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Li Guo - One of the best experts on this subject based on the ideXlab platform.

  • chromosome scale genome assembly of talaromyces rugulosus w13939 a mycoparasitic fungus and promising Biocontrol Agent
    Molecular Plant-microbe Interactions, 2020
    Co-Authors: Bo Wang, Li Guo
    Abstract:

    Here, we report a chromosome-level genome assembly of Talaromyces rugulosus (syn. Penicillium rugulosum) W13939 (six chromosomes; contig N50: 5.90 Mb), generated using a combination of PacBio long-read and Illumina paired-end data. T. rugulosus is not only a potent enzyme producer, but also a mycoparasite of Aspergillus flavus, which is a notorious plant pathogen and mycotoxin producer, making it a promising Biocontrol Agent. The T. rugulosus genome is rich in genes encoding proteases, carbohydrate-active enzymes, fungal cell wall-degrading enzymes, lectin, and secondary metabolite biosynthetic enzymes, reflecting its mycoparasitic life style and mycotoxigenic capability. This high-quality assembly of the T. rugulosus genome will be a valuable resource to assist us in the understanding of the molecular basis of mycoparasitism and facilitate the agricultural and biotechnological applications of Talaromyces spp.

  • chromosome scale genome assembly of fusarium oxysporum strain fo47 a fungal endophyte and Biocontrol Agent
    Molecular Plant-microbe Interactions, 2020
    Co-Authors: Bo Wang, Yanyan Jia, Quanbin Dong, Christian Steinberg, Claude Alabouvette, Veronique Edelhermann, Corby H Kistler, Li Guo
    Abstract:

    Here, we report a chromosome-level genome assembly of Fusarium oxysporum Fo47 (12 pseudomolecules; contig N50: 4.52 Mb), generated using a combination of PacBio long-read, Illumina paired end, and high-throughput chromosome conformation capture sequencing data. Although F. oxysporum causes vascular wilt to over 100 plant species, the strain Fo47 is classified as an endophyte and is widely used as a Biocontrol Agent for plant disease control. The Fo47 genome carries a single accessory chromosome of 4.23 Mb, compared with the reference genome of F. oxysporum f. sp. lycopersici Fol4287. The high-quality assembly and annotation of the Fo47 genome will be a valuable resource for studying the mechanisms underlying the endophytic interactions between F. oxysporum and plants as well as for deciphering the genome evolution of the F. oxysporum species complex.

  • chromosome scale genome assembly of fusarium oxysporum strain fo47 a fungal endophyte and Biocontrol Agent
    bioRxiv, 2020
    Co-Authors: Bo Wang, Yanyan Jia, Quanbin Dong, Christian Steinberg, Claude Alabouvette, Veronique Edelhermann, Corby H Kistler, Li Guo
    Abstract:

    Abstract Here, we report a chromosome-level genome assembly of Fusarium oxysporum strain Fo47 (12 pseudomolecules; contig N50: 4.52Mb), generated using a combination of PacBio long-read, Illumina pair-ended and Hi-C sequencing data. Although F. oxysporum causes vascular wilt to over 100 plant species, the strain Fo47 is classified as an endophyte and widely used as a Biocontrol Agent for plant disease control. The Fo47 genome carries a single accessory chromosome of 4.23 Mb, compared to the reference genome of F. oxysporum f.sp. lycopersici strain Fol4287. The high-quality assembly and annotation of the Fo47 genome will be a valuable resource for studying the mechanisms underlying the endophytic interactions between F. oxysporum and plants, as well as deciphering the genome evolution of the F. oxysporum species complex.

Bo Wang - One of the best experts on this subject based on the ideXlab platform.

  • chromosome scale genome assembly of talaromyces rugulosus w13939 a mycoparasitic fungus and promising Biocontrol Agent
    Molecular Plant-microbe Interactions, 2020
    Co-Authors: Bo Wang, Li Guo
    Abstract:

    Here, we report a chromosome-level genome assembly of Talaromyces rugulosus (syn. Penicillium rugulosum) W13939 (six chromosomes; contig N50: 5.90 Mb), generated using a combination of PacBio long-read and Illumina paired-end data. T. rugulosus is not only a potent enzyme producer, but also a mycoparasite of Aspergillus flavus, which is a notorious plant pathogen and mycotoxin producer, making it a promising Biocontrol Agent. The T. rugulosus genome is rich in genes encoding proteases, carbohydrate-active enzymes, fungal cell wall-degrading enzymes, lectin, and secondary metabolite biosynthetic enzymes, reflecting its mycoparasitic life style and mycotoxigenic capability. This high-quality assembly of the T. rugulosus genome will be a valuable resource to assist us in the understanding of the molecular basis of mycoparasitism and facilitate the agricultural and biotechnological applications of Talaromyces spp.

