The Experts below are selected from a list of 1548 Experts worldwide ranked by ideXlab platform
Kenji Kano - One of the best experts on this subject based on the ideXlab platform.
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Direct electron transfer-type Bioelectrocatalysis by membrane-bound aldehyde dehydrogenase from Gluconobacter oxydans and cyanide effects on its bioelectrocatalytic properties
Electrochemistry Communications, 2021Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Abstract The bioelectrocatalytic properties of membrane-bound aldehyde dehydrogenase (AlDH) from Gluconobacter oxydans NBRC12528 were evaluated. AlDH exhibited direct electron transfer (DET)-type bioelectrocatalytic activity for acetaldehyde oxidation at several kinds of electrodes. The kinetic and thermodynamic parameters for bioelectrocatalytic acetaldehyde oxidation were estimated based on the partially random orientation model. Moreover, at the multi-walled carbon nanotube-modified electrode, the coordination of CN− to AlDH switched the direction of the DET-type Bioelectrocatalysis to acetate reduction under acidic conditions. These phenomena were discussed from a thermodynamic viewpoint.
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Recent Progress in Applications of Enzymatic Bioelectrocatalysis
Catalysts, 2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis has become one of the most important research fields in electrochemistry and provided a firm base for the application of important technology in various bioelectrochemical devices, such as biosensors, biofuel cells, and biosupercapacitors. The understanding and technology of Bioelectrocatalysis have greatly improved with the introduction of nanostructured electrode materials and protein-engineering methods over the last few decades. Recently, the electroenzymatic production of renewable energy resources and useful organic compounds (bioelectrosynthesis) has attracted worldwide attention. In this review, we summarize recent progress in the applications of enzymatic Bioelectrocatalysis.
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Recent Progress in Applications of Enzymatic Bioelectrocatalysis
2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis has become one of important research fields in electrochemistry and provided a firm base for an important technology for application to various bioelectrochemical devices such as biosensors, biofuel cells, and biosupercapacitors. The understanding and technology in Bioelectrocatalysis have been greatly improved by introducing nanostructured electrode materials and protein-engineering methods over the last few decades. Recently, the electro-enzymatic production of renewable energy resources and useful organic compounds (bioelectrosynthesis) also attracts worldwide attention. In this review, we summarize recent progress in applications of enzymatic Bioelectrocatalysis.
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Development Perspective of Bioelectrocatalysis-Based Biosensors.
Sensors (Basel Switzerland), 2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
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Development Perspective of Bioelectrocatalysis-based Biosensors
2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis provides the intrinsic catalytic-functions of redox enzymes to non-specific electrode reactions and is the most important and basic concept for biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multi-analyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
Tokuji Ikeda - One of the best experts on this subject based on the ideXlab platform.
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Bioelectrocatalysis-based application of quinoproteins and quinoprotein-containing bacterial cells in biosensors and biofuel cells.
Biochimica et biophysica acta, 2003Co-Authors: Tokuji Ikeda, Kenji KanoAbstract:Electrochemical studies on the applied aspects of quinoproteins are briefly reviewed. Catalytic reactions of quinoprotein enzymes can be connected to electrochemical reactions directly or by the mediation of molecules functioning as electron acceptors of the enzymes. Such an enzyme-electrochemical reaction is called Bioelectrocatalysis. It provides a novel method of kinetic analysis of enzyme catalysis and even whole bacterial cell catalysis. The principle of Bioelectrocatalysis is first described, then, the Bioelectrocatalysis-based application of quinoproteins in biosensors is mentioned. Characteristics and performance of this type of biosensor is explained by citing our own work. Possible application in bioreactors and biofuel cells is also mentioned.
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Theory of steady-state catalytic current of mediated Bioelectrocatalysis
Journal of Electroanalytical Chemistry, 2002Co-Authors: Ryuhei Matsumoto, Kenji Kano, Tokuji IkedaAbstract:An analytical equation for the steady-state catalytic current of mediated Bioelectrocatalysis has been derived as a function of the mediator concentration and the electrode potential for the case where the concentration of a substrate is sufficiently higher than the Michaelis constant for the substrate and the redox reaction of a mediator to obey the Nernst equation at an electrode surface. The equation can be reduced to the equations so far proposed for limited cases. The equation representing the limiting steady-state catalytic current as a function of the mediator concentration is useful to evaluate the catalytic constant of the enzyme and the Michaelis constant for the mediator, separately.
