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Surendra Prakash Goyal - One of the best experts on this subject based on the ideXlab platform.

  • fine scale population genetic structure of the Bengal Tiger panthera tigris tigris in a human dominated western terai arc landscape india
    PLOS ONE, 2017
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Puneet Pandey, Reeta Sharma, Randeep Singh, Manoj Agrawal, Surendra Prakash Goyal
    Abstract:

    Despite massive global conservation strategies, Tiger populations continued to decline until recently, mainly due to habitat loss, human-animal conflicts, and poaching. These factors are known to affect the genetic characteristics of Tiger populations and decrease local effective population sizes. The Terai Arc Landscape (TAL) at the foothills of the Himalaya is one of the 42 source sites of Tigers around the globe. Therefore, information on how landscape features and anthropogenic factors affect the fine-scale spatial genetic structure and variation of Tigers in TAL is needed to develop proper management strategies for achieving long-term conservation goals. We document, for the first time, the genetic characteristics of this Tiger population by genotyping 71 Tiger samples using 13 microsatellite markers from the western region of TAL (WTAL) of 1800 km2. Specifically, we aimed to estimate the genetic variability, population structure, and gene flow. The microsatellite markers indicated that the levels of allelic diversity (MNA = 6.6) and genetic variation (Ho = 0.50, HE = 0.64) were slightly lower than those reported previously in other Bengal Tiger populations. We observed moderate gene flow and significant genetic differentiation (FST= 0.060) and identified the presence of cryptic genetic structure using Bayesian and non-Bayesian approaches. There was low and significantly asymmetric migration between the two main subpopulations of the Rajaji Tiger Reserve and the Corbett Tiger Reserve in WTAL. Sibship relationships indicated that the functionality of the corridor between these subpopulations may be retained if the quality of the habitat does not deteriorate. However, we found that gene flow is not adequate in view of changing land use matrices. We discuss the need to maintain connectivity by implementing the measures that have been suggested previously to minimize the level of human disturbance, including relocation of villages and industries, prevention of encroachment, and banning sand and boulder mining in the corridors.

  • Comparison of ecological exchangeability between Sundarbans and mainland Bengal Tiger landscape in India.
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    Comparison of ecological exchangeability between Sundarbans and mainland Bengal Tiger landscape in India.

  • Allele size range, Number of alleles (Na) and genotyping error rates (ADO = allelic dropout, FA = False allele) at nine microsatellite loci with field collected scat (n = 10; for Kanha Tiger reserve), tissue samples (n = 10; from Corbett Tiger reserv
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    Allele size range, Number of alleles (Na) and genotyping error rates (ADO = allelic dropout, FA = False allele) at nine microsatellite loci with field collected scat (n = 10; for Kanha Tiger reserve), tissue samples (n = 10; from Corbett Tiger reserve) and scat & hair samples from Sundarbans (n = 8) of wild Bengal Tiger.

  • Median-joining network created from four mtDNA genes (cytb, ND2, ND5 and ND6) (in total, 2600 bp) depicting genetic relationship between all haplotypes found in Tigers.
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    (a) haplotypes found in Sundarbans Tigers (in black) and all other six Tiger subspecies (in yellow and green color, from Luo et al. 2004) [2], (b) all haplotypes found in Bengal Tiger populations from this study and Mondol et al. [22]. Pink: North India, Yellow: Central India, Blue: South India, and Green: Sundarbans. The sizes of the circles are proportional to the haplotype frequencies.

  • Panel of polymorphic heterologous microsatellite loci to genotype critically endangered Bengal Tiger: a pilot study
    SpringerPlus, 2014
    Co-Authors: Sudhanshu Mishra, Sujeet Kumar Singh, Jouni Aspi, Ashok Kumar Munjal, Surendra Prakash Goyal
    Abstract:

    In India, six landscapes and source populations that are important for long-term conservation of Bengal Tigers ( Panthera tigris tigris ) have been identified. Except for a few studies, nothing is known regarding the genetic structure and extent of gene flow among most of the Tiger populations across India as the majority of them are small, fragmented and isolated. Thus, individual-based relationships are required to understand the species ecology and biology for planning effective conservation and genetics-based individual identification has been widely used. But this needs screening and describing characteristics of microsatellite loci from DNA from good-quality sources so that the required number of loci can be selected and the genotyping error rate minimized. In the studies so far conducted on the Bengal Tiger, a very small number of loci (n = 35) have been tested with high-quality source of DNA, and information on locus-specific characteristics is lacking. The use of such characteristics has been strongly recommended in the literature to minimize the error rate and by the International Society for Forensic Genetics (ISFG) for forensic purposes. Therefore, we describe for the first time locus-specific genetic and genotyping profile characteristics, crucial for population genetic studies, using high-quality source of DNA of the Bengal Tiger. We screened 39 heterologous microsatellite loci (Sumatran Tiger, domestic cat, Asiatic lion and snow leopard) in captive individuals (n = 8), of which 21 loci are being reported for the first time in the Bengal Tiger, providing an additional choice for selection. The mean relatedness coefficient (R = −0.143) indicates that the selected Tigers were unrelated. Thirty-four loci were polymorphic, with the number of alleles ranging from 2 to 7 per locus, and the remaining five loci were monomorphic. Based on the PIC values (> 0.500), and other characteristics, we suggest that 16 loci (3 to 7 alleles) be used for genetic and forensic study purposes. The probabilities of matching genotypes of unrelated individuals (3.692 × 10^-19) and siblings (4.003 × 10^-6) are within the values needed for undertaking studies in population genetics, relatedness, sociobiology and forensics.

Sujeet Kumar Singh - One of the best experts on this subject based on the ideXlab platform.

  • fine scale population genetic structure of the Bengal Tiger panthera tigris tigris in a human dominated western terai arc landscape india
    PLOS ONE, 2017
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Puneet Pandey, Reeta Sharma, Randeep Singh, Manoj Agrawal, Surendra Prakash Goyal
    Abstract:

    Despite massive global conservation strategies, Tiger populations continued to decline until recently, mainly due to habitat loss, human-animal conflicts, and poaching. These factors are known to affect the genetic characteristics of Tiger populations and decrease local effective population sizes. The Terai Arc Landscape (TAL) at the foothills of the Himalaya is one of the 42 source sites of Tigers around the globe. Therefore, information on how landscape features and anthropogenic factors affect the fine-scale spatial genetic structure and variation of Tigers in TAL is needed to develop proper management strategies for achieving long-term conservation goals. We document, for the first time, the genetic characteristics of this Tiger population by genotyping 71 Tiger samples using 13 microsatellite markers from the western region of TAL (WTAL) of 1800 km2. Specifically, we aimed to estimate the genetic variability, population structure, and gene flow. The microsatellite markers indicated that the levels of allelic diversity (MNA = 6.6) and genetic variation (Ho = 0.50, HE = 0.64) were slightly lower than those reported previously in other Bengal Tiger populations. We observed moderate gene flow and significant genetic differentiation (FST= 0.060) and identified the presence of cryptic genetic structure using Bayesian and non-Bayesian approaches. There was low and significantly asymmetric migration between the two main subpopulations of the Rajaji Tiger Reserve and the Corbett Tiger Reserve in WTAL. Sibship relationships indicated that the functionality of the corridor between these subpopulations may be retained if the quality of the habitat does not deteriorate. However, we found that gene flow is not adequate in view of changing land use matrices. We discuss the need to maintain connectivity by implementing the measures that have been suggested previously to minimize the level of human disturbance, including relocation of villages and industries, prevention of encroachment, and banning sand and boulder mining in the corridors.

  • Comparison of ecological exchangeability between Sundarbans and mainland Bengal Tiger landscape in India.
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    Comparison of ecological exchangeability between Sundarbans and mainland Bengal Tiger landscape in India.

  • Allele size range, Number of alleles (Na) and genotyping error rates (ADO = allelic dropout, FA = False allele) at nine microsatellite loci with field collected scat (n = 10; for Kanha Tiger reserve), tissue samples (n = 10; from Corbett Tiger reserv
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    Allele size range, Number of alleles (Na) and genotyping error rates (ADO = allelic dropout, FA = False allele) at nine microsatellite loci with field collected scat (n = 10; for Kanha Tiger reserve), tissue samples (n = 10; from Corbett Tiger reserve) and scat & hair samples from Sundarbans (n = 8) of wild Bengal Tiger.

  • Median-joining network created from four mtDNA genes (cytb, ND2, ND5 and ND6) (in total, 2600 bp) depicting genetic relationship between all haplotypes found in Tigers.
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    (a) haplotypes found in Sundarbans Tigers (in black) and all other six Tiger subspecies (in yellow and green color, from Luo et al. 2004) [2], (b) all haplotypes found in Bengal Tiger populations from this study and Mondol et al. [22]. Pink: North India, Yellow: Central India, Blue: South India, and Green: Sundarbans. The sizes of the circles are proportional to the haplotype frequencies.