  • chromosome scale genome assembly of fusarium oxysporum strain fo47 a fungal endophyte and Biocontrol Agent
    Molecular Plant-microbe Interactions, 2020
    Co-Authors: Bo Wang, Yanyan Jia, Quanbin Dong, Christian Steinberg, Claude Alabouvette, Veronique Edelhermann, Corby H Kistler, Li Guo
    Abstract:

    Here, we report a chromosome-level genome assembly of Fusarium oxysporum Fo47 (12 pseudomolecules; contig N50: 4.52 Mb), generated using a combination of PacBio long-read, Illumina paired end, and high-throughput chromosome conformation capture sequencing data. Although F. oxysporum causes vascular wilt to over 100 plant species, the strain Fo47 is classified as an endophyte and is widely used as a Biocontrol Agent for plant disease control. The Fo47 genome carries a single accessory chromosome of 4.23 Mb, compared with the reference genome of F. oxysporum f. sp. lycopersici Fol4287. The high-quality assembly and annotation of the Fo47 genome will be a valuable resource for studying the mechanisms underlying the endophytic interactions between F. oxysporum and plants as well as for deciphering the genome evolution of the F. oxysporum species complex.

  • chromosome scale genome assembly of fusarium oxysporum strain fo47 a fungal endophyte and Biocontrol Agent
    bioRxiv, 2020
    Co-Authors: Bo Wang, Yanyan Jia, Quanbin Dong, Christian Steinberg, Claude Alabouvette, Veronique Edelhermann, Corby H Kistler, Li Guo
    Abstract:

    Abstract Here, we report a chromosome-level genome assembly of Fusarium oxysporum strain Fo47 (12 pseudomolecules; contig N50: 4.52Mb), generated using a combination of PacBio long-read, Illumina pair-ended and Hi-C sequencing data. Although F. oxysporum causes vascular wilt to over 100 plant species, the strain Fo47 is classified as an endophyte and widely used as a Biocontrol Agent for plant disease control. The Fo47 genome carries a single accessory chromosome of 4.23 Mb, compared to the reference genome of F. oxysporum f.sp. lycopersici strain Fol4287. The high-quality assembly and annotation of the Fo47 genome will be a valuable resource for studying the mechanisms underlying the endophytic interactions between F. oxysporum and plants, as well as deciphering the genome evolution of the F. oxysporum species complex.

Neus Teixidó - One of the best experts on this subject based on the ideXlab platform.

  • formulation development of the Biocontrol Agent bacillus subtilis strain cpa 8 by spray drying
    Journal of Applied Microbiology, 2012
    Co-Authors: V Yanezmendizabal, Josep Usall, Immaculada Viñas, R Torres, C Solsona, Maribel Abadias, Neus Teixidó
    Abstract:

    Aims:  To prepare commercially acceptable formulations of Bacillus subtilis CPA-8 by spray-drying with long storage life and retained efficacy to control peach and nectarine brown rot caused by Monilinia spp. Methods and Results:  CPA-8 24-h- and 72-h-old cultures were spray dried using 10% skimmed milk, 10% skimmed milk plus 10% MgSO4, 10% MgSO4 and 20% MgSO4 as carriers/protectants. All carriers/protectants gave good percentages of powder recovery (28–38%) and moisture content (7–13%). CPA-8 survival varied considerably among spray-dried 24-h- and 72-h-old cultures. Seventy-two hours culture spray dried formulations showed the highest survival (28–32%) with final concentration products of 1·6–3·3 × 109 CFU g−1, while viability of 24-h-old formulations was lower than 1%. Spray-dried 72-h-old formulations were selected to subsequent evaluation. Rehydration of cells with water provided a good recovery of CPA-8 dried cells, similar to other complex rehydration media tested. Spray-dried formulations stored at 4 ± 1 and 20 ± 1°C showed good shelf life during 6 months, and viability was maintained or slightly decreased by 0·2–0·3-log. CPA-8 formulations after 4- and 6 months storage were effective in controlling brown rot caused by Monilinia spp. on nectarines and peaches resulting in a 90–100% reduction in disease incidence. Conclusions:  Stable and effective formulations of Biocontrol Agent B. subtilis CPA-8 could be obtained by spray-drying. Significance and Impact of the Study:  New shelf-stable and effective formulations of a Biocontrol Agent have been obtained by spray-drying to control brown rot on peach.