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Approximate and empirical expression of the steady-state catalytic current of mediated Bioelectrocatalysis to evaluate enzyme kinetics
Journal of Electroanalytical Chemistry, 2001Co-Authors: Takanori Ohgaru, Kenji Kano, Hirosuke Tatsumi, Tokuji IkedaAbstract:An approximate equation is derived for the steady-state limiting current of mediated Bioelectrocatalysis as a function of the bulk mediator concentration in the case where enzyme and mediator are in the soluble state and the substrate concentration is sufficiently higher than the Michaelis constant of the substrate. The error of the approximate equation has been evaluated by digital simulation and an empirical equation to correct the error has been proposed to describe the steady-state current more precisely. These equations have been applied successfully to typical mediated bioelectrocatalytic systems to evaluate the catalytic constant and the Michaelis constant of the mediator.
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Fundamentals and Practices of Mediated Bioelectrocatalysis
Analytical Sciences, 2000Co-Authors: Kenji Kano, Tokuji IkedaAbstract:Recent progress in mediated Bioelectrocatalysis is reviewed. In a section concerning fundamentals, special attention is paid to describing the property of a steady-state current in this system from thermodynamic and kinetic viewpoints. Practical applications of the system includes biosensors for enzyme substrates, amplified detection of mediators, bioreactors, biofuel cells, enzyme kinetic measurements, and protein redox potential measurements.
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Mediated Bioelectrocatalysis based on nad-related enzymes with reversible characteristics
Journal of Electroanalytical Chemistry, 1998Co-Authors: Kazuyoshi Takagi, Kenji Kano, Tokuji IkedaAbstract:Diaphorase (DI) works as an effective catalyst for the electrochemical oxidation and reduction of NAD with the aid of several quinones or flavins as electron transfer mediators. The redox kinetics between DI and mediators have been expressed by a Butler–Volmer-type equation. NAD-dependent l-lactate dehydrogenase (LDH) catalyzing the redox reaction between l-lactate and pyruvate was coupled to the DI-catalyzed NAD redox system to achieve better understanding of mediated two-enzyme-linked Bioelectrocatalysis with reversible characteristics. Under the conditions where the concentration polarization of NAD due to the DI-catalyzed electrochemical reaction is suppressed by the LDH reaction, the NAD concentration dependence of the catalytic current was expressed by an approximate equation involving the enzyme kinetics between DI and NAD. The suppression of the NAD concentration polarization is also useful to observe steady-state catalytic waves of an uphill reaction between DI and the mediator. The oxidation reaction involving the uphill electron transfer from l-lactate to NAD+ is susceptible to a inhibition from pyruvate due to the reversible characteristics of LDH. The present knowledge has led to the strategy to realize a two-way Bioelectrocatalysis for the reduction of pyruvate and the oxidation of l-lactate. New potentiometry for the detection of the solution potential governed by the electrochemically inactive pyruvate/l-lactate redox couple has also been demonstrated based on the reversible characteristics of the DI–DLH-linked bioelectrocatalytic system.
Yuki Kitazumi - One of the best experts on this subject based on the ideXlab platform.
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Direct electron transfer-type Bioelectrocatalysis by membrane-bound aldehyde dehydrogenase from Gluconobacter oxydans and cyanide effects on its bioelectrocatalytic properties
Electrochemistry Communications, 2021Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Abstract The bioelectrocatalytic properties of membrane-bound aldehyde dehydrogenase (AlDH) from Gluconobacter oxydans NBRC12528 were evaluated. AlDH exhibited direct electron transfer (DET)-type bioelectrocatalytic activity for acetaldehyde oxidation at several kinds of electrodes. The kinetic and thermodynamic parameters for bioelectrocatalytic acetaldehyde oxidation were estimated based on the partially random orientation model. Moreover, at the multi-walled carbon nanotube-modified electrode, the coordination of CN− to AlDH switched the direction of the DET-type Bioelectrocatalysis to acetate reduction under acidic conditions. These phenomena were discussed from a thermodynamic viewpoint.
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Recent Progress in Applications of Enzymatic Bioelectrocatalysis
Catalysts, 2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis has become one of the most important research fields in electrochemistry and provided a firm base for the application of important technology in various bioelectrochemical devices, such as biosensors, biofuel cells, and biosupercapacitors. The understanding and technology of Bioelectrocatalysis have greatly improved with the introduction of nanostructured electrode materials and protein-engineering methods over the last few decades. Recently, the electroenzymatic production of renewable energy resources and useful organic compounds (bioelectrosynthesis) has attracted worldwide attention. In this review, we summarize recent progress in the applications of enzymatic Bioelectrocatalysis.