  • Panel of polymorphic heterologous microsatellite loci to genotype critically endangered Bengal Tiger: a pilot study
    SpringerPlus, 2014
    Co-Authors: Sudhanshu Mishra, Sujeet Kumar Singh, Jouni Aspi, Ashok Kumar Munjal, Surendra Prakash Goyal
    Abstract:

    In India, six landscapes and source populations that are important for long-term conservation of Bengal Tigers ( Panthera tigris tigris ) have been identified. Except for a few studies, nothing is known regarding the genetic structure and extent of gene flow among most of the Tiger populations across India as the majority of them are small, fragmented and isolated. Thus, individual-based relationships are required to understand the species ecology and biology for planning effective conservation and genetics-based individual identification has been widely used. But this needs screening and describing characteristics of microsatellite loci from DNA from good-quality sources so that the required number of loci can be selected and the genotyping error rate minimized. In the studies so far conducted on the Bengal Tiger, a very small number of loci (n = 35) have been tested with high-quality source of DNA, and information on locus-specific characteristics is lacking. The use of such characteristics has been strongly recommended in the literature to minimize the error rate and by the International Society for Forensic Genetics (ISFG) for forensic purposes. Therefore, we describe for the first time locus-specific genetic and genotyping profile characteristics, crucial for population genetic studies, using high-quality source of DNA of the Bengal Tiger. We screened 39 heterologous microsatellite loci (Sumatran Tiger, domestic cat, Asiatic lion and snow leopard) in captive individuals (n = 8), of which 21 loci are being reported for the first time in the Bengal Tiger, providing an additional choice for selection. The mean relatedness coefficient (R = −0.143) indicates that the selected Tigers were unrelated. Thirty-four loci were polymorphic, with the number of alleles ranging from 2 to 7 per locus, and the remaining five loci were monomorphic. Based on the PIC values (> 0.500), and other characteristics, we suggest that 16 loci (3 to 7 alleles) be used for genetic and forensic study purposes. The probabilities of matching genotypes of unrelated individuals (3.692 × 10^-19) and siblings (4.003 × 10^-6) are within the values needed for undertaking studies in population genetics, relatedness, sociobiology and forensics.

Reeta Sharma - One of the best experts on this subject based on the ideXlab platform.

  • fine scale population genetic structure of the Bengal Tiger panthera tigris tigris in a human dominated western terai arc landscape india
    PLOS ONE, 2017
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Puneet Pandey, Reeta Sharma, Randeep Singh, Manoj Agrawal, Surendra Prakash Goyal
    Abstract:

    Despite massive global conservation strategies, Tiger populations continued to decline until recently, mainly due to habitat loss, human-animal conflicts, and poaching. These factors are known to affect the genetic characteristics of Tiger populations and decrease local effective population sizes. The Terai Arc Landscape (TAL) at the foothills of the Himalaya is one of the 42 source sites of Tigers around the globe. Therefore, information on how landscape features and anthropogenic factors affect the fine-scale spatial genetic structure and variation of Tigers in TAL is needed to develop proper management strategies for achieving long-term conservation goals. We document, for the first time, the genetic characteristics of this Tiger population by genotyping 71 Tiger samples using 13 microsatellite markers from the western region of TAL (WTAL) of 1800 km2. Specifically, we aimed to estimate the genetic variability, population structure, and gene flow. The microsatellite markers indicated that the levels of allelic diversity (MNA = 6.6) and genetic variation (Ho = 0.50, HE = 0.64) were slightly lower than those reported previously in other Bengal Tiger populations. We observed moderate gene flow and significant genetic differentiation (FST= 0.060) and identified the presence of cryptic genetic structure using Bayesian and non-Bayesian approaches. There was low and significantly asymmetric migration between the two main subpopulations of the Rajaji Tiger Reserve and the Corbett Tiger Reserve in WTAL. Sibship relationships indicated that the functionality of the corridor between these subpopulations may be retained if the quality of the habitat does not deteriorate. However, we found that gene flow is not adequate in view of changing land use matrices. We discuss the need to maintain connectivity by implementing the measures that have been suggested previously to minimize the level of human disturbance, including relocation of villages and industries, prevention of encroachment, and banning sand and boulder mining in the corridors.

  • Comparison of ecological exchangeability between Sundarbans and mainland Bengal Tiger landscape in India.
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    Comparison of ecological exchangeability between Sundarbans and mainland Bengal Tiger landscape in India.

  • Allele size range, Number of alleles (Na) and genotyping error rates (ADO = allelic dropout, FA = False allele) at nine microsatellite loci with field collected scat (n = 10; for Kanha Tiger reserve), tissue samples (n = 10; from Corbett Tiger reserv
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    Allele size range, Number of alleles (Na) and genotyping error rates (ADO = allelic dropout, FA = False allele) at nine microsatellite loci with field collected scat (n = 10; for Kanha Tiger reserve), tissue samples (n = 10; from Corbett Tiger reserve) and scat & hair samples from Sundarbans (n = 8) of wild Bengal Tiger.

  • Median-joining network created from four mtDNA genes (cytb, ND2, ND5 and ND6) (in total, 2600 bp) depicting genetic relationship between all haplotypes found in Tigers.
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    (a) haplotypes found in Sundarbans Tigers (in black) and all other six Tiger subspecies (in yellow and green color, from Luo et al. 2004) [2], (b) all haplotypes found in Bengal Tiger populations from this study and Mondol et al. [22]. Pink: North India, Yellow: Central India, Blue: South India, and Green: Sundarbans. The sizes of the circles are proportional to the haplotype frequencies.

  • Genetically distinct population of Bengal Tiger (Panthera tigris tigris) in Terai Arc Landscape (TAL) of India
    Mammalian Biology, 2011
    Co-Authors: Reeta Sharma, Surendra Prakash Goyal, Heiko Stuckas, Ranjana Bhaskar, Imran Khan, Ralph Tiedemann
    Abstract:

    Abstract We analyzed mtDNA polymorphisms (a total of 741 bp from a part of conserved control region, ND5, ND2, Cyt b and 12S) in 91 scats and 12 tissue samples of Bengal Tiger ( Panthera tigris tigris ) populations across Terai Arc Landscape (TAL) located at the foothills of Himalayas in North Western India, Buxa Tiger Reserve (BTR), and North East India. In TAL and BTR, we found a specific haplotype at high frequency, which was absent elsewhere, indicating a genetically distinct population in these regions. Within the TAL region, there is some evidence for genetic isolation of the Tiger populations west of river Ganges, i.e., in the western part of Rajaji National Park (RNP). Although the river itself might not constitute a significant barrier for Tigers, recent human-induced changes in habitat and degradation of the Motichur-Chilla Corridor connecting the two sides of the Tiger habitat of RNP might effectively prevent genetic exchange. A cohesive population is observed for the rest of the TAL. Even the more eastern BTR belongs genetically to this unit, despite the present lack of a migration corridor between BTR and TAL. In spite of a close geographic proximity, Chitwan (Nepal) constitutes a Tiger population genetically different from TAL. Moreover, it is observed that the North East India Tiger populations are genetically different from TAL and BTR, as well as from the other Bengal Tiger populations in India.

Jouni Aspi - One of the best experts on this subject based on the ideXlab platform.

  • fine scale population genetic structure of the Bengal Tiger panthera tigris tigris in a human dominated western terai arc landscape india
    PLOS ONE, 2017
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Puneet Pandey, Reeta Sharma, Randeep Singh, Manoj Agrawal, Surendra Prakash Goyal
    Abstract:

    Despite massive global conservation strategies, Tiger populations continued to decline until recently, mainly due to habitat loss, human-animal conflicts, and poaching. These factors are known to affect the genetic characteristics of Tiger populations and decrease local effective population sizes. The Terai Arc Landscape (TAL) at the foothills of the Himalaya is one of the 42 source sites of Tigers around the globe. Therefore, information on how landscape features and anthropogenic factors affect the fine-scale spatial genetic structure and variation of Tigers in TAL is needed to develop proper management strategies for achieving long-term conservation goals. We document, for the first time, the genetic characteristics of this Tiger population by genotyping 71 Tiger samples using 13 microsatellite markers from the western region of TAL (WTAL) of 1800 km2. Specifically, we aimed to estimate the genetic variability, population structure, and gene flow. The microsatellite markers indicated that the levels of allelic diversity (MNA = 6.6) and genetic variation (Ho = 0.50, HE = 0.64) were slightly lower than those reported previously in other Bengal Tiger populations. We observed moderate gene flow and significant genetic differentiation (FST= 0.060) and identified the presence of cryptic genetic structure using Bayesian and non-Bayesian approaches. There was low and significantly asymmetric migration between the two main subpopulations of the Rajaji Tiger Reserve and the Corbett Tiger Reserve in WTAL. Sibship relationships indicated that the functionality of the corridor between these subpopulations may be retained if the quality of the habitat does not deteriorate. However, we found that gene flow is not adequate in view of changing land use matrices. We discuss the need to maintain connectivity by implementing the measures that have been suggested previously to minimize the level of human disturbance, including relocation of villages and industries, prevention of encroachment, and banning sand and boulder mining in the corridors.