  • endospore production allows using spray drying as a possible formulation system of the Biocontrol Agent bacillus subtilis cpa 8
    Biotechnology Letters, 2012
    Co-Authors: V Yanezmendizabal, Josep Usall, T P Canamas, Immaculada Viñas, Neus Teixidó
    Abstract:

    The role of endospore production by Bacillus subtilis CPA-8 on survival during spray-drying was investigated by comparison with a non-spore-forming Biocontrol Agent Pantoea agglomerans CPA-2. Endospore formation promoted heat resistance in CPA-8 depending on growth time (72 h cultures were more resistant than 24 h ones). The survival of CPA-8 and CPA-2 after spray-drying was determined after being grown in optimised media for 24 and 72 h. Spray-dried 72 h CPA-8 had the best survival (32%), while CPA-2 viability was less than 2%. CPA-8 survival directly related with its ability to produce endospores. Spray-dried CPA-8 reduced Monilinia fructicola conidia germination similarly to fresh cells, demonstrating that spray-drying did not adversely affect Biocontrol efficacy. Endospore production thus improves CPA-8 resistance to spray-drying. These results can provide a reliable basis for optimising of the spray-drying formulation process for CPA-8 and other microorganisms.

Josep Usall - One of the best experts on this subject based on the ideXlab platform.

  • formulation development of the Biocontrol Agent bacillus subtilis strain cpa 8 by spray drying
    Journal of Applied Microbiology, 2012
    Co-Authors: V Yanezmendizabal, Josep Usall, Immaculada Viñas, R Torres, C Solsona, Maribel Abadias, Neus Teixidó
    Abstract:

    Aims:  To prepare commercially acceptable formulations of Bacillus subtilis CPA-8 by spray-drying with long storage life and retained efficacy to control peach and nectarine brown rot caused by Monilinia spp. Methods and Results:  CPA-8 24-h- and 72-h-old cultures were spray dried using 10% skimmed milk, 10% skimmed milk plus 10% MgSO4, 10% MgSO4 and 20% MgSO4 as carriers/protectants. All carriers/protectants gave good percentages of powder recovery (28–38%) and moisture content (7–13%). CPA-8 survival varied considerably among spray-dried 24-h- and 72-h-old cultures. Seventy-two hours culture spray dried formulations showed the highest survival (28–32%) with final concentration products of 1·6–3·3 × 109 CFU g−1, while viability of 24-h-old formulations was lower than 1%. Spray-dried 72-h-old formulations were selected to subsequent evaluation. Rehydration of cells with water provided a good recovery of CPA-8 dried cells, similar to other complex rehydration media tested. Spray-dried formulations stored at 4 ± 1 and 20 ± 1°C showed good shelf life during 6 months, and viability was maintained or slightly decreased by 0·2–0·3-log. CPA-8 formulations after 4- and 6 months storage were effective in controlling brown rot caused by Monilinia spp. on nectarines and peaches resulting in a 90–100% reduction in disease incidence. Conclusions:  Stable and effective formulations of Biocontrol Agent B. subtilis CPA-8 could be obtained by spray-drying. Significance and Impact of the Study:  New shelf-stable and effective formulations of a Biocontrol Agent have been obtained by spray-drying to control brown rot on peach.

  • endospore production allows using spray drying as a possible formulation system of the Biocontrol Agent bacillus subtilis cpa 8
    Biotechnology Letters, 2012
    Co-Authors: V Yanezmendizabal, Josep Usall, T P Canamas, Immaculada Viñas, Neus Teixidó
    Abstract:

    The role of endospore production by Bacillus subtilis CPA-8 on survival during spray-drying was investigated by comparison with a non-spore-forming Biocontrol Agent Pantoea agglomerans CPA-2. Endospore formation promoted heat resistance in CPA-8 depending on growth time (72 h cultures were more resistant than 24 h ones). The survival of CPA-8 and CPA-2 after spray-drying was determined after being grown in optimised media for 24 and 72 h. Spray-dried 72 h CPA-8 had the best survival (32%), while CPA-2 viability was less than 2%. CPA-8 survival directly related with its ability to produce endospores. Spray-dried CPA-8 reduced Monilinia fructicola conidia germination similarly to fresh cells, demonstrating that spray-drying did not adversely affect Biocontrol efficacy. Endospore production thus improves CPA-8 resistance to spray-drying. These results can provide a reliable basis for optimising of the spray-drying formulation process for CPA-8 and other microorganisms.

