beijerinckii BA101 can utilize the individual sugars present in lignocellulosic [e.g., corn fiber, distillers dry grain solubles (DDGS), etc] hydrolysates such as cellobiose, glucose, mannose, arabinose, and xylose. In these studies we investigated the effect of some of the lignocellulosic hydrolysate inhibitors associated with C. beijerinckii BA101 growth and acetone–butanol–ethanol (ABE) production. When 0.3 g/L ρ-coumaric and ferulic acids were introduced into the fermentation medium, growth and ABE production by C. beijerinckii BA101 decreased significantly. Furfural and HMF are not inhibitory to C. beijerinckii BA101; rather they have stimulatory effect on the growth of the microorganism and ABE production. Biotechnol. Bioeng. 2007;97:1460–1469. © 2007 Wiley Periodicals, Inc.
Adilma Regina Pippa Scamparini - One of the best experts on this subject based on the ideXlab platform.
-
Exopolysaccharide production by encapsulated Beijerinckia cultures
Process Biochemistry, 2004Co-Authors: Y. Boza, L.p Neto, F.a.a Costa, Adilma Regina Pippa ScampariniAbstract:Abstract It is desirable that spray-dried microorganisms maintain their viability and activity during encapsulation, a possibility that depends on the strain and the conditions of encapsulation. A wide variety of modifications may occur in cells after encapsulation. This work examines the effects of encapsulation in malt dextrin on the production of biopolymer, comparing encapsulated and non-encapsulated Beijerinckia cells. From the characterisation of the biopolymers and study of the fermentation kinetics, it was shown that the encapsulation of Beijerinckia mainly influenced cell growth and biopolymer production.
-
effect of spray drying on the quality of encapsulated cells of Beijerinckia sp
Process Biochemistry, 2004Co-Authors: Y. Boza, Decio Barbin, Adilma Regina Pippa ScampariniAbstract:The survival and fermentative activity of Beijerinckia sp cultures encapsulated in malt dextrin by spray-drying were determined under various conditions of spray-drying and during the subsequent storage period of the products. The survival of Beijerinckia sp was greater with lower outlet air temperatures and with lower solids contents in the feed suspension during spray-drying. Increasing either the spray dryer outlet air temperature or the feed solids content resulted in a decrease in the moisture content of the spray dried powders. A gradual decrease in viability was observed during storage for all treatments, although there was a relatively greater retention of fermenting activity in the inocula obtained with lower outlet air temperatures and lower solids contents in the feed suspension.
-
encapsulamento de Beijerinckia sp utilizando spray drier
2003Co-Authors: Y. Boza, Adilma Regina Pippa ScampariniAbstract:A escolha adequada do material de parede e um dos fatores determin antes no grau de viabilidade e na manutencao da atividade fermentativa do inoculo encapsulado. Maltodextrina, glicose, amido modificado e goma arabica foram testadas como encapsulantes. Beijerinckiaencapsulada em maltodextrina, reidratada e inoculada em meio de fermentacao previamente esterilizado, apos um periodo de estocagem de dois meses, apresentou maior estabilidade da atividade fermentativa. A sobrevivencia e a atividade fermentativa das celulas de Beijerinckia encapsulada em maltodextrina foram tes tadas sob varias condicoes do spray-drier e durante posterior periodo de estocagem. A viabilidade bacteriana para todas condicoes testadas apresentou declinio porem quando se utilizou menores temperatura de saida no spray -drier e teor de solidos na suspen sao obteve-se maior grau de sobrevivencia. Para verificar a influencia da encapsulacao em maltodextrina sobre a producao de biopolimero comparou -se a atividade fermentativa das celulas de Beijerinckia encapsulada e nao encapsulada. A partir das caracterist icas reologicas e quimicas dos biopolimeros produzidos e da cinetica fermentativa dos inoculos concluiuise que encapsulacao de Beijerinckia influe no crescimento celular e na producao de biopolimero%%%%An adequate choice of the wall (coating) material is one of the factors, which will determine the degree of cell survival and the maintenance of fermentative activity in the encapsulated inoculum. Malt dextrin, dehydrated glucose syrups, modified starch and acacia (gum Arabic) were used as wall ma terials. The results showed that spray dried Beijerinckia encapsulated in malt dextrin, stored for two months and inoculated into sterile must after re -hydration, presented the greatest stability related to fermentative activity and percent viability durin 9 spray drying and storage period. The survival and fermentative activity of Beijerinckia sp cultures encapsulated in malt dextrin by spray drying were determined under various conditions of spray drying and during the subsequent storage period of the products. The survival of Beijerinckia sp was greater with lower outlet air temperatures and with lower solids contents in the feed suspension during spray drying. Increasing either the spray dryer outlet air temperature or the feed solids content resulted in a decrease in the moisture content of the spray dried powders. A gradual decrease in viability was observed during storage for all treatments, although there was a relatively greater retention of fermenting activity in the inoculae obtained with lower outlet air temperatures and lower solids contents in the feed suspension. Was to examine the effects of encapsulation in malt dextrin on the production of biopolymer, comparing encapsulated and non-encapsulated Beijerinckia cells. From the characterization of the biopolymers and study of the fermentation kinetics, it was shown that the encapsulation of Beijerinckia mainly influenced cell growth and biopolymer production