  • Comparison of ecological exchangeability between Sundarbans and mainland Bengal Tiger landscape in India.
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    Comparison of ecological exchangeability between Sundarbans and mainland Bengal Tiger landscape in India.

  • Allele size range, Number of alleles (Na) and genotyping error rates (ADO = allelic dropout, FA = False allele) at nine microsatellite loci with field collected scat (n = 10; for Kanha Tiger reserve), tissue samples (n = 10; from Corbett Tiger reserv
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    Allele size range, Number of alleles (Na) and genotyping error rates (ADO = allelic dropout, FA = False allele) at nine microsatellite loci with field collected scat (n = 10; for Kanha Tiger reserve), tissue samples (n = 10; from Corbett Tiger reserve) and scat & hair samples from Sundarbans (n = 8) of wild Bengal Tiger.

  • Median-joining network created from four mtDNA genes (cytb, ND2, ND5 and ND6) (in total, 2600 bp) depicting genetic relationship between all haplotypes found in Tigers.
    2015
    Co-Authors: Sujeet Kumar Singh, Sudhanshu Mishra, Jouni Aspi, Laura Kvist, Parag Nigam, Puneet Pandey, Reeta Sharma, Surendra Prakash Goyal
    Abstract:

    (a) haplotypes found in Sundarbans Tigers (in black) and all other six Tiger subspecies (in yellow and green color, from Luo et al. 2004) [2], (b) all haplotypes found in Bengal Tiger populations from this study and Mondol et al. [22]. Pink: North India, Yellow: Central India, Blue: South India, and Green: Sundarbans. The sizes of the circles are proportional to the haplotype frequencies.

  • Panel of polymorphic heterologous microsatellite loci to genotype critically endangered Bengal Tiger: a pilot study
    SpringerPlus, 2014
    Co-Authors: Sudhanshu Mishra, Sujeet Kumar Singh, Jouni Aspi, Ashok Kumar Munjal, Surendra Prakash Goyal
    Abstract:

    In India, six landscapes and source populations that are important for long-term conservation of Bengal Tigers ( Panthera tigris tigris ) have been identified. Except for a few studies, nothing is known regarding the genetic structure and extent of gene flow among most of the Tiger populations across India as the majority of them are small, fragmented and isolated. Thus, individual-based relationships are required to understand the species ecology and biology for planning effective conservation and genetics-based individual identification has been widely used. But this needs screening and describing characteristics of microsatellite loci from DNA from good-quality sources so that the required number of loci can be selected and the genotyping error rate minimized. In the studies so far conducted on the Bengal Tiger, a very small number of loci (n = 35) have been tested with high-quality source of DNA, and information on locus-specific characteristics is lacking. The use of such characteristics has been strongly recommended in the literature to minimize the error rate and by the International Society for Forensic Genetics (ISFG) for forensic purposes. Therefore, we describe for the first time locus-specific genetic and genotyping profile characteristics, crucial for population genetic studies, using high-quality source of DNA of the Bengal Tiger. We screened 39 heterologous microsatellite loci (Sumatran Tiger, domestic cat, Asiatic lion and snow leopard) in captive individuals (n = 8), of which 21 loci are being reported for the first time in the Bengal Tiger, providing an additional choice for selection. The mean relatedness coefficient (R = −0.143) indicates that the selected Tigers were unrelated. Thirty-four loci were polymorphic, with the number of alleles ranging from 2 to 7 per locus, and the remaining five loci were monomorphic. Based on the PIC values (> 0.500), and other characteristics, we suggest that 16 loci (3 to 7 alleles) be used for genetic and forensic study purposes. The probabilities of matching genotypes of unrelated individuals (3.692 × 10^-19) and siblings (4.003 × 10^-6) are within the values needed for undertaking studies in population genetics, relatedness, sociobiology and forensics.

Laura Kvist - One of the best experts on this subject based on the ideXlab platform.