  • anti oxidant activity of oranges after infection with the pathogen penicillium digitatum or treatment with the Biocontrol Agent pantoea agglomerans cpa 2
    Biological Control, 2011
    Co-Authors: R Torres, Josep Usall, Maribel Abadias, N Teixido, Christian Larrigaudiere, I Vinas
    Abstract:

    Abstract The suppression or generation of hydrogen peroxide (H 2 O 2 ) in fruit as well as the changes in the superoxide dismutase (SOD) and catalase (CAT) activities due to Penicillium digitatum infection were evaluated on oranges. These biochemical parameters were also determined when oranges were treated with the Biocontrol Agent Pantoea agglomerans CPA-2. After harvest, oranges were divided in two batches of fruit: one was used immediately and another stored at 4 °C for 2 months. In both cases, H 2 O 2 content and changes in enzyme activities were determined at specific time intervals in oranges inoculated with P. digitatum or treated with P. agglomerans . Similar levels of H 2 O 2 were observed during the first 3 days after inoculation in all treatment (control, infected and treated fruit). A short time after, CPA-2 treated fruit showed an accumulation of H 2 O 2 while infected fruit exhibited a sharp decrease in H 2 O 2 levels. An increase of SOD and CAT activities was also observed in CPA-2 treated oranges; in contrast, infected fruit showed a significant reduction of both enzymatic activities. This biochemical approach suggests that P. digitatum suppresses H 2 O 2 production as well as SOD and CAT activities in orange tissue as response to infection process. In contrast, the Biocontrol Agent P. agglomerans CPA-2 triggers H 2 O 2 production and both enzymatic activities probably as a mechanism of action to prevent citrus fruit against future infections of green mold. Our data suggest that P. agglomerans CPA-2 may act as antagonist against green mold not only by competition for nutrients and space but also by inducing oxidative response in orange tissue.

  • production of the Biocontrol Agent pantoea agglomerans strain cpa 2 using commercial products and by products
    Applied Microbiology and Biotechnology, 2001
    Co-Authors: E Costa, Josep Usall, N Teixido, E Atares, I Vinas
    Abstract:

    The aim of this paper was to find the nitrogen and carbon sources that provide maximum biomass production of strain CPA-2 of the Biocontrol Agent Pantoea agglomerans and minimum cost of media, whilst maintaining Biocontrol efficacy. To reduce the cost of media, commercial products and by-products were tested. P. agglomerans can be produced using a combination of nitrogen sources such as yeast extract (5 g l–1) and dry beer yeast (10 g l–1) with inexpensive carbohydrates such as sucrose (10 g l–1) and molasses (20 g l–1), respectively, maintaining the efficacy of the Biocontrol Agent against Penicillium digitatum and P. italicum on oranges. The results obtained in this study could be used to provide a reliable basis for a scale-up of this fermentation process to an industrial level.

Florence Mathieu - One of the best experts on this subject based on the ideXlab platform.

  • Streptomyces roseolus, A Promising Biocontrol Agent Against Aspergillus flavus, the Main Aflatoxin B1 Producer
    Toxins, 2018
    Co-Authors: Isaura Caceres, Selma Snini, Olivier Puel, Florence Mathieu
    Abstract:

    Crop contamination by aflatoxin B1 is a current problem in tropical and subtropical regions. In the future, this contamination risk may be expanded to European countries due to climate change. The development of alternative strategies to prevent mycotoxin contamination that further contribute to the substitution of phytopharmaceutical products are thus needed. For this, a promising method resides in the use of Biocontrol Agents. Several actinobacteria strains have demonstrated to effectively reduce the aflatoxin B1 concentration. Nevertheless, the molecular mechanism of action by which these biological Agents reduce the mycotoxin concentration has not been determined. The aim of the present study was to test the potential use of Streptomyces roseolus as a Biocontrol Agent against aflatoxin B1 contamination. Co-cultures with Aspergillus flavus were conducted, and the molecular fungal response was investigated through analyzing the q-PCR expression of 65 genes encoding relevant fungal functions. Moreover, kojic and cyclopiazonic acid concentrations, as well as morphological fungal changes were also analyzed. The results demonstrated that reduced concentrations of aflatoxin B1 and kojic acid were respectively correlated with the down-regulation of the aflatoxin B1 gene cluster and kojR gene expression. Moreover, a fungal hypersporulated phenotype and a general over-expression of genes involved in fungal development were observed in the co-culture condition