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R. S. Jones - One of the best experts on this subject based on the ideXlab platform.
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Physiologic study of Bile salt and lipid secretion in rats after liver transplantation.
Annals of surgery, 1993Co-Authors: J. A. Pilcher, R. S. JonesAbstract:OBJECTIVE: This experiment determined the effects of liver transplantation on Bile salt kinetics and biliary lipid secretion. SUMMARY BACKGROUND DATA: Depression of Bile secretion in the three main components and Bile cholesterol supersaturation with a high incidence of cholesterol stone formation have been documented in patients with end stage liver diseases after transplantation. However, physiologic changes of Bile salt and lipid secretion in recipients remain unclear. METHODS: The study was done using a rat liver transplant model with 1 and 6 hours of donor liver cold preservation in saline. A chronic common Bile Duct Fistula and a duodenal cannula were established for Bile collection and the sample biochemical study for 6 days. RESULTS: Altered Bile salt kinetics in liver grafted rats included depressed Bile flow for 1-3 hours after the revascularization, decreased Bile salt concentration and outputs, reduced Bile salt basal synthesis rate, and reduced Bile salt pool size during the early postoperative period. Phospholipid concentration and secretion rate depressed for 1-3 days without altered cholesterol level. The uncoupling change of the cholesterol and the other Bile components resulted in an increased lithogenic indices in Bile on days 2 and 3. CONCLUSION: Alterations of Bile composition and Bile salt kinetics occur in liver transplanted rats. The changes may result from injuries of the liver microcirculation and parenchymal cells caused by cold preservation. The study of Bile secretion is helpful for evaluation of the initial graft function. The changed ratio of three Bile components may be important for cholesterol stone formation in the liver transplant recipient.
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Bile secretion and liver regeneration in partially hepatectomized rats.
Annals of surgery, 1993Co-Authors: Lynn K. Rosenlof, R. S. JonesAbstract:OBJECTIVE: The purpose of this research was to study the correlation between Bile secretion and the liver regeneration in the partially hepatectomized rat. SUMMARY BACKGROUND DATA: Significant alteration in Bile formation and secretion is expected in the liver after hepatectomy. There is scant literature, however, about the effects of liver regeneration in Bile secretion. METHODS: The work was done in rats with 50% hepatectomy, 75% hepatectomy, and sham operation as the control. A chronic common Bile Duct Fistula and a duodenal cannula were established for Bile collection and the sample analysis on days 1, 3, 5, 7, and 9. RESULTS: With size reduced in the liver after 50% and 75% hepatectomy, the total Bile volume decreased 45.9% and 51.5%, Bile salt independent flow decreased 59.3% and 64.9%, Bile salt secretion rate decreased 36.1% and 43.4%, Bile salt basal synthesis rate decreased 52.3% and 56.4%, phospholipid secretion rate decreased 52.6% and 68.0%, and cholesterol secretion rate decreased 54.3% and 72.4% from control on day 1, respectively. All changes returned to the control level in 3 to 9 days with accompanying increasing liver size during regeneration. CONCLUSION: Alterations of total Bile flow, Bile salt independent flow, Bile salt secretion rate, Bile salt basal synthesis rate, and biliary lipid secretion after partial hepatectomy correlate with the liver regeneration rate in rats. Partial hepatectomy reduces the Bile salt independent fraction calculated as per 100 g body weight rather than the dependent fraction. The study of Bile salt and biliary lipid secretion is a useful method for monitoring synthetic function in liver regeneration in vivo.
Yasushi Sunami - One of the best experts on this subject based on the ideXlab platform.
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Tauroursodeoxycholate and taurochenodeoxycholate stabilize Bile lipid metastability through different mechanisms: relation to phospholipid fatty acid composition
Hepatology Research, 1997Co-Authors: Susumu Tazuma, Hiroaki Miyake, Hiroyuki Miura, Tsuyoshi Kajihara, Yoshihiro Hattori, Denya Tsuchimoto, Tomoji Nishioka, Hideyuki Hyogo, Shigeyuki Yasumiba, Yasushi SunamiAbstract:Abstract Lecithin–cholesterol vesicles are present in Bile and play a role in cholesterol metastability. Cholesterol holding capacity of vesicles is regulated by lecithin hydrophobicity. In the present study, the effect of therapeutic Bile salts on biliary lipid secretion rate and phospholipid fatty acid composition was evaluated in Bile Duct-Fistula rats to determine whether Bile salts modulate biliary phospholipid species to, thereby, regulate a physico-chemical metastability of Bile cholesterol. Rats were depleted with Bile salt pool by overnight biliary diversion and reinfused intravenously with sodium taurocholate (TC) at a constant rate (200 nmol/min per 100 g b.w.) for 3 h, followed by infusion of either sodium taurochenodeoxycholate (TCDC) or tauroursodeoxycholate (TUDC) at a compatible rate. TCDC-infusion increased the biliary secretion rate of cholesterol and phospholipids with a decrease in molar ratio of cholesterol to phospholipid ( C / P ) when compared to values under TC-infusion, and this was associated with an increase in the molar ratio of phospholipid saturated to unsaturated fatty acids ( S / U ). In contrast, TUDC-infusion decreased such a biliary lipid secretion rate with an increase in C / P , and this was associated with a decrease in a molar ratio of arachidonate in phospholipids. These findings indicate that TCDC enhances a cholesterol packing density of biliary particulate species by decreasing C / P ratio and increasing S / U , whereas TUDC improves Bile lithogenecity by decreasing biliary cholesterol and phospholipid secretion rates and reducing Bile arachidonyl phospholipids.
J. A. Pilcher - One of the best experts on this subject based on the ideXlab platform.
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Physiologic study of Bile salt and lipid secretion in rats after liver transplantation.
Annals of surgery, 1993Co-Authors: J. A. Pilcher, R. S. JonesAbstract:OBJECTIVE: This experiment determined the effects of liver transplantation on Bile salt kinetics and biliary lipid secretion. SUMMARY BACKGROUND DATA: Depression of Bile secretion in the three main components and Bile cholesterol supersaturation with a high incidence of cholesterol stone formation have been documented in patients with end stage liver diseases after transplantation. However, physiologic changes of Bile salt and lipid secretion in recipients remain unclear. METHODS: The study was done using a rat liver transplant model with 1 and 6 hours of donor liver cold preservation in saline. A chronic common Bile Duct Fistula and a duodenal cannula were established for Bile collection and the sample biochemical study for 6 days. RESULTS: Altered Bile salt kinetics in liver grafted rats included depressed Bile flow for 1-3 hours after the revascularization, decreased Bile salt concentration and outputs, reduced Bile salt basal synthesis rate, and reduced Bile salt pool size during the early postoperative period. Phospholipid concentration and secretion rate depressed for 1-3 days without altered cholesterol level. The uncoupling change of the cholesterol and the other Bile components resulted in an increased lithogenic indices in Bile on days 2 and 3. CONCLUSION: Alterations of Bile composition and Bile salt kinetics occur in liver transplanted rats. The changes may result from injuries of the liver microcirculation and parenchymal cells caused by cold preservation. The study of Bile secretion is helpful for evaluation of the initial graft function. The changed ratio of three Bile components may be important for cholesterol stone formation in the liver transplant recipient.
Jun Zhu - One of the best experts on this subject based on the ideXlab platform.
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The imaging features and value of multi-slice spiral CT in the diagnosis of internal biliary Fistulas
Chinese Journal of Hepatobiliary Surgery, 2018Co-Authors: Junqing Wang, Lei Zhang, Zhong Ding, Jun ZhuAbstract:Objective To analyze the typical imaging features of various internal biliary Fistulas by multislice spiral CT (MSCT), and the diagnostic value. Methods the data of 56 cases of internal biliary Fistula in Wuxi No. 2 Affiliated Hospital of Nanjing Medical University from January 2011 to March 2018 were analyzed retrospectively. All cases were examined by MSCT plain scan and enhanced examination. The imaging features of various biliary Fistula were analyzed and studied. Results Among 56 cases of biliary Fistula, 39 cases were gallbladder duodenum Fistula, and the diagnostic sensitivity was 87.2% (34/39). The total coincidence rate was 85.7% (48/56). 21 cases were Fistula formation, and 11 cases of narrow neck syndrome. Gallbladder Bile Duct Fistula, liver-gallbladder Fistula, Bile Duct and duodenal Fistula of 6 cases were showed. In 4 cases of complex gallbladder Fistulas, 3 cases showed Fistula clearly including 2 cases of Fistula and a "clover" sign. In the indirect CT signs of 56 cases of internal biliary Fistula, the common imaging features: atrophy of gallbladder or incarceration, stone incarceration of biliary tract, gallbladder or choledochal Duct and cavity viscera. Conclusion MSCT scan can not only distinguish the type of biliary Fistula and the structure of Fistula, but also display the Fistula, shape, atrophy of gallbladder, gallbladder and Bile Duct gas, stone and surrounding conditions, which have important guiding significance for the formulation of the operation scheme. Key words: Internal biliary Fistula; Gallstone ileus; Multi-slice spiral CT(MSCT); Diagnosis
Robert E. Carraway - One of the best experts on this subject based on the ideXlab platform.
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Neurotensin elevates hepatic Bile acid secretion in chickens by a mechanism requiring an intact enterohepatic circulation
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 2000Co-Authors: Xianyong Gui, Teresa F. Degolier, Gary E. Duke, Robert E. CarrawayAbstract:Abstract Neurotensin (NT), given intravenously at 10–50 pmol/kg per min to anesthetized female chickens equipped with a Bile Duct Fistula, dose-dependently elevated hepatic Bile flow and Bile acid output but only when the enterohepatic circulation was maintained by returning the Bile to the intestinal lumen. Infusion of NT at 10 and 50 pmol/kg per min increased the average hepatic Bile acid output over a 30-min period to 138±11 and 188±13% of control, respectively. During infusion of NT, plasma levels of immunoreactive NT (iNT) increased in time from the basal level (14±1.3 pM) to reach steady state at 30 min. There was a near linear relationship between the dose of NT infused and the increment in plasma iNT. In addition, infusion of NT at 40 pmol/kg min gave a plasma level of iNT (≅88 pM) which was within the range of those observed during duodenal perfusion with lipid (54–300 pM) and near to that measured in hepatic portal blood from fed animals (52±5 pM). Perfusion of duodenum with lipid released endogenous NT and increased the rate of hepatic Bile flow. When NT antagonist SR48692 was given, Bile flow rate decreased to the basal level. These results suggest that intestinal NT, released by lipid, may participate in the regulation of hepatic Bile acid output by a mechanism requiring an intact enterohepatic circulation.