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Recent Progress in Applications of Enzymatic Bioelectrocatalysis
2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis has become one of important research fields in electrochemistry and provided a firm base for an important technology for application to various bioelectrochemical devices such as biosensors, biofuel cells, and biosupercapacitors. The understanding and technology in Bioelectrocatalysis have been greatly improved by introducing nanostructured electrode materials and protein-engineering methods over the last few decades. Recently, the electro-enzymatic production of renewable energy resources and useful organic compounds (bioelectrosynthesis) also attracts worldwide attention. In this review, we summarize recent progress in applications of enzymatic Bioelectrocatalysis.
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Development Perspective of Bioelectrocatalysis-Based Biosensors.
Sensors (Basel Switzerland), 2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
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Development Perspective of Bioelectrocatalysis-based Biosensors
2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis provides the intrinsic catalytic-functions of redox enzymes to non-specific electrode reactions and is the most important and basic concept for biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multi-analyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
Osamu Shirai - One of the best experts on this subject based on the ideXlab platform.
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Direct electron transfer-type Bioelectrocatalysis by membrane-bound aldehyde dehydrogenase from Gluconobacter oxydans and cyanide effects on its bioelectrocatalytic properties
Electrochemistry Communications, 2021Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Abstract The bioelectrocatalytic properties of membrane-bound aldehyde dehydrogenase (AlDH) from Gluconobacter oxydans NBRC12528 were evaluated. AlDH exhibited direct electron transfer (DET)-type bioelectrocatalytic activity for acetaldehyde oxidation at several kinds of electrodes. The kinetic and thermodynamic parameters for bioelectrocatalytic acetaldehyde oxidation were estimated based on the partially random orientation model. Moreover, at the multi-walled carbon nanotube-modified electrode, the coordination of CN− to AlDH switched the direction of the DET-type Bioelectrocatalysis to acetate reduction under acidic conditions. These phenomena were discussed from a thermodynamic viewpoint.
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Recent Progress in Applications of Enzymatic Bioelectrocatalysis
Catalysts, 2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis has become one of the most important research fields in electrochemistry and provided a firm base for the application of important technology in various bioelectrochemical devices, such as biosensors, biofuel cells, and biosupercapacitors. The understanding and technology of Bioelectrocatalysis have greatly improved with the introduction of nanostructured electrode materials and protein-engineering methods over the last few decades. Recently, the electroenzymatic production of renewable energy resources and useful organic compounds (bioelectrosynthesis) has attracted worldwide attention. In this review, we summarize recent progress in the applications of enzymatic Bioelectrocatalysis.
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Recent Progress in Applications of Enzymatic Bioelectrocatalysis
2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis has become one of important research fields in electrochemistry and provided a firm base for an important technology for application to various bioelectrochemical devices such as biosensors, biofuel cells, and biosupercapacitors. The understanding and technology in Bioelectrocatalysis have been greatly improved by introducing nanostructured electrode materials and protein-engineering methods over the last few decades. Recently, the electro-enzymatic production of renewable energy resources and useful organic compounds (bioelectrosynthesis) also attracts worldwide attention. In this review, we summarize recent progress in applications of enzymatic Bioelectrocatalysis.
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Development Perspective of Bioelectrocatalysis-Based Biosensors.
Sensors (Basel Switzerland), 2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
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Development Perspective of Bioelectrocatalysis-based Biosensors
2020Co-Authors: Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji KanoAbstract:Bioelectrocatalysis provides the intrinsic catalytic-functions of redox enzymes to non-specific electrode reactions and is the most important and basic concept for biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multi-analyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
Shelley D. Minteer - One of the best experts on this subject based on the ideXlab platform.
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Advancing the fundamental understanding and practical applications of photo-Bioelectrocatalysis.
Chemical communications (Cambridge England), 2020Co-Authors: Matteo Grattieri, Fangyuan Dong, Kevin Beaver, Erin M. Gaffney, Shelley D. MinteerAbstract:Photo-Bioelectrocatalysis combines the natural and highly sophisticated process of photosynthesis in biological entities with an abiotic electrode surface, to perform semi-artificial photosynthesis. However, challenges must be overcome, from the establishment and understanding of the photoexcited electron harvesting process at the electrode to the electrochemical characterization of these biotic/abiotic systems, and their subsequent tuning for enhancing energy generation (chemical and/or electrical). This Feature Article discusses the various approaches utilized to tackle these challenges, particularly focusing on powerful multi-disciplinary approaches for understanding and improving photo-Bioelectrocatalysis. Among them is the combination of experimental evidence and quantum mechanical calculations, the use of bioinformatics to understand photo-Bioelectrocatalysis at a metabolic level, or bioengineering to improve and facilitate photo-Bioelectrocatalysis. Key aspects for the future development of photo-Bioelectrocatalysis are presented alongside future research needs and promising applications of semi-artificial photosynthesis.