-
activity and survival of spray dried Beijerinckia sp microencapsulated in different carbohydrates
Applied Biochemistry and Biotechnology, 2003Co-Authors: Y. Boza, Decio Barbin, Adilma Regina Pippa ScampariniAbstract:This study examined the possibility of preserving Beijerinckia cultures by encapsulation using a spray drier, for use in biotechnological processes in the production of biopolymers. An adequate choice of the wall (coating) material is one of the factors that will determine the degree of cell survival and the maintenance of fermentative activity in the encapsulated inoculum. Malt dextrin, dehydrated glucose syrups, modified starch, and acacia (gum arabic) were used as wall materials. The results showed that spray-dried Beijerinckia encapsulated in malt dextrin, stored for 2 mo, and inoculated into sterile must after rehydration presented the greates stability with respect to fermentative activity, although the glucose-encap sulated cells showed the highest percentage of viability during spray drying and during the storage period.
Y. Boza - One of the best experts on this subject based on the ideXlab platform.
-
Encapsulamento de Beijerinckia sp utilizando spray-drier
2017Co-Authors: Y. BozaAbstract:Resumo: A escolha adequada do material de parede é um dos fatores determin antes no grau de viabilidade e na manutenção da atividade fermentativa do inóculo encapsulado. Maltodextrina, glicose, amido modificado e goma arábica foram testadas como encapsulantes. Beijerínckíaencapsulada em maltodextrina, reidratada e inoculada em meio de fermentação previamente esterilizado, após um período de estocagem de dois meses, apresentou maior estabilidade da atividade fermentativa. A sobrevivencia e a atividade fermentativa das células de Beijerínckía encapsulada em maltodextrina foram tes tadas sob várias condições do spray-drier e durante posterior período de estocagem. A viabilidade bacteriana para todas condições testadas apresentou declinio pórem quando se utilizou menores temperatura de saída no spray -drier e teor de sólidos na suspen são obteve-se maior grau de sobrevivência. Para verificar a influência da encapsulação em maltodextrina sobre a produção de biopolímero comparou -se a atividade fermentativa das células de Beijerínckía encapsulada e não encapsulada. A partir das característ icas reológicas e químicas dos biopolímeros produzidos e da cinética fermentativa dos inóculos concluiuise que encapsulaçao de Beijerínckía influe no crescimento celular e na produção de biopolímeroAbstract:An adequate choice of the wall (coating) material is one of the factors, which will determine the degree of cell survival and the maintenance of fermentative activity in the encapsulated inoculum. Malt dextrin, dehydrated glucose syrups, modified starch and acacia (gum Arabic) were used as wall ma terials. The results showed that spray dried Beijerinckia encapsulated in malt dextrin, stored for two months and inoculated into sterile must after re -hydration, presented the greatest stability related to fermentative activity and percent viability durin 9 spray drying and storage period. The survival and fermentative activity of Beijerinckia sp cultures encapsulated in malt dextrin by spray drying were determined under various conditions of spray drying and during the subsequent storage period of the products. The survival of Beijerinckia sp was greater with lower outlet air temperatures and with lower solids contents in the feed suspension during spray drying. Increasing either the spray dryer outlet air temperature or the feed solids content resulted in a decrease in the moisture content of the spray dried powders. A gradual decrease in viability was observed during storage for all treatments, although there was a relatively greater retention of fermenting activity in the inoculae obtained with lower outlet air temperatures and lower solids contents in the feed suspension. Was to examine the effects of encapsulation in malt dextrin on the production of biopolymer, comparing encapsulated and non-encapsulated Beijerinckia cells. From the characterization of the biopolymers and study of the fermentation kinetics, it was shown that the encapsulation of Beijerinckia mainly influenced cell growth and biopolymer productio
-
Exopolysaccharide production by encapsulated Beijerinckia cultures
Process Biochemistry, 2004Co-Authors: Y. Boza, L.p Neto, F.a.a Costa, Adilma Regina Pippa ScampariniAbstract:Abstract It is desirable that spray-dried microorganisms maintain their viability and activity during encapsulation, a possibility that depends on the strain and the conditions of encapsulation. A wide variety of modifications may occur in cells after encapsulation. This work examines the effects of encapsulation in malt dextrin on the production of biopolymer, comparing encapsulated and non-encapsulated Beijerinckia cells. From the characterisation of the biopolymers and study of the fermentation kinetics, it was shown that the encapsulation of Beijerinckia mainly influenced cell growth and biopolymer production.
