The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Keikhosro Karimi - One of the best experts on this subject based on the ideXlab platform.
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synergy of municipal solid waste co processing with lignocellulosic waste for improved Biobutanol Production
Waste Management, 2020Co-Authors: Sara Farmanbordar, Hamid Amiri, Keikhosro KarimiAbstract:Abstract Co-processing of lignocellulosic wastes, e.g., garden and paper wastes, and the organic matters fraction of municipal solid waste (OMSW) in an integrated bioprocess is a possible approach to realize the potential of wastes for Biobutanol Production. Dilute acid pretreatment is a multi-functional stage for breaking the recalcitrant lignocellulose’s structure, hydrolyzing hemicellulose, and hydrolyzing/solubilizing starch, leading to a pretreated solid and a rich hydrolysate. In this study, dilute-acid pretreatment of the combination of wastepaper and OMSW, composite I, as well as garden waste and OMSW, composite II, at severe conditions resulted in “pretreatment hydrolysates” containing 33.7 and 19.4 g/L sugar along with 18.9 and 33.2 g/L soluble starch, respectively. In addition, the hydrolysis of solid remained after the pretreatment of composite I and II resulted in “enzymatic hydrolysates” comprising 19.4 and 33 g/L sugar, respectively. The fermentation of the pretreatment hydrolysates and enzymatic hydrolysates resulted in 3.5 and 6.4 g/L ABE from composite I and 15 and 5.2 g/L ABE from composite II, respectively. In this process, 148 and 173 g ABE (60 and 100 g gasoline equivalent/kg) was obtained from each kg composite I and composite II, respectively, where co-processing of OMSW with lignocellulosic wastes resulted in 10 and 49% higher ABE than that produced from the individual substrates.
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Biobutanol Production from municipal solid waste technical and economic analysis
Bioresource Technology, 2020Co-Authors: Parisa Nazemi Ashani, Marzieh Shafiei, Keikhosro KarimiAbstract:Novel processes for the Production of acetone-butanol-ethanol (ABE) from municipal solid waste (MSW) were developed and simulated using Aspen Plus®. In scenario 1, a conventional distillation system was used, while a gas stripping system was coupled with a fermenter in scenario 2. In scenario 3, pervaporation (PV) and gas stripping systems right after the fermentation reactor were applied. Gas stripping increased the total ABE produced while the addition of the PV module decreased the number of distillation columns from 6 to 2 as well as created 6.4% increments in the amount of butanol in comparison with scenario 1. Economical evaluation resulted in having payout periods of 15.9, 4.4, and 2.9 years for scenarios 1 to 3, respectively. These results show that using MSW as an inexpensive sugar-rich feedstock together with gas stripping PV system is a promising solution to overcome the major obstacles in the way of the ABE Production.
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efficient Biobutanol Production from potato peel wastes by separate and simultaneous inhibitors removal and pretreatment
Renewable Energy, 2020Co-Authors: Amirmohammad Abedini, Hamid Amiri, Keikhosro KarimiAbstract:Abstract Potato peel waste (PPW) is a carbohydrate-rich waste from potato industries, which is an environmental threat worldwide. In this study, it was evaluated for Biobutanol Production via acetone-butanol-ethanol fermentation by Clostridium acetobutylicum. The results showed that PPW contained a considerable amount of glycoalkaloids, severe inhibitors for the bacterium. Thus, three processes, i.e., dilute acid pretreatment (Process I), the inhibitors extraction followed by dilute acid hydrolysis (Process II) and ethanol organosolv pretreatment (Process III), were employed before hydrolysis and fermentation to produce ABE. The extraction of glycoalkaloids with ethanol, dilute acid hydrolysis at 180 °C for 60 min, and enzymatic hydrolysis led to a hydrolysate with 36 g/L glucose, which was successfully fermented to 11.6 g/L ABE. In process II, the organosolv pretreatment led to the removal of the major fraction of inhibitors, in the range of 77–88% of glycoalkaloids. The enzymatic hydrolysis of PPW pretreated with 75% ethanol at 180 °C for 60 min resulted in a fermentable hydrolysate with 38 g/L glucose. The fermentation of overall hydrolysate resulted in a high ABE concentration of 24.8 g/L, indicating that PPW is an appropriate substrate for butanol Production after the removal of its bacterial inhibitors.
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Biobutanol Production from corn stover in the us
Industrial Crops and Products, 2019Co-Authors: Masih Karimi Alavijeh, Keikhosro KarimiAbstract:Abstract On account of complexities associated with commercial Production of second-generation biofuels, comprehensive examinations are required to enhance the economic feasibility of Biobutanol Production from cellulosic compounds. As the most abundant crop residue in the US, corn stover is a readily available feedstock for biorefineries. However, there are few studies to evaluate the corn stover application in the large-scale Biobutanol Production. In this regard, in order to estimate the amount of collectible corn stover special to Biobutanol Production, a quantitate model was presented. In this model, the residue potential quantity, job creation, butanol blends potential quantity, greenhouse gases (GHG) emissions and social costs, forecasting future trends, economic evaluation, and risk analysis are considered. It was estimated that a total quantity of 84 million tonnes (Mt) of stover for the Production of about 10.48 gigaliters (Gl) of Biobutanol can potentially be collected. Around 87.4% of the total collectible residues is produced in the Midwest. A total of 81,000 jobs can be generated and Iowa can potentially create the most jobs. Furthermore, around 9.5 Gl of gasoline can be saved and about 64.6 Gl of butanol-gasoline blend (Bu16) can potentially be produced from corn residues in the US, which is equivalent to 11.8% of total domestic gasoline consumption. The average minimum selling price (MSP) in the Midwest was $5.76 and $4.05 per gallon depending on the feedstock price, and Illinois, Indiana, Iowa, Minnesota, Nebraska, and South Dakota were predicted to be very attractive in terms of lower selling prices.
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pretreatment and hydrolysis of lignocellulosic wastes for butanol Production challenges and perspectives
Bioresource Technology, 2018Co-Authors: Hamid Amiri, Keikhosro KarimiAbstract:Butanol is acknowledged as a drop-in biofuel that can be used in the existing transportation infrastructure, addressing the needs for sustainable liquid fuel. However, before becoming a thoughtful alternative for fossil fuel, butanol should be produced efficiently from a widely-available, renewable, and cost-effective source. In this regard, lignocellulosic materials, the main component of organic wastes from agriculture, forestry, municipalities, and even industries seems to be the most promising source. The butanol-producing bacteria, i.e., Clostridia sp., can uptake a wide range of hexoses, pentoses, and oligomers obtained from hydrolysis of cellulose and hemicellulose content of lignocelluloses. The present work is dedicated to reviewing different processes containing pretreatment and hydrolysis of hemicellulose and cellulose developed for preparing fermentable hydrolysates for Biobutanol Production.
Suraini Abd-aziz - One of the best experts on this subject based on the ideXlab platform.
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Improved Biobutanol Production in 2-L Simultaneous Saccharification and Fermentation with Delayed Yeast Extract Feeding and in-situ Recovery
Scientific Reports, 2019Co-Authors: Muhammad Siddiq Mohamed Salleh, Mohamad Faizal Ibrahim, Ahmad Muhaimin Roslan, Suraini Abd-azizAbstract:Simultaneous saccharification and fermentation (SSF) with delayed yeast extract feeding (DYEF) was conducted in a 2-L bioreactor equipped with in-situ recovery using a gas stripping in order to enhance Biobutanol Production from lignocellulosic biomass of oil palm empty fruit bunch (OPEFB). This study showed that 2.88 g/L of Biobutanol has been produced from SSF with a similar yield of 0.23 g/g as compared to separate hydrolysis and fermentation (SHF). An increase of 42% of Biobutanol concentration was observed when DYEF was introduced in the SSF at 39 h of fermentation operation. Biobutanol Production was further enhanced up to 11% with a total improvement of 72% when in-situ recovery using a gas stripping was implemented to reduce the solvents inhibition in the bioreactor. In overall, DYEF and in-situ recovery were able to enhance Biobutanol Production in SSF.
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Optimisation of Simultaneous Saccharification and Fermentation (SSF) for Biobutanol Production Using Pretreated Oil Palm Empty Fruit Bunch
MDPI AG, 2018Co-Authors: Nur Atheera Aiza Md Razali, Mohamad Faizal Ibrahim, Ezyana Kamal Bahrin, Suraini Abd-azizAbstract:This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for Biobutanol Production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of Biobutanol concentration and 0.10 g/g of Biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% (w/v). This optimisation study resulted in 55.95% increment (2.14 fold) of Biobutanol concentration equivalent to 3.97 g/L and Biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of Biobutanol Production, especially in consolidated bioprocessing technology
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Statistical optimization of Biobutanol Production from oil palm decanter cake hydrolysate by clostridium acetobutylicum ATCC 824
BioResources, 2013Co-Authors: Mohamad Nafis Abdul Razak, Phang Lai Yee, Mohamad Faizal Ibrahim, Mohd Ali Hassan, Suraini Abd-azizAbstract:Oil palm decanter cake (OPDC) is a potential lignocellulosic biomass for the biofuel industry. The fermentation conditions for Biobutanol Production using glucose from OPDC hydrolysate by Clostridium acetobutylicum ATCC 824 were optimized via response surface methodology (RSM). An analysis of variance (ANOVA) using 2-level factorial was successfully screened. Three significant variables were found to influence the Biobutanol yield: glucose concentrations in the OPDC hydrolysate, inoculum sizes, and initial pH. The concentration of yeast extract, however, showed an insignificant effect in this study. The batch fermentation was analyzed using central composite design (CCD), and it yielded significant variables and the predicted optimum conditions were 70.00 g/L of OPDC hydrolysate, 16.20% of inoculum size, and an initial pH of 5.20. The predicted yield of Biobutanol was 0.09 g/g using 70.00 g/L of glucose. The optimum conditions were validated, and the actual Biobutanol yield was 0.11 g/g with 54.86 g/L of glucose consumption. The Biobutanol Production using synthetic glucose was 15.38% higher when compared to OPDC hydrolysate, but the utilization of OPDC as alternative substrate was still comparable with other findings.
Mohamad Faizal Ibrahim - One of the best experts on this subject based on the ideXlab platform.
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Improved Biobutanol Production in 2-L Simultaneous Saccharification and Fermentation with Delayed Yeast Extract Feeding and in-situ Recovery
Scientific Reports, 2019Co-Authors: Muhammad Siddiq Mohamed Salleh, Mohamad Faizal Ibrahim, Ahmad Muhaimin Roslan, Suraini Abd-azizAbstract:Simultaneous saccharification and fermentation (SSF) with delayed yeast extract feeding (DYEF) was conducted in a 2-L bioreactor equipped with in-situ recovery using a gas stripping in order to enhance Biobutanol Production from lignocellulosic biomass of oil palm empty fruit bunch (OPEFB). This study showed that 2.88 g/L of Biobutanol has been produced from SSF with a similar yield of 0.23 g/g as compared to separate hydrolysis and fermentation (SHF). An increase of 42% of Biobutanol concentration was observed when DYEF was introduced in the SSF at 39 h of fermentation operation. Biobutanol Production was further enhanced up to 11% with a total improvement of 72% when in-situ recovery using a gas stripping was implemented to reduce the solvents inhibition in the bioreactor. In overall, DYEF and in-situ recovery were able to enhance Biobutanol Production in SSF.
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advanced bioprocessing strategies for Biobutanol Production from biomass
Renewable & Sustainable Energy Reviews, 2018Co-Authors: Mohamad Faizal Ibrahim, Seung Wook Kim, Suraini AbdazizAbstract:Abstract Biobutanol Production as a renewable and sustainable biofuel is gaining interest in replacing non-renewable and depleting petrol fuel. In addition to its superior characteristics over bioethanol as a fuel in transportation, butanol is also in demand as a chemical substituent for various industries. Therefore, many researchers have investigated to produce Biobutanol at a low cost by considering suitable feedstock material and bioprocessing technologies. Renewable materials such as starch, lignocellulosic, and algal biomass are some of the common feedstock utilized for Biobutanol Production, and each of them has its own advantages. The limitations of the conventional batch fermentation have been overcome by several fermentation operations and integrated bioprocessing technologies, which had improved Biobutanol Production efficiency. The success of fermenting biomass into Biobutanol relies on the suitability of fermentation operation to correlate with the microbial behavior together with bioprocessing strategies in order to improve the whole process to be viable for industrial scale. Therefore, this review discusses the bioprocessing technologies and suitable strategies that have endeavored to enhance Biobutanol Production from renewable biomass.
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Optimisation of Simultaneous Saccharification and Fermentation (SSF) for Biobutanol Production Using Pretreated Oil Palm Empty Fruit Bunch
MDPI AG, 2018Co-Authors: Nur Atheera Aiza Md Razali, Mohamad Faizal Ibrahim, Ezyana Kamal Bahrin, Suraini Abd-azizAbstract:This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for Biobutanol Production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of Biobutanol concentration and 0.10 g/g of Biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% (w/v). This optimisation study resulted in 55.95% increment (2.14 fold) of Biobutanol concentration equivalent to 3.97 g/L and Biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of Biobutanol Production, especially in consolidated bioprocessing technology
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Statistical optimization of Biobutanol Production from oil palm decanter cake hydrolysate by clostridium acetobutylicum ATCC 824
BioResources, 2013Co-Authors: Mohamad Nafis Abdul Razak, Phang Lai Yee, Mohamad Faizal Ibrahim, Mohd Ali Hassan, Suraini Abd-azizAbstract:Oil palm decanter cake (OPDC) is a potential lignocellulosic biomass for the biofuel industry. The fermentation conditions for Biobutanol Production using glucose from OPDC hydrolysate by Clostridium acetobutylicum ATCC 824 were optimized via response surface methodology (RSM). An analysis of variance (ANOVA) using 2-level factorial was successfully screened. Three significant variables were found to influence the Biobutanol yield: glucose concentrations in the OPDC hydrolysate, inoculum sizes, and initial pH. The concentration of yeast extract, however, showed an insignificant effect in this study. The batch fermentation was analyzed using central composite design (CCD), and it yielded significant variables and the predicted optimum conditions were 70.00 g/L of OPDC hydrolysate, 16.20% of inoculum size, and an initial pH of 5.20. The predicted yield of Biobutanol was 0.09 g/g using 70.00 g/L of glucose. The optimum conditions were validated, and the actual Biobutanol yield was 0.11 g/g with 54.86 g/L of glucose consumption. The Biobutanol Production using synthetic glucose was 15.38% higher when compared to OPDC hydrolysate, but the utilization of OPDC as alternative substrate was still comparable with other findings.
Duujong Lee - One of the best experts on this subject based on the ideXlab platform.
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Biobutanol Production from lignocellulosic biomass using immobilized clostridium acetobutylicum
Applied Energy, 2020Co-Authors: Tsung Yu Tsai, Jo Shu Chang, Dillirani Nagarajan, Duujong Lee, Cheng Di DongAbstract:Abstract Biobutanol produced by acetone-Biobutanol-ethanol (ABE) fermentation process has been revisited in the light of its use as “drop in” liquid biofuel to be blended with gasoline. In this study, renewable feedstock like rice straw, sugarcane bagasse and microalgal hydrolysate were used in ABE fermentation via separate hydrolysis and fermentation. Clostridium acetobutylicum ATCC 824 was used as the fermenting organism. Alkali pretreatment followed by enzymatic hydrolysis was used for rice straw and sugarcane bagasse. In batch fermentation, a Biobutanol titer and yield of 9.10 g/L and 0.42 mol/mol glucose (0.17 g Biobutanol/g glucose), respectively was obtained from rice straw, while sugarcane bagasse achieved a Biobutanol titer and yield of 8.40 g/L and 0.40 mol/mol glucose (0.16 g Biobutanol/g glucose), respectively. Higher microalgal biomass loading with 3% acid pretreatment severely inhibited fermentation performance. Unhydrolyzed microalgal biomass at a loading of 180 g/L in ABE fermentation resulted in 4.32 g/L Biobutanol and 0.09 g Biobutanol/g microalgae as yield. C. acetobutylicum was immobilized in polyvinyl alcohol (PVA) for improving the cell loading in fermentation and protect the cells from Biobutanol toxicity. With rice straw hydrolysate as a feedstock and in the absence of yeast extract, a Biobutanol titer, yield and productivity of 13.80 g/L, 0.90 g/L/h, and 0.58 mol Biobutanol/mol glucose (0.23 g Biobutanol/g glucose), respectively were obtained. Hence, rice straw is a potential feedstock for Biobutanol Production for fuel use.
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current advances on fermentative Biobutanol Production using third generation feedstock
Biotechnology Advances, 2017Co-Authors: Yue Wang, Hongwei Yen, Dillirani Nagarajan, Nanqi Ren, Duujong Lee, Akihiko Kondo, Jo Shu ChangAbstract:Biobutanol is gaining more attention as a potential alternative to ethanol, and the demand for fermentative Biobutanol Production has renewed interest. The main challenge faced in Biobutanol Production is the availability of feedstock. Using conventional agricultural biomass as feedstock is controversial and less efficient, while microalgae, the third generation feedstock, are considered promising feedstock for Biobutanol Production due to their high growth rate and high carbohydrates content. This review is primarily focused on Biobutanol Production by using carbohydrate-rich microalgal feedstock. Key technologies and challenges involved in producing butanol from microalgae are discussed in detail and future directions are also presented.
Mohd Sahaid Kalil - One of the best experts on this subject based on the ideXlab platform.
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Biobutanol Production by a new aerotolerant strain of clostridium acetobutylicum ym1 under aerobic conditions
Fuel, 2015Co-Authors: Najeeb Kaid Nasser Alshorgani, Mohd Sahaid Kalil, Wan Mohtar Wan Yusoff, Aidil Abdul HamidAbstract:Abstract A newly isolated strain of Clostridium acetobutylicum YM1 has a unique property of producing Biobutanol from glucose under aerobic conditions. This strain exhibited the capability to grow and produce high concentrations of Biobutanol under different concentrations of dissolved oxygen (DO). The growth profile and solvent Production in a 5 L bioreactor were similar under anaerobic and aerobic conditions (100% initial DO saturation), and the final Biobutanol Production was 12.18 and 12.30 g/L, respectively. The addition of reducing chemical agents, without creating anaerobic conditions, to the culture of YM1 enhanced the Production of Biobutanol. Strain YM1 possesses different enzymes that are responsible for oxygen scavenging, such as superoxide dismutase (SOD), catalase and NADH/NADPH oxidases. This study provides a simple operation strategy for more efficient Biobutanol Production by using an aerotolerant strain of C. acetobutylicum YM1 without the need to flush the medium with nitrogen gas to ensure anaerobic conditions.
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Production of butanol by clostridium saccharoperbutylacetonicum n1 4 from palm kernel cake in acetone butanol ethanol fermentation using an empirical model
Bioresource Technology, 2014Co-Authors: Hafiza Shukor, Najeeb Kaid Nasser Alshorgani, Nurina Anuar, Peyman Abdeshahian, Aidil Abdul Hamid, Norliza Abd Rahman, Mohd Sahaid KalilAbstract:Abstract Palm kernel cake (PKC) was used for Biobutanol Production by Clostridium saccharoperbutylacetonicum N1-4 in acetone–butanol–ethanol (ABE) fermentation. PKC was subjected to acid hydrolysis pretreatment and hydrolysates released were detoxified by XAD-4 resin. The effect of pH, temperature and inoculum size on butanol Production was evaluated using an empirical model. Twenty ABE fermentations were run according to an experimental design. Experimental results revealed that XAD-4 resin removed 50% furfural and 77.42% hydroxymethyl furfural. The analysis of the empirical model showed that linear effect of inoculums size with quadratic effect of pH and inoculum size influenced butanol Production at 99% probability level (P
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Biobutanol Production from palm kernel cake pkc using clostridium saccharoperbutylacetonicum n1 4 in batch culture fermentation
Bioresources, 2014Co-Authors: Hafiza Shukor, Najeeb Kaid Nasser Alshorgani, Nurina Anuar, Peyman Abdeshahian, Aidil Abdul Hamid, Norliza Abd Rahman, Mohd Hafez Mohd Isa, Mohd Sahaid KalilAbstract:Palm kernel cake (PKC), a by-product of palm oil industry, contains glucose and mannose as hexose sugars. This study was performed to determine the feasibility of using PKC as a lignocellulosic substrate for Biobutanol Production by Clostridium saccharoperbutylacetonicum N1-4 in an acetone-butanol-ethanol (ABE) fermentation process. Moreover, the effect of tryptone-yeast extract-acetate (TYA) medium and P2 medium on Biobutanol Production was evaluated. Experimental results showed that butanol Production of 3.05 g/L was obtained using mannose sugar, which was comparable to 3.61 g/L butanol Production measured using glucose. Moreover, the maximum Production of Biobutanol (0.38 g/L) was obtained at a PKC concentration of 30%, indicating the possibility of PKC utilization in butanol Production. ABE fermentation of PKC using distilled water, TYA medium, and P2 medium showed that the highest butanol Production (0.26 g/L) with ABE Production of 0.38 g/L was obtained when ABE fermentation was conducted in P2 medium.
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pre optimization of medium for Biobutanol Production by a new isolate of solvent producing clostridium
Bioresources, 2013Co-Authors: Najeeb Kaid Nasser Alshorgani, Aidil Abdul Hamid, Wan Mohtar Wan Yusoff, Mohd Sahaid KalilAbstract:A Plackett-Burman design was used to pre-optimize the medium composition for Biobutanol Production using a unique isolate of solvent-producing Clostridium YM1. Various nutrient factors affecting Biobutanol Production were screened using the Plackett-Burman design. These factors included: glucose, tryptone, yeast extract, peptone, ammonium acetate, KH2PO4, K2HPO4, MgSO4, FeSO4, Na2CO3, and NaCl. The results were analyzed by an analysis of variance (ANOVA), which showed that glucose, tryptone, yeast extract, peptone, K2HPO4, Na2CO3, and MgSO4 had significant effects on Biobutanol Production. However, ammonium acetate, KH2PO4, and FeSO4 had insignificant effects. The established model from the ANOVA analysis had a significant value of Pmodel>F = 0.0245 and an R2 value of 0.999. The estimated maximum Biobutanol Production was 9.01 g/L, whereas the optimized medium produced 10.93 g/L of Biobutanol.