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The progress and outlook of Bioelectrocatalysis for the production of chemicals, fuels and materials
Nature Catalysis, 2020Co-Authors: Hui Chen, Fangyuan Dong, Shelley D. MinteerAbstract:Bioelectrocatalysis is a green, sustainable, efficient method to produce value-added chemicals, clean biofuels and degradable materials. As an alternative approach to modern biomanufacturing technology, Bioelectrocatalysis fully combines the merits of both biocatalysis and electrocatalysis to realize the green, efficient production of target products from electricity. Here, we review the development status of Bioelectrocatalysis, discussing the current challenges and looking toward future development directions. First, we detail the structure, function and modification methods of Bioelectrocatalysis. Next, we describe the mechanism of electron transfer, including mediated electron transfer and directed electron transfer. Third, we discuss the impact of the electrode on Bioelectrocatalysis. Then we analyse and summarize the application of Bioelectrocatalysis methods in the production of chemicals, biofuels and materials. Finally, we detail future developments and perspectives on Bioelectrocatalysis for electrosynthesis. Electrochemical reactions can provide necessary redox equivalents for biocatalysis. In this Review, Minteer and co-workers summarize the current status and challenges of enzymatic and microbial Bioelectrocatalysis for the green and efficient production of target products using electricity.
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Direct Bioelectrocatalysis by redox enzymes immobilized in electrostatically condensed oppositely charged polyelectrolyte electrode coatings
The Analyst, 2020Co-Authors: Koun Lim, Monika Sima, Russell J. Stewart, Shelley D. MinteerAbstract:The immobilization of enzymes on an electrode surface is critical in preserving enzyme activity and providing a sufficient electron transfer pathway for Bioelectrocatalysis. Here, we present a novel single-step, cross-linker free immobilization for direct Bioelectrocatalysis using an ionic strength induced phase inversion of oppositely charged polyelectrolytes. Cationic poly-guanidinyl-propyl-methacrylate (pGPMA, PG) and anionic inorganic polyphosphate, sodium hexametaphosphate (P6) were used to make an electrostatically condensed phase (PGP6). A mixture of PGP6 and laccase (LAC) from Tramates versicolor or HRP (HRP) from Armoracia rusticana were deposited on the electrode surface and were submerged in DI water to form white porous electrode coatings. Each electrode showed a current generation corresponding to the respective substrates via direct Bioelectrocatalysis.
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Unveiling salinity effects on photo-Bioelectrocatalysis through combination of bioinformatics and electrochemistry.
Electrochimica acta, 2020Co-Authors: Erin M. Gaffney, Matteo Grattieri, Kevin Beaver, Jennie Pham, Caitlin Mccartney, Shelley D. MinteerAbstract:Little is known about the adaptation strategies utilized by photosynthetic microorganisms to cope with salinity changes happening in the environment, and the effects on microbial electrochemical technologies. Herein, bioinformatics analysis revealed a metabolism shift in Rhodobacter capsulatus resulting from salt stress, with changes in gene expression allowing accumulation of compatible solutes to balance osmotic pressure, together with the up-regulation of the nitrogen fixation cycle, an electron sink of the photosynthetic electron transfer chain. Using the transcriptome evidence of hindered electron transfer in the photosynthetic electron transport chain induced by adaption to salinity, increased understanding of photo-Bioelectrocatalysis under salt stress is achieved. Accumulation of glycine-betaine allows immediate tuning of salinity tolerance but does not provide cell stabilization, with a 40 ± 20% loss of photo-Bioelectrocatalysis in a 60 min time scale. Conversely, exposure to or inducing the expression of the Rhodobacter capsulatus gene transfer agent tunes salinity tolerance and increases cell stability. This work provides a proof of concept for the combination of bioinformatics and electrochemical tools to investigate microbial electrochemical systems, opening exciting future research opportunities.
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Nitrogenase Bioelectrocatalysis: From Understanding Electron-Transfer Mechanisms to Energy Applications
ACS Energy Letters, 2018Co-Authors: Rong Cai, Shelley D. MinteerAbstract:Nitrogenase is the only enzyme known to reduce molecular nitrogen to ammonia, so it has been an oxidoreductase enzyme of great interest to the electrofuels and catalysis communities, as people consider the design of bioinspired nitrogen reduction catalysts for the production of ammonia. This Perspective details the use of nitrogenase as an electrocatalyst to learn mechanistic information about the electron-transfer mechanism as an inspiration for molecular and metal-based electrocatalysts and for a variety of energy applications, including energy-efficient ammonia production and biofuel cells. This Perspective also addresses the issues and challenges of nitrogenase Bioelectrocatalysis that will need to be addressed in the coming years.