-
effect of spray drying on the quality of encapsulated cells of Beijerinckia sp
Process Biochemistry, 2004Co-Authors: Y. Boza, Decio Barbin, Adilma Regina Pippa ScampariniAbstract:The survival and fermentative activity of Beijerinckia sp cultures encapsulated in malt dextrin by spray-drying were determined under various conditions of spray-drying and during the subsequent storage period of the products. The survival of Beijerinckia sp was greater with lower outlet air temperatures and with lower solids contents in the feed suspension during spray-drying. Increasing either the spray dryer outlet air temperature or the feed solids content resulted in a decrease in the moisture content of the spray dried powders. A gradual decrease in viability was observed during storage for all treatments, although there was a relatively greater retention of fermenting activity in the inocula obtained with lower outlet air temperatures and lower solids contents in the feed suspension.
-
encapsulamento de Beijerinckia sp utilizando spray drier
2003Co-Authors: Y. Boza, Adilma Regina Pippa ScampariniAbstract:A escolha adequada do material de parede e um dos fatores determin antes no grau de viabilidade e na manutencao da atividade fermentativa do inoculo encapsulado. Maltodextrina, glicose, amido modificado e goma arabica foram testadas como encapsulantes. Beijerinckiaencapsulada em maltodextrina, reidratada e inoculada em meio de fermentacao previamente esterilizado, apos um periodo de estocagem de dois meses, apresentou maior estabilidade da atividade fermentativa. A sobrevivencia e a atividade fermentativa das celulas de Beijerinckia encapsulada em maltodextrina foram tes tadas sob varias condicoes do spray-drier e durante posterior periodo de estocagem. A viabilidade bacteriana para todas condicoes testadas apresentou declinio porem quando se utilizou menores temperatura de saida no spray -drier e teor de solidos na suspen sao obteve-se maior grau de sobrevivencia. Para verificar a influencia da encapsulacao em maltodextrina sobre a producao de biopolimero comparou -se a atividade fermentativa das celulas de Beijerinckia encapsulada e nao encapsulada. A partir das caracterist icas reologicas e quimicas dos biopolimeros produzidos e da cinetica fermentativa dos inoculos concluiuise que encapsulacao de Beijerinckia influe no crescimento celular e na producao de biopolimero%%%%An adequate choice of the wall (coating) material is one of the factors, which will determine the degree of cell survival and the maintenance of fermentative activity in the encapsulated inoculum. Malt dextrin, dehydrated glucose syrups, modified starch and acacia (gum Arabic) were used as wall ma terials. The results showed that spray dried Beijerinckia encapsulated in malt dextrin, stored for two months and inoculated into sterile must after re -hydration, presented the greatest stability related to fermentative activity and percent viability durin 9 spray drying and storage period. The survival and fermentative activity of Beijerinckia sp cultures encapsulated in malt dextrin by spray drying were determined under various conditions of spray drying and during the subsequent storage period of the products. The survival of Beijerinckia sp was greater with lower outlet air temperatures and with lower solids contents in the feed suspension during spray drying. Increasing either the spray dryer outlet air temperature or the feed solids content resulted in a decrease in the moisture content of the spray dried powders. A gradual decrease in viability was observed during storage for all treatments, although there was a relatively greater retention of fermenting activity in the inoculae obtained with lower outlet air temperatures and lower solids contents in the feed suspension. Was to examine the effects of encapsulation in malt dextrin on the production of biopolymer, comparing encapsulated and non-encapsulated Beijerinckia cells. From the characterization of the biopolymers and study of the fermentation kinetics, it was shown that the encapsulation of Beijerinckia mainly influenced cell growth and biopolymer production
-
activity and survival of spray dried Beijerinckia sp microencapsulated in different carbohydrates
Applied Biochemistry and Biotechnology, 2003Co-Authors: Y. Boza, Decio Barbin, Adilma Regina Pippa ScampariniAbstract:This study examined the possibility of preserving Beijerinckia cultures by encapsulation using a spray drier, for use in biotechnological processes in the production of biopolymers. An adequate choice of the wall (coating) material is one of the factors that will determine the degree of cell survival and the maintenance of fermentative activity in the encapsulated inoculum. Malt dextrin, dehydrated glucose syrups, modified starch, and acacia (gum arabic) were used as wall materials. The results showed that spray-dried Beijerinckia encapsulated in malt dextrin, stored for 2 mo, and inoculated into sterile must after rehydration presented the greates stability with respect to fermentative activity, although the glucose-encap sulated cells showed the highest percentage of viability during spray drying and during the